RchiOBHm_Chr3g0491441

Alkaline and neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0020520
Physical Location & Seq
Reverse (-)
38164570 .. 38165289
720 bp
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UTR
Exon/CDS
Intron
PRQ45443

Sequence Viewer

Length: 495 bp
ATGTCGAAACTCCAAAAAGAAGAGAAAGAAGCAGAGGAGGCGGCCGCTCGGCCTCCGCCTTCTGCTCCGTCGCCGGCGACGAGTGCGACTCTGAGTGACTCAACGAAATCAAAAGAAGGATCCGCAACCTATGACAAGAGCGGGAGTGCCGAATTGGCGAAGAAAGGTTCATATTCTCTGGAGAAGTGTATGGATATGTGCGCGACACCGCCGGTGAAACAGCAGAGGCACGACGAAGCTCCAGATAGCGCACCGAAGAAGACGATGTCGCATCAAAGTTTGAAGGAATCTTCGGCGGTTATGGTGGAGGAGGCCTGGGGGAGACTGAAGAAGTCCTTCGTTTACTACAGAGGCAAGCCGGTGGCGATTCTTGCTGCTACGGATCCAATGGCGGAGGCTTTGAATTACAATCAGATGTTAGTAACCCCTGGATATAATGATTCTCCAACTTGTTTTTACATTGTGAAGTGCTTGGGGTTAGAATCAGTTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

164

Amino Acids

17.76

Weight (kDa)

6.73

Isoelectric Point (pI)

56.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020520)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0491441
rosa_laevigata RLG00000022841
rosa_multiflora Rmu_sc0006034.1_g000004
rosa_rugosa Rorug03G0249400
rosa_samantha Rh3AG298600 Rh3DG333100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 47, 141
AccII CGCG 1 cut(s) 203
AciI CCGC 8 cut(s) 41, 45, 56, 123, 141, 209, 296, 392
AclWI GGATC 4 cut(s) 114, 127, 377, 390
AcoI YGGCCR 1 cut(s) 42
AcuI CTGAAG 1 cut(s) 347
AgsI TTSAA 2 cut(s) 283, 403
AjnI CCWGG 2 cut(s) 314, 427
AluBI AGCT 1 cut(s) 239
AluI AGCT 1 cut(s) 239
Alw26I GTCTC 1 cut(s) 316
AlwI GGATC 4 cut(s) 114, 127, 377, 390
AoxI GGCC 3 cut(s) 42, 50, 312
ApeKI GCWGC 1 cut(s) 374
Asp700I GAANNNNTTC 1 cut(s) 335
AspLEI GCGC 2 cut(s) 203, 251
AsuHPI GGTGA 1 cut(s) 226
BamHI GGATCC 2 cut(s) 119, 382
BbsI GAAGAC 1 cut(s) 266
BbvI GCAGC 1 cut(s) 361
BciT130I CCWGG 2 cut(s) 316, 429
BcoDI GTCTC 1 cut(s) 316
BfaI CTAG 1 cut(s) 493
BfmI CTRYAG 1 cut(s) 346
BglI GCCNNNNNGGC 1 cut(s) 155
BisI GCNGC 3 cut(s) 42, 45, 375
BlsI GCNGC 3 cut(s) 43, 46, 376
Bme1390I CCNGG 2 cut(s) 316, 429
BmiI GGNNCC 2 cut(s) 121, 384
BmrFI CCNGG 2 cut(s) 316, 429
BmsI GCATC 1 cut(s) 280
BpiI GAAGAC 1 cut(s) 266
BplI GAGNNNNNCTC 2 cut(s) 73, 105
BpmI CTGGAG 2 cut(s) 200, 225
BsaJI CCNNGG 2 cut(s) 315, 427
Bse118I RCCGGY 3 cut(s) 73, 211, 358
BseBI CCWGG 2 cut(s) 316, 429
BseDI CCNNGG 2 cut(s) 315, 427
BseMII CTCAG 1 cut(s) 83
BseRI GAGGAG 2 cut(s) 50, 323
BseX3I CGGCCG 1 cut(s) 42
BseXI GCAGC 1 cut(s) 361
Bsh1236I CGCG 1 cut(s) 203
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 3 cut(s) 44, 52, 314
BsiEI CGRYCG 1 cut(s) 45
BsiSI CCGG 3 cut(s) 74, 212, 359
BsmAI GTCTC 1 cut(s) 316
BsnI GGCC 3 cut(s) 44, 52, 314
Bsp143I GATC 2 cut(s) 119, 382
BspACI CCGC 8 cut(s) 41, 45, 56, 123, 141, 209, 296, 392
BspANI GGCC 3 cut(s) 44, 52, 314
BspCNI CTCAG 1 cut(s) 84
BspFNI CGCG 1 cut(s) 203
BspLI GGNNCC 2 cut(s) 121, 384
BspPI GGATC 4 cut(s) 114, 127, 377, 390
BsrBI CCGCTC 2 cut(s) 47, 141
BsrFI RCCGGY 3 cut(s) 73, 211, 358
BssAI RCCGGY 3 cut(s) 73, 211, 358
BssECI CCNNGG 2 cut(s) 315, 427
BssMI GATC 2 cut(s) 119, 382
Bst2UI CCWGG 2 cut(s) 316, 429
Bst6I CTCTTC 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 75, 356
BstDEI CTNAG 1 cut(s) 92
BstFNI CGCG 1 cut(s) 203
BstHHI GCGC 2 cut(s) 203, 251
BstKTI GATC 2 cut(s) 122, 385
BstMAI GTCTC 1 cut(s) 316
BstMBI GATC 2 cut(s) 119, 382
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 4 cut(s) 38, 83, 155, 371
BstNI CCWGG 2 cut(s) 316, 429
BstSCI CCNGG 2 cut(s) 314, 427
BstSFI CTRYAG 1 cut(s) 346
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 1 cut(s) 361
BstV2I GAAGAC 1 cut(s) 266
BstX2I RGATCY 2 cut(s) 119, 382
BstYI RGATCY 2 cut(s) 119, 382
BstZI CGGCCG 1 cut(s) 42
BsuRI GGCC 3 cut(s) 44, 52, 314
Cac8I GCNNGC 2 cut(s) 75, 356
CciNI GCGGCCGC 1 cut(s) 42
CfoI GCGC 2 cut(s) 203, 251
Cfr10I RCCGGY 3 cut(s) 73, 211, 358
CviJI RGCY 6 cut(s) 44, 52, 239, 314, 358, 398
CviKI_1 RGCY 6 cut(s) 44, 52, 239, 314, 358, 398
DdeI CTNAG 1 cut(s) 92
DpnI GATC 2 cut(s) 121, 384
DpnII GATC 2 cut(s) 119, 382
EaeI YGGCCR 1 cut(s) 42
EagI CGGCCG 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
EciI GGCGGA 2 cut(s) 45, 407
EclXI CGGCCG 1 cut(s) 42
Eco147I AGGCCT 1 cut(s) 314
Eco52I CGGCCG 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 347
EcoRII CCWGG 2 cut(s) 314, 427
FaiI YATR 6 cut(s) 132, 172, 191, 197, 302, 435
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FauI CCCGC 1 cut(s) 134
Fnu4HI GCNGC 3 cut(s) 42, 45, 375
Fsp4HI GCNGC 3 cut(s) 42, 45, 375
FspBI CTAG 1 cut(s) 493
GlaI GCGC 2 cut(s) 202, 250
GluI GCNGC 3 cut(s) 42, 45, 375
GsuI CTGGAG 2 cut(s) 200, 225
HaeIII GGCC 3 cut(s) 44, 52, 314
HapII CCGG 3 cut(s) 74, 212, 359
HhaI GCGC 2 cut(s) 203, 251
Hin6I GCGC 2 cut(s) 201, 249
HinP1I GCGC 2 cut(s) 201, 249
HinfI GANTC 6 cut(s) 88, 98, 287, 367, 440, 482
HpaII CCGG 3 cut(s) 74, 212, 359
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 2 cut(s) 93, 414
Hpy188III TCNNGA 2 cut(s) 179, 242
Hpy8I GTNNAC 1 cut(s) 343
Hpy99I CGWCG 3 cut(s) 73, 82, 236
HpyAV CCTTC 4 cut(s) 69, 110, 277, 346
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 83, 155, 371
HpyF3I CTNAG 1 cut(s) 92
HspAI GCGC 2 cut(s) 201, 249
KroI GCCGGC 1 cut(s) 73
KroNI GCCGGC 1 cut(s) 75
Kzo9I GATC 2 cut(s) 119, 382
LmnI GCTCC 2 cut(s) 70, 244
LpnPI CCDG 9 cut(s) 87, 164, 225, 255, 301, 328, 372, 414, 441
Lsp1109I GCAGC 1 cut(s) 361
LweI GCATC 1 cut(s) 280
MaeI CTAG 1 cut(s) 493
MaeIII GTNAC 2 cut(s) 95, 421
MalI GATC 2 cut(s) 121, 384
MbiI CCGCTC 2 cut(s) 47, 141
MboI GATC 2 cut(s) 119, 382
MboII GAAGA 6 cut(s) 32, 172, 268, 271, 282, 340
MflI RGATCY 2 cut(s) 119, 382
MluCI AATT 2 cut(s) 152, 403
MlyI GAGTC 2 cut(s) 82, 92
MmeI TCCRAC 1 cut(s) 470
MnlI CCTC 8 cut(s) 28, 31, 63, 219, 301, 304, 344, 388
MreI CGCCGGCG 1 cut(s) 73
MroNI GCCGGC 1 cut(s) 73
MroXI GAANNNNTTC 1 cut(s) 335
MspI CCGG 3 cut(s) 74, 212, 359
MspR9I CCNGG 2 cut(s) 316, 429
MvaI CCWGG 2 cut(s) 316, 429
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 4 cut(s) 38, 83, 155, 371
NaeI GCCGGC 1 cut(s) 75
NdeII GATC 2 cut(s) 119, 382
NgoMIV GCCGGC 1 cut(s) 73
NlaIV GGNNCC 2 cut(s) 121, 384
NmeAIII GCCGAG 1 cut(s) 28
NmuCI GTSAC 1 cut(s) 95
NotI GCGGCCGC 1 cut(s) 42
PceI AGGCCT 1 cut(s) 314
PcsI WCGNNNNNNNCGW 1 cut(s) 77
PdiI GCCGGC 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 335
PfeI GAWTC 4 cut(s) 287, 367, 440, 482
PflFI GACNNNGTC 1 cut(s) 265
PkrI GCNGC 3 cut(s) 43, 46, 376
PleI GAGTC 2 cut(s) 82, 92
PpsI GAGTC 2 cut(s) 82, 92
Psp6I CCWGG 2 cut(s) 314, 427
PspGI CCWGG 2 cut(s) 314, 427
PspN4I GGNNCC 2 cut(s) 121, 384
PsuI RGATCY 2 cut(s) 119, 382
PsyI GACNNNGTC 1 cut(s) 265
SatI GCNGC 3 cut(s) 42, 45, 375
Sau3AI GATC 2 cut(s) 119, 382
SchI GAGTC 2 cut(s) 82, 92
ScrFI CCNGG 2 cut(s) 316, 429
SetI ASST 3 cut(s) 131, 169, 241
SfaNI GCATC 1 cut(s) 280
SfcI CTRYAG 1 cut(s) 346
SgrAI CRCCGGYG 2 cut(s) 73, 211
Sse9I AATT 2 cut(s) 152, 403
SseBI AGGCCT 1 cut(s) 314
SsiI CCGC 8 cut(s) 41, 45, 56, 123, 141, 209, 296, 392
SspMI CTAG 1 cut(s) 493
StuI AGGCCT 1 cut(s) 314
StyD4I CCNGG 2 cut(s) 314, 427
TaqI TCGA 1 cut(s) 5
TasI AATT 2 cut(s) 152, 403
TauI GCSGC 2 cut(s) 44, 47
TfiI GAWTC 4 cut(s) 287, 367, 440, 482
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 1 cut(s) 374
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 1 cut(s) 159
TspGWI ACGGA 2 cut(s) 57, 395
Tth111I GACNNNGTC 1 cut(s) 265
XmnI GAANNNNTTC 1 cut(s) 335
XspI CTAG 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.