RLG00000024160
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0016448
Physical Location & Seq
Reverse (-)
32645235 .. 32647199
1965 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024160

Sequence Viewer

Length: 420 bp
ATGACGGGGTGCTGCTGCGGCGTGAATTGGATCCTGTCTGGAGAGGCGGAGGCCACGGCCATGGCGGGTCGGGTCGGGAGAATCCGAAAACGAGAGAAGGAAAGGAGAAAGGGACGGAAGGAGCGTCAGTTGGAGTTTGGTGGTGGAGTTAGAGACACTTTGGGAAGAATTACCAATATGTGGTATGTTTCTGCGGATTTAAGTGAAGAAGGTGCACGGAGTCCCGATTGTTTACAATTTGCACAGTTAGCCTCAGAGGCTGTTGATTCTGCAAAAAGTGGTAAAGATGTTCCCTTACCATTCCTTCATGGGACATACCCAGATTTCATGGAAAAGAATCCCAATCAGACCTATAAATCAAACAAAGTGCTCGGAAAACTTTATCGTCACTGGAAGTCCAAGGTGCTAGATGAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001172 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0003968 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009856 GO:0009875 GO:0009888 GO:0009892 GO:0009908 GO:0009987 GO:0010033 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0032774 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0044706 GO:0045087 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048507 GO:0048519 GO:0048544 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090567 GO:0097659 GO:0097747 GO:0098542 GO:0098586 GO:0098727 GO:0099402 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

140

Amino Acids

15.6

Weight (kDa)

9.25

Isoelectric Point (pI)

31.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdRP PF05183 50 - 130 4e-20 RNA dependent RNA polymerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 180
AciI CCGC 4 cut(s) 18, 47, 65, 194
AclWI GGATC 2 cut(s) 25, 38
AcoI YGGCCR 1 cut(s) 57
AfiI CCNNNNNNNGG 1 cut(s) 180
Alw21I GWGCWC 2 cut(s) 217, 372
Alw26I GTCTC 1 cut(s) 147
Alw44I GTGCAC 1 cut(s) 213
AlwI GGATC 2 cut(s) 25, 38
AlwNI CAGNNNCTG 1 cut(s) 260
AoxI GGCC 2 cut(s) 51, 57
ApaLI GTGCAC 1 cut(s) 213
ApeKI GCWGC 2 cut(s) 12, 15
BaeGI GKGCMC 1 cut(s) 217
BamHI GGATCC 1 cut(s) 30
Bbv12I GWGCWC 2 cut(s) 217, 372
BbvI GCAGC 1 cut(s) 2
BceAI ACGGC 1 cut(s) 72
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 1 cut(s) 407
BglI GCCNNNNNGGC 1 cut(s) 257
BisI GCNGC 3 cut(s) 13, 16, 19
BlsI GCNGC 3 cut(s) 14, 17, 20
BmiI GGNNCC 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 60
BsaJI CCNNGG 3 cut(s) 54, 60, 399
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 1 cut(s) 180
Bse1I ACTGG 1 cut(s) 395
BseDI CCNNGG 3 cut(s) 54, 60, 399
BseLI CCNNNNNNNGG 1 cut(s) 180
BseMII CTCAG 1 cut(s) 267
BseNI ACTGG 1 cut(s) 395
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 1 cut(s) 2
BshFI GGCC 2 cut(s) 53, 59
BsiHKAI GWGCWC 2 cut(s) 217, 372
BslFI GGGAC 3 cut(s) 126, 207, 325
BslI CCNNNNNNNGG 1 cut(s) 180
BsmAI GTCTC 1 cut(s) 147
BsmFI GGGAC 3 cut(s) 126, 207, 325
BsnI GGCC 2 cut(s) 53, 59
Bsp1286I GDGCHC 2 cut(s) 217, 372
Bsp143I GATC 1 cut(s) 30
Bsp19I CCATGG 1 cut(s) 60
BspACI CCGC 4 cut(s) 18, 47, 65, 194
BspANI GGCC 2 cut(s) 53, 59
BspCNI CTCAG 1 cut(s) 266
BspLI GGNNCC 1 cut(s) 32
BspPI GGATC 2 cut(s) 25, 38
BsrI ACTGG 1 cut(s) 395
BssECI CCNNGG 3 cut(s) 54, 60, 399
BssMI GATC 1 cut(s) 30
BssT1I CCWWGG 2 cut(s) 60, 399
Bst4CI ACNGT 1 cut(s) 246
BstDEI CTNAG 2 cut(s) 253, 417
BstDSI CCRYGG 2 cut(s) 54, 60
BstKTI GATC 1 cut(s) 33
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 1 cut(s) 30
BstMWI GCNNNNNNNGC 3 cut(s) 18, 248, 257
BstSLI GKGCMC 1 cut(s) 217
BstV1I GCAGC 1 cut(s) 2
BstX2I RGATCY 1 cut(s) 30
BstXI CCANNNNNNTGG 1 cut(s) 61
BstYI RGATCY 1 cut(s) 30
BsuRI GGCC 2 cut(s) 53, 59
BtgI CCRYGG 2 cut(s) 54, 60
BtsIMutI CAGTG 1 cut(s) 388
CaiI CAGNNNCTG 1 cut(s) 260
CseI GACGC 1 cut(s) 113
CviAII CATG 3 cut(s) 61, 308, 328
CviJI RGCY 4 cut(s) 53, 59, 251, 260
CviKI_1 RGCY 4 cut(s) 53, 59, 251, 260
DdeI CTNAG 2 cut(s) 253, 417
DpnI GATC 1 cut(s) 32
DpnII GATC 1 cut(s) 30
EaeI YGGCCR 1 cut(s) 57
EciI GGCGGA 1 cut(s) 62
Eco130I CCWWGG 2 cut(s) 60, 399
EcoT14I CCWWGG 2 cut(s) 60, 399
ErhI CCWWGG 2 cut(s) 60, 399
FaeI CATG 3 cut(s) 64, 311, 331
FaiI YATR 7 cut(s) 62, 179, 186, 309, 316, 329, 354
FaqI GGGAC 3 cut(s) 126, 207, 325
FatI CATG 3 cut(s) 60, 307, 327
FauI CCCGC 1 cut(s) 58
Fnu4HI GCNGC 3 cut(s) 13, 16, 19
Fsp4HI GCNGC 3 cut(s) 13, 16, 19
FspBI CTAG 1 cut(s) 407
GluI GCNGC 3 cut(s) 13, 16, 19
GsuI CTGGAG 1 cut(s) 60
HaeIII GGCC 2 cut(s) 53, 59
HgaI GACGC 1 cut(s) 113
Hin1II CATG 3 cut(s) 64, 311, 331
HinfI GANTC 5 cut(s) 81, 220, 266, 337, 413
Hpy166II GTNNAC 2 cut(s) 215, 233
Hpy188I TCNGA 4 cut(s) 86, 256, 348, 374
Hpy188III TCNNGA 3 cut(s) 39, 76, 224
Hpy8I GTNNAC 2 cut(s) 215, 233
HpyAV CCTTC 4 cut(s) 91, 112, 203, 314
HpyCH4III ACNGT 1 cut(s) 246
HpyCH4V TGCA 3 cut(s) 215, 242, 272
HpyF10VI GCNNNNNNNGC 3 cut(s) 18, 248, 257
HpyF3I CTNAG 2 cut(s) 253, 417
Hsp92II CATG 3 cut(s) 64, 311, 331
Kzo9I GATC 1 cut(s) 30
LmnI GCTCC 1 cut(s) 121
LpnPI CCDG 4 cut(s) 24, 47, 333, 376
Lsp1109I GCAGC 1 cut(s) 2
MaeI CTAG 1 cut(s) 407
MaeIII GTNAC 1 cut(s) 386
MalI GATC 1 cut(s) 32
MboI GATC 1 cut(s) 30
MboII GAAGA 2 cut(s) 177, 218
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 2 cut(s) 217, 372
MluCI AATT 3 cut(s) 25, 168, 236
MlyI GAGTC 1 cut(s) 229
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 4 cut(s) 37, 43, 250, 262
MseI TTAA 1 cut(s) 200
MslI CAYNNNNRTG 1 cut(s) 59
MwoI GCNNNNNNNGC 3 cut(s) 18, 248, 257
NcoI CCATGG 1 cut(s) 60
NdeII GATC 1 cut(s) 30
NlaIII CATG 3 cut(s) 64, 311, 331
NlaIV GGNNCC 1 cut(s) 32
NmuCI GTSAC 1 cut(s) 386
PcsI WCGNNNNNNNCGW 1 cut(s) 121
PfeI GAWTC 4 cut(s) 81, 266, 337, 413
PflMI CCANNNNNTGG 1 cut(s) 180
PkrI GCNGC 3 cut(s) 14, 17, 20
PleI GAGTC 1 cut(s) 228
PpsI GAGTC 1 cut(s) 228
PspN4I GGNNCC 1 cut(s) 32
PstNI CAGNNNCTG 1 cut(s) 260
PsuI RGATCY 1 cut(s) 30
RseI CAYNNNNRTG 1 cut(s) 59
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 3 cut(s) 13, 16, 19
Sau3AI GATC 1 cut(s) 30
SchI GAGTC 1 cut(s) 229
SduI GDGCHC 2 cut(s) 217, 372
SetI ASST 3 cut(s) 214, 353, 405
SmiMI CAYNNNNRTG 1 cut(s) 59
Sse9I AATT 3 cut(s) 25, 168, 236
SsiI CCGC 4 cut(s) 18, 47, 65, 194
SspMI CTAG 1 cut(s) 407
StyI CCWWGG 2 cut(s) 60, 399
TaaI ACNGT 1 cut(s) 246
TasI AATT 3 cut(s) 25, 168, 236
TauI GCSGC 1 cut(s) 21
TfiI GAWTC 4 cut(s) 81, 266, 337, 413
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TscAI CASTG 1 cut(s) 395
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 2 cut(s) 12, 15
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 2 cut(s) 296, 316
TspGWI ACGGA 2 cut(s) 130, 232
TspRI CASTG 1 cut(s) 395
Van91I CCANNNNNTGG 1 cut(s) 180
VneI GTGCAC 1 cut(s) 213
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.