Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)
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| Species | Orthologous Gene IDs |
|---|---|
| rosa_chinensis | RchiOBHm_Chr3g0471611 |
| rosa_laevigata | RLG00000024160 RLG00000029662 |
| rosa_multiflora | Rmu_co8438809.1_g000001 Rmu_sc0039472.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00036120 |
| rosa_samantha | Rh3AG169400 Rh3BG195100 Rh3CG185800 Rh3DG179800 Rh4BG123500 Rh5DG379000 Rh6DG256900 Rh7BG292300 |
| rosa_wichuraiana | Rw0G008930 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1466 |
| AccB1I | GGYRCC | 1 cut(s) | 420 |
| AciI | CCGC | 8 cut(s) | 256, 557, 689, 829, 1292, 2253, 2525, 2651 |
| AclWI | GGATC | 6 cut(s) | 485, 498, 671, 687, 1106, 1793 |
| AcoI | YGGCCR | 1 cut(s) | 802 |
| AcsI | RAATTY | 5 cut(s) | 309, 820, 1016, 1067, 1386 |
| AcuI | CTGAAG | 3 cut(s) | 356, 527, 1525 |
| AdeI | CACNNNGTG | 1 cut(s) | 487 |
| AfaI | GTAC | 3 cut(s) | 88, 921, 1266 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 394, 430, 2058 |
| AflII | CTTAAG | 1 cut(s) | 1735 |
| AflIII | ACRYGT | 5 cut(s) | 809, 1615, 1622, 1639, 2601 |
| AhlI | ACTAGT | 1 cut(s) | 110 |
| AjiI | CACGTC | 1 cut(s) | 1623 |
| AleI | CACNNNNGTG | 1 cut(s) | 1737 |
| Alw21I | GWGCWC | 2 cut(s) | 133, 2674 |
| Alw26I | GTCTC | 3 cut(s) | 1736, 2189, 2337 |
| Alw44I | GTGCAC | 1 cut(s) | 2670 |
| AlwI | GGATC | 6 cut(s) | 485, 498, 671, 687, 1106, 1793 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 305 |
| Ama87I | CYCGRG | 3 cut(s) | 588, 1722, 2446 |
| AoxI | GGCC | 6 cut(s) | 339, 685, 772, 802, 1719, 2426 |
| ApaLI | GTGCAC | 1 cut(s) | 2670 |
| ApeKI | GCWGC | 6 cut(s) | 299, 1187, 1190, 1571, 1610, 2522 |
| ApoI | RAATTY | 5 cut(s) | 309, 820, 1016, 1067, 1386 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1238, 1270 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1386 |
| AspLEI | GCGC | 2 cut(s) | 481, 1467 |
| AspS9I | GGNCC | 5 cut(s) | 5, 686, 1001, 1720, 2488 |
| AsuC2I | CCSGG | 3 cut(s) | 1723, 1724, 2340 |
| AsuHPI | GGTGA | 7 cut(s) | 163, 711, 748, 1607, 2143, 2465, 2750 |
| AsuNHI | GCTAGC | 1 cut(s) | 2704 |
| AvaI | CYCGRG | 3 cut(s) | 588, 1722, 2446 |
| AvaII | GGWCC | 3 cut(s) | 5, 1001, 2488 |
| AxyI | CCTNAGG | 1 cut(s) | 9 |
| BaeGI | GKGCMC | 1 cut(s) | 2674 |
| BalI | TGGCCA | 1 cut(s) | 804 |
| BamHI | GGATCC | 1 cut(s) | 490 |
| BanI | GGYRCC | 1 cut(s) | 420 |
| BauI | CACGAG | 1 cut(s) | 482 |
| BbsI | GAAGAC | 4 cut(s) | 806, 1663, 1929, 2858 |
| Bbv12I | GWGCWC | 2 cut(s) | 133, 2674 |
| BbvI | GCAGC | 6 cut(s) | 311, 1174, 1202, 1583, 1622, 2509 |
| BccI | CCATC | 8 cut(s) | 605, 857, 1403, 1526, 1673, 2159, 2173, 2456 |
| BceAI | ACGGC | 2 cut(s) | 1881, 2376 |
| BcnI | CCSGG | 3 cut(s) | 1723, 1724, 2340 |
| BcoDI | GTCTC | 3 cut(s) | 1736, 2189, 2337 |
| BcuI | ACTAGT | 1 cut(s) | 110 |
| BfmI | CTRYAG | 2 cut(s) | 783, 1188 |
| BfrI | CTTAAG | 1 cut(s) | 1735 |
| BglII | AGATCT | 1 cut(s) | 2467 |
| BisI | GCNGC | 7 cut(s) | 300, 830, 1188, 1191, 1572, 1611, 2523 |
| BlpI | GCTNAGC | 1 cut(s) | 132 |
| BlsI | GCNGC | 7 cut(s) | 301, 831, 1189, 1192, 1573, 1612, 2524 |
| Bme1390I | CCNGG | 3 cut(s) | 1723, 1724, 2340 |
| Bme18I | GGWCC | 3 cut(s) | 5, 1001, 2488 |
| BmeT110I | CYCGRG | 3 cut(s) | 588, 1722, 2446 |
| BmgBI | CACGTC | 1 cut(s) | 1623 |
| BmgT120I | GGNCC | 5 cut(s) | 5, 686, 1001, 1720, 2488 |
| BmiI | GGNNCC | 6 cut(s) | 6, 422, 492, 1200, 2489, 2490 |
| BmrFI | CCNGG | 3 cut(s) | 1723, 1724, 2340 |
| BmrI | ACTGGG | 1 cut(s) | 2677 |
| BmtI | GCTAGC | 1 cut(s) | 2708 |
| BmuI | ACTGGG | 1 cut(s) | 2677 |
| BpiI | GAAGAC | 4 cut(s) | 806, 1663, 1929, 2858 |
| BpmI | CTGGAG | 1 cut(s) | 227 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1500 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 132 |
| BpuEI | CTTGAG | 2 cut(s) | 1420, 1900 |
| BpuMI | CCSGG | 3 cut(s) | 1723, 1724, 2340 |
| BsaAI | YACGTR | 1 cut(s) | 1271 |
| BsaBI | GATNNNNATC | 2 cut(s) | 2466, 2472 |
| BsaJI | CCNNGG | 4 cut(s) | 659, 1722, 2445, 2856 |
| BsaWI | WCCGGW | 1 cut(s) | 176 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1124, 1154 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 394, 430, 2058 |
| Bse118I | RCCGGY | 1 cut(s) | 1772 |
| Bse1I | ACTGG | 3 cut(s) | 210, 2230, 2683 |
| Bse21I | CCTNAGG | 1 cut(s) | 9 |
| Bse8I | GATNNNNATC | 2 cut(s) | 2466, 2472 |
| BseDI | CCNNGG | 4 cut(s) | 659, 1722, 2445, 2856 |
| BseGI | GGATG | 9 cut(s) | 19, 103, 1096, 1288, 1488, 1960, 2197, 2335, 2581 |
| BseJI | GATNNNNATC | 2 cut(s) | 2466, 2472 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 394, 430, 2058 |
| BseMII | CTCAG | 5 cut(s) | 146, 883, 1061, 1491, 1689 |
| BseNI | ACTGG | 3 cut(s) | 210, 2230, 2683 |
| BseRI | GAGGAG | 2 cut(s) | 1346, 1959 |
| BseSI | GKGCMC | 1 cut(s) | 2674 |
| BseXI | GCAGC | 6 cut(s) | 311, 1174, 1202, 1583, 1622, 2509 |
| BshFI | GGCC | 6 cut(s) | 341, 687, 774, 804, 1721, 2428 |
| BshNI | GGYRCC | 1 cut(s) | 420 |
| BsiHKAI | GWGCWC | 2 cut(s) | 133, 2674 |
| BsiHKCI | CYCGRG | 3 cut(s) | 588, 1722, 2446 |
| BsiSI | CCGG | 6 cut(s) | 177, 1559, 1723, 1773, 2052, 2339 |
| BslFI | GGGAC | 4 cut(s) | 18, 371, 2501, 2782 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 394, 430, 2058 |
| BsmAI | GTCTC | 3 cut(s) | 1736, 2189, 2337 |
| BsmFI | GGGAC | 4 cut(s) | 18, 371, 2501, 2782 |
| BsmI | GAATGC | 2 cut(s) | 1445, 2638 |
| BsnI | GGCC | 6 cut(s) | 341, 687, 774, 804, 1721, 2428 |
| BsoBI | CYCGRG | 3 cut(s) | 588, 1722, 2446 |
| Bsp1286I | GDGCHC | 2 cut(s) | 133, 2674 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 132 |
| Bsp19I | CCATGG | 1 cut(s) | 2856 |
| BspACI | CCGC | 8 cut(s) | 256, 557, 689, 829, 1292, 2253, 2525, 2651 |
| BspANI | GGCC | 6 cut(s) | 341, 687, 774, 804, 1721, 2428 |
| BspCNI | CTCAG | 5 cut(s) | 145, 882, 1060, 1492, 1690 |
| BspHI | TCATGA | 1 cut(s) | 565 |
| BspLI | GGNNCC | 6 cut(s) | 6, 422, 492, 1200, 2489, 2490 |
| BspMAI | CTGCAG | 1 cut(s) | 1192 |
| BspOI | GCTAGC | 1 cut(s) | 2708 |
| BspPI | GGATC | 6 cut(s) | 485, 498, 671, 687, 1106, 1793 |
| BspT107I | GGYRCC | 1 cut(s) | 420 |
| BspTI | CTTAAG | 1 cut(s) | 1735 |
| BsrFI | RCCGGY | 1 cut(s) | 1772 |
| BsrI | ACTGG | 3 cut(s) | 210, 2230, 2683 |
| BssAI | RCCGGY | 1 cut(s) | 1772 |
| BssECI | CCNNGG | 4 cut(s) | 659, 1722, 2445, 2856 |
| BssSI | CACGAG | 1 cut(s) | 482 |
| BssT1I | CCWWGG | 1 cut(s) | 2856 |
| Bst2BI | CACGAG | 1 cut(s) | 482 |
| Bst4CI | ACNGT | 9 cut(s) | 64, 148, 294, 329, 534, 1889, 1902, 1995, 2399 |
| Bst6I | CTCTTC | 1 cut(s) | 2164 |
| BstAFI | CTTAAG | 1 cut(s) | 1735 |
| BstBAI | YACGTR | 1 cut(s) | 1271 |
| BstC8I | GCNNGC | 2 cut(s) | 689, 2706 |
| BstDEI | CTNAG | 8 cut(s) | 9, 132, 429, 869, 1047, 1500, 1698, 2874 |
| BstDSI | CCRYGG | 1 cut(s) | 2856 |
| BstF5I | GGATG | 9 cut(s) | 19, 103, 1096, 1288, 1488, 1960, 2197, 2335, 2581 |
| BstHHI | GCGC | 2 cut(s) | 481, 1467 |
| BstMAI | GTCTC | 3 cut(s) | 1736, 2189, 2337 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 1057, 1568, 1927, 2705, 2714 |
| BstNSI | RCATGY | 6 cut(s) | 813, 1255, 1619, 1643, 1731, 2605 |
| BstSCI | CCNGG | 3 cut(s) | 1721, 1722, 2338 |
| BstSFI | CTRYAG | 2 cut(s) | 783, 1188 |
| BstSLI | GKGCMC | 1 cut(s) | 2674 |
| BstSNI | TACGTA | 1 cut(s) | 1271 |
| BstV1I | GCAGC | 6 cut(s) | 311, 1174, 1202, 1583, 1622, 2509 |
| BstV2I | GAAGAC | 4 cut(s) | 806, 1663, 1929, 2858 |
| BstX2I | RGATCY | 3 cut(s) | 490, 663, 2467 |
| BstYI | RGATCY | 3 cut(s) | 490, 663, 2467 |
| Bsu36I | CCTNAGG | 1 cut(s) | 9 |
| BsuRI | GGCC | 6 cut(s) | 341, 687, 774, 804, 1721, 2428 |
| BtgI | CCRYGG | 1 cut(s) | 2856 |
| BtrI | CACGTC | 1 cut(s) | 1623 |
| BtsCI | GGATG | 9 cut(s) | 19, 103, 1096, 1288, 1488, 1960, 2197, 2335, 2581 |
| BtsIMutI | CAGTG | 6 cut(s) | 153, 299, 325, 435, 2690, 2852 |
| Cac8I | GCNNGC | 2 cut(s) | 689, 2706 |
| CaiI | CAGNNNCTG | 1 cut(s) | 305 |
| CciI | TCATGA | 1 cut(s) | 565 |
| CfoI | GCGC | 2 cut(s) | 481, 1467 |
| Cfr10I | RCCGGY | 1 cut(s) | 1772 |
| Cfr13I | GGNCC | 5 cut(s) | 5, 686, 1001, 1720, 2488 |
| Cfr9I | CCCGGG | 1 cut(s) | 1722 |
| Csp6I | GTAC | 3 cut(s) | 87, 920, 1265 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 483, 518 |
| CviQI | GTAC | 3 cut(s) | 87, 920, 1265 |
| DdeI | CTNAG | 8 cut(s) | 9, 132, 429, 869, 1047, 1500, 1698, 2874 |
| DraI | TTTAAA | 2 cut(s) | 697, 2658 |
| DraIII | CACNNNGTG | 1 cut(s) | 487 |
| EaeI | YGGCCR | 1 cut(s) | 802 |
| Eam1104I | CTCTTC | 1 cut(s) | 2164 |
| EarI | CTCTTC | 1 cut(s) | 2164 |
| Eco105I | TACGTA | 1 cut(s) | 1271 |
| Eco130I | CCWWGG | 1 cut(s) | 2856 |
| Eco147I | AGGCCT | 1 cut(s) | 2428 |
| Eco32I | GATATC | 2 cut(s) | 1651, 1695 |
| Eco47I | GGWCC | 3 cut(s) | 5, 1001, 2488 |
| Eco57I | CTGAAG | 3 cut(s) | 356, 527, 1525 |
| Eco81I | CCTNAGG | 1 cut(s) | 9 |
| Eco88I | CYCGRG | 3 cut(s) | 588, 1722, 2446 |
| EcoO109I | RGGNCCY | 2 cut(s) | 5, 2488 |
| EcoRI | GAATTC | 1 cut(s) | 1386 |
| EcoRV | GATATC | 2 cut(s) | 1651, 1695 |
| EcoT14I | CCWWGG | 1 cut(s) | 2856 |
| EcoT22I | ATGCAT | 1 cut(s) | 395 |
| ErhI | CCWWGG | 1 cut(s) | 2856 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 510, 542, 1217, 1249, 1655, 1687 |
| FaqI | GGGAC | 4 cut(s) | 18, 371, 2501, 2782 |
| FauI | CCCGC | 2 cut(s) | 550, 696 |
| FauNDI | CATATG | 2 cut(s) | 949, 2388 |
| Fnu4HI | GCNGC | 7 cut(s) | 300, 830, 1188, 1191, 1572, 1611, 2523 |
| FokI | GGATG | 9 cut(s) | 26, 110, 1103, 1275, 1495, 1967, 2204, 2322, 2588 |
| Fsp4HI | GCNGC | 7 cut(s) | 300, 830, 1188, 1191, 1572, 1611, 2523 |
| FspI | TGCGCA | 1 cut(s) | 1466 |
| GlaI | GCGC | 2 cut(s) | 480, 1466 |
| GluI | GCNGC | 7 cut(s) | 300, 830, 1188, 1191, 1572, 1611, 2523 |
| GsuI | CTGGAG | 1 cut(s) | 227 |
| HaeIII | GGCC | 6 cut(s) | 341, 687, 774, 804, 1721, 2428 |
| HapII | CCGG | 6 cut(s) | 177, 1559, 1723, 1773, 2052, 2339 |
| HhaI | GCGC | 2 cut(s) | 481, 1467 |
| Hin6I | GCGC | 2 cut(s) | 479, 1465 |
| HinP1I | GCGC | 2 cut(s) | 479, 1465 |
| HincII | GTYRAC | 1 cut(s) | 1998 |
| HindII | GTYRAC | 1 cut(s) | 1998 |
| HindIII | AAGCTT | 3 cut(s) | 524, 539, 992 |
| HpaII | CCGG | 6 cut(s) | 177, 1559, 1723, 1773, 2052, 2339 |
| HphI | GGTGA | 7 cut(s) | 163, 711, 748, 1607, 2143, 2465, 2750 |
| Hpy166II | GTNNAC | 7 cut(s) | 217, 961, 1231, 1265, 1998, 2395, 2672 |
| Hpy8I | GTNNAC | 7 cut(s) | 217, 961, 1231, 1265, 1998, 2395, 2672 |
| HpyAV | CCTTC | 7 cut(s) | 331, 1322, 2028, 2122, 2269, 2293, 2660 |
| HpyCH4III | ACNGT | 9 cut(s) | 64, 148, 294, 329, 534, 1889, 1902, 1995, 2399 |
| HpyCH4IV | ACGT | 2 cut(s) | 1270, 1622 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 1057, 1568, 1927, 2705, 2714 |
| HpyF3I | CTNAG | 8 cut(s) | 9, 132, 429, 869, 1047, 1500, 1698, 2874 |
| HpySE526I | ACGT | 2 cut(s) | 1270, 1622 |
| HspAI | GCGC | 2 cut(s) | 479, 1465 |
| KflI | GGGWCCC | 1 cut(s) | 2488 |
| LmnI | GCTCC | 1 cut(s) | 458 |
| Lsp1109I | GCAGC | 6 cut(s) | 311, 1174, 1202, 1583, 1622, 2509 |
| MaeII | ACGT | 2 cut(s) | 1270, 1622 |
| MaeIII | GTNAC | 9 cut(s) | 323, 1150, 1213, 1304, 1618, 1730, 1775, 1854, 1883 |
| MfeI | CAATTG | 2 cut(s) | 1460, 2240 |
| MflI | RGATCY | 3 cut(s) | 490, 663, 2467 |
| MhlI | GDGCHC | 2 cut(s) | 133, 2674 |
| MlsI | TGGCCA | 1 cut(s) | 804 |
| MluNI | TGGCCA | 1 cut(s) | 804 |
| MlyI | GAGTC | 6 cut(s) | 861, 1294, 1607, 1738, 1891, 2255 |
| MmeI | TCCRAC | 1 cut(s) | 181 |
| Mox20I | TGGCCA | 1 cut(s) | 804 |
| Mph1103I | ATGCAT | 1 cut(s) | 395 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1386 |
| MscI | TGGCCA | 1 cut(s) | 804 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1737, 2294 |
| Msp20I | TGGCCA | 1 cut(s) | 804 |
| MspA1I | CMGCKG | 1 cut(s) | 1921 |
| MspCI | CTTAAG | 1 cut(s) | 1735 |
| MspI | CCGG | 6 cut(s) | 177, 1559, 1723, 1773, 2052, 2339 |
| MspR9I | CCNGG | 3 cut(s) | 1723, 1724, 2340 |
| MunI | CAATTG | 2 cut(s) | 1460, 2240 |
| Mva1269I | GAATGC | 2 cut(s) | 1445, 2638 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 1057, 1568, 1927, 2705, 2714 |
| NciI | CCSGG | 3 cut(s) | 1723, 1724, 2340 |
| NcoI | CCATGG | 1 cut(s) | 2856 |
| NdeI | CATATG | 2 cut(s) | 949, 2388 |
| NheI | GCTAGC | 1 cut(s) | 2704 |
| NlaIV | GGNNCC | 6 cut(s) | 6, 422, 492, 1200, 2489, 2490 |
| NmeAIII | GCCGAG | 2 cut(s) | 367, 1447 |
| NmuCI | GTSAC | 7 cut(s) | 323, 1150, 1213, 1618, 1730, 1854, 1883 |
| NsbI | TGCGCA | 1 cut(s) | 1466 |
| NsiI | ATGCAT | 1 cut(s) | 395 |
| NspI | RCATGY | 6 cut(s) | 813, 1255, 1619, 1643, 1731, 2605 |
| OliI | CACNNNNGTG | 1 cut(s) | 1737 |
| PaeR7I | CTCGAG | 1 cut(s) | 588 |
| PagI | TCATGA | 1 cut(s) | 565 |
| PceI | AGGCCT | 1 cut(s) | 2428 |
| PciI | ACATGT | 4 cut(s) | 809, 1615, 1639, 2601 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 586, 1267 |
| PctI | GAATGC | 2 cut(s) | 1445, 2638 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1386 |
| PflFI | GACNNNGTC | 1 cut(s) | 1747 |
| PfoI | TCCNGGA | 1 cut(s) | 2338 |
| PkrI | GCNGC | 7 cut(s) | 301, 831, 1189, 1192, 1573, 1612, 2524 |
| PleI | GAGTC | 6 cut(s) | 861, 1293, 1606, 1738, 1890, 2254 |
| PpsI | GAGTC | 6 cut(s) | 861, 1293, 1606, 1738, 1890, 2254 |
| Ppu21I | YACGTR | 1 cut(s) | 1271 |
| PpuMI | RGGWCCY | 2 cut(s) | 5, 2488 |
| PscI | ACATGT | 4 cut(s) | 809, 1615, 1639, 2601 |
| Psp5II | RGGWCCY | 2 cut(s) | 5, 2488 |
| PspN4I | GGNNCC | 6 cut(s) | 6, 422, 492, 1200, 2489, 2490 |
| PspPI | GGNCC | 5 cut(s) | 5, 686, 1001, 1720, 2488 |
| PspPPI | RGGWCCY | 2 cut(s) | 5, 2488 |
| PspXI | VCTCGAGB | 1 cut(s) | 588 |
| PstI | CTGCAG | 1 cut(s) | 1192 |
| PstNI | CAGNNNCTG | 1 cut(s) | 305 |
| PsuI | RGATCY | 3 cut(s) | 490, 663, 2467 |
| PsyI | GACNNNGTC | 1 cut(s) | 1747 |
| PvuII | CAGCTG | 1 cut(s) | 1921 |
| RsaI | GTAC | 3 cut(s) | 88, 921, 1266 |
| RsaNI | GTAC | 3 cut(s) | 87, 920, 1265 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1737, 2294 |
| SatI | GCNGC | 7 cut(s) | 300, 830, 1188, 1191, 1572, 1611, 2523 |
| Sau96I | GGNCC | 5 cut(s) | 5, 686, 1001, 1720, 2488 |
| SchI | GAGTC | 6 cut(s) | 861, 1294, 1607, 1738, 1891, 2255 |
| ScrFI | CCNGG | 3 cut(s) | 1723, 1724, 2340 |
| SduI | GDGCHC | 2 cut(s) | 133, 2674 |
| SfcI | CTRYAG | 2 cut(s) | 783, 1188 |
| Sfr274I | CTCGAG | 1 cut(s) | 588 |
| SinI | GGWCC | 3 cut(s) | 5, 1001, 2488 |
| SlaI | CTCGAG | 1 cut(s) | 588 |
| SmaI | CCCGGG | 1 cut(s) | 1724 |
| SmiI | ATTTAAAT | 1 cut(s) | 2658 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1737, 2294 |
| SmlI | CTYRAG | 4 cut(s) | 588, 1399, 1735, 1879 |
| SmoI | CTYRAG | 4 cut(s) | 588, 1399, 1735, 1879 |
| SnaBI | TACGTA | 1 cut(s) | 1271 |
| SpeI | ACTAGT | 1 cut(s) | 110 |
| SrfI | GCCCGGGC | 1 cut(s) | 1724 |
| SseBI | AGGCCT | 1 cut(s) | 2428 |
| SsiI | CCGC | 8 cut(s) | 256, 557, 689, 829, 1292, 2253, 2525, 2651 |
| SspI | AATATT | 2 cut(s) | 1240, 2588 |
| StuI | AGGCCT | 1 cut(s) | 2428 |
| StyD4I | CCNGG | 3 cut(s) | 1721, 1722, 2338 |
| StyI | CCWWGG | 1 cut(s) | 2856 |
| SwaI | ATTTAAAT | 1 cut(s) | 2658 |
| TaaI | ACNGT | 9 cut(s) | 64, 148, 294, 329, 534, 1889, 1902, 1995, 2399 |
| TaiI | ACGT | 2 cut(s) | 1273, 1625 |
| TaqI | TCGA | 3 cut(s) | 370, 589, 1315 |
| TatI | WGTACW | 1 cut(s) | 1264 |
| TauI | GCSGC | 1 cut(s) | 832 |
| TscAI | CASTG | 6 cut(s) | 153, 299, 332, 442, 2690, 2852 |
| TseFI | GTSAC | 7 cut(s) | 323, 1150, 1213, 1618, 1730, 1854, 1883 |
| TseI | GCWGC | 6 cut(s) | 299, 1187, 1190, 1571, 1610, 2522 |
| Tsp45I | GTSAC | 7 cut(s) | 323, 1150, 1213, 1618, 1730, 1854, 1883 |
| TspGWI | ACGGA | 3 cut(s) | 747, 1737, 2689 |
| TspMI | CCCGGG | 1 cut(s) | 1722 |
| TspRI | CASTG | 6 cut(s) | 153, 299, 332, 442, 2690, 2852 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1747 |
| Vha464I | CTTAAG | 1 cut(s) | 1735 |
| VneI | GTGCAC | 1 cut(s) | 2670 |
| VpaK11BI | GGWCC | 3 cut(s) | 5, 1001, 2488 |
| XapI | RAATTY | 5 cut(s) | 309, 820, 1016, 1067, 1386 |
| XbaI | TCTAGA | 2 cut(s) | 1009, 1940 |
| XceI | RCATGY | 6 cut(s) | 813, 1255, 1619, 1643, 1731, 2605 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 729 |
| XhoI | CTCGAG | 1 cut(s) | 588 |
| XmaI | CCCGGG | 1 cut(s) | 1722 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1386 |
| Zsp2I | ATGCAT | 1 cut(s) | 395 |