Rh3BG190100

2-aminoethanethiol dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0017877
Physical Location & Seq
Forward (+)
16754659 .. 16768790
14132 bp
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UTR
Exon/CDS
Intron
Rh3BG190100.1

Sequence Viewer

Length: 438 bp
ATGGTCGTCTCCATCCGTATACCTGAAAACGAGTCCCAGTCAGATTCTCATTGGCCTCAAATTCATGGGTTCGTAAAGCAATGTACCAATATTCAGATGAACGAAGAGGTTGCGATTAAGCTGATGCAATCCATCCTGCTGATAGTTTCTCGACGGACTCAACCAATAACATACTTCAATTTATATGAAGGTGATAGTTTTACGATGTGTATATTCTGTTTTCCTACTTCCTCAGTCATTCCACTTCATGATCACCCTGGCATGACTGTTTTTAGCAAAGTTCTGTATGGTTCTTTGCATGTGAGAGCTTACAATTGGGTGGAGCCTTCCCACGAAAGCAAGGGATCAAATTACTTTCCAGGAGATGAGATGACTCGAATTTTCTGGAAATCAATCAAGGATAAGCTTATTCTCCCCTTTTTGGATTTGGACATTTAA

Protein Analysis

145

Amino Acids

16.8

Weight (kDa)

6.03

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 50 - 116 3.7e-29 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017877)

Species Orthologous Gene IDs
malus_domestica MD10G1219400.v1.1
pyrus_communis pycom10g18550
rosa_chinensis RchiOBHm_Chr3g0471031 RchiOBHm_Chr3g0489021 RchiOBHm_Chr3g0492451
rosa_multiflora Rmu_sc0019359.1_g000001
rosa_roxburghii Rroxscaffold_3G00234280
rosa_rugosa Rorug05G0306800
rosa_samantha Rh3BG190100 Rh3CG180000
rosa_wichuraiana Rw1G029100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 19
AclWI GGATC 1 cut(s) 352
AcsI RAATTY 2 cut(s) 60, 378
AfaI GTAC 1 cut(s) 85
AfiI CCNNNNNNNGG 1 cut(s) 421
AgsI TTSAA 1 cut(s) 178
AjnI CCWGG 2 cut(s) 256, 358
AluBI AGCT 3 cut(s) 121, 308, 406
AluI AGCT 3 cut(s) 121, 308, 406
Alw26I GTCTC 1 cut(s) 13
AlwI GGATC 1 cut(s) 352
AoxI GGCC 1 cut(s) 53
ApoI RAATTY 2 cut(s) 60, 378
AsuHPI GGTGA 2 cut(s) 203, 245
BccI CCATC 2 cut(s) 20, 140
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BciT130I CCWGG 2 cut(s) 258, 360
BclI TGATCA 1 cut(s) 250
BcoDI GTCTC 1 cut(s) 13
Bme1390I CCNGG 2 cut(s) 258, 360
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 2 cut(s) 258, 360
BmrI ACTGGG 1 cut(s) 31
BmsI GCATC 1 cut(s) 114
BmuI ACTGGG 1 cut(s) 31
BsaJI CCNNGG 1 cut(s) 256
Bsc4I CCNNNNNNNGG 1 cut(s) 421
Bse1I ACTGG 1 cut(s) 37
Bse3DI GCAATG 1 cut(s) 86
BseBI CCWGG 2 cut(s) 258, 360
BseDI CCNNGG 1 cut(s) 256
BseGI GGATG 2 cut(s) 12, 132
BseLI CCNNNNNNNGG 1 cut(s) 421
BseMI GCAATG 1 cut(s) 86
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 37
BshFI GGCC 1 cut(s) 55
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 1 cut(s) 421
BsmAI GTCTC 1 cut(s) 13
BsmBI CGTCTC 1 cut(s) 13
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 1 cut(s) 55
Bsp143I GATC 2 cut(s) 250, 344
BspANI GGCC 1 cut(s) 55
BspCNI CTCAG 1 cut(s) 245
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 1 cut(s) 324
BspPI GGATC 1 cut(s) 352
BsrDI GCAATG 1 cut(s) 86
BsrI ACTGG 1 cut(s) 37
BssECI CCNNGG 1 cut(s) 256
BssMI GATC 2 cut(s) 250, 344
BssNAI GTATAC 1 cut(s) 20
Bst1107I GTATAC 1 cut(s) 20
Bst2UI CCWGG 2 cut(s) 258, 360
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 99
BstDEI CTNAG 1 cut(s) 232
BstF5I GGATG 2 cut(s) 12, 132
BstKTI GATC 2 cut(s) 253, 347
BstMAI GTCTC 1 cut(s) 13
BstMBI GATC 2 cut(s) 250, 344
BstNI CCWGG 2 cut(s) 258, 360
BstNSI RCATGY 1 cut(s) 302
BstSCI CCNGG 2 cut(s) 256, 358
BstZ17I GTATAC 1 cut(s) 20
BsuRI GGCC 1 cut(s) 55
BtsCI GGATG 2 cut(s) 12, 132
CciI TCATGA 1 cut(s) 247
Csp6I GTAC 1 cut(s) 84
CviAII CATG 4 cut(s) 65, 248, 262, 299
CviJI RGCY 5 cut(s) 55, 121, 308, 325, 406
CviKI_1 RGCY 5 cut(s) 55, 121, 308, 325, 406
CviQI GTAC 1 cut(s) 84
DdeI CTNAG 1 cut(s) 232
DpnI GATC 2 cut(s) 252, 346
DpnII GATC 2 cut(s) 250, 344
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
EcoRII CCWGG 2 cut(s) 256, 358
Esp3I CGTCTC 1 cut(s) 13
FaeI CATG 4 cut(s) 68, 251, 265, 302
FaqI GGGAC 1 cut(s) 19
FatI CATG 4 cut(s) 64, 247, 261, 298
FbaI TGATCA 1 cut(s) 250
FblI GTMKAC 1 cut(s) 19
FokI GGATG 1 cut(s) 119
HaeIII GGCC 1 cut(s) 55
Hin1II CATG 4 cut(s) 68, 251, 265, 302
HindIII AAGCTT 1 cut(s) 404
HinfI GANTC 4 cut(s) 32, 44, 157, 373
HphI GGTGA 2 cut(s) 203, 245
Hpy166II GTNNAC 1 cut(s) 20
Hpy188I TCNGA 2 cut(s) 43, 96
Hpy188III TCNNGA 3 cut(s) 150, 248, 385
Hpy8I GTNNAC 1 cut(s) 20
Hpy99I CGWCG 1 cut(s) 156
HpyAV CCTTC 2 cut(s) 182, 336
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4V TGCA 2 cut(s) 127, 298
HpyF3I CTNAG 1 cut(s) 232
Hsp92II CATG 4 cut(s) 68, 251, 265, 302
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 2 cut(s) 250, 344
LmnI GCTCC 1 cut(s) 322
LpnPI CCDG 8 cut(s) 36, 50, 149, 243, 270, 345, 370, 372
LweI GCATC 1 cut(s) 114
MalI GATC 2 cut(s) 252, 346
MboI GATC 2 cut(s) 250, 344
MboII GAAGA 1 cut(s) 116
MfeI CAATTG 1 cut(s) 313
MluCI AATT 5 cut(s) 60, 178, 313, 349, 378
MlyI GAGTC 3 cut(s) 41, 151, 367
MnlI CCTC 3 cut(s) 66, 100, 241
MseI TTAA 2 cut(s) 117, 436
MspR9I CCNGG 2 cut(s) 258, 360
MunI CAATTG 1 cut(s) 313
MvaI CCWGG 2 cut(s) 258, 360
NdeII GATC 2 cut(s) 250, 344
NlaIII CATG 4 cut(s) 68, 251, 265, 302
NlaIV GGNNCC 1 cut(s) 324
NspI RCATGY 1 cut(s) 302
PagI TCATGA 1 cut(s) 247
PfeI GAWTC 1 cut(s) 44
PfoI TCCNGGA 1 cut(s) 358
PleI GAGTC 3 cut(s) 40, 151, 367
PpsI GAGTC 3 cut(s) 40, 151, 367
Psp6I CCWGG 2 cut(s) 256, 358
PspGI CCWGG 2 cut(s) 256, 358
PspN4I GGNNCC 1 cut(s) 324
RsaI GTAC 1 cut(s) 85
RsaNI GTAC 1 cut(s) 84
SaqAI TTAA 2 cut(s) 117, 436
Sau3AI GATC 2 cut(s) 250, 344
SchI GAGTC 3 cut(s) 41, 151, 367
ScrFI CCNGG 2 cut(s) 258, 360
SetI ASST 6 cut(s) 25, 111, 123, 193, 310, 408
SfaNI GCATC 1 cut(s) 114
Sse9I AATT 5 cut(s) 60, 178, 313, 349, 378
SspI AATATT 1 cut(s) 91
StyD4I CCNGG 2 cut(s) 256, 358
TaaI ACNGT 1 cut(s) 268
TaqI TCGA 2 cut(s) 151, 376
TasI AATT 5 cut(s) 60, 178, 313, 349, 378
TfiI GAWTC 1 cut(s) 44
Tru1I TTAA 2 cut(s) 117, 436
Tru9I TTAA 2 cut(s) 117, 436
TspDTI ATGAA 4 cut(s) 53, 113, 201, 236
TspGWI ACGGA 2 cut(s) 5, 169
XapI RAATTY 2 cut(s) 60, 378
XceI RCATGY 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.