RchiOBHm_Chr4g0388931

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0014252
Physical Location & Seq
Forward (+)
4117059 .. 4120472
3414 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36210

Sequence Viewer

Length: 1401 bp
ATGCAGAAGAGGGAGCTAAACAAGTCCGGTGGATCTTCCGGCGGAGCCTCCGCACCGCCGGCGAAGCGAGGCCGTCCATTCGGCAGCGGCCACGGTAGTGCCGCAGCGGCCGCTGCCGCCGCCGCTGCCGCCGCAGCAGCCGATAACACCGCAGCTCCGTCGACTCTCCTCGGTCCTTCTCTCCTCGTTCACAATGCCTTCACCGCGGAAAACAATAAGAGGATAGTTCATGCTCTTCAGAGCGGACTGAAGAGTGAGCTGACATGGGCGTTGAATACTCTCACAATACTCTCTTTCAAAGAAAAAGATGATTTTCGCAAGGATAGCAATAATGCTCTCACGAGGATTTCCGGCTTGCTTGATGCTCTCCTCACTGTTATTGATGATTGGCGCGATATAGCGCTTCCTAAGGAACATGTAAGGGCGCCAAGAGTGAGGAATTTAGGTGCGAATTTGGTTGTAAGTGGGTTCGGGCATGAGTACGAGGTGTTGGGGTCTAATAGCACTGTTCCGCATCCCAGCTTAGGATCTGGTTCCGGAGAAGCATCTATGCTGAGTAATGTGAGCAATCTTCGTTCTTCGGAGTGGTGGCTTGATGAAGATGGTCTATTTAATCTTGATGATGAAGGTAGAGCGGAAAGACAGCAATGTGCTGTTGCGGCTTCTAATATCATCCGCAACTTCTCTTTCATGCAAGACAATGAGAATATTATGGCTGTCCATCGCCATTGTCTGGAAACAGTCTTTCAGTGCATAGAAGATTACATCACAGAAGATGAAGAACTTGTGACAAATGCTCTCGAAACAATTGTAAATTTGTCTCCGATGCTGGATCTTCAAATTTTTGGTTCATCAAAGGCATCCTACATCAAGATGACAGCAAAACGTGCAGTTCAAGCCATTGTGGGTGTGCTGGAGTCTCCGGTCAAAGCTTGGCATTGCGCTGCAGCTGAGTTACTTGGGCGTTTGGTCATAAATCCTGATAATGAACCTTTTCTTCTTCCCTTCATTCCGCAGATACACAAACGGTTAGTTGATCTTATGAGCTTACCAGCAATAGATGCACAAGCAGCTGCTGTTGGTGCGCTCTATAACTTCTCTGAAGTAAATATGGACTGCAGACTGAAACTTGCTAACGAGCGATGGGCAATTGATCGACTGCTAAAAGTTATTAAGGGGCCCCATCCTGTTCCCGAAATTTGCAGGAAAGCTGCTATGATATTAGAAAGCCTCGTGTCCGAACCACAGAACAGAGGATTGCTGCTAGCTTATGAGAATGCATTTGCAGAAGTACTATTCTCAGATGCCAAATATTCCGACATCTTTGCCAGGATTTTGTATGAACTGACGTCTAGACCAAACAACAAAATGGCAGCAGCCCGTGGTATTTGGGGCATGTAA

Protein Analysis

466

Amino Acids

50.67

Weight (kDa)

5.93

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014252)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22990
fragaria_vesca FvH4_4g01960
malus_domestica MD13G1203000.v1.1 MD16G1218200.v1.1
prunus_persica Prupe.1G025500_v2.0.a1
pyrus_communis pycom13g17630 pycom16g17250
rosa_chinensis RchiOBHm_Chr4g0388931
rosa_laevigata RLG00000010006
rosa_multiflora Rmu_sc0005486.1_g000003
rosa_roxburghii Rroxscaffold_5G00335250
rosa_rugosa Rorug03G0321400 Rorug03G0321500
rosa_samantha Rh4AG025800 Rh4BG019100 Rh4DG019600
rosa_wichuraiana Rw4G001860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1352
AccB1I GGYRCC 1 cut(s) 424
AccB7I CCANNNNNTGG 1 cut(s) 733
AccBSI CCGCTC 2 cut(s) 243, 635
AccI GTMKAC 1 cut(s) 161
AccII CGCG 2 cut(s) 206, 393
AccIII TCCGGA 1 cut(s) 536
AclWI GGATC 3 cut(s) 40, 535, 840
AcoI YGGCCR 2 cut(s) 88, 108
AcsI RAATTY 5 cut(s) 439, 451, 814, 840, 1197
AcuI CTGAAG 3 cut(s) 221, 269, 1122
AcyI GRCGYC 2 cut(s) 425, 1349
AfaI GTAC 2 cut(s) 482, 1293
AfeI AGCGCT 1 cut(s) 402
AfiI CCNNNNNNNGG 2 cut(s) 524, 733
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 4 cut(s) 274, 298, 839, 896
AjnI CCWGG 1 cut(s) 1328
AleI CACNNNNGTG 1 cut(s) 96
Alw26I GTCTC 2 cut(s) 825, 924
AlwI GGATC 3 cut(s) 40, 535, 840
AlwNI CAGNNNCTG 1 cut(s) 1076
Aor13HI TCCGGA 1 cut(s) 536
Aor51HI AGCGCT 1 cut(s) 402
AoxI GGCC 4 cut(s) 70, 88, 108, 1178
ApaI GGGCCC 1 cut(s) 1182
ApoI RAATTY 5 cut(s) 439, 451, 814, 840, 1197
Asp700I GAANNNNTTC 1 cut(s) 993
AspLEI GCGC 5 cut(s) 393, 403, 427, 944, 1087
AspS9I GGNCC 3 cut(s) 173, 1178, 1179
AsuHPI GGTGA 1 cut(s) 193
AsuNHI GCTAGC 1 cut(s) 1264
AvaII GGWCC 1 cut(s) 173
AxyI CCTNAGG 1 cut(s) 408
BaeGI GKGCMC 1 cut(s) 1182
BanI GGYRCC 1 cut(s) 424
BanII GRGCYC 1 cut(s) 1182
BauI CACGAG 2 cut(s) 340, 1232
BccI CCATC 4 cut(s) 596, 729, 1137, 1191
BceAI ACGGC 1 cut(s) 57
BcgI CGANNNNNNTGC 4 cut(s) 141, 175, 1307, 1341
BciT130I CCWGG 1 cut(s) 1330
BcoDI GTCTC 2 cut(s) 825, 924
BfaI CTAG 2 cut(s) 1265, 1353
BfmI CTRYAG 2 cut(s) 945, 1117
BfoI RGCGCY 2 cut(s) 404, 428
BglI GCCNNNNNGGC 1 cut(s) 107
BmcAI AGTACT 1 cut(s) 1293
Bme1390I CCNGG 1 cut(s) 1330
Bme18I GGWCC 1 cut(s) 173
BmgT120I GGNCC 3 cut(s) 173, 1178, 1179
BmiI GGNNCC 6 cut(s) 46, 426, 535, 1179, 1180, 1181
BmrFI CCNGG 1 cut(s) 1330
BmsI GCATC 7 cut(s) 352, 523, 554, 816, 869, 1051, 1294
BmtI GCTAGC 1 cut(s) 1268
BpmI CTGGAG 1 cut(s) 935
Bpu10I CCTNAGC 1 cut(s) 523
BsaHI GRCGYC 2 cut(s) 425, 1349
BsaJI CCNNGG 4 cut(s) 91, 169, 204, 1381
BsaWI WCCGGW 3 cut(s) 26, 536, 922
BsaXI ACNNNNNCTCC 2 cut(s) 139, 169
Bsc4I CCNNNNNNNGG 2 cut(s) 524, 733
Bse118I RCCGGY 1 cut(s) 58
Bse21I CCTNAGG 1 cut(s) 408
Bse3DI GCAATG 2 cut(s) 653, 937
BseAI TCCGGA 1 cut(s) 536
BseBI CCWGG 1 cut(s) 1330
BseDI CCNNGG 4 cut(s) 91, 169, 204, 1381
BseGI GGATG 4 cut(s) 514, 672, 860, 1183
BseLI CCNNNNNNNGG 2 cut(s) 524, 733
BseMI GCAATG 2 cut(s) 653, 937
BseMII CTCAG 3 cut(s) 545, 942, 1314
BseRI GAGGAG 3 cut(s) 158, 173, 359
BseSI GKGCMC 1 cut(s) 1182
BseX3I CGGCCG 1 cut(s) 108
BseYI CCCAGC 1 cut(s) 518
BsgI GTGCAG 1 cut(s) 909
Bsh1236I CGCG 2 cut(s) 206, 393
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 4 cut(s) 72, 90, 110, 1180
BshNI GGYRCC 1 cut(s) 424
BsiEI CGRYCG 1 cut(s) 111
BsiSI CCGG 6 cut(s) 27, 39, 59, 351, 537, 923
BslI CCNNNNNNNGG 2 cut(s) 524, 733
BsmAI GTCTC 2 cut(s) 825, 924
BsmI GAATGC 1 cut(s) 1282
BsnI GGCC 4 cut(s) 72, 90, 110, 1180
Bsp120I GGGCCC 1 cut(s) 1178
Bsp1286I GDGCHC 1 cut(s) 1182
Bsp13I TCCGGA 1 cut(s) 536
Bsp143I GATC 5 cut(s) 32, 527, 832, 1036, 1153
BspANI GGCC 4 cut(s) 72, 90, 110, 1180
BspCNI CTCAG 3 cut(s) 546, 943, 1313
BspEI TCCGGA 1 cut(s) 536
BspFNI CGCG 2 cut(s) 206, 393
BspLI GGNNCC 6 cut(s) 46, 426, 535, 1179, 1180, 1181
BspMAI CTGCAG 2 cut(s) 949, 1121
BspOI GCTAGC 1 cut(s) 1268
BspPI GGATC 3 cut(s) 40, 535, 840
BspQI GCTCTTC 1 cut(s) 240
BspT107I GGYRCC 1 cut(s) 424
BsrBI CCGCTC 2 cut(s) 243, 635
BsrDI GCAATG 2 cut(s) 653, 937
BsrFI RCCGGY 1 cut(s) 58
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 4 cut(s) 91, 169, 204, 1381
BssMI GATC 5 cut(s) 32, 527, 832, 1036, 1153
BssNI GRCGYC 2 cut(s) 425, 1349
BssSI CACGAG 2 cut(s) 340, 1232
Bst2BI CACGAG 2 cut(s) 340, 1232
Bst2UI CCWGG 1 cut(s) 1330
Bst4CI ACNGT 5 cut(s) 95, 376, 508, 742, 1029
Bst6I CTCTTC 3 cut(s) 2, 240, 245
BstACI GRCGYC 2 cut(s) 425, 1349
BstAPI GCANNNNNTGC 2 cut(s) 887, 1061
BstC8I GCNNGC 3 cut(s) 60, 356, 1266
BstDEI CTNAG 5 cut(s) 408, 523, 554, 951, 1300
BstDSI CCRYGG 3 cut(s) 91, 204, 1381
BstF5I GGATG 4 cut(s) 514, 672, 860, 1183
BstFNI CGCG 2 cut(s) 206, 393
BstH2I RGCGCY 2 cut(s) 404, 428
BstHHI GCGC 5 cut(s) 393, 403, 427, 944, 1087
BstKTI GATC 5 cut(s) 35, 530, 835, 1039, 1156
BstMAI GTCTC 2 cut(s) 825, 924
BstMBI GATC 5 cut(s) 32, 527, 832, 1036, 1153
BstMCI CGRYCG 1 cut(s) 111
BstNI CCWGG 1 cut(s) 1330
BstNSI RCATGY 2 cut(s) 419, 1399
BstSCI CCNGG 1 cut(s) 1328
BstSFI CTRYAG 2 cut(s) 945, 1117
BstSLI GKGCMC 1 cut(s) 1182
BstUI CGCG 2 cut(s) 206, 393
BstX2I RGATCY 3 cut(s) 32, 527, 832
BstYI RGATCY 3 cut(s) 32, 527, 832
BstZI CGGCCG 1 cut(s) 108
Bsu36I CCTNAGG 1 cut(s) 408
BsuRI GGCC 4 cut(s) 72, 90, 110, 1180
BtgI CCRYGG 3 cut(s) 91, 204, 1381
BtgZI GCGATG 2 cut(s) 707, 1156
BtsCI GGATG 4 cut(s) 514, 672, 860, 1183
BtsIMutI CAGTG 3 cut(s) 372, 504, 755
Cac8I GCNNGC 3 cut(s) 60, 356, 1266
CaiI CAGNNNCTG 1 cut(s) 1076
CciNI GCGGCCGC 1 cut(s) 108
CfoI GCGC 5 cut(s) 393, 403, 427, 944, 1087
Cfr10I RCCGGY 1 cut(s) 58
Cfr13I GGNCC 3 cut(s) 173, 1178, 1179
Cfr42I CCGCGG 1 cut(s) 207
Csp6I GTAC 2 cut(s) 481, 1292
CspCI CAANNNNNGTGG 2 cut(s) 10, 45
CviAII CATG 6 cut(s) 230, 264, 416, 476, 691, 1396
CviQI GTAC 2 cut(s) 481, 1292
DdeI CTNAG 5 cut(s) 408, 523, 554, 951, 1300
DinI GGCGCC 1 cut(s) 426
DpnI GATC 5 cut(s) 34, 529, 834, 1038, 1155
DpnII GATC 5 cut(s) 32, 527, 832, 1036, 1153
EaeI YGGCCR 2 cut(s) 88, 108
EagI CGGCCG 1 cut(s) 108
Eam1104I CTCTTC 3 cut(s) 2, 240, 245
EarI CTCTTC 3 cut(s) 2, 240, 245
EciI GGCGGA 1 cut(s) 57
EclXI CGGCCG 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 1182
Eco47I GGWCC 1 cut(s) 173
Eco47III AGCGCT 1 cut(s) 402
Eco52I CGGCCG 1 cut(s) 108
Eco57I CTGAAG 3 cut(s) 221, 269, 1122
Eco81I CCTNAGG 1 cut(s) 408
EcoO109I RGGNCCY 2 cut(s) 1178, 1179
EcoRII CCWGG 1 cut(s) 1328
EcoT22I ATGCAT 1 cut(s) 1282
EcoT38I GRGCYC 1 cut(s) 1182
EgeI GGCGCC 1 cut(s) 426
EheI GGCGCC 1 cut(s) 426
FaeI CATG 6 cut(s) 233, 267, 419, 479, 694, 1399
FatI CATG 6 cut(s) 229, 263, 415, 475, 690, 1395
FblI GTMKAC 1 cut(s) 161
FokI GGATG 4 cut(s) 501, 659, 847, 1170
FriOI GRGCYC 1 cut(s) 1182
FspBI CTAG 2 cut(s) 1265, 1353
GlaI GCGC 5 cut(s) 392, 402, 426, 943, 1086
GsaI CCCAGC 1 cut(s) 522
GsuI CTGGAG 1 cut(s) 935
HaeII RGCGCY 2 cut(s) 404, 428
HaeIII GGCC 4 cut(s) 72, 90, 110, 1180
HapII CCGG 6 cut(s) 27, 39, 59, 351, 537, 923
HhaI GCGC 5 cut(s) 393, 403, 427, 944, 1087
Hin1I GRCGYC 2 cut(s) 425, 1349
Hin1II CATG 6 cut(s) 233, 267, 419, 479, 694, 1399
Hin6I GCGC 5 cut(s) 391, 401, 425, 942, 1085
HinP1I GCGC 5 cut(s) 391, 401, 425, 942, 1085
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HindIII AAGCTT 1 cut(s) 930
HinfI GANTC 2 cut(s) 163, 917
HpaII CCGG 6 cut(s) 27, 39, 59, 351, 537, 923
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 2 cut(s) 162, 190
Hpy188I TCNGA 7 cut(s) 240, 583, 825, 1102, 1240, 1303, 1318
Hpy188III TCNNGA 9 cut(s) 340, 537, 617, 734, 800, 871, 980, 1193, 1353
Hpy8I GTNNAC 2 cut(s) 162, 190
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 4 cut(s) 186, 208, 620, 1015
HpyCH4III ACNGT 5 cut(s) 95, 376, 508, 742, 1029
HpyCH4IV ACGT 2 cut(s) 886, 1349
HpyF3I CTNAG 5 cut(s) 408, 523, 554, 951, 1300
HpySE526I ACGT 2 cut(s) 886, 1349
Hsp92I GRCGYC 2 cut(s) 425, 1349
Hsp92II CATG 6 cut(s) 233, 267, 419, 479, 694, 1399
HspAI GCGC 5 cut(s) 391, 401, 425, 942, 1085
KasI GGCGCC 1 cut(s) 424
Kpn2I TCCGGA 1 cut(s) 536
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
KspI CCGCGG 1 cut(s) 207
Kzo9I GATC 5 cut(s) 32, 527, 832, 1036, 1153
LguI GCTCTTC 1 cut(s) 240
LmnI GCTCC 3 cut(s) 13, 44, 160
LweI GCATC 7 cut(s) 352, 523, 554, 816, 869, 1051, 1294
MaeI CTAG 2 cut(s) 1265, 1353
MaeII ACGT 2 cut(s) 886, 1349
MaeIII GTNAC 2 cut(s) 787, 954
MalI GATC 5 cut(s) 34, 529, 834, 1038, 1155
MbiI CCGCTC 2 cut(s) 243, 635
MboI GATC 5 cut(s) 32, 527, 832, 1036, 1153
MfeI CAATTG 2 cut(s) 807, 1149
MflI RGATCY 3 cut(s) 32, 527, 832
MhlI GDGCHC 1 cut(s) 1182
MluCI AATT 7 cut(s) 439, 451, 807, 814, 840, 1149, 1197
Mly113I GGCGCC 1 cut(s) 425
MlyI GAGTC 2 cut(s) 157, 926
MmeI TCCRAC 1 cut(s) 1341
Mph1103I ATGCAT 1 cut(s) 1282
MreI CGCCGGCG 1 cut(s) 58
MroI TCCGGA 1 cut(s) 536
MroNI GCCGGC 1 cut(s) 58
MroXI GAANNNNTTC 1 cut(s) 993
MseI TTAA 2 cut(s) 612, 1173
MslI CAYNNNNRTG 2 cut(s) 96, 872
MspA1I CMGCKG 7 cut(s) 87, 107, 113, 125, 206, 950, 1073
MspI CCGG 6 cut(s) 27, 39, 59, 351, 537, 923
MspR9I CCNGG 1 cut(s) 1330
MunI CAATTG 2 cut(s) 807, 1149
Mva1269I GAATGC 1 cut(s) 1282
MvaI CCWGG 1 cut(s) 1330
MvnI CGCG 2 cut(s) 206, 393
NaeI GCCGGC 1 cut(s) 60
NarI GGCGCC 1 cut(s) 425
NdeII GATC 5 cut(s) 32, 527, 832, 1036, 1153
NgoMIV GCCGGC 1 cut(s) 58
NheI GCTAGC 1 cut(s) 1264
NlaIII CATG 6 cut(s) 233, 267, 419, 479, 694, 1399
NlaIV GGNNCC 6 cut(s) 46, 426, 535, 1179, 1180, 1181
NmuCI GTSAC 1 cut(s) 787
NotI GCGGCCGC 1 cut(s) 108
NsiI ATGCAT 1 cut(s) 1282
NspI RCATGY 2 cut(s) 419, 1399
OliI CACNNNNGTG 1 cut(s) 96
PciI ACATGT 1 cut(s) 415
PciSI GCTCTTC 1 cut(s) 240
PctI GAATGC 1 cut(s) 1282
PdiI GCCGGC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 993
PflMI CCANNNNNTGG 1 cut(s) 733
PleI GAGTC 2 cut(s) 157, 925
PluTI GGCGCC 1 cut(s) 428
PpsI GAGTC 2 cut(s) 157, 925
PscI ACATGT 1 cut(s) 415
Psp6I CCWGG 1 cut(s) 1328
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 1 cut(s) 1328
PspN4I GGNNCC 6 cut(s) 46, 426, 535, 1179, 1180, 1181
PspOMI GGGCCC 1 cut(s) 1178
PspPI GGNCC 3 cut(s) 173, 1178, 1179
PstI CTGCAG 2 cut(s) 949, 1121
PstNI CAGNNNCTG 1 cut(s) 1076
PsuI RGATCY 3 cut(s) 32, 527, 832
PvuII CAGCTG 2 cut(s) 950, 1073
RsaI GTAC 2 cut(s) 482, 1293
RsaNI GTAC 2 cut(s) 481, 1292
RseI CAYNNNNRTG 2 cut(s) 96, 872
SacII CCGCGG 1 cut(s) 207
SalI GTCGAC 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 240
SaqAI TTAA 2 cut(s) 612, 1173
Sau3AI GATC 5 cut(s) 32, 527, 832, 1036, 1153
Sau96I GGNCC 3 cut(s) 173, 1178, 1179
ScaI AGTACT 1 cut(s) 1293
SchI GAGTC 2 cut(s) 157, 926
ScrFI CCNGG 1 cut(s) 1330
SduI GDGCHC 1 cut(s) 1182
SfaNI GCATC 7 cut(s) 352, 523, 554, 816, 869, 1051, 1294
SfcI CTRYAG 2 cut(s) 945, 1117
SfoI GGCGCC 1 cut(s) 426
Sfr303I CCGCGG 1 cut(s) 207
SgrAI CRCCGGYG 1 cut(s) 58
SgrBI CCGCGG 1 cut(s) 207
SinI GGWCC 1 cut(s) 173
SmiMI CAYNNNNRTG 2 cut(s) 96, 872
Sse9I AATT 7 cut(s) 439, 451, 807, 814, 840, 1149, 1197
SspDI GGCGCC 1 cut(s) 424
SspI AATATT 2 cut(s) 709, 1313
SspMI CTAG 2 cut(s) 1265, 1353
StyD4I CCNGG 1 cut(s) 1328
TaaI ACNGT 5 cut(s) 95, 376, 508, 742, 1029
TaiI ACGT 2 cut(s) 889, 1352
TaqI TCGA 3 cut(s) 161, 801, 1156
TaqII GACCGA 1 cut(s) 161
TasI AATT 7 cut(s) 439, 451, 807, 814, 840, 1149, 1197
TatI WGTACW 1 cut(s) 1291
Tru1I TTAA 2 cut(s) 612, 1173
Tru9I TTAA 2 cut(s) 612, 1173
TscAI CASTG 3 cut(s) 379, 511, 755
TseFI GTSAC 1 cut(s) 787
Tsp45I GTSAC 1 cut(s) 787
TspDTI ATGAA 9 cut(s) 218, 612, 639, 679, 792, 840, 997, 1002, 1356
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 3 cut(s) 379, 511, 755
Van91I CCANNNNNTGG 1 cut(s) 733
VpaK11BI GGWCC 1 cut(s) 173
XapI RAATTY 5 cut(s) 439, 451, 814, 840, 1197
XbaI TCTAGA 1 cut(s) 1352
XceI RCATGY 2 cut(s) 419, 1399
XmiI GTMKAC 1 cut(s) 161
XmnI GAANNNNTTC 1 cut(s) 993
XspI CTAG 2 cut(s) 1265, 1353
ZraI GACGTC 1 cut(s) 1350
ZrmI AGTACT 1 cut(s) 1293
Zsp2I ATGCAT 1 cut(s) 1282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.