Rh3CG180000

2-aminoethanethiol dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0017877
Physical Location & Seq
Forward (+)
15808612 .. 15820107
11496 bp
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UTR
Exon/CDS
Intron
Rh3CG180000.1

Sequence Viewer

Length: 483 bp
ATGGCATCCCAAACCCCCCAGCTTCACTTTACCTTATTCCCTCTTATGGCCCAAGGCCACATGATTCCAATGGTTGATATGGCTAGAATGTTGGCACTGCGAGCCCCTTTCCTTTTTCTCCTCCTTTGTACCAATATTCAGATGAACGAAGAGGTTGCGATTAAGCTGATGCAATCCATCCTGCTGATAGTTTCTCGACGGACTCAACCAATAACATACTTCAATTTATATGAAGGTGATAGTTTTACGATGTGTATATTCTGTTTTCCTACTTCCTCAGTCATTCCACTTCATGATCACCCTGGCATGACTGTTTTTAGCAAAGTTCTGTATGGTTCTTTGCATGTGAGAGCTTACAATTGGGTGGAGCCTTCCCACGAAAGCAAGGGATCAAATTACTTTCCAGGAGATGAGATGACTCGAATTTTCTGGAAATCAATCAAGGATAAGCTTATTCTCCCCTTTTTGGATTTGGACATTTAA

Protein Analysis

160

Amino Acids

18.45

Weight (kDa)

6.57

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 65 - 131 5.3e-29 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017877)

Species Orthologous Gene IDs
malus_domestica MD10G1219400.v1.1
pyrus_communis pycom10g18550
rosa_chinensis RchiOBHm_Chr3g0471031 RchiOBHm_Chr3g0489021 RchiOBHm_Chr3g0492451
rosa_multiflora Rmu_sc0019359.1_g000001
rosa_roxburghii Rroxscaffold_3G00234280
rosa_rugosa Rorug05G0306800
rosa_samantha Rh3BG190100 Rh3CG180000
rosa_wichuraiana Rw1G029100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 397
AcsI RAATTY 1 cut(s) 423
AfaI GTAC 1 cut(s) 130
AfiI CCNNNNNNNGG 2 cut(s) 46, 466
AgsI TTSAA 1 cut(s) 223
AjnI CCWGG 2 cut(s) 301, 403
AluBI AGCT 4 cut(s) 22, 166, 353, 451
AluI AGCT 4 cut(s) 22, 166, 353, 451
AlwI GGATC 1 cut(s) 397
AoxI GGCC 2 cut(s) 48, 55
ApoI RAATTY 1 cut(s) 423
AspS9I GGNCC 1 cut(s) 49
AsuHPI GGTGA 2 cut(s) 248, 290
BanII GRGCYC 1 cut(s) 106
BccI CCATC 1 cut(s) 185
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 2 cut(s) 303, 405
BclI TGATCA 1 cut(s) 295
BfaI CTAG 1 cut(s) 84
Bme1390I CCNGG 2 cut(s) 303, 405
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 1 cut(s) 369
BmrFI CCNGG 2 cut(s) 303, 405
BmsI GCATC 2 cut(s) 14, 159
BsaJI CCNNGG 2 cut(s) 52, 301
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 466
BseBI CCWGG 2 cut(s) 303, 405
BseDI CCNNGG 2 cut(s) 52, 301
BseGI GGATG 2 cut(s) 5, 177
BseLI CCNNNNNNNGG 2 cut(s) 46, 466
BseMII CTCAG 1 cut(s) 291
BseRI GAGGAG 1 cut(s) 110
BseYI CCCAGC 1 cut(s) 18
BshFI GGCC 2 cut(s) 50, 57
BslI CCNNNNNNNGG 2 cut(s) 46, 466
BsnI GGCC 2 cut(s) 50, 57
Bsp1286I GDGCHC 1 cut(s) 106
Bsp143I GATC 2 cut(s) 295, 389
BspANI GGCC 2 cut(s) 50, 57
BspCNI CTCAG 1 cut(s) 290
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 369
BspPI GGATC 1 cut(s) 397
BssECI CCNNGG 2 cut(s) 52, 301
BssMI GATC 2 cut(s) 295, 389
BssT1I CCWWGG 1 cut(s) 52
Bst2UI CCWGG 2 cut(s) 303, 405
Bst4CI ACNGT 1 cut(s) 313
Bst6I CTCTTC 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 1 cut(s) 277
BstF5I GGATG 2 cut(s) 5, 177
BstKTI GATC 2 cut(s) 298, 392
BstMBI GATC 2 cut(s) 295, 389
BstMWI GCNNNNNNNGC 1 cut(s) 101
BstNI CCWGG 2 cut(s) 303, 405
BstNSI RCATGY 1 cut(s) 347
BstSCI CCNGG 2 cut(s) 301, 403
BsuRI GGCC 2 cut(s) 50, 57
BtsCI GGATG 2 cut(s) 5, 177
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 95
Cac8I GCNNGC 1 cut(s) 102
CciI TCATGA 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 49
Csp6I GTAC 1 cut(s) 129
CviAII CATG 4 cut(s) 61, 293, 307, 344
CviJI RGCY 9 cut(s) 22, 50, 57, 83, 104, 166, 353, 370, 451
CviKI_1 RGCY 9 cut(s) 22, 50, 57, 83, 104, 166, 353, 370, 451
CviQI GTAC 1 cut(s) 129
DdeI CTNAG 1 cut(s) 277
DpnI GATC 2 cut(s) 297, 391
DpnII GATC 2 cut(s) 295, 389
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
Eco130I CCWWGG 1 cut(s) 52
Eco24I GRGCYC 1 cut(s) 106
EcoRII CCWGG 2 cut(s) 301, 403
EcoT14I CCWWGG 1 cut(s) 52
EcoT38I GRGCYC 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 52
FaeI CATG 4 cut(s) 64, 296, 310, 347
FatI CATG 4 cut(s) 60, 292, 306, 343
FbaI TGATCA 1 cut(s) 295
FokI GGATG 1 cut(s) 164
FriOI GRGCYC 1 cut(s) 106
FspBI CTAG 1 cut(s) 84
GsaI CCCAGC 1 cut(s) 22
HaeIII GGCC 2 cut(s) 50, 57
Hin1II CATG 4 cut(s) 64, 296, 310, 347
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 3 cut(s) 64, 202, 418
HphI GGTGA 2 cut(s) 248, 290
Hpy188I TCNGA 1 cut(s) 141
Hpy188III TCNNGA 3 cut(s) 195, 293, 430
Hpy99I CGWCG 1 cut(s) 201
HpyAV CCTTC 2 cut(s) 227, 381
HpyCH4III ACNGT 1 cut(s) 313
HpyCH4V TGCA 2 cut(s) 172, 343
HpyF10VI GCNNNNNNNGC 1 cut(s) 101
HpyF3I CTNAG 1 cut(s) 277
Hsp92II CATG 4 cut(s) 64, 296, 310, 347
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 2 cut(s) 295, 389
LmnI GCTCC 1 cut(s) 367
LpnPI CCDG 7 cut(s) 32, 194, 288, 315, 390, 415, 417
LweI GCATC 2 cut(s) 14, 159
MaeI CTAG 1 cut(s) 84
MalI GATC 2 cut(s) 297, 391
MboI GATC 2 cut(s) 295, 389
MboII GAAGA 1 cut(s) 161
MfeI CAATTG 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 106
MluCI AATT 4 cut(s) 223, 358, 394, 423
MlyI GAGTC 2 cut(s) 196, 412
MnlI CCTC 4 cut(s) 51, 131, 145, 286
MseI TTAA 2 cut(s) 162, 481
MspR9I CCNGG 2 cut(s) 303, 405
MunI CAATTG 1 cut(s) 358
MvaI CCWGG 2 cut(s) 303, 405
MwoI GCNNNNNNNGC 1 cut(s) 101
NdeII GATC 2 cut(s) 295, 389
NlaIII CATG 4 cut(s) 64, 296, 310, 347
NlaIV GGNNCC 1 cut(s) 369
NspI RCATGY 1 cut(s) 347
PagI TCATGA 1 cut(s) 292
PfeI GAWTC 1 cut(s) 64
PfoI TCCNGGA 1 cut(s) 403
PleI GAGTC 2 cut(s) 196, 412
PpsI GAGTC 2 cut(s) 196, 412
Psp6I CCWGG 2 cut(s) 301, 403
PspFI CCCAGC 1 cut(s) 18
PspGI CCWGG 2 cut(s) 301, 403
PspN4I GGNNCC 1 cut(s) 369
PspPI GGNCC 1 cut(s) 49
RsaI GTAC 1 cut(s) 130
RsaNI GTAC 1 cut(s) 129
SaqAI TTAA 2 cut(s) 162, 481
Sau3AI GATC 2 cut(s) 295, 389
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 2 cut(s) 196, 412
ScrFI CCNGG 2 cut(s) 303, 405
SduI GDGCHC 1 cut(s) 106
SetI ASST 7 cut(s) 24, 35, 156, 168, 238, 355, 453
SfaNI GCATC 2 cut(s) 14, 159
Sse9I AATT 4 cut(s) 223, 358, 394, 423
SspI AATATT 1 cut(s) 136
SspMI CTAG 1 cut(s) 84
StyD4I CCNGG 2 cut(s) 301, 403
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 1 cut(s) 313
TaqI TCGA 2 cut(s) 196, 421
TasI AATT 4 cut(s) 223, 358, 394, 423
TfiI GAWTC 1 cut(s) 64
Tru1I TTAA 2 cut(s) 162, 481
Tru9I TTAA 2 cut(s) 162, 481
TscAI CASTG 1 cut(s) 102
TspDTI ATGAA 3 cut(s) 158, 246, 281
TspGWI ACGGA 1 cut(s) 214
TspRI CASTG 1 cut(s) 102
XapI RAATTY 1 cut(s) 423
XceI RCATGY 1 cut(s) 347
XspI CTAG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.