RchiOBHm_Chr3g0463441

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0018903
Physical Location & Seq
Reverse (-)
10771539 .. 10772519
981 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42976

Sequence Viewer

Length: 981 bp
ATGGGTCGTCTTCTACGCCTGCAACTGCTTCATCTGTCTCTCAATTCCTTCAGTGGGAAATTTCCATCTAATATATCTCACTGCACACAACTAAGAGTGCTCAATGTTATTTCCAATGAGCTTACTGGGTCGATCCCTGATCAACTTGGTTCATTATTGAAGTTAACTCATCTATGGCTTCAGGGTAACCATCTTACTGGAAAAATCCCAGAGTGGATAGGAAACTTTTCATCTTTGTACAGTATAGCTCTTGGCGACAACAATTTACAGGGAAGGATACCCAATCAGCTCGGGCGCCTAACAAGCCTGGGAAACTTCCTAGTTTGGAGCAATAATTTGTCTGGTATGGTCCCTTCTTCAATTTATATTATCATACACATTTTCAGTGTTACTGACAACCAGCTGCGTGGAGAGCTGCCGCAAAACGTTGGCATTACTCTTCCTAATTTGGAGGTATTTGCCGGCGATGTCAACAAATTCACAGGAACTGTTCCAGCATCATTGTCAAATGCTTCTAGACTTCGGCTTCTTGATTTTGCTGAAGACAATTCACTAGGAAGTGGGAAAGATGGTGACCTTACTTCTCTCAATTTCCTTGCTAATTGTACTAATCTTGAGGTGTTGAGTTTTAGCCGTAATCATCTTGGAGGAGAATTGCCAGATTCCATAGCTAACCTATCAACCAAACTGAGGATATTTACAATAGGAGGAAATCTGATATATGGACCCCTCCCTACTGGCATTGGAAATCTGGTAAACTTGACAACTCTTGGAATAGAACAAAACCACATTGGTGTTAGTCTCCCTGATGTGATCGGGAAGCTTTACAAGTTAGAGGGACTGTATCTGAATCTGAACATATTTTCAGGGCCAATCCCATCCTCCTTAGGTAACTTGACTTCAGTGACAAGGCTCTTCATGAAGGGGAATAGGTTTGAGGGAAGCATACCTCCTAGTCTTGGAAACTGCCAAAATCTTTGA

Protein Analysis

326

Amino Acids

35.24

Weight (kDa)

7.78

Isoelectric Point (pI)

28.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 2 - 106 4.7e-07 Leucine-rich repeat region
LRR_14 PF23598 229 - 305 1e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018903)

Species Orthologous Gene IDs
malus_domestica MD04G1150000.v1.1 MD04G1150200.v1.1 MD05G1075900.v1.1
prunus_persica Prupe.6G248200_v2.0.a1
pyrus_communis pycom12g11010
rosa_chinensis RchiOBHm_Chr3g0463441 RchiOBHm_Chr4g0408581
rosa_multiflora Rmu_sc0001053.1_g000011
rosa_roxburghii Rroxscaffold_4G00322890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 294
AciI CCGC 1 cut(s) 419
AclI AACGTT 1 cut(s) 426
AclWI GGATC 1 cut(s) 127
AcsI RAATTY 2 cut(s) 59, 476
AcuI CTGAAG 4 cut(s) 34, 164, 561, 885
AcyI GRCGYC 1 cut(s) 295
AfaI GTAC 2 cut(s) 239, 607
AfiI CCNNNNNNNGG 3 cut(s) 54, 690, 959
AgsI TTSAA 2 cut(s) 160, 360
AjnI CCWGG 1 cut(s) 306
AleI CACNNNNGTG 1 cut(s) 792
AluBI AGCT 7 cut(s) 121, 248, 289, 403, 415, 671, 823
AluI AGCT 7 cut(s) 121, 248, 289, 403, 415, 671, 823
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 2 cut(s) 42, 806
AlwI GGATC 1 cut(s) 127
AlwNI CAGNNNCTG 1 cut(s) 488
Ama87I CYCGRG 1 cut(s) 290
AoxI GGCC 1 cut(s) 869
ApeKI GCWGC 2 cut(s) 403, 415
ApoI RAATTY 2 cut(s) 59, 476
Asp700I GAANNNNTTC 1 cut(s) 226
AspLEI GCGC 1 cut(s) 297
AspS9I GGNCC 3 cut(s) 349, 725, 869
AsuHPI GGTGA 1 cut(s) 584
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 2 cut(s) 349, 725
AxyI CCTNAGG 1 cut(s) 886
BanI GGYRCC 1 cut(s) 294
BarI GAAGNNNNNNTAC 4 cut(s) 337, 369, 883, 915
BbsI GAAGAC 2 cut(s) 2, 549
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 2 cut(s) 390, 402
BccI CCATC 4 cut(s) 73, 198, 563, 886
BceAI ACGGC 1 cut(s) 618
BciT130I CCWGG 1 cut(s) 308
BciVI GTATCC 1 cut(s) 270
BclI TGATCA 1 cut(s) 139
BcoDI GTCTC 2 cut(s) 42, 806
BfaI CTAG 4 cut(s) 320, 516, 554, 954
BfoI RGCGCY 1 cut(s) 298
BfuI GTATCC 1 cut(s) 270
BisI GCNGC 3 cut(s) 404, 416, 419
BlsI GCNGC 3 cut(s) 405, 417, 420
Bme1390I CCNGG 1 cut(s) 308
Bme18I GGWCC 2 cut(s) 349, 725
BmeT110I CYCGRG 1 cut(s) 290
BmgT120I GGNCC 3 cut(s) 349, 725, 869
BmiI GGNNCC 3 cut(s) 296, 351, 727
BmrFI CCNGG 1 cut(s) 308
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 506
BmuI ACTGGG 1 cut(s) 135
BpiI GAAGAC 2 cut(s) 2, 549
BpuEI CTTGAG 1 cut(s) 635
BsaHI GRCGYC 1 cut(s) 295
BsaJI CCNNGG 1 cut(s) 307
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 690, 959
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 3 cut(s) 130, 202, 742
Bse21I CCTNAGG 1 cut(s) 886
BseBI CCWGG 1 cut(s) 308
BseDI CCNNGG 1 cut(s) 307
BseGI GGATG 1 cut(s) 878
BseLI CCNNNNNNNGG 3 cut(s) 54, 690, 959
BseMII CTCAG 1 cut(s) 680
BseNI ACTGG 3 cut(s) 130, 202, 742
BseRI GAGGAG 1 cut(s) 663
BseXI GCAGC 2 cut(s) 390, 402
BsgI GTGCAG 1 cut(s) 67
BshFI GGCC 1 cut(s) 871
BshNI GGYRCC 1 cut(s) 294
BsiHKAI GWGCWC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 1 cut(s) 462
BslFI GGGAC 2 cut(s) 335, 852
BslI CCNNNNNNNGG 3 cut(s) 54, 690, 959
BsmAI GTCTC 2 cut(s) 42, 806
BsmFI GGGAC 2 cut(s) 335, 852
BsnI GGCC 1 cut(s) 871
BsoBI CYCGRG 1 cut(s) 290
Bsp1286I GDGCHC 1 cut(s) 102
Bsp1407I TGTACA 1 cut(s) 237
Bsp143I GATC 3 cut(s) 132, 139, 813
BspACI CCGC 1 cut(s) 419
BspANI GGCC 1 cut(s) 871
BspCNI CTCAG 1 cut(s) 681
BspHI TCATGA 1 cut(s) 918
BspLI GGNNCC 3 cut(s) 296, 351, 727
BspPI GGATC 1 cut(s) 127
BspQI GCTCTTC 1 cut(s) 920
BspT107I GGYRCC 1 cut(s) 294
BsrFI RCCGGY 1 cut(s) 461
BsrGI TGTACA 1 cut(s) 237
BsrI ACTGG 3 cut(s) 130, 202, 742
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 1 cut(s) 307
BssMI GATC 3 cut(s) 132, 139, 813
BssNI GRCGYC 1 cut(s) 295
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 3 cut(s) 242, 490, 843
Bst6I CTCTTC 2 cut(s) 444, 920
BstACI GRCGYC 1 cut(s) 295
BstAUI TGTACA 1 cut(s) 237
BstC8I GCNNGC 2 cut(s) 20, 463
BstDEI CTNAG 3 cut(s) 92, 689, 886
BstEII GGTNACC 2 cut(s) 185, 572
BstF5I GGATG 1 cut(s) 878
BstH2I RGCGCY 1 cut(s) 298
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 3 cut(s) 135, 142, 816
BstMAI GTCTC 2 cut(s) 42, 806
BstMBI GATC 3 cut(s) 132, 139, 813
BstMWI GCNNNNNNNGC 2 cut(s) 303, 412
BstNI CCWGG 1 cut(s) 308
BstPI GGTNACC 2 cut(s) 185, 572
BstSCI CCNGG 1 cut(s) 306
BstV1I GCAGC 2 cut(s) 390, 402
BstV2I GAAGAC 2 cut(s) 2, 549
BstXI CCANNNNNNTGG 2 cut(s) 197, 407
Bsu36I CCTNAGG 1 cut(s) 886
BsuI GTATCC 1 cut(s) 270
BsuRI GGCC 1 cut(s) 871
BtgZI GCGATG 1 cut(s) 480
BtsCI GGATG 1 cut(s) 878
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 4 cut(s) 58, 79, 391, 909
Cac8I GCNNGC 2 cut(s) 20, 463
CaiI CAGNNNCTG 1 cut(s) 488
CciI TCATGA 1 cut(s) 918
CfoI GCGC 1 cut(s) 297
Cfr10I RCCGGY 1 cut(s) 461
Cfr13I GGNCC 3 cut(s) 349, 725, 869
Csp6I GTAC 2 cut(s) 238, 606
CviAII CATG 1 cut(s) 919
CviQI GTAC 2 cut(s) 238, 606
DdeI CTNAG 3 cut(s) 92, 689, 886
DinI GGCGCC 1 cut(s) 296
DpnI GATC 3 cut(s) 134, 141, 815
DpnII GATC 3 cut(s) 132, 139, 813
Eam1104I CTCTTC 2 cut(s) 444, 920
EarI CTCTTC 2 cut(s) 444, 920
Eco47I GGWCC 2 cut(s) 349, 725
Eco57I CTGAAG 4 cut(s) 34, 164, 561, 885
Eco81I CCTNAGG 1 cut(s) 886
Eco88I CYCGRG 1 cut(s) 290
Eco91I GGTNACC 2 cut(s) 185, 572
EcoO65I GGTNACC 2 cut(s) 185, 572
EcoRII CCWGG 1 cut(s) 306
EgeI GGCGCC 1 cut(s) 296
EheI GGCGCC 1 cut(s) 296
FaeI CATG 1 cut(s) 922
FaqI GGGAC 2 cut(s) 335, 852
FatI CATG 1 cut(s) 918
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 3 cut(s) 404, 416, 419
FokI GGATG 1 cut(s) 865
Fsp4HI GCNGC 3 cut(s) 404, 416, 419
FspBI CTAG 4 cut(s) 320, 516, 554, 954
GlaI GCGC 1 cut(s) 296
GluI GCNGC 3 cut(s) 404, 416, 419
HaeII RGCGCY 1 cut(s) 298
HaeIII GGCC 1 cut(s) 871
HapII CCGG 1 cut(s) 462
HhaI GCGC 1 cut(s) 297
Hin1I GRCGYC 1 cut(s) 295
Hin1II CATG 1 cut(s) 922
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HincII GTYRAC 2 cut(s) 165, 472
HindII GTYRAC 2 cut(s) 165, 472
HindIII AAGCTT 1 cut(s) 821
HinfI GANTC 2 cut(s) 662, 850
HpaI GTTAAC 1 cut(s) 165
HpaII CCGG 1 cut(s) 462
HphI GGTGA 1 cut(s) 584
Hpy166II GTNNAC 3 cut(s) 165, 472, 757
Hpy188I TCNGA 3 cut(s) 717, 849, 855
Hpy188III TCNNGA 5 cut(s) 516, 530, 614, 817, 919
Hpy8I GTNNAC 3 cut(s) 165, 472, 757
HpyAV CCTTC 4 cut(s) 58, 267, 363, 916
HpyCH4III ACNGT 3 cut(s) 242, 490, 843
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 2 cut(s) 22, 84
HpyF10VI GCNNNNNNNGC 2 cut(s) 303, 412
HpyF3I CTNAG 3 cut(s) 92, 689, 886
HpySE526I ACGT 1 cut(s) 426
Hsp92I GRCGYC 1 cut(s) 295
Hsp92II CATG 1 cut(s) 922
HspAI GCGC 1 cut(s) 295
KasI GGCGCC 1 cut(s) 294
KroI GCCGGC 1 cut(s) 461
KroNI GCCGGC 1 cut(s) 463
Ksp22I TGATCA 1 cut(s) 139
KspAI GTTAAC 1 cut(s) 165
Kzo9I GATC 3 cut(s) 132, 139, 813
LguI GCTCTTC 1 cut(s) 920
LmnI GCTCC 1 cut(s) 327
Lsp1109I GCAGC 2 cut(s) 390, 402
LweI GCATC 1 cut(s) 506
MaeI CTAG 4 cut(s) 320, 516, 554, 954
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 5 cut(s) 185, 388, 572, 890, 904
MalI GATC 3 cut(s) 134, 141, 815
MboI GATC 3 cut(s) 132, 139, 813
MboII GAAGA 4 cut(s) 348, 431, 554, 907
MhlI GDGCHC 1 cut(s) 102
Mly113I GGCGCC 1 cut(s) 295
MroNI GCCGGC 1 cut(s) 461
MroXI GAANNNNTTC 1 cut(s) 226
MseI TTAA 1 cut(s) 164
MslI CAYNNNNRTG 1 cut(s) 792
MspA1I CMGCKG 1 cut(s) 403
MspI CCGG 1 cut(s) 462
MspR9I CCNGG 1 cut(s) 308
MvaI CCWGG 1 cut(s) 308
MwoI GCNNNNNNNGC 2 cut(s) 303, 412
NaeI GCCGGC 1 cut(s) 463
NarI GGCGCC 1 cut(s) 295
NdeII GATC 3 cut(s) 132, 139, 813
NgoMIV GCCGGC 1 cut(s) 461
NlaIII CATG 1 cut(s) 922
NlaIV GGNNCC 3 cut(s) 296, 351, 727
NmuCI GTSAC 2 cut(s) 572, 904
OliI CACNNNNGTG 1 cut(s) 792
PagI TCATGA 1 cut(s) 918
PciSI GCTCTTC 1 cut(s) 920
PdiI GCCGGC 1 cut(s) 463
PdmI GAANNNNTTC 1 cut(s) 226
PfeI GAWTC 2 cut(s) 662, 850
PkrI GCNGC 3 cut(s) 405, 417, 420
PluTI GGCGCC 1 cut(s) 298
Psp1406I AACGTT 1 cut(s) 426
Psp6I CCWGG 1 cut(s) 306
PspEI GGTNACC 2 cut(s) 185, 572
PspGI CCWGG 1 cut(s) 306
PspN4I GGNNCC 3 cut(s) 296, 351, 727
PspPI GGNCC 3 cut(s) 349, 725, 869
PstNI CAGNNNCTG 1 cut(s) 488
PvuII CAGCTG 1 cut(s) 403
RsaI GTAC 2 cut(s) 239, 607
RsaNI GTAC 2 cut(s) 238, 606
RseI CAYNNNNRTG 1 cut(s) 792
SapI GCTCTTC 1 cut(s) 920
SaqAI TTAA 1 cut(s) 164
SatI GCNGC 3 cut(s) 404, 416, 419
Sau3AI GATC 3 cut(s) 132, 139, 813
Sau96I GGNCC 3 cut(s) 349, 725, 869
ScrFI CCNGG 1 cut(s) 308
SduI GDGCHC 1 cut(s) 102
SfaNI GCATC 1 cut(s) 506
SfoI GGCGCC 1 cut(s) 296
SinI GGWCC 2 cut(s) 349, 725
SmiMI CAYNNNNRTG 1 cut(s) 792
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
SsiI CCGC 1 cut(s) 419
SspDI GGCGCC 1 cut(s) 294
SspMI CTAG 4 cut(s) 320, 516, 554, 954
StyD4I CCNGG 1 cut(s) 306
TaaI ACNGT 3 cut(s) 242, 490, 843
TaiI ACGT 1 cut(s) 429
TaqI TCGA 1 cut(s) 131
TatI WGTACW 2 cut(s) 237, 605
TauI GCSGC 1 cut(s) 421
TfiI GAWTC 2 cut(s) 662, 850
Tru1I TTAA 1 cut(s) 164
Tru9I TTAA 1 cut(s) 164
TscAI CASTG 4 cut(s) 58, 86, 391, 909
TseFI GTSAC 2 cut(s) 572, 904
TseI GCWGC 2 cut(s) 403, 415
Tsp45I GTSAC 2 cut(s) 572, 904
TspDTI ATGAA 5 cut(s) 20, 141, 219, 907, 935
TspRI CASTG 4 cut(s) 58, 86, 391, 909
VpaK11BI GGWCC 2 cut(s) 349, 725
XapI RAATTY 2 cut(s) 59, 476
XbaI TCTAGA 1 cut(s) 515
XmnI GAANNNNTTC 1 cut(s) 226
XspI CTAG 4 cut(s) 320, 516, 554, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.