Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0010241

Physical Location & Seq

Genomic Coordinates Reverse (-)

34296035 .. 34297901

Gene Structure 1867 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ45156

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1620 bp

ATGCCCAAGCCCTTTTTCTTCAAGCCATTTTTCTTTCTATATCGCATTTTCTTTCGGAAATCCAAACGTTTTCAGAGAAGTGCAAGCAACAGCCATGCAAGCCAGTCCAAAAGTCATATACACGCATTTGATAATTTTGCCCATAGATACCCAGACCTCAAGAACCTATCTCTCATCATCTTCAACATGGAAGAAGCTTTGCTGAAATCCTCTTCTTTGTTCCCCTACTTCATGCTTGTGGCTTTTGAGGCTGGAAGTCTATTAAGGGCCTTTGTTCTTTTTCTTTTGTACCCTCTTATGTTCTTGGTCAGTGAAGAAGTAGGGTTGAAAATCATGGTCATGGTCTGCTTCTTTGGGATTAAGAAGGAGAGCTTCAGAATTGGAAGAGCTGTGTTGCCTAAGTTCTTCTTAGAAGATGTTGGGTTGGAAAGCTTTGAGGTGCTTCAGAGAGCTGGGAAAAAGAAAGTTGGCGTCAGTAATCTTCCTCAAGTTTTGATCGAGACCTTCTTGAAAGACTATCTGGAGATTGATGTGGTGATTGGGAGGGAGCTAAAGGTGTTTGGTGGATACTTTTTGGGTCTCATGGAAGAAAAGAAGAATACTAATTTGACTATCCTGGAAGAACGTGGTGGACACGACAAAATGGGTAGCTCGGATATAATTGGCATTACTTCCTTCAATCAATGCCTTGATGACCCAATATTCTCTTATTGCAAGGATGTTTACCGAGTGAGAAGCTCTGATAAGAGGAGCTGGAAGAACCTGCCCAAAGAGAAGTACCCAAAGAAACTCATCTTCCATGATGGCAGACTCGCTCTCAAACCAACGCCACTAGAAACTGTAGCAATCTTCATGTGGGCCCCAGTTGGGTTTGTACTTGCAGTCGTCAGAATCCTCCTCGGCATATCGCTTCCCTACGAAGTGTCGTCTCCTCTGCTCGCCTTCACAGGACTGCGGCTGACGGTTAGGACGCCACAAACTGAAAGCCTCGTTCAAAAGGACCCTCGACTTCCAAACGATAAGCCCAAAGGCGTCCTCTACGTTTGTAACCATAGAACACTACTAGACCCTCTGTACTTATGTTTCGCAATGGGGAAAAATCTCCATGCGGTTACGTACAGTCTAAGCAAAATGTCGGAGATACTAGCTCCGATCAAGACCGTCCGATTAGCAAGAAATCGACAGCAAGATCAGGATATGATGAAAAGGTTGTTGAGTCAAGGGGACCTGGTGGTTTGCCCCGAAGGAACGACATGTCGTGAGCCCTACCTACTCAGGTTCAGCCCGTTGTTTTCAGAAATAAGTGACGAAATTGTTCCCGTTGCGGTTGACTCCCACGTTAGCATGTTTCACGGTACGACGGCTGGTGGACTCAAGAGTCTGGATCCGGTCTTCTTCCTCCTCAACCCTTCACCGATCTACACCGTGCAGTTGCTGGAGAAGGTGTCATCGGAAGTGTCCTCGTGTCGTGATGGGTCGACGTCGAGGTTTGATGTGGCTAACTACGTACAAAGGGAGCTTGGTAAGGTTTTGGGGTTTGAATGTACAAAGCTTACAAGGAGAGAGAAGTACTTGATTTTGGCAGGAAATGAAGGCAAAGCTGGTTGTAAAACTGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009987 GO:0010143 GO:0016020 GO:0016311 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0043170 GO:0044237 GO:0071704 GO:0090447 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

Acyltransferase

Protein Analysis

539

Amino Acids

61.18

Weight (kDa)

9.33

Isoelectric Point (pI)

39.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAD_RAM2_N	PF23270	59 - 244	2.7e-67	Glycerol-3-phosphate acyltransferase RAM2-like, HAD-like domain
Acyltransferase	PF01553	342 - 443	3.2e-08	Acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010241)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02390 AT4G01950 AT4G01950
fragaria_vesca	FvH4_1g26880
malus_domestica	MD09G1285900.v1.1 MD17G1278500.v1.1
prunus_persica	Prupe.3G007500_v2.0.a1
pyrus_communis	pycom09g19320 pycom17g27610
rosa_chinensis	RchiOBHm_Chr3g0487121
rosa_laevigata	RLG00000023031
rosa_multiflora	Rmu_sc0003329.1_g000010 Rmu_sc0008479.1_g000005 Rmu_sc0032997.1_g000001
rosa_roxburghii	Rroxscaffold_6G00395170
rosa_rugosa	Rorug03G0231400
rosa_samantha	Rh3AG279500 Rh3BG315500 Rh3CG313800 Rh3DG310600
rosa_wichuraiana	Rw3G024770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1377
AatII	GACGTC	1 cut(s)	1484
Acc36I	ACCTGC	1 cut(s)	771
AccI	GTMKAC	1 cut(s)	1478
AciI	CCGC	3 cut(s)	955, 1109, 1325
AclI	AACGTT	1 cut(s)	67
AclWI	GGATC	2 cut(s)	1379, 1392
AcuI	CTGAAG	2 cut(s)	358, 428
AcyI	GRCGYC	4 cut(s)	471, 971, 1032, 1481
AfaI	GTAC	9 cut(s)	290, 779, 876, 1076, 1118, 1357, 1509, 1546, 1571
AfiI	CCNNNNNNNGG	1 cut(s)	867
AflIII	ACRYGT	1 cut(s)	1253
AgsI	TTSAA	7 cut(s)	22, 184, 328, 511, 679, 995, 1541
AjnI	CCWGG	2 cut(s)	615, 1227
AjuI	GAANNNNNNNTTGG	1 cut(s)	31
Alw26I	GTCTC	3 cut(s)	494, 584, 933
AlwI	GGATC	2 cut(s)	1379, 1392
AlwNI	CAGNNNCTG	1 cut(s)	1435
AoxI	GGCC	2 cut(s)	267, 858
ApaI	GGGCCC	1 cut(s)	862
Asp700I	GAANNNNTTC	1 cut(s)	1314
AspS9I	GGNCC	5 cut(s)	267, 858, 859, 1000, 1225
AsuHPI	GGTGA	2 cut(s)	547, 1404
AvaII	GGWCC	2 cut(s)	1000, 1225
BaeGI	GKGCMC	1 cut(s)	862
BamHI	GGATCC	1 cut(s)	1384
BanII	GRGCYC	2 cut(s)	862, 1266
BauI	CACGAG	1 cut(s)	1462
BbsI	GAAGAC	1 cut(s)	1384
BccI	CCATC	2 cut(s)	797, 1466
BceAI	ACGGC	1 cut(s)	1377
BciT130I	CCWGG	2 cut(s)	617, 1229
BciVI	GTATCC	1 cut(s)	560
BcoDI	GTCTC	3 cut(s)	494, 584, 933
BfaI	CTAG	3 cut(s)	833, 1064, 1145
BfmI	CTRYAG	1 cut(s)	840
BfuAI	ACCTGC	1 cut(s)	771
BfuI	GTATCC	1 cut(s)	560
BisI	GCNGC	1 cut(s)	956
BlsI	GCNGC	1 cut(s)	957
BmcAI	AGTACT	1 cut(s)	1571
Bme1390I	CCNGG	2 cut(s)	617, 1229
Bme18I	GGWCC	2 cut(s)	1000, 1225
BmgT120I	GGNCC	5 cut(s)	267, 858, 859, 1000, 1225
BmiI	GGNNCC	5 cut(s)	860, 861, 1002, 1226, 1386
BmrFI	CCNGG	2 cut(s)	617, 1229
BmrI	ACTGGG	1 cut(s)	857
BmuI	ACTGGG	1 cut(s)	857
BpiI	GAAGAC	1 cut(s)	1384
BpmI	CTGGAG	2 cut(s)	542, 1457
BpuEI	CTTGAG	3 cut(s)	143, 471, 1358
BsaAI	YACGTR	2 cut(s)	1116, 1507
BsaHI	GRCGYC	4 cut(s)	471, 971, 1032, 1481
BsaI	GGTCTC	2 cut(s)	494, 584
BsaJI	CCNNGG	1 cut(s)	898
BsaWI	WCCGGW	1 cut(s)	1387
BsaXI	ACNNNNNCTCC	2 cut(s)	1430, 1460
Bsc4I	CCNNNNNNNGG	1 cut(s)	867
Bse1I	ACTGG	2 cut(s)	103, 863
Bse3DI	GCAATG	1 cut(s)	1095
BseBI	CCWGG	2 cut(s)	617, 1229
BseDI	CCNNGG	1 cut(s)	898
BseGI	GGATG	1 cut(s)	724
BseLI	CCNNNNNNNGG	1 cut(s)	867
BseMI	GCAATG	1 cut(s)	1095
BseMII	CTCAG	1 cut(s)	1288
BseNI	ACTGG	2 cut(s)	103, 863
BseRI	GAGGAG	4 cut(s)	763, 887, 921, 1391
BseSI	GKGCMC	1 cut(s)	862
BseYI	CCCAGC	1 cut(s)	452
BsgI	GTGCAG	1 cut(s)	1448
BshFI	GGCC	2 cut(s)	269, 860
BsiSI	CCGG	1 cut(s)	1388
BslFI	GGGAC	1 cut(s)	1238
BslI	CCNNNNNNNGG	1 cut(s)	867
BsmAI	GTCTC	3 cut(s)	494, 584, 933
BsmBI	CGTCTC	1 cut(s)	933
BsmFI	GGGAC	1 cut(s)	1238
BsnI	GGCC	2 cut(s)	269, 860
Bso31I	GGTCTC	2 cut(s)	494, 584
Bsp120I	GGGCCC	1 cut(s)	858
Bsp1286I	GDGCHC	2 cut(s)	862, 1266
Bsp1407I	TGTACA	1 cut(s)	1544
Bsp143I	GATC	5 cut(s)	495, 1152, 1189, 1384, 1416
BspACI	CCGC	3 cut(s)	955, 1109, 1325
BspANI	GGCC	2 cut(s)	269, 860
BspCNI	CTCAG	1 cut(s)	1287
BspLI	GGNNCC	5 cut(s)	860, 861, 1002, 1226, 1386
BspMI	ACCTGC	1 cut(s)	771
BspPI	GGATC	2 cut(s)	1379, 1392
BspQI	GCTCTTC	1 cut(s)	379
BspTNI	GGTCTC	2 cut(s)	494, 584
BsrDI	GCAATG	1 cut(s)	1095
BsrGI	TGTACA	1 cut(s)	1544
BsrI	ACTGG	2 cut(s)	103, 863
BssECI	CCNNGG	1 cut(s)	898
BssMI	GATC	5 cut(s)	495, 1152, 1189, 1384, 1416
BssNI	GRCGYC	4 cut(s)	471, 971, 1032, 1481
BssSI	CACGAG	1 cut(s)	1462
Bst2BI	CACGAG	1 cut(s)	1462
Bst2UI	CCWGG	2 cut(s)	617, 1229
Bst4CI	ACNGT	6 cut(s)	841, 964, 1121, 1162, 1355, 1426
Bst6I	CTCTTC	2 cut(s)	217, 379
BstACI	GRCGYC	4 cut(s)	471, 971, 1032, 1481
BstAUI	TGTACA	1 cut(s)	1544
BstBAI	YACGTR	2 cut(s)	1116, 1507
BstC8I	GCNNGC	3 cut(s)	85, 100, 939
BstDEI	CTNAG	4 cut(s)	399, 409, 1124, 1274
BstF5I	GGATG	1 cut(s)	724
BstKTI	GATC	5 cut(s)	498, 1155, 1192, 1387, 1419
BstMAI	GTCTC	3 cut(s)	494, 584, 933
BstMBI	GATC	5 cut(s)	495, 1152, 1189, 1384, 1416
BstMWI	GCNNNNNNNGC	2 cut(s)	99, 248
BstNI	CCWGG	2 cut(s)	617, 1229
BstNSI	RCATGY	2 cut(s)	1257, 1348
BstSCI	CCNGG	2 cut(s)	615, 1227
BstSFI	CTRYAG	1 cut(s)	840
BstSLI	GKGCMC	1 cut(s)	862
BstSNI	TACGTA	2 cut(s)	1116, 1507
BstV2I	GAAGAC	1 cut(s)	1384
BstX2I	RGATCY	1 cut(s)	1384
BstYI	RGATCY	1 cut(s)	1384
BsuI	GTATCC	1 cut(s)	560
BsuRI	GGCC	2 cut(s)	269, 860
BtsCI	GGATG	1 cut(s)	724
BtsIMutI	CAGTG	1 cut(s)	316
BveI	ACCTGC	1 cut(s)	771
Cac8I	GCNNGC	3 cut(s)	85, 100, 939
CaiI	CAGNNNCTG	1 cut(s)	1435
Cfr13I	GGNCC	5 cut(s)	267, 858, 859, 1000, 1225
CseI	GACGC	3 cut(s)	460, 979, 1021
CsiI	ACCWGGT	1 cut(s)	1227
Csp6I	GTAC	9 cut(s)	289, 778, 875, 1075, 1117, 1356, 1508, 1545, 1570
CviQI	GTAC	9 cut(s)	289, 778, 875, 1075, 1117, 1356, 1508, 1545, 1570
DdeI	CTNAG	4 cut(s)	399, 409, 1124, 1274
DpnI	GATC	5 cut(s)	497, 1154, 1191, 1386, 1418
DpnII	GATC	5 cut(s)	495, 1152, 1189, 1384, 1416
DrdI	GACNNNNNNGTC	1 cut(s)	1377
DseDI	GACNNNNNNGTC	1 cut(s)	1377
Eam1104I	CTCTTC	2 cut(s)	217, 379
EarI	CTCTTC	2 cut(s)	217, 379
Eco105I	TACGTA	2 cut(s)	1116, 1507
Eco24I	GRGCYC	2 cut(s)	862, 1266
Eco31I	GGTCTC	2 cut(s)	494, 584
Eco47I	GGWCC	2 cut(s)	1000, 1225
Eco57I	CTGAAG	2 cut(s)	358, 428
EcoO109I	RGGNCCY	4 cut(s)	267, 859, 1000, 1225
EcoRII	CCWGG	2 cut(s)	615, 1227
EcoT38I	GRGCYC	2 cut(s)	862, 1266
Esp3I	CGTCTC	1 cut(s)	933
FalI	AAGNNNNNCTT	4 cut(s)	356, 388, 392, 424
FaqI	GGGAC	1 cut(s)	1238
FblI	GTMKAC	1 cut(s)	1478
Fnu4HI	GCNGC	1 cut(s)	956
FokI	GGATG	1 cut(s)	731
FriOI	GRGCYC	2 cut(s)	862, 1266
Fsp4HI	GCNGC	1 cut(s)	956
FspBI	CTAG	3 cut(s)	833, 1064, 1145
GluI	GCNGC	1 cut(s)	956
GsaI	CCCAGC	1 cut(s)	456
GsuI	CTGGAG	2 cut(s)	542, 1457
HaeIII	GGCC	2 cut(s)	269, 860
HapII	CCGG	1 cut(s)	1388
HgaI	GACGC	3 cut(s)	460, 979, 1021
Hin1I	GRCGYC	4 cut(s)	471, 971, 1032, 1481
HincII	GTYRAC	2 cut(s)	1330, 1479
HindII	GTYRAC	2 cut(s)	1330, 1479
HindIII	AAGCTT	3 cut(s)	195, 430, 1550
HinfI	GANTC	6 cut(s)	810, 891, 1216, 1331, 1371, 1378
HpaII	CCGG	1 cut(s)	1388
HphI	GGTGA	2 cut(s)	547, 1404
Hpy166II	GTNNAC	5 cut(s)	632, 724, 1330, 1370, 1479
Hpy8I	GTNNAC	5 cut(s)	632, 724, 1330, 1370, 1479
Hpy99I	CGWCG	3 cut(s)	1363, 1483, 1486
HpyAV	CCTTC	8 cut(s)	358, 514, 685, 952, 1238, 1419, 1435, 1586
HpyCH4III	ACNGT	6 cut(s)	841, 964, 1121, 1162, 1355, 1426
HpyCH4IV	ACGT	7 cut(s)	67, 625, 1041, 1115, 1338, 1481, 1506
HpyCH4V	TGCA	6 cut(s)	83, 98, 714, 881, 1429, 1616
HpyF10VI	GCNNNNNNNGC	2 cut(s)	99, 248
HpyF3I	CTNAG	4 cut(s)	399, 409, 1124, 1274
HpySE526I	ACGT	7 cut(s)	67, 625, 1041, 1115, 1338, 1481, 1506
Hsp92I	GRCGYC	4 cut(s)	471, 971, 1032, 1481
Kzo9I	GATC	5 cut(s)	495, 1152, 1189, 1384, 1416
LguI	GCTCTTC	1 cut(s)	379
LmnI	GCTCC	4 cut(s)	547, 750, 1153, 1516
MabI	ACCWGGT	1 cut(s)	1227
MaeI	CTAG	3 cut(s)	833, 1064, 1145
MaeII	ACGT	7 cut(s)	67, 625, 1041, 1115, 1338, 1481, 1506
MaeIII	GTNAC	3 cut(s)	1046, 1111, 1304
MalI	GATC	5 cut(s)	497, 1154, 1191, 1386, 1418
MboI	GATC	5 cut(s)	495, 1152, 1189, 1384, 1416
MflI	RGATCY	1 cut(s)	1384
MhlI	GDGCHC	2 cut(s)	862, 1266
MluCI	AATT	5 cut(s)	133, 378, 604, 660, 1311
MlyI	GAGTC	5 cut(s)	804, 1225, 1325, 1365, 1387
MmeI	TCCRAC	2 cut(s)	405, 1116
MroXI	GAANNNNTTC	1 cut(s)	1314
MseI	TTAA	2 cut(s)	263, 360
MslI	CAYNNNNRTG	2 cut(s)	236, 338
MspI	CCGG	1 cut(s)	1388
MspR9I	CCNGG	2 cut(s)	617, 1229
MvaI	CCWGG	2 cut(s)	617, 1229
MwoI	GCNNNNNNNGC	2 cut(s)	99, 248
NdeII	GATC	5 cut(s)	495, 1152, 1189, 1384, 1416
NlaIV	GGNNCC	5 cut(s)	860, 861, 1002, 1226, 1386
NmeAIII	GCCGAG	1 cut(s)	879
NmuCI	GTSAC	1 cut(s)	1304
NspI	RCATGY	2 cut(s)	1257, 1348
PciI	ACATGT	1 cut(s)	1253
PciSI	GCTCTTC	1 cut(s)	379
PdmI	GAANNNNTTC	1 cut(s)	1314
PfeI	GAWTC	1 cut(s)	891
PfoI	TCCNGGA	1 cut(s)	615
PkrI	GCNGC	1 cut(s)	957
PleI	GAGTC	5 cut(s)	804, 1224, 1325, 1365, 1386
PpsI	GAGTC	5 cut(s)	804, 1224, 1325, 1365, 1386
Ppu21I	YACGTR	2 cut(s)	1116, 1507
PpuMI	RGGWCCY	2 cut(s)	1000, 1225
PscI	ACATGT	1 cut(s)	1253
Psp1406I	AACGTT	1 cut(s)	67
Psp5II	RGGWCCY	2 cut(s)	1000, 1225
Psp6I	CCWGG	2 cut(s)	615, 1227
PspFI	CCCAGC	1 cut(s)	452
PspGI	CCWGG	2 cut(s)	615, 1227
PspN4I	GGNNCC	5 cut(s)	860, 861, 1002, 1226, 1386
PspOMI	GGGCCC	1 cut(s)	858
PspPI	GGNCC	5 cut(s)	267, 858, 859, 1000, 1225
PspPPI	RGGWCCY	2 cut(s)	1000, 1225
PstNI	CAGNNNCTG	1 cut(s)	1435
PsuI	RGATCY	1 cut(s)	1384
RsaI	GTAC	9 cut(s)	290, 779, 876, 1076, 1118, 1357, 1509, 1546, 1571
RsaNI	GTAC	9 cut(s)	289, 778, 875, 1075, 1117, 1356, 1508, 1545, 1570
RseI	CAYNNNNRTG	2 cut(s)	236, 338
SalI	GTCGAC	1 cut(s)	1477
SapI	GCTCTTC	1 cut(s)	379
SaqAI	TTAA	2 cut(s)	263, 360
SatI	GCNGC	1 cut(s)	956
Sau3AI	GATC	5 cut(s)	495, 1152, 1189, 1384, 1416
Sau96I	GGNCC	5 cut(s)	267, 858, 859, 1000, 1225
ScaI	AGTACT	1 cut(s)	1571
SchI	GAGTC	5 cut(s)	804, 1225, 1325, 1365, 1387
ScrFI	CCNGG	2 cut(s)	617, 1229
SduI	GDGCHC	2 cut(s)	862, 1266
SexAI	ACCWGGT	1 cut(s)	1227
SfcI	CTRYAG	1 cut(s)	840
SinI	GGWCC	2 cut(s)	1000, 1225
SmiMI	CAYNNNNRTG	2 cut(s)	236, 338
SmlI	CTYRAG	3 cut(s)	158, 486, 1373
SmoI	CTYRAG	3 cut(s)	158, 486, 1373
SnaBI	TACGTA	2 cut(s)	1116, 1507
Sse9I	AATT	5 cut(s)	133, 378, 604, 660, 1311
SsiI	CCGC	3 cut(s)	955, 1109, 1325
SspI	AATATT	1 cut(s)	702
SspMI	CTAG	3 cut(s)	833, 1064, 1145
StyD4I	CCNGG	2 cut(s)	615, 1227
TaaI	ACNGT	6 cut(s)	841, 964, 1121, 1162, 1355, 1426
TaiI	ACGT	7 cut(s)	70, 628, 1044, 1118, 1341, 1484, 1509
TaqI	TCGA	5 cut(s)	498, 1006, 1180, 1478, 1484
TasI	AATT	5 cut(s)	133, 378, 604, 660, 1311
TatI	WGTACW	4 cut(s)	874, 1074, 1544, 1569
TauI	GCSGC	1 cut(s)	958
TfiI	GAWTC	1 cut(s)	891
Tru1I	TTAA	2 cut(s)	263, 360
Tru9I	TTAA	2 cut(s)	263, 360
TscAI	CASTG	1 cut(s)	316
TseFI	GTSAC	1 cut(s)	1304
Tsp45I	GTSAC	1 cut(s)	1304
TspDTI	ATGAA	4 cut(s)	220, 841, 1217, 1605
TspRI	CASTG	1 cut(s)	316
VpaK11BI	GGWCC	2 cut(s)	1000, 1225
XceI	RCATGY	2 cut(s)	1257, 1348
XmiI	GTMKAC	1 cut(s)	1478
XmnI	GAANNNNTTC	1 cut(s)	1314
XspI	CTAG	3 cut(s)	833, 1064, 1145
ZraI	GACGTC	1 cut(s)	1482
ZrmI	AGTACT	1 cut(s)	1571

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.