RchiOBHm_Chr3g0471031

2-aminoethanethiol dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0017877
Physical Location & Seq
Forward (+)
16962397 .. 16967284
4888 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43678

Sequence Viewer

Length: 414 bp
ATGAACGAAGAGGTTGCGATTAAGCTGGAATATGCAATCCATCCTGCTGATGTTGGACTTAAAGAGGAAAATCCAGATGATGATCGAGGGCTTGGTTTTTTCAGAGACAATCTGAATAGAGTTTCTCGACGGACTCAACCAATAACATACTTCAATTTATATGAAGGTGATAGTTTTACGATGTGTATATTCTGTTTTCCTACTTCCTCAGTCATTCCACTTCATGATCACCCTGGCATGACTGTTTTTAGCAAAGTTCTGTATGGTTCTTTGCATGTGAGAGCTTACAATTGGGTGGAGCCTTCCCACGAAAGCAAGGGATCAAATTACTTTCCAGGAGATGAGATGACTCGAATTTTCTGGAAATCAATCAAGGATAAGCTTATTCTCCCCTTTTTGGATTTGGACATTTAA

Protein Analysis

137

Amino Acids

15.86

Weight (kDa)

5.27

Isoelectric Point (pI)

27.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 12 - 108 1.7e-32 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017877)

Species Orthologous Gene IDs
malus_domestica MD10G1219400.v1.1
pyrus_communis pycom10g18550
rosa_chinensis RchiOBHm_Chr3g0471031 RchiOBHm_Chr3g0489021 RchiOBHm_Chr3g0492451
rosa_multiflora Rmu_sc0019359.1_g000001
rosa_roxburghii Rroxscaffold_3G00234280
rosa_rugosa Rorug05G0306800
rosa_samantha Rh3BG190100 Rh3CG180000
rosa_wichuraiana Rw1G029100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 328
AcsI RAATTY 1 cut(s) 354
AfiI CCNNNNNNNGG 1 cut(s) 397
AgsI TTSAA 1 cut(s) 154
AjnI CCWGG 2 cut(s) 232, 334
AluBI AGCT 3 cut(s) 25, 284, 382
AluI AGCT 3 cut(s) 25, 284, 382
Alw26I GTCTC 1 cut(s) 99
AlwI GGATC 1 cut(s) 328
ApoI RAATTY 1 cut(s) 354
AsuHPI GGTGA 2 cut(s) 179, 221
BccI CCATC 1 cut(s) 48
BcgI CGANNNNNNTGC 1 cut(s) 30
BciT130I CCWGG 2 cut(s) 234, 336
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 1 cut(s) 99
Bme1390I CCNGG 2 cut(s) 234, 336
BmiI GGNNCC 1 cut(s) 300
BmrFI CCNGG 2 cut(s) 234, 336
BsaBI GATNNNNATC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 232
Bsc4I CCNNNNNNNGG 1 cut(s) 397
Bse8I GATNNNNATC 1 cut(s) 81
BseBI CCWGG 2 cut(s) 234, 336
BseDI CCNNGG 1 cut(s) 232
BseGI GGATG 1 cut(s) 40
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 397
BseMII CTCAG 1 cut(s) 222
BslI CCNNNNNNNGG 1 cut(s) 397
BsmAI GTCTC 1 cut(s) 99
Bsp143I GATC 3 cut(s) 82, 226, 320
BspCNI CTCAG 1 cut(s) 221
BspHI TCATGA 1 cut(s) 223
BspLI GGNNCC 1 cut(s) 300
BspPI GGATC 1 cut(s) 328
BssECI CCNNGG 1 cut(s) 232
BssMI GATC 3 cut(s) 82, 226, 320
Bst2UI CCWGG 2 cut(s) 234, 336
Bst4CI ACNGT 1 cut(s) 244
Bst6I CTCTTC 1 cut(s) 3
BstDEI CTNAG 1 cut(s) 208
BstF5I GGATG 1 cut(s) 40
BstKTI GATC 3 cut(s) 85, 229, 323
BstMAI GTCTC 1 cut(s) 99
BstMBI GATC 3 cut(s) 82, 226, 320
BstNI CCWGG 2 cut(s) 234, 336
BstNSI RCATGY 1 cut(s) 278
BstSCI CCNGG 2 cut(s) 232, 334
BtsCI GGATG 1 cut(s) 40
CciI TCATGA 1 cut(s) 223
CviAII CATG 3 cut(s) 224, 238, 275
CviJI RGCY 5 cut(s) 25, 91, 284, 301, 382
CviKI_1 RGCY 5 cut(s) 25, 91, 284, 301, 382
DdeI CTNAG 1 cut(s) 208
DpnI GATC 3 cut(s) 84, 228, 322
DpnII GATC 3 cut(s) 82, 226, 320
Eam1104I CTCTTC 1 cut(s) 3
EarI CTCTTC 1 cut(s) 3
EcoRII CCWGG 2 cut(s) 232, 334
FaeI CATG 3 cut(s) 227, 241, 278
FaiI YATR 9 cut(s) 33, 148, 160, 162, 188, 225, 239, 264, 276
FatI CATG 3 cut(s) 223, 237, 274
FbaI TGATCA 1 cut(s) 226
FokI GGATG 1 cut(s) 27
Hin1II CATG 3 cut(s) 227, 241, 278
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 2 cut(s) 133, 349
HphI GGTGA 2 cut(s) 179, 221
Hpy188I TCNGA 2 cut(s) 104, 114
Hpy188III TCNNGA 4 cut(s) 74, 126, 224, 361
Hpy99I CGWCG 1 cut(s) 132
HpyAV CCTTC 2 cut(s) 158, 312
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4V TGCA 2 cut(s) 35, 274
HpyF3I CTNAG 1 cut(s) 208
Hsp92II CATG 3 cut(s) 227, 241, 278
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 3 cut(s) 82, 226, 320
LmnI GCTCC 1 cut(s) 298
LpnPI CCDG 8 cut(s) 11, 57, 87, 219, 246, 321, 346, 348
MalI GATC 3 cut(s) 84, 228, 322
MboI GATC 3 cut(s) 82, 226, 320
MboII GAAGA 1 cut(s) 20
MfeI CAATTG 1 cut(s) 289
MluCI AATT 4 cut(s) 154, 289, 325, 354
MlyI GAGTC 2 cut(s) 127, 343
MmeI TCCRAC 1 cut(s) 34
MnlI CCTC 4 cut(s) 4, 58, 80, 217
MseI TTAA 3 cut(s) 21, 60, 412
MspR9I CCNGG 2 cut(s) 234, 336
MunI CAATTG 1 cut(s) 289
MvaI CCWGG 2 cut(s) 234, 336
NdeII GATC 3 cut(s) 82, 226, 320
NlaIII CATG 3 cut(s) 227, 241, 278
NlaIV GGNNCC 1 cut(s) 300
NspI RCATGY 1 cut(s) 278
PagI TCATGA 1 cut(s) 223
PfoI TCCNGGA 1 cut(s) 334
PleI GAGTC 2 cut(s) 127, 343
PpsI GAGTC 2 cut(s) 127, 343
Psp6I CCWGG 2 cut(s) 232, 334
PspGI CCWGG 2 cut(s) 232, 334
PspN4I GGNNCC 1 cut(s) 300
SaqAI TTAA 3 cut(s) 21, 60, 412
Sau3AI GATC 3 cut(s) 82, 226, 320
SchI GAGTC 2 cut(s) 127, 343
ScrFI CCNGG 2 cut(s) 234, 336
SetI ASST 5 cut(s) 15, 27, 169, 286, 384
Sse9I AATT 4 cut(s) 154, 289, 325, 354
StyD4I CCNGG 2 cut(s) 232, 334
TaaI ACNGT 1 cut(s) 244
TaqI TCGA 3 cut(s) 85, 127, 352
TasI AATT 4 cut(s) 154, 289, 325, 354
Tru1I TTAA 3 cut(s) 21, 60, 412
Tru9I TTAA 3 cut(s) 21, 60, 412
TspDTI ATGAA 3 cut(s) 17, 177, 212
TspGWI ACGGA 1 cut(s) 145
XapI RAATTY 1 cut(s) 354
XceI RCATGY 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.