Rh3BG066300

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0019914
Physical Location & Seq
Forward (+)
4462762 .. 4463962
1201 bp
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UTR
Exon/CDS
Intron
Rh3BG066300.1

Sequence Viewer

Length: 750 bp
ATGTCCGTCCTATTCTTGGAGGATCTAGAGAGTACATACCCTTACCCTCGAAGAGGAAGAACTGGACGAGCACCGACAAAGACAGATTCAAACAGAGACAGTAGGTTGAACCTTCTTCTGAGCCTAAACATTTATGTTCCGAGAGATGAACGATTTGGCCACTTGAAGTTGGCAGACTTTCTTACTTACGGTTTAAAATCCATAGCTCAGTTCTTTAGAACGGAGCTTGAGGCTTTCTTTGATGACACTCCCAACGAGTTTGACAGCTTCGAAGATGTGCTTAAACTTTATGAAGGAGGGATTCCTTTGCCTCAAAGTTTATTAAAGGATATTGGGGACAACATCCCAGTGGAGATGCTCAAGGAAATTCTACGAACCGATGGTGAAAATTTCCTCAGGTTCCCAGTGCCTCAAGTGATCAAAGAGGATAAGACTGCTTGGAGGACTAATGAAGAATTTGCCAGAGTAATGTTGGCTGGAGTTGACCCTGTCACCATTCAGCGTCTCCAAAAATTTCCACCAGCAAGCCAGCCGGATCCAACAGTTTACGGTGATCAAACCAGTACAATAACAGAACAGCACATAAAGGATAACTTGGAAGGGCTTTCAATAGATGAGGCAATCGAGAACAAGAAGCTATTCATATTAGATCATCATGATGCCTTTATGCCGTACCTGAGGCGTTATTTAGGACAGGAAGAGCAGAAGAGGGAAGGAAGAGAGCATTATGAATTGCCCTATTTGATCTAG

Protein Analysis

249

Amino Acids

28.97

Weight (kDa)

4.97

Isoelectric Point (pI)

52.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipoxygenase PF00305 11 - 234 1.8e-75 Lipoxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019914)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0455071
rosa_samantha Rh3AG063800 Rh3AG064400 Rh3BG066200 Rh3BG066300 Rh3CG064600 Rh3CG064700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 30, 530, 543
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 4 cut(s) 366, 388, 455, 512
AfaI GTAC 3 cut(s) 34, 565, 674
AfiI CCNNNNNNNGG 2 cut(s) 16, 53
AgsI TTSAA 4 cut(s) 90, 109, 166, 609
AluBI AGCT 4 cut(s) 206, 226, 267, 637
AluI AGCT 4 cut(s) 206, 226, 267, 637
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 2 cut(s) 90, 509
AlwI GGATC 3 cut(s) 30, 530, 543
AoxI GGCC 1 cut(s) 157
ApoI RAATTY 4 cut(s) 366, 388, 455, 512
Asp700I GAANNNNTTC 1 cut(s) 638
AsuHPI GGTGA 3 cut(s) 395, 484, 563
AsuII TTCGAA 1 cut(s) 270
AxyI CCTNAGG 2 cut(s) 395, 677
BalI TGGCCA 1 cut(s) 159
BamHI GGATCC 1 cut(s) 535
Bbv12I GWGCWC 1 cut(s) 73
BccI CCATC 1 cut(s) 374
BceAI ACGGC 1 cut(s) 655
BclI TGATCA 2 cut(s) 417, 553
BcoDI GTCTC 2 cut(s) 90, 509
BfaI CTAG 2 cut(s) 26, 748
BmiI GGNNCC 2 cut(s) 401, 537
BmrI ACTGGG 2 cut(s) 341, 398
BmsI GCATC 2 cut(s) 345, 649
BmuI ACTGGG 2 cut(s) 341, 398
BpmI CTGGAG 1 cut(s) 498
Bpu14I TTCGAA 1 cut(s) 270
BpuEI CTTGAG 3 cut(s) 248, 344, 396
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 53
Bse1I ACTGG 4 cut(s) 67, 347, 404, 561
Bse21I CCTNAGG 2 cut(s) 395, 677
BseGI GGATG 1 cut(s) 342
BseLI CCNNNNNNNGG 2 cut(s) 16, 53
BseMII CTCAG 4 cut(s) 110, 221, 409, 668
BseNI ACTGG 4 cut(s) 67, 347, 404, 561
BshFI GGCC 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 533
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 2 cut(s) 16, 53
BsmAI GTCTC 2 cut(s) 90, 509
BsmBI CGTCTC 1 cut(s) 509
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 1 cut(s) 159
Bsp119I TTCGAA 1 cut(s) 270
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 6 cut(s) 22, 417, 535, 553, 649, 744
BspANI GGCC 1 cut(s) 159
BspCNI CTCAG 4 cut(s) 111, 220, 408, 669
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 2 cut(s) 401, 537
BspPI GGATC 3 cut(s) 30, 530, 543
BspQI GCTCTTC 1 cut(s) 693
BspT104I TTCGAA 1 cut(s) 270
BsrI ACTGG 4 cut(s) 67, 347, 404, 561
BssMI GATC 6 cut(s) 22, 417, 535, 553, 649, 744
Bst4CI ACNGT 4 cut(s) 101, 191, 544, 551
Bst6I CTCTTC 4 cut(s) 46, 693, 701, 712
BstBI TTCGAA 1 cut(s) 270
BstC8I GCNNGC 2 cut(s) 526, 530
BstDEI CTNAG 4 cut(s) 119, 207, 395, 677
BstENI CCTNNNNNAGG 1 cut(s) 51
BstF5I GGATG 1 cut(s) 342
BstKTI GATC 6 cut(s) 25, 420, 538, 556, 652, 747
BstMAI GTCTC 2 cut(s) 90, 509
BstMBI GATC 6 cut(s) 22, 417, 535, 553, 649, 744
BstX2I RGATCY 2 cut(s) 22, 535
BstYI RGATCY 2 cut(s) 22, 535
Bsu36I CCTNAGG 2 cut(s) 395, 677
BsuRI GGCC 1 cut(s) 159
BtsCI GGATG 1 cut(s) 342
BtsIMutI CAGTG 2 cut(s) 354, 411
Cac8I GCNNGC 2 cut(s) 526, 530
CciI TCATGA 1 cut(s) 655
CseI GACGC 1 cut(s) 491
Csp6I GTAC 3 cut(s) 33, 564, 673
CviAII CATG 1 cut(s) 656
CviQI GTAC 3 cut(s) 33, 564, 673
DdeI CTNAG 4 cut(s) 119, 207, 395, 677
DpnI GATC 6 cut(s) 24, 419, 537, 555, 651, 746
DpnII GATC 6 cut(s) 22, 417, 535, 553, 649, 744
DraI TTTAAA 1 cut(s) 195
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 4 cut(s) 46, 693, 701, 712
EarI CTCTTC 4 cut(s) 46, 693, 701, 712
Eco81I CCTNAGG 2 cut(s) 395, 677
EcoNI CCTNNNNNAGG 1 cut(s) 51
Esp3I CGTCTC 1 cut(s) 509
FaeI CATG 1 cut(s) 659
FaiI YATR 9 cut(s) 37, 135, 203, 291, 584, 644, 657, 668, 729
FalI AAGNNNNNCTT 4 cut(s) 264, 296, 578, 610
FaqI GGGAC 1 cut(s) 350
FatI CATG 1 cut(s) 655
FbaI TGATCA 2 cut(s) 417, 553
FokI GGATG 1 cut(s) 329
FspBI CTAG 2 cut(s) 26, 748
GsuI CTGGAG 1 cut(s) 498
HaeIII GGCC 1 cut(s) 159
HapII CCGG 1 cut(s) 533
HgaI GACGC 1 cut(s) 491
Hin1II CATG 1 cut(s) 659
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HinfI GANTC 2 cut(s) 86, 301
HpaII CCGG 1 cut(s) 533
HphI GGTGA 3 cut(s) 395, 484, 563
Hpy166II GTNNAC 2 cut(s) 484, 547
Hpy188I TCNGA 2 cut(s) 120, 141
Hpy188III TCNNGA 3 cut(s) 26, 625, 656
Hpy8I GTNNAC 2 cut(s) 484, 547
HpyAV CCTTC 4 cut(s) 122, 287, 593, 707
HpyCH4III ACNGT 4 cut(s) 101, 191, 544, 551
HpyF3I CTNAG 4 cut(s) 119, 207, 395, 677
Hsp92II CATG 1 cut(s) 659
Ksp22I TGATCA 2 cut(s) 417, 553
Kzo9I GATC 6 cut(s) 22, 417, 535, 553, 649, 744
LguI GCTCTTC 1 cut(s) 693
LmnI GCTCC 1 cut(s) 223
LweI GCATC 2 cut(s) 345, 649
MaeI CTAG 2 cut(s) 26, 748
MaeIII GTNAC 1 cut(s) 490
MalI GATC 6 cut(s) 24, 419, 537, 555, 651, 746
MboI GATC 6 cut(s) 22, 417, 535, 553, 649, 744
MboII GAAGA 8 cut(s) 63, 69, 107, 284, 464, 710, 718, 729
MflI RGATCY 2 cut(s) 22, 535
MhlI GDGCHC 1 cut(s) 73
MlsI TGGCCA 1 cut(s) 159
MluCI AATT 5 cut(s) 366, 388, 455, 512, 731
MluNI TGGCCA 1 cut(s) 159
MmeI TCCRAC 1 cut(s) 563
Mox20I TGGCCA 1 cut(s) 159
MroXI GAANNNNTTC 1 cut(s) 638
MscI TGGCCA 1 cut(s) 159
MseI TTAA 3 cut(s) 194, 282, 323
MslI CAYNNNNRTG 2 cut(s) 347, 657
Msp20I TGGCCA 1 cut(s) 159
MspI CCGG 1 cut(s) 533
NdeII GATC 6 cut(s) 22, 417, 535, 553, 649, 744
NlaIII CATG 1 cut(s) 659
NlaIV GGNNCC 2 cut(s) 401, 537
NmuCI GTSAC 1 cut(s) 490
NspV TTCGAA 1 cut(s) 270
PagI TCATGA 1 cut(s) 655
PciSI GCTCTTC 1 cut(s) 693
PdmI GAANNNNTTC 1 cut(s) 638
PfeI GAWTC 2 cut(s) 86, 301
PflFI GACNNNGTC 1 cut(s) 488
PspN4I GGNNCC 2 cut(s) 401, 537
PsuI RGATCY 2 cut(s) 22, 535
PsyI GACNNNGTC 1 cut(s) 488
RsaI GTAC 3 cut(s) 34, 565, 674
RsaNI GTAC 3 cut(s) 33, 564, 673
RseI CAYNNNNRTG 2 cut(s) 347, 657
SapI GCTCTTC 1 cut(s) 693
SaqAI TTAA 3 cut(s) 194, 282, 323
Sau3AI GATC 6 cut(s) 22, 417, 535, 553, 649, 744
SduI GDGCHC 1 cut(s) 73
SetI ASST 8 cut(s) 107, 114, 208, 228, 269, 401, 639, 678
SfaNI GCATC 2 cut(s) 345, 649
SfuI TTCGAA 1 cut(s) 270
SmiMI CAYNNNNRTG 2 cut(s) 347, 657
SmlI CTYRAG 3 cut(s) 227, 359, 411
SmoI CTYRAG 3 cut(s) 227, 359, 411
Sse9I AATT 5 cut(s) 366, 388, 455, 512, 731
SspMI CTAG 2 cut(s) 26, 748
TaaI ACNGT 4 cut(s) 101, 191, 544, 551
TaqI TCGA 3 cut(s) 49, 270, 624
TasI AATT 5 cut(s) 366, 388, 455, 512, 731
TatI WGTACW 2 cut(s) 32, 563
TfiI GAWTC 2 cut(s) 86, 301
Tru1I TTAA 3 cut(s) 194, 282, 323
Tru9I TTAA 3 cut(s) 194, 282, 323
TscAI CASTG 2 cut(s) 354, 411
TseFI GTSAC 1 cut(s) 490
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 5 cut(s) 162, 306, 465, 631, 744
TspGWI ACGGA 1 cut(s) 236
TspRI CASTG 2 cut(s) 354, 411
Tth111I GACNNNGTC 1 cut(s) 488
XagI CCTNNNNNAGG 1 cut(s) 51
XapI RAATTY 4 cut(s) 366, 388, 455, 512
XbaI TCTAGA 1 cut(s) 25
XcmI CCANNNNNNNNNTGG 1 cut(s) 469
XmnI GAANNNNTTC 1 cut(s) 638
XspI CTAG 2 cut(s) 26, 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.