RchiOBHm_Chr4g0408581

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0018903
Physical Location & Seq
Reverse (-)
31376028 .. 31381216
5189 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37966

Sequence Viewer

Length: 1629 bp
ATGAGCTCATGGAATGAATCCATGCATTTTTGCAGTTGGATAGGCACTAGAAGAGTCGTGACTTTGAACTTGCAAGCTCAAAAGTTGGCTGGCTCCGTACCACCTTCTATAGGGAATCTCACTTATCTCACTGGAATCAACTTGATAAACAACAACTTTTATAGTGCAGTTCCTCAGGAAATGGGTAGTCTCTTGCGCCTGCAATATCTCAACATGTCTCAAATCTCCTTCGGTGGGACAATTCTGACTAATATATCTCGCTGTACGCAGCTGACAATGATTGATGTTTTTTCCAATAAACTCATTAGGCCAATTCCCGACCAACTCAGTTTATTGTTGAAATTAACTGTGTTATTGATTGGTCGGAACAATCTCACTGGGACCATCCCACGTTGGATTGGGAACTTTTCTTCTTTGTTTGCACTTTCTCTTGGTGAAAACAACTTGCATGGAAGCATACCCAATGAGCTGGGGCGTCCAACTGCTTTGCAGAGATTCATACTTACCTCAAATAATCCTTCTGGTATGATTCCTTCTTCGATATATAACATTTCCTCCATATATTATTTCACTGTTACTCAGAATCAACTGCATGGGGAGCCCCCACAAAACATTGGCATTACTCTTCCTAATCTGGAGGTATTTGCCGGCGGTGTCAAGAAATTCACAGGAACTGTTCCAGCATCATTGTCAAATGCTTCTAGACTTCGGATTCTTGATTTTGCTCAAAATGGTCTCACTGGTAAACTCCCTGCTGAAAATCTTGGAAGGTTGAAATGGTTAATTAGAGTGAACTTTGACATGAACAGACTGGGAATAAGTGTCTTGAGGTGTTGGGTCTTAGCCGCAATCACTTTGGAGGAGAATTACCAGCCAGCATCCATAGCTAACCTTTCTACCCAGCTAAAAATTCTTACTCTGGGGGAAAATTTGATACATGGAAGCCTCCCTACTGGCATTGGAAATCTAGTAAACTTGACACTTCTGGGAATGGAAGGTTACCACATATCTAATAGTTTCCCTGATGCAATCGGGAAGCTTCACAAGTTAGAAGGTCTGGACTTGAATCTGAATAGATTTTCAGGGCCAATCCCATCCTCCCTAGGTAATTTGACTTCAGTGACAAGGCTCTTCATGGATGCTAATAGGTTTCAGGGATGCATACCTCCAAGTCTTGGGAACTGCCAAAATCTATTGATACTCAACCTTTCTAGTAACAATCTAAATGGCACAATACCTAGAGAGGTAGTGGCGATTAAATCCCTTTCGATTTCTTTGACCGTGTCTAACAATTCTTTGACTGGTTCACTACCATCGCCTACTAGAAAACTTGTCTTTTACGACGCGCAAATTACGGCGTGCATATCGTCTAGACATATTAGGAAACAAGTTATCGGTGAAATCCCCGAAACCCTTGGCAGTTGTATTAGTTTGCAGCGCCTGCTTTTGGAAGGTAATGAATTCAGCGGACATATTCCTCAGTCTATGGAAAAGCTAAGAGGCTTGGAAGAGTTGGATATTTCACGCAATAACTTATCTGGCCAGGTTCCTAAATTTGTAGGCAAGTTTCGAGCTCTCAAGTATCTCAATCTTTCTCAGAATGATTTTGAGGGTAAATTGCCTAACTAA

Protein Analysis

542

Amino Acids

59.39

Weight (kDa)

9.49

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 59 - 262 1.7e-07 Leucine-rich repeat region
LRR_8 PF13855 114 - 172 4.2e-06 Leucine rich repeat
LRR_14 PF23598 275 - 398 1.6e-08 Leucine-rich repeat region
LRR_8 PF13855 477 - 536 2.3e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018903)

Species Orthologous Gene IDs
malus_domestica MD04G1150000.v1.1 MD04G1150200.v1.1 MD05G1075900.v1.1
prunus_persica Prupe.6G248200_v2.0.a1
pyrus_communis pycom12g11010
rosa_chinensis RchiOBHm_Chr3g0463441 RchiOBHm_Chr4g0408581
rosa_multiflora Rmu_sc0001053.1_g000011
rosa_roxburghii Rroxscaffold_4G00322890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1175
AccII CGCG 1 cut(s) 1346
AciI CCGC 3 cut(s) 651, 846, 1467
AcoI YGGCCR 1 cut(s) 1540
AcsI RAATTY 5 cut(s) 662, 909, 928, 1460, 1553
AcuI CTGAAG 1 cut(s) 1101
AcyI GRCGYC 1 cut(s) 475
AfaI GTAC 2 cut(s) 99, 265
AfiI CCNNNNNNNGG 4 cut(s) 110, 234, 1175, 1447
AflIII ACRYGT 1 cut(s) 213
AgsI TTSAA 4 cut(s) 67, 340, 775, 1066
AjnI CCWGG 1 cut(s) 1542
AluBI AGCT 9 cut(s) 6, 77, 271, 469, 887, 904, 1039, 1495, 1574
AluI AGCT 9 cut(s) 6, 77, 271, 469, 887, 904, 1039, 1495, 1574
Alw21I GWGCWC 2 cut(s) 8, 1576
Alw26I GTCTC 3 cut(s) 194, 222, 740
AlwNI CAGNNNCTG 2 cut(s) 674, 1441
AoxI GGCC 3 cut(s) 308, 1085, 1540
ApeKI GCWGC 2 cut(s) 268, 1435
ApoI RAATTY 5 cut(s) 662, 909, 928, 1460, 1553
AspA2I CCTAGG 1 cut(s) 1102
AspLEI GCGC 3 cut(s) 198, 1348, 1440
AspS9I GGNCC 2 cut(s) 381, 1085
AsuHPI GGTGA 2 cut(s) 446, 1409
AvaII GGWCC 1 cut(s) 381
AvrII CCTAGG 1 cut(s) 1102
AxyI CCTNAGG 1 cut(s) 174
BalI TGGCCA 1 cut(s) 1542
BanII GRGCYC 3 cut(s) 8, 603, 1576
BarI GAAGNNNNNNTAC 6 cut(s) 517, 549, 934, 966, 1099, 1131
Bbv12I GWGCWC 2 cut(s) 8, 1576
BbvI GCAGC 2 cut(s) 280, 1447
BccI CCATC 3 cut(s) 392, 1102, 1321
BceAI ACGGC 1 cut(s) 1371
BciT130I CCWGG 1 cut(s) 1544
BcoDI GTCTC 3 cut(s) 194, 222, 740
BfaI CTAG 8 cut(s) 48, 702, 968, 1103, 1212, 1239, 1323, 1371
BfmI CTRYAG 1 cut(s) 108
BfoI RGCGCY 1 cut(s) 1441
BisI GCNGC 3 cut(s) 269, 846, 1436
BlnI CCTAGG 1 cut(s) 1102
BlsI GCNGC 3 cut(s) 270, 847, 1437
Bme1390I CCNGG 1 cut(s) 1544
Bme18I GGWCC 1 cut(s) 381
BmgT120I GGNCC 2 cut(s) 381, 1085
BmiI GGNNCC 4 cut(s) 94, 382, 600, 1548
BmrFI CCNGG 1 cut(s) 1544
BmrI ACTGGG 2 cut(s) 387, 821
BmsI GCATC 5 cut(s) 692, 887, 1015, 1129, 1148
BmuI ACTGGG 2 cut(s) 387, 821
BpmI CTGGAG 1 cut(s) 656
BpuEI CTTGAG 2 cut(s) 847, 1562
BsaHI GRCGYC 1 cut(s) 475
BsaI GGTCTC 1 cut(s) 740
BsaJI CCNNGG 2 cut(s) 1102, 1414
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bsc4I CCNNNNNNNGG 4 cut(s) 110, 234, 1175, 1447
Bse118I RCCGGY 1 cut(s) 647
Bse1I ACTGG 6 cut(s) 136, 382, 745, 816, 958, 1306
Bse21I CCTNAGG 1 cut(s) 174
BseBI CCWGG 1 cut(s) 1544
BseDI CCNNGG 2 cut(s) 1102, 1414
BseGI GGATG 5 cut(s) 384, 878, 1094, 1144, 1163
BseLI CCNNNNNNNGG 4 cut(s) 110, 234, 1175, 1447
BseMII CTCAG 5 cut(s) 188, 340, 593, 1493, 1610
BseNI ACTGG 6 cut(s) 136, 382, 745, 816, 958, 1306
BseRI GAGGAG 1 cut(s) 875
BseXI GCAGC 2 cut(s) 280, 1447
BseYI CCCAGC 2 cut(s) 469, 900
BsgI GTGCAG 1 cut(s) 186
Bsh1236I CGCG 1 cut(s) 1346
BshFI GGCC 3 cut(s) 310, 1087, 1542
BsiHKAI GWGCWC 2 cut(s) 8, 1576
BsiSI CCGG 1 cut(s) 648
BslFI GGGAC 2 cut(s) 250, 394
BslI CCNNNNNNNGG 4 cut(s) 110, 234, 1175, 1447
BsmAI GTCTC 3 cut(s) 194, 222, 740
BsmFI GGGAC 2 cut(s) 250, 394
BsnI GGCC 3 cut(s) 310, 1087, 1542
Bso31I GGTCTC 1 cut(s) 740
Bsp1286I GDGCHC 3 cut(s) 8, 603, 1576
BspACI CCGC 3 cut(s) 651, 846, 1467
BspANI GGCC 3 cut(s) 310, 1087, 1542
BspCNI CTCAG 5 cut(s) 187, 339, 592, 1492, 1609
BspFNI CGCG 1 cut(s) 1346
BspLI GGNNCC 4 cut(s) 94, 382, 600, 1548
BspQI GCTCTTC 1 cut(s) 1136
BspTNI GGTCTC 1 cut(s) 740
BsrFI RCCGGY 1 cut(s) 647
BsrI ACTGG 6 cut(s) 136, 382, 745, 816, 958, 1306
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 2 cut(s) 1102, 1414
BssNI GRCGYC 1 cut(s) 475
BssT1I CCWWGG 2 cut(s) 1102, 1414
Bst2UI CCWGG 1 cut(s) 1544
Bst4CI ACNGT 4 cut(s) 349, 574, 676, 1282
Bst6I CTCTTC 4 cut(s) 46, 630, 1136, 1503
BstACI GRCGYC 1 cut(s) 475
BstAPI GCANNNNNTGC 1 cut(s) 1441
BstC8I GCNNGC 7 cut(s) 75, 91, 200, 649, 876, 1360, 1442
BstDEI CTNAG 7 cut(s) 174, 326, 579, 841, 1479, 1496, 1596
BstEII GGTNACC 1 cut(s) 998
BstENI CCTNNNNNAGG 1 cut(s) 108
BstF5I GGATG 5 cut(s) 384, 878, 1094, 1144, 1163
BstFNI CGCG 1 cut(s) 1346
BstH2I RGCGCY 1 cut(s) 1441
BstHHI GCGC 3 cut(s) 198, 1348, 1440
BstMAI GTCTC 3 cut(s) 194, 222, 740
BstMWI GCNNNNNNNGC 3 cut(s) 598, 884, 1441
BstNI CCWGG 1 cut(s) 1544
BstNSI RCATGY 1 cut(s) 217
BstPI GGTNACC 1 cut(s) 998
BstSCI CCNGG 1 cut(s) 1542
BstSFI CTRYAG 1 cut(s) 108
BstUI CGCG 1 cut(s) 1346
BstV1I GCAGC 2 cut(s) 280, 1447
BstXI CCANNNNNNTGG 1 cut(s) 469
Bsu36I CCTNAGG 1 cut(s) 174
BsuRI GGCC 3 cut(s) 310, 1087, 1542
BtgZI GCGATG 1 cut(s) 1299
BtsCI GGATG 5 cut(s) 384, 878, 1094, 1144, 1163
BtsIMutI CAGTG 5 cut(s) 129, 375, 570, 738, 1125
Cac8I GCNNGC 7 cut(s) 75, 91, 200, 649, 876, 1360, 1442
CaiI CAGNNNCTG 2 cut(s) 674, 1441
CfoI GCGC 3 cut(s) 198, 1348, 1440
Cfr10I RCCGGY 1 cut(s) 647
Cfr13I GGNCC 2 cut(s) 381, 1085
CseI GACGC 2 cut(s) 464, 1352
Csp6I GTAC 2 cut(s) 98, 264
CviAII CATG 8 cut(s) 9, 22, 214, 449, 593, 802, 938, 1135
CviQI GTAC 2 cut(s) 98, 264
DdeI CTNAG 7 cut(s) 174, 326, 579, 841, 1479, 1496, 1596
EaeI YGGCCR 1 cut(s) 1540
Eam1104I CTCTTC 4 cut(s) 46, 630, 1136, 1503
EarI CTCTTC 4 cut(s) 46, 630, 1136, 1503
Ecl136II GAGCTC 2 cut(s) 6, 1574
Eco130I CCWWGG 2 cut(s) 1102, 1414
Eco24I GRGCYC 3 cut(s) 8, 603, 1576
Eco31I GGTCTC 1 cut(s) 740
Eco47I GGWCC 1 cut(s) 381
Eco53kI GAGCTC 2 cut(s) 6, 1574
Eco57I CTGAAG 1 cut(s) 1101
Eco81I CCTNAGG 1 cut(s) 174
Eco91I GGTNACC 1 cut(s) 998
EcoICRI GAGCTC 2 cut(s) 6, 1574
EcoNI CCTNNNNNAGG 1 cut(s) 108
EcoO65I GGTNACC 1 cut(s) 998
EcoRI GAATTC 1 cut(s) 1460
EcoRII CCWGG 1 cut(s) 1542
EcoT14I CCWWGG 2 cut(s) 1102, 1414
EcoT22I ATGCAT 2 cut(s) 27, 1163
EcoT38I GRGCYC 3 cut(s) 8, 603, 1576
ErhI CCWWGG 2 cut(s) 1102, 1414
FaeI CATG 8 cut(s) 12, 25, 217, 452, 596, 805, 941, 1138
FaqI GGGAC 2 cut(s) 250, 394
FatI CATG 8 cut(s) 8, 21, 213, 448, 592, 801, 937, 1134
Fnu4HI GCNGC 3 cut(s) 269, 846, 1436
FokI GGATG 5 cut(s) 371, 865, 1081, 1151, 1170
FriOI GRGCYC 3 cut(s) 8, 603, 1576
Fsp4HI GCNGC 3 cut(s) 269, 846, 1436
FspBI CTAG 8 cut(s) 48, 702, 968, 1103, 1212, 1239, 1323, 1371
GlaI GCGC 3 cut(s) 197, 1347, 1439
GluI GCNGC 3 cut(s) 269, 846, 1436
GsaI CCCAGC 2 cut(s) 473, 904
GsuI CTGGAG 1 cut(s) 656
HaeII RGCGCY 1 cut(s) 1441
HaeIII GGCC 3 cut(s) 310, 1087, 1542
HapII CCGG 1 cut(s) 648
HgaI GACGC 2 cut(s) 464, 1352
HhaI GCGC 3 cut(s) 198, 1348, 1440
Hin1I GRCGYC 1 cut(s) 475
Hin1II CATG 8 cut(s) 12, 25, 217, 452, 596, 805, 941, 1138
Hin6I GCGC 3 cut(s) 196, 1346, 1438
HinP1I GCGC 3 cut(s) 196, 1346, 1438
HindIII AAGCTT 1 cut(s) 1037
HinfI GANTC 9 cut(s) 17, 54, 115, 135, 495, 529, 583, 712, 1066
HpaII CCGG 1 cut(s) 648
HphI GGTGA 2 cut(s) 446, 1409
Hpy166II GTNNAC 4 cut(s) 746, 793, 973, 1307
Hpy188I TCNGA 6 cut(s) 246, 366, 582, 711, 1071, 1599
Hpy8I GTNNAC 4 cut(s) 746, 793, 973, 1307
Hpy99I CGWCG 1 cut(s) 1346
HpyAV CCTTC 8 cut(s) 114, 238, 528, 543, 762, 989, 1046, 1445
HpyCH4III ACNGT 4 cut(s) 349, 574, 676, 1282
HpyCH4IV ACGT 1 cut(s) 391
HpyF10VI GCNNNNNNNGC 3 cut(s) 598, 884, 1441
HpyF3I CTNAG 7 cut(s) 174, 326, 579, 841, 1479, 1496, 1596
HpySE526I ACGT 1 cut(s) 391
Hsp92I GRCGYC 1 cut(s) 475
Hsp92II CATG 8 cut(s) 12, 25, 217, 452, 596, 805, 941, 1138
HspAI GCGC 3 cut(s) 196, 1346, 1438
KroI GCCGGC 1 cut(s) 647
KroNI GCCGGC 1 cut(s) 649
LguI GCTCTTC 1 cut(s) 1136
LmnI GCTCC 2 cut(s) 98, 598
Lsp1109I GCAGC 2 cut(s) 280, 1447
LweI GCATC 5 cut(s) 692, 887, 1015, 1129, 1148
MaeI CTAG 8 cut(s) 48, 702, 968, 1103, 1212, 1239, 1323, 1371
MaeII ACGT 1 cut(s) 391
MaeIII GTNAC 5 cut(s) 58, 574, 998, 1120, 1214
MboII GAAGA 6 cut(s) 63, 402, 528, 617, 1123, 1520
MhlI GDGCHC 3 cut(s) 8, 603, 1576
MlsI TGGCCA 1 cut(s) 1542
MluNI TGGCCA 1 cut(s) 1542
MlyI GAGTC 1 cut(s) 63
MmeI TCCRAC 5 cut(s) 17, 344, 374, 503, 1494
Mox20I TGGCCA 1 cut(s) 1542
Mph1103I ATGCAT 2 cut(s) 27, 1163
MroNI GCCGGC 1 cut(s) 647
MscI TGGCCA 1 cut(s) 1542
MseI TTAA 3 cut(s) 344, 782, 1257
Msp20I TGGCCA 1 cut(s) 1542
MspA1I CMGCKG 2 cut(s) 271, 1467
MspI CCGG 1 cut(s) 648
MspR9I CCNGG 1 cut(s) 1544
MvaI CCWGG 1 cut(s) 1544
MvnI CGCG 1 cut(s) 1346
MwoI GCNNNNNNNGC 3 cut(s) 598, 884, 1441
NaeI GCCGGC 1 cut(s) 649
NgoMIV GCCGGC 1 cut(s) 647
NlaIII CATG 8 cut(s) 12, 25, 217, 452, 596, 805, 941, 1138
NlaIV GGNNCC 4 cut(s) 94, 382, 600, 1548
NmuCI GTSAC 2 cut(s) 58, 1120
NsiI ATGCAT 2 cut(s) 27, 1163
NspI RCATGY 1 cut(s) 217
PciI ACATGT 1 cut(s) 213
PciSI GCTCTTC 1 cut(s) 1136
PdiI GCCGGC 1 cut(s) 649
PfeI GAWTC 8 cut(s) 17, 115, 135, 495, 529, 583, 712, 1066
PflFI GACNNNGTC 1 cut(s) 1282
PflMI CCANNNNNTGG 1 cut(s) 1175
PkrI GCNGC 3 cut(s) 270, 847, 1437
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
PscI ACATGT 1 cut(s) 213
Psp124BI GAGCTC 2 cut(s) 8, 1576
Psp6I CCWGG 1 cut(s) 1542
PspEI GGTNACC 1 cut(s) 998
PspFI CCCAGC 2 cut(s) 469, 900
PspGI CCWGG 1 cut(s) 1542
PspN4I GGNNCC 4 cut(s) 94, 382, 600, 1548
PspPI GGNCC 2 cut(s) 381, 1085
PstNI CAGNNNCTG 2 cut(s) 674, 1441
PsyI GACNNNGTC 1 cut(s) 1282
PvuII CAGCTG 1 cut(s) 271
RsaI GTAC 2 cut(s) 99, 265
RsaNI GTAC 2 cut(s) 98, 264
SacI GAGCTC 2 cut(s) 8, 1576
SapI GCTCTTC 1 cut(s) 1136
SaqAI TTAA 3 cut(s) 344, 782, 1257
SatI GCNGC 3 cut(s) 269, 846, 1436
Sau96I GGNCC 2 cut(s) 381, 1085
SchI GAGTC 1 cut(s) 63
ScrFI CCNGG 1 cut(s) 1544
SduI GDGCHC 3 cut(s) 8, 603, 1576
SfaNI GCATC 5 cut(s) 692, 887, 1015, 1129, 1148
SfcI CTRYAG 1 cut(s) 108
SinI GGWCC 1 cut(s) 381
SmlI CTYRAG 2 cut(s) 826, 1577
SmoI CTYRAG 2 cut(s) 826, 1577
SsiI CCGC 3 cut(s) 651, 846, 1467
SspMI CTAG 8 cut(s) 48, 702, 968, 1103, 1212, 1239, 1323, 1371
SstI GAGCTC 2 cut(s) 8, 1576
StyD4I CCNGG 1 cut(s) 1542
StyI CCWWGG 2 cut(s) 1102, 1414
TaaI ACNGT 4 cut(s) 349, 574, 676, 1282
TaiI ACGT 1 cut(s) 394
TaqI TCGA 3 cut(s) 539, 1268, 1570
TauI GCSGC 1 cut(s) 848
TfiI GAWTC 8 cut(s) 17, 115, 135, 495, 529, 583, 712, 1066
Tru1I TTAA 3 cut(s) 344, 782, 1257
Tru9I TTAA 3 cut(s) 344, 782, 1257
TscAI CASTG 5 cut(s) 136, 382, 577, 745, 1125
TseFI GTSAC 2 cut(s) 58, 1120
TseI GCWGC 2 cut(s) 268, 1435
Tsp45I GTSAC 2 cut(s) 58, 1120
TspDTI ATGAA 5 cut(s) 30, 487, 818, 1123, 1473
TspGWI ACGGA 1 cut(s) 85
TspRI CASTG 5 cut(s) 136, 382, 577, 745, 1125
Tth111I GACNNNGTC 1 cut(s) 1282
Van91I CCANNNNNTGG 1 cut(s) 1175
VpaK11BI GGWCC 1 cut(s) 381
XagI CCTNNNNNAGG 1 cut(s) 108
XapI RAATTY 5 cut(s) 662, 909, 928, 1460, 1553
XbaI TCTAGA 2 cut(s) 701, 1370
XceI RCATGY 1 cut(s) 217
XmaJI CCTAGG 1 cut(s) 1102
XspI CTAG 8 cut(s) 48, 702, 968, 1103, 1212, 1239, 1323, 1371
Zsp2I ATGCAT 2 cut(s) 27, 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.