RchiOBHm_Chr3g0455071

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0019914
Physical Location & Seq
Forward (+)
4936802 .. 4938164
1363 bp
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UTR
Exon/CDS
Intron
PRQ42205

Sequence Viewer

Length: 660 bp
ATGCTGAATCTGGTCAGTTTTCTCATCTATTTTAACTTTCACAAACGGGTTGCAAGGGAAAGTTGGAAAGGCAGCATACTTGGAAAACTGGTTCGCACAGTCACACCCATAACAGCAAGAGAGTCCGTTTTTAATGTCACCTTTGACTGGGAAGAGGAAATCAGAGTTCCAGGAGCGTTCTTGATAAGAAACAATCATCACAGTGAGTTTTATCTTAAGACTGTCACACTCGAAGATGTTCCGGATGAGGGCAGGATCCACTGTTTACAACTCTTGGATTCAAACAGCGAGAGTAGGTTGAACCTTCTTCTGAGCCTAAACATTTATGTTCCGAGAGATGAACGATTTGGCCACTTGAAGTTGGCAGACTTTCTTACTTATGGTTTAAAATCCATAGCTCAGTTCGTTAGAACAGGGCTTGAGGCTTTGTCTGATGATACTCCCAACGAGTTTGACAGCTTCGAAGATGTGCTTAAACTTTATGAAGGAGGGATTCCTTTGCCTCAAGGTTTATTAAAGGATATTGGGGACAACATCCCAGTGGAGATGCTCAAGGAAATTCTACGAACCGATGGTGGTGAAAATTTCCTCAGGTTCCCAGTGCCTCAAGTGATCAAAGAGGATATGACTGCTCGGAGGACTAATGAAGAATTTGCCTGA

Protein Analysis

219

Amino Acids

25.19

Weight (kDa)

5.12

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAT PF01477 35 - 86 4.1e-06 PLAT/LH2 domain
Lipoxygenase PF00305 92 - 219 2.2e-25 Lipoxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0019914)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0455071
rosa_samantha Rh3AG063800 Rh3AG064400 Rh3BG066200 Rh3BG066300 Rh3CG064600 Rh3CG064700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 241
AclWI GGATC 2 cut(s) 250, 263
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 3 cut(s) 558, 583, 650
AfiI CCNNNNNNNGG 2 cut(s) 147, 248
AflII CTTAAG 1 cut(s) 215
AgsI TTSAA 3 cut(s) 282, 301, 358
AjnI CCWGG 1 cut(s) 169
AloI GAACNNNNNNTCC 2 cut(s) 75, 107
AluBI AGCT 2 cut(s) 398, 459
AluI AGCT 2 cut(s) 398, 459
AlwI GGATC 2 cut(s) 250, 263
Aor13HI TCCGGA 1 cut(s) 241
AoxI GGCC 1 cut(s) 349
ApeKI GCWGC 1 cut(s) 72
ApoI RAATTY 3 cut(s) 558, 583, 650
Asp700I GAANNNNTTC 1 cut(s) 237
AsuHPI GGTGA 2 cut(s) 130, 590
AsuII TTCGAA 1 cut(s) 462
AxyI CCTNAGG 1 cut(s) 590
BalI TGGCCA 1 cut(s) 351
BamHI GGATCC 1 cut(s) 255
BbvI GCAGC 1 cut(s) 84
BccI CCATC 1 cut(s) 566
BciT130I CCWGG 1 cut(s) 171
BclI TGATCA 1 cut(s) 612
BfrI CTTAAG 1 cut(s) 215
BisI GCNGC 1 cut(s) 73
BlsI GCNGC 1 cut(s) 74
Bme1390I CCNGG 1 cut(s) 171
BmiI GGNNCC 2 cut(s) 257, 596
BmrFI CCNGG 1 cut(s) 171
BmrI ACTGGG 3 cut(s) 157, 533, 593
BmsI GCATC 1 cut(s) 537
BmuI ACTGGG 3 cut(s) 157, 533, 593
Bpu14I TTCGAA 1 cut(s) 462
BpuEI CTTGAG 4 cut(s) 440, 489, 536, 591
BsaWI WCCGGW 1 cut(s) 241
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 248
Bse1I ACTGG 4 cut(s) 93, 152, 539, 599
Bse21I CCTNAGG 1 cut(s) 590
BseAI TCCGGA 1 cut(s) 241
BseBI CCWGG 1 cut(s) 171
BseGI GGATG 2 cut(s) 250, 534
BseLI CCNNNNNNNGG 2 cut(s) 147, 248
BseMII CTCAG 3 cut(s) 302, 413, 604
BseNI ACTGG 4 cut(s) 93, 152, 539, 599
BseXI GCAGC 1 cut(s) 84
BshFI GGCC 1 cut(s) 351
BsiSI CCGG 1 cut(s) 242
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 2 cut(s) 147, 248
BsmFI GGGAC 1 cut(s) 542
BsnI GGCC 1 cut(s) 351
Bsp119I TTCGAA 1 cut(s) 462
Bsp13I TCCGGA 1 cut(s) 241
Bsp143I GATC 2 cut(s) 255, 612
BspANI GGCC 1 cut(s) 351
BspCNI CTCAG 3 cut(s) 303, 412, 603
BspEI TCCGGA 1 cut(s) 241
BspLI GGNNCC 2 cut(s) 257, 596
BspPI GGATC 2 cut(s) 250, 263
BspT104I TTCGAA 1 cut(s) 462
BspTI CTTAAG 1 cut(s) 215
BsrI ACTGG 4 cut(s) 93, 152, 539, 599
BssMI GATC 2 cut(s) 255, 612
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 4 cut(s) 100, 203, 223, 263
Bst6I CTCTTC 1 cut(s) 147
BstAFI CTTAAG 1 cut(s) 215
BstBI TTCGAA 1 cut(s) 462
BstDEI CTNAG 3 cut(s) 311, 399, 590
BstF5I GGATG 2 cut(s) 250, 534
BstKTI GATC 2 cut(s) 258, 615
BstMBI GATC 2 cut(s) 255, 612
BstNI CCWGG 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 169
BstV1I GCAGC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
Bsu36I CCTNAGG 1 cut(s) 590
BsuRI GGCC 1 cut(s) 351
BtsCI GGATG 2 cut(s) 250, 534
BtsIMutI CAGTG 4 cut(s) 208, 259, 546, 606
CviJI RGCY 6 cut(s) 315, 351, 398, 418, 425, 459
CviKI_1 RGCY 6 cut(s) 315, 351, 398, 418, 425, 459
DdeI CTNAG 3 cut(s) 311, 399, 590
DpnI GATC 2 cut(s) 257, 614
DpnII GATC 2 cut(s) 255, 612
DraI TTTAAA 1 cut(s) 387
EaeI YGGCCR 1 cut(s) 349
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Eco81I CCTNAGG 1 cut(s) 590
EcoRII CCWGG 1 cut(s) 169
FaiI YATR 7 cut(s) 77, 110, 327, 381, 395, 483, 626
FalI AAGNNNNNCTT 2 cut(s) 456, 488
FaqI GGGAC 1 cut(s) 542
FbaI TGATCA 1 cut(s) 612
Fnu4HI GCNGC 1 cut(s) 73
FokI GGATG 2 cut(s) 257, 521
Fsp4HI GCNGC 1 cut(s) 73
GluI GCNGC 1 cut(s) 73
HaeIII GGCC 1 cut(s) 351
HapII CCGG 1 cut(s) 242
HinfI GANTC 4 cut(s) 7, 122, 278, 493
HpaII CCGG 1 cut(s) 242
HphI GGTGA 2 cut(s) 130, 590
Hpy166II GTNNAC 1 cut(s) 266
Hpy188I TCNGA 5 cut(s) 164, 312, 333, 433, 636
Hpy188III TCNNGA 2 cut(s) 181, 242
Hpy8I GTNNAC 1 cut(s) 266
HpyAV CCTTC 2 cut(s) 314, 479
HpyCH4III ACNGT 4 cut(s) 100, 203, 223, 263
HpyCH4V TGCA 1 cut(s) 53
HpyF3I CTNAG 3 cut(s) 311, 399, 590
Kpn2I TCCGGA 1 cut(s) 241
Ksp22I TGATCA 1 cut(s) 612
Kzo9I GATC 2 cut(s) 255, 612
LmnI GCTCC 1 cut(s) 173
Lsp1109I GCAGC 1 cut(s) 84
LweI GCATC 1 cut(s) 537
MaeIII GTNAC 3 cut(s) 100, 136, 223
MalI GATC 2 cut(s) 257, 614
MboI GATC 2 cut(s) 255, 612
MboII GAAGA 5 cut(s) 164, 245, 299, 476, 659
MflI RGATCY 1 cut(s) 255
MlsI TGGCCA 1 cut(s) 351
MluCI AATT 3 cut(s) 558, 583, 650
MluNI TGGCCA 1 cut(s) 351
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 44
MnlI CCTC 9 cut(s) 148, 241, 415, 482, 513, 599, 613, 615, 630
Mox20I TGGCCA 1 cut(s) 351
MroI TCCGGA 1 cut(s) 241
MroXI GAANNNNTTC 1 cut(s) 237
MscI TGGCCA 1 cut(s) 351
MseI TTAA 6 cut(s) 33, 132, 216, 386, 474, 515
MslI CAYNNNNRTG 2 cut(s) 201, 539
Msp20I TGGCCA 1 cut(s) 351
MspCI CTTAAG 1 cut(s) 215
MspI CCGG 1 cut(s) 242
MspR9I CCNGG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 171
NdeII GATC 2 cut(s) 255, 612
NlaIV GGNNCC 2 cut(s) 257, 596
NmuCI GTSAC 3 cut(s) 100, 136, 223
NspV TTCGAA 1 cut(s) 462
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 3 cut(s) 7, 278, 493
PfoI TCCNGGA 1 cut(s) 169
PkrI GCNGC 1 cut(s) 74
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
Psp6I CCWGG 1 cut(s) 169
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 257, 596
PsuI RGATCY 1 cut(s) 255
RseI CAYNNNNRTG 2 cut(s) 201, 539
SaqAI TTAA 6 cut(s) 33, 132, 216, 386, 474, 515
SatI GCNGC 1 cut(s) 73
Sau3AI GATC 2 cut(s) 255, 612
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 171
SetI ASST 7 cut(s) 143, 299, 306, 400, 461, 511, 596
SfaNI GCATC 1 cut(s) 537
SfuI TTCGAA 1 cut(s) 462
SmiMI CAYNNNNRTG 2 cut(s) 201, 539
SmlI CTYRAG 5 cut(s) 215, 419, 504, 551, 606
SmoI CTYRAG 5 cut(s) 215, 419, 504, 551, 606
Sse9I AATT 3 cut(s) 558, 583, 650
StyD4I CCNGG 1 cut(s) 169
TaaI ACNGT 4 cut(s) 100, 203, 223, 263
TaqI TCGA 2 cut(s) 231, 462
TasI AATT 3 cut(s) 558, 583, 650
TfiI GAWTC 3 cut(s) 7, 278, 493
Tru1I TTAA 6 cut(s) 33, 132, 216, 386, 474, 515
Tru9I TTAA 6 cut(s) 33, 132, 216, 386, 474, 515
TscAI CASTG 4 cut(s) 208, 266, 546, 606
TseFI GTSAC 3 cut(s) 100, 136, 223
TseI GCWGC 1 cut(s) 72
Tsp45I GTSAC 3 cut(s) 100, 136, 223
TspDTI ATGAA 3 cut(s) 354, 498, 660
TspGWI ACGGA 1 cut(s) 115
TspRI CASTG 4 cut(s) 208, 266, 546, 606
Vha464I CTTAAG 1 cut(s) 215
XapI RAATTY 3 cut(s) 558, 583, 650
XmnI GAANNNNTTC 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.