Rorug03G0249400

Alkaline and neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0020520
Physical Location & Seq
Reverse (-)
23471936 .. 23485141
13206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0249400.1

Sequence Viewer

Length: 2883 bp
ATGATCAAGAATCTTACCAGCCCTGTTTCTTCTATTCTCATTTTCCTTTTATTGTTTAGGAATTCCTTGGCTGCTGCCCAAAACACAATGATCCCAGTAGATGTGGGTATGATCCTTGACTTTGATTCTTTGTTTGGGAAGATTGGGCTGAGCTGCGTCAACATGGCTCTATCCGACTTCTATGCCTCTCATGCTTACTATAAAACCAGGCTGGTACTACACACAAGGGACTCCATGAGAGATGCTGTTGTTGAAGCTGCTGCAGCTCTAGACTTGATAAAAAATATTGAAGTGAAAGCTATCATAGGGGCGGAATCATCAATGAAAGCCAACTTTGTAATTGATCTTGGAGATAAAGCTAAGGTGCCTATAATCTCATTTTCGACGGCAAATCCTTCCCTGACTTCTTTTCAGCGTTCATATTTTTTCCAAGTTGCAAAGAATGACTCATCTCAAGTGAAAGCCATAAGTGCCATAATTCAATCTTTTGGCTGGAATGAAGCAGTGCCCATTTTTGTCGACGATGAGTTTTGGGAGGGTACATTACCTTATATGATTAATGTTCTACAAGAAGTCGGTGCTCGTATTCCCTACTGCAGTGTCATTCCTCTAATGGCCACTGATGACCAAATTGTTGTGAAGCTTCAACATCTCATGACAATGAAAACAAGGGTATTCATTGTACACATGTTGCCTTCTCTTGGCTCCCGGCTTTTTGCCAAGGCAAAAGAGATGGGCATGATGGGTGAAGGTTATGTTTGGATAATGACAGGTGGAATGACCAACTCTTTTGGATCCATAAATTCTTCTGTCACAGATAACATGCAAGGTGTGTTAGCATTAAGAACTTATGTTCCAAATACACAAGAGCTTGAAAATTTCAGGGTCCGATGGCACAGAAAATTCCAAATAGAGAATCCAACCATCCTTAATGTTAGATCGGATGATATTTTTGGACTGTGGGCTTATGATGCTGCTTGGGCACTAGCCATGGCAGTTGAGAAGGTTATAAATTCTACAAGCTTCAACTTTCAAAAGGTGAACATTTCTGGCAGTTCCACTGATCTAGAACGATTAGGGGTCTCTGAAAGTGGTCCTGAACTCGTCCAAGCACTATCAACGACAAAATTCAGAGGCCTTTCTGGAAATTTCAGTCTTCTTAATGGACAACTACAGTCTTCAATTTTTGAGATAATCAATGTGAATGGTAATGGGGTAAGAGGAGTTGGATATTGGACACCCAAAAATGGACTTGTTAGAAATTTAAACTCGGCAAGCAGCGGATATTCTAGTTCTACTTCCAATCCCAGTGACCTTGGACTTATTATATGGCCCGGAGACACCATCACCATTCCAAAAGGTTGGCAGATTCCTAACCATGGGAATAAGTTGAAAATTCTAGTTCCAGTGAAGCATGGGTTTAAAGAATTTGTAAATGTGACAATGGATCCTACAACTAACACAACCATGGTCACTGGATATTGCATAGACATTTTTAAAGCAGTAATCGAAGAATTACCATATGCTGTTTCTTATGAATTTTATCCCTTTGTAAAGCCTAATGGTGAACCAGCTGGCAGCTACGAGGATTTGATCTCTCAAGTGTTTCTTGGGAACTATGATGCAGCAGTTGGAGATATAACCATTAGAGAAAATAGGTCATCGTTAGTTGACTTTACATTGCCATACACAGAATCTGGAGTATCAATGGTGGTGCCTATCAAAGTGAAGAAAAGTAGGCAAAGTGCATTGGTGTTCTTGAAGCCTTTTACTTGGGACCTTTGGGTAACAAGCTGTTGCTTTTTCTTTGTCATTGGGTTCGTGATCTGGGTTCTTGAACACCGATTGAATGAAGATTTTCGTGGGCCTCCGTCACACCAGATTGGTACAACCTTTTGGTTCTCTTTCTCAACCATGGGGAGAAAGTACGTAGTGAGCAACTTAGCTAGGACTGTGGTGATCGTGTGGTGCTTTGTTGTCCTCATACTGACACAAAGTTATACTGCCAGTTTGACATCACTTTTAACAGTTCAACAGCTCCAACCAACCGTTGCTAATGTAGGTCTGCTCCTCAAGAATGGAGACTTCGTTGGCTTCCAAGAAGGTTCTTCTGTACAGAACTTTTTGACATATGAACTGGGATTTCATGTGGAAAAGCTTAGGGCATATAGGTCTGGAGAAGATCTGGCTCAACTGTTTGAGAAAGGGACAATTTCTGCTGCTTTTGATGAAACCCCTTTCATGAACCTTTTCCTTTCAACTTATTGCTCAAAATATACCATGGTTGAGCCAATACTATCCAAAACTGGCAGCGGCTTTGAATTTGCCTTTAAGAAAGGTGTGCCTCTTACACTAGATGTTTCAAGGATGATCGCTAATTTGCAAGAGGGAGGTAAAATGAAAACTGTCATGGACAAGTGGTTCAAGAAAGAAGCAAGTTGTACATACCCCAGCATAGACTCTAATTCCAACAGTCCTCTTAGCCTTGATAGCCTTTGGGGTCTTTTCCTTATTGTTGGAGTTACTTCGTCATCAGCTCTTCTGATACATGTAGCTATGGTCTTCTATCAGAATCGGCACGTCTTGATACCCTCGGATCCAGAACTCTCTCTTTGGAGGAAAATGAGTCTCATATTACAGATATTCGACCAAAGAGACCTCCCTTCGCATACTTTTTGGCGCAGGGATCAGCAAGATAATGCTCAAGCTAGAGCTTCAGTTGATTCCTCACCGAACATCATAACCAATAGCTTGCCAAGACCATCAGATTTTTCACACCACGCAAGTTCAGATATTACTGATTTTGAAATGCAGGAAACACCTTCTAGTTCTACCAATGGGGATTTGAATCCCAGTGGTCAAGCCATCCAAGAGACTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

960

Amino Acids

106.79

Weight (kDa)

6.07

Isoelectric Point (pI)

35.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 32 - 383 1.4e-14 Periplasmic binding protein
ANF_receptor PF01094 52 - 404 1.3e-79 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 483 - 572 9.6e-11 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 488 - 809 6.4e-11 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 591 - 841 6e-33 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020520)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0491441
rosa_laevigata RLG00000022841
rosa_multiflora Rmu_sc0006034.1_g000004
rosa_rugosa Rorug03G0249400
rosa_samantha Rh3AG298600 Rh3DG333100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1010
AccB1I GGYRCC 2 cut(s) 364, 1714
AccI GTMKAC 1 cut(s) 519
AciI CCGC 3 cut(s) 311, 1281, 2313
AclWI GGATC 9 cut(s) 85, 106, 789, 802, 1442, 1455, 2591, 2604, 2694
AcoI YGGCCR 1 cut(s) 615
AcuI CTGAAG 1 cut(s) 2700
AfaI GTAC 7 cut(s) 216, 541, 684, 1888, 1928, 2115, 2443
AfiI CCNNNNNNNGG 3 cut(s) 701, 1247, 1379
AflIII ACRYGT 2 cut(s) 687, 2548
AjiI CACGTC 1 cut(s) 2581
AjnI CCWGG 1 cut(s) 206
AloI GAACNNNNNNTCC 4 cut(s) 838, 870, 2405, 2437
Alw21I GWGCWC 1 cut(s) 583
Alw26I GTCTC 6 cut(s) 1087, 1332, 2076, 2633, 2649, 2867
AlwI GGATC 9 cut(s) 85, 106, 789, 802, 1442, 1455, 2591, 2604, 2694
AlwNI CAGNNNCTG 1 cut(s) 1697
AoxI GGCC 4 cut(s) 615, 1135, 1331, 1865
AseI ATTAAT 1 cut(s) 558
Asp700I GAANNNNTTC 2 cut(s) 1857, 2249
AspLEI GCGC 1 cut(s) 2682
AspS9I GGNCC 5 cut(s) 886, 1094, 1332, 1777, 1865
AsuC2I CCSGG 2 cut(s) 709, 1335
AsuHPI GGTGA 6 cut(s) 758, 1051, 1339, 1577, 1969, 2721
AvaII GGWCC 3 cut(s) 886, 1094, 1777
BaeGI GKGCMC 2 cut(s) 510, 985
BalI TGGCCA 1 cut(s) 617
BamHI GGATCC 3 cut(s) 794, 1447, 2596
BanI GGYRCC 2 cut(s) 364, 1714
BbsI GAAGAC 3 cut(s) 1148, 1170, 2554
Bbv12I GWGCWC 1 cut(s) 583
BccI CCATC 7 cut(s) 727, 736, 885, 932, 1352, 2770, 2873
BceAI ACGGC 1 cut(s) 402
BcgI CGANNNNNNTGC 2 cut(s) 164, 198
BciT130I CCWGG 1 cut(s) 208
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 2 cut(s) 709, 1335
BcoDI GTCTC 6 cut(s) 1087, 1332, 2076, 2633, 2649, 2867
BfaI CTAG 9 cut(s) 269, 986, 1067, 1290, 1400, 1947, 2354, 2709, 2826
BfmI CTRYAG 3 cut(s) 261, 595, 1172
BglII AGATCT 1 cut(s) 2182
BlpI GCTNAGC 1 cut(s) 149
Bme1390I CCNGG 3 cut(s) 208, 709, 1335
Bme18I GGWCC 3 cut(s) 886, 1094, 1777
BmgBI CACGTC 1 cut(s) 2581
BmgT120I GGNCC 5 cut(s) 886, 1094, 1332, 1777, 1865
BmiI GGNNCC 8 cut(s) 366, 706, 796, 887, 1449, 1716, 1778, 2598
BmrFI CCNGG 3 cut(s) 208, 709, 1335
BmrI ACTGGG 4 cut(s) 89, 1302, 2147, 2847
BmsI GCATC 3 cut(s) 232, 961, 1612
BmuI ACTGGG 4 cut(s) 89, 1302, 2147, 2847
BpiI GAAGAC 3 cut(s) 1148, 1170, 2554
BpmI CTGGAG 2 cut(s) 1719, 2196
Bpu10I CCTNAGC 2 cut(s) 360, 2159
Bpu1102I GCTNAGC 1 cut(s) 149
BpuEI CTTGAG 4 cut(s) 438, 1584, 2057, 2688
BpuMI CCSGG 2 cut(s) 709, 1335
BsaAI YACGTR 1 cut(s) 1930
BsaBI GATNNNNATC 1 cut(s) 2847
BsaI GGTCTC 2 cut(s) 1087, 2649
BsaJI CCNNGG 9 cut(s) 66, 720, 990, 1315, 1378, 1467, 1914, 2280, 2592
BsaXI ACNNNNNCTCC 2 cut(s) 2073, 2103
Bsc4I CCNNNNNNNGG 3 cut(s) 701, 1247, 1379
Bse1I ACTGG 8 cut(s) 95, 1308, 1406, 1480, 2007, 2142, 2311, 2853
Bse3DI GCAATG 1 cut(s) 1679
Bse8I GATNNNNATC 1 cut(s) 2847
BseBI CCWGG 1 cut(s) 208
BseDI CCNNGG 9 cut(s) 66, 720, 990, 1315, 1378, 1467, 1914, 2280, 2592
BseGI GGATG 4 cut(s) 924, 949, 2373, 2865
BseJI GATNNNNATC 1 cut(s) 2847
BseLI CCNNNNNNNGG 3 cut(s) 701, 1247, 1379
BseMI GCAATG 1 cut(s) 1679
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 8 cut(s) 95, 1308, 1406, 1480, 2007, 2142, 2311, 2853
BseRI GAGGAG 2 cut(s) 1236, 2060
BseSI GKGCMC 2 cut(s) 510, 985
BseYI CCCAGC 1 cut(s) 2450
BshFI GGCC 4 cut(s) 617, 1137, 1333, 1867
BshNI GGYRCC 2 cut(s) 364, 1714
BsiHKAI GWGCWC 1 cut(s) 583
BsiSI CCGG 2 cut(s) 709, 1335
BslFI GGGAC 3 cut(s) 242, 1790, 2221
BslI CCNNNNNNNGG 3 cut(s) 701, 1247, 1379
BsmAI GTCTC 6 cut(s) 1087, 1332, 2076, 2633, 2649, 2867
BsmFI GGGAC 3 cut(s) 242, 1790, 2221
BsnI GGCC 4 cut(s) 617, 1137, 1333, 1867
Bso31I GGTCTC 2 cut(s) 1087, 2649
Bsp1286I GDGCHC 3 cut(s) 510, 583, 985
Bsp1407I TGTACA 3 cut(s) 682, 2113, 2441
Bsp1720I GCTNAGC 1 cut(s) 149
Bsp19I CCATGG 5 cut(s) 990, 1378, 1467, 1914, 2280
BspACI CCGC 3 cut(s) 311, 1281, 2313
BspANI GGCC 4 cut(s) 617, 1137, 1333, 1867
BspCNI CTCAG 1 cut(s) 141
BspHI TCATGA 2 cut(s) 654, 2241
BspLI GGNNCC 8 cut(s) 366, 706, 796, 887, 1449, 1716, 1778, 2598
BspMAI CTGCAG 2 cut(s) 265, 599
BspPI GGATC 9 cut(s) 85, 106, 789, 802, 1442, 1455, 2591, 2604, 2694
BspQI GCTCTTC 1 cut(s) 2544
BspT107I GGYRCC 2 cut(s) 364, 1714
BspTNI GGTCTC 2 cut(s) 1087, 2649
BsrDI GCAATG 1 cut(s) 1679
BsrGI TGTACA 3 cut(s) 682, 2113, 2441
BsrI ACTGG 8 cut(s) 95, 1308, 1406, 1480, 2007, 2142, 2311, 2853
BssECI CCNNGG 9 cut(s) 66, 720, 990, 1315, 1378, 1467, 1914, 2280, 2592
BssT1I CCWWGG 8 cut(s) 66, 720, 990, 1315, 1378, 1467, 1914, 2280
Bst2UI CCWGG 1 cut(s) 208
Bst4CI ACNGT 8 cut(s) 960, 1176, 1954, 2029, 2050, 2196, 2407, 2474
Bst6I CTCTTC 1 cut(s) 2544
BstAUI TGTACA 3 cut(s) 682, 2113, 2441
BstBAI YACGTR 1 cut(s) 1930
BstC8I GCNNGC 3 cut(s) 1276, 1576, 2753
BstDEI CTNAG 5 cut(s) 149, 360, 1942, 2159, 2480
BstDSI CCRYGG 5 cut(s) 990, 1378, 1467, 1914, 2280
BstF5I GGATG 4 cut(s) 924, 949, 2373, 2865
BstHHI GCGC 1 cut(s) 2682
BstMAI GTCTC 6 cut(s) 1087, 1332, 2076, 2633, 2649, 2867
BstMWI GCNNNNNNNGC 6 cut(s) 191, 263, 470, 971, 980, 2490
BstNI CCWGG 1 cut(s) 208
BstNSI RCATGY 3 cut(s) 691, 826, 2552
BstSCI CCNGG 3 cut(s) 206, 707, 1333
BstSFI CTRYAG 3 cut(s) 261, 595, 1172
BstSLI GKGCMC 2 cut(s) 510, 985
BstSNI TACGTA 1 cut(s) 1930
BstV2I GAAGAC 3 cut(s) 1148, 1170, 2554
BstX2I RGATCY 4 cut(s) 794, 1447, 2182, 2596
BstXI CCANNNNNNTGG 1 cut(s) 1362
BstYI RGATCY 4 cut(s) 794, 1447, 2182, 2596
BsuRI GGCC 4 cut(s) 617, 1137, 1333, 1867
BtgI CCRYGG 5 cut(s) 990, 1378, 1467, 1914, 2280
BtrI CACGTC 1 cut(s) 2581
BtsCI GGATG 4 cut(s) 924, 949, 2373, 2865
BtsI GCAGTG 2 cut(s) 510, 604
BtsIMutI CAGTG 8 cut(s) 510, 604, 618, 1059, 1315, 1413, 1473, 2860
Cac8I GCNNGC 3 cut(s) 1276, 1576, 2753
CaiI CAGNNNCTG 1 cut(s) 1697
CciI TCATGA 2 cut(s) 654, 2241
CfoI GCGC 1 cut(s) 2682
Cfr13I GGNCC 5 cut(s) 886, 1094, 1332, 1777, 1865
CseI GACGC 1 cut(s) 145
Csp6I GTAC 7 cut(s) 215, 540, 683, 1887, 1927, 2114, 2442
CviQI GTAC 7 cut(s) 215, 540, 683, 1887, 1927, 2114, 2442
DdeI CTNAG 5 cut(s) 149, 360, 1942, 2159, 2480
DraI TTTAAA 3 cut(s) 1266, 1423, 1498
EaeI YGGCCR 1 cut(s) 615
Eam1104I CTCTTC 1 cut(s) 2544
EarI CTCTTC 1 cut(s) 2544
EciI GGCGGA 1 cut(s) 326
Eco105I TACGTA 1 cut(s) 1930
Eco130I CCWWGG 8 cut(s) 66, 720, 990, 1315, 1378, 1467, 1914, 2280
Eco147I AGGCCT 1 cut(s) 1137
Eco31I GGTCTC 2 cut(s) 1087, 2649
Eco47I GGWCC 3 cut(s) 886, 1094, 1777
Eco57I CTGAAG 1 cut(s) 2700
EcoO109I RGGNCCY 1 cut(s) 1777
EcoRI GAATTC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 206
EcoT14I CCWWGG 8 cut(s) 66, 720, 990, 1315, 1378, 1467, 1914, 2280
ErhI CCWWGG 8 cut(s) 66, 720, 990, 1315, 1378, 1467, 1914, 2280
FalI AAGNNNNNCTT 2 cut(s) 1593, 1625
FaqI GGGAC 3 cut(s) 242, 1790, 2221
FauNDI CATATG 2 cut(s) 1522, 2131
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 519
FokI GGATG 4 cut(s) 911, 956, 2380, 2852
FspBI CTAG 9 cut(s) 269, 986, 1067, 1290, 1400, 1947, 2354, 2709, 2826
GlaI GCGC 1 cut(s) 2681
GsaI CCCAGC 1 cut(s) 2454
GsuI CTGGAG 2 cut(s) 1719, 2196
HaeIII GGCC 4 cut(s) 617, 1137, 1333, 1867
HapII CCGG 2 cut(s) 709, 1335
HgaI GACGC 1 cut(s) 145
HhaI GCGC 1 cut(s) 2682
Hin6I GCGC 1 cut(s) 2680
HinP1I GCGC 1 cut(s) 2680
HincII GTYRAC 3 cut(s) 160, 520, 1672
HindII GTYRAC 3 cut(s) 160, 520, 1672
HindIII AAGCTT 3 cut(s) 641, 1021, 2156
HpaII CCGG 2 cut(s) 709, 1335
HphI GGTGA 6 cut(s) 758, 1051, 1339, 1577, 1969, 2721
Hpy166II GTNNAC 6 cut(s) 160, 520, 685, 1042, 1568, 1672
Hpy8I GTNNAC 6 cut(s) 160, 520, 685, 1042, 1568, 1672
Hpy99I CGWCG 2 cut(s) 388, 524
HpyAV CCTTC 7 cut(s) 405, 705, 743, 997, 2096, 2673, 2832
HpyCH4III ACNGT 8 cut(s) 960, 1176, 1954, 2029, 2050, 2196, 2407, 2474
HpyCH4IV ACGT 2 cut(s) 1929, 2580
HpyCH4V TGCA 9 cut(s) 263, 437, 597, 826, 1485, 1625, 1748, 2383, 2812
HpyF10VI GCNNNNNNNGC 6 cut(s) 191, 263, 470, 971, 980, 2490
HpyF3I CTNAG 5 cut(s) 149, 360, 1942, 2159, 2480
HpySE526I ACGT 2 cut(s) 1929, 2580
HspAI GCGC 1 cut(s) 2680
Ksp22I TGATCA 1 cut(s) 3
LguI GCTCTTC 1 cut(s) 2544
LmnI GCTCC 3 cut(s) 710, 2043, 2073
LweI GCATC 3 cut(s) 232, 961, 1612
MaeI CTAG 9 cut(s) 269, 986, 1067, 1290, 1400, 1947, 2354, 2709, 2826
MaeII ACGT 2 cut(s) 1929, 2580
MaeIII GTNAC 7 cut(s) 811, 1310, 1438, 1471, 1786, 1872, 2521
MflI RGATCY 4 cut(s) 794, 1447, 2182, 2596
MhlI GDGCHC 3 cut(s) 510, 583, 985
MlsI TGGCCA 1 cut(s) 617
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 5 cut(s) 224, 440, 2453, 2635, 2869
MmeI TCCRAC 7 cut(s) 198, 944, 1207, 1612, 2065, 2493, 2497
Mox20I TGGCCA 1 cut(s) 617
MroXI GAANNNNTTC 2 cut(s) 1857, 2249
MscI TGGCCA 1 cut(s) 617
MseI TTAA 9 cut(s) 558, 842, 930, 1161, 1265, 1422, 1497, 2024, 2331
MslI CAYNNNNRTG 2 cut(s) 659, 1466
Msp20I TGGCCA 1 cut(s) 617
MspA1I CMGCKG 3 cut(s) 1281, 1574, 2313
MspI CCGG 2 cut(s) 709, 1335
MspR9I CCNGG 3 cut(s) 208, 709, 1335
MvaI CCWGG 1 cut(s) 208
MwoI GCNNNNNNNGC 6 cut(s) 191, 263, 470, 971, 980, 2490
NciI CCSGG 2 cut(s) 709, 1335
NcoI CCATGG 5 cut(s) 990, 1378, 1467, 1914, 2280
NdeI CATATG 2 cut(s) 1522, 2131
NlaIV GGNNCC 8 cut(s) 366, 706, 796, 887, 1449, 1716, 1778, 2598
NmeAIII GCCGAG 1 cut(s) 1250
NmuCI GTSAC 5 cut(s) 811, 1310, 1438, 1471, 1872
NspI RCATGY 3 cut(s) 691, 826, 2552
PagI TCATGA 2 cut(s) 654, 2241
PceI AGGCCT 1 cut(s) 1137
PciI ACATGT 2 cut(s) 687, 2548
PciSI GCTCTTC 1 cut(s) 2544
PdmI GAANNNNTTC 2 cut(s) 1857, 2249
PfeI GAWTC 9 cut(s) 10, 125, 314, 916, 1369, 1694, 2572, 2723, 2848
PleI GAGTC 5 cut(s) 224, 440, 2453, 2634, 2869
PpsI GAGTC 5 cut(s) 224, 440, 2453, 2634, 2869
Ppu21I YACGTR 1 cut(s) 1930
PpuMI RGGWCCY 1 cut(s) 1777
PscI ACATGT 2 cut(s) 687, 2548
PshBI ATTAAT 1 cut(s) 558
PsiI TTATAA 1 cut(s) 1010
Psp5II RGGWCCY 1 cut(s) 1777
Psp6I CCWGG 1 cut(s) 206
PspFI CCCAGC 1 cut(s) 2450
PspGI CCWGG 1 cut(s) 206
PspN4I GGNNCC 8 cut(s) 366, 706, 796, 887, 1449, 1716, 1778, 2598
PspPI GGNCC 5 cut(s) 886, 1094, 1332, 1777, 1865
PspPPI RGGWCCY 1 cut(s) 1777
PstI CTGCAG 2 cut(s) 265, 599
PstNI CAGNNNCTG 1 cut(s) 1697
PsuI RGATCY 4 cut(s) 794, 1447, 2182, 2596
PvuII CAGCTG 1 cut(s) 1574
RsaI GTAC 7 cut(s) 216, 541, 684, 1888, 1928, 2115, 2443
RsaNI GTAC 7 cut(s) 215, 540, 683, 1887, 1927, 2114, 2442
RseI CAYNNNNRTG 2 cut(s) 659, 1466
SalI GTCGAC 1 cut(s) 518
SapI GCTCTTC 1 cut(s) 2544
SaqAI TTAA 9 cut(s) 558, 842, 930, 1161, 1265, 1422, 1497, 2024, 2331
Sau96I GGNCC 5 cut(s) 886, 1094, 1332, 1777, 1865
SchI GAGTC 5 cut(s) 224, 440, 2453, 2635, 2869
ScrFI CCNGG 3 cut(s) 208, 709, 1335
SduI GDGCHC 3 cut(s) 510, 583, 985
SfaNI GCATC 3 cut(s) 232, 961, 1612
SfcI CTRYAG 3 cut(s) 261, 595, 1172
SinI GGWCC 3 cut(s) 886, 1094, 1777
SmiMI CAYNNNNRTG 2 cut(s) 659, 1466
SmlI CTYRAG 4 cut(s) 453, 1599, 2072, 2703
SmoI CTYRAG 4 cut(s) 453, 1599, 2072, 2703
SnaBI TACGTA 1 cut(s) 1930
SseBI AGGCCT 1 cut(s) 1137
SsiI CCGC 3 cut(s) 311, 1281, 2313
SspI AATATT 1 cut(s) 286
SspMI CTAG 9 cut(s) 269, 986, 1067, 1290, 1400, 1947, 2354, 2709, 2826
StuI AGGCCT 1 cut(s) 1137
StyD4I CCNGG 3 cut(s) 206, 707, 1333
StyI CCWWGG 8 cut(s) 66, 720, 990, 1315, 1378, 1467, 1914, 2280
TaaI ACNGT 8 cut(s) 960, 1176, 1954, 2029, 2050, 2196, 2407, 2474
TaiI ACGT 2 cut(s) 1932, 2583
TaqI TCGA 5 cut(s) 383, 519, 1509, 2646, 2878
TatI WGTACW 3 cut(s) 682, 2113, 2441
TauI GCSGC 1 cut(s) 2316
TfiI GAWTC 9 cut(s) 10, 125, 314, 916, 1369, 1694, 2572, 2723, 2848
Tru1I TTAA 9 cut(s) 558, 842, 930, 1161, 1265, 1422, 1497, 2024, 2331
Tru9I TTAA 9 cut(s) 558, 842, 930, 1161, 1265, 1422, 1497, 2024, 2331
TscAI CASTG 8 cut(s) 510, 604, 625, 1066, 1315, 1413, 1480, 2860
TseFI GTSAC 5 cut(s) 811, 1310, 1438, 1471, 1872
Tsp45I GTSAC 5 cut(s) 811, 1310, 1438, 1471, 1872
TspGWI ACGGA 1 cut(s) 1860
TspRI CASTG 8 cut(s) 510, 604, 625, 1066, 1315, 1413, 1480, 2860
VpaK11BI GGWCC 3 cut(s) 886, 1094, 1777
VspI ATTAAT 1 cut(s) 558
XbaI TCTAGA 2 cut(s) 268, 1066
XceI RCATGY 3 cut(s) 691, 826, 2552
XmiI GTMKAC 1 cut(s) 519
XmnI GAANNNNTTC 2 cut(s) 1857, 2249
XspI CTAG 9 cut(s) 269, 986, 1067, 1290, 1400, 1947, 2354, 2709, 2826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.