RchiOBHm_Chr4g0436871
TCP Family

Belongs to the chaperonin (HSP60) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009003
Physical Location & Seq
Reverse (-)
60050714 .. 60054240
3527 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40517

Sequence Viewer

Length: 1572 bp
ATGGCATTTTCTCCAACTCCCATCTCTGCACTTTCTTCCACCAACCCAACACTGCCCAAAAGGACTTCTTCACCTTTTACCACTGCAAATCCAATAGGCATACCCAAAGACCTCTACTTTAACCATGATGGTTCTGCCACAAAGAAGCTTCTGGCAGGGGTGAGCATGGTGGCAGAGCTGGTTGGGGTTACATTGGGTCCAAAGGGAAGGAATGTGGTGCTGCAGAATAAGTATGGACCTCCCAAGATTGTCAATGATGGTGAAACTGTTCTCAAAGAGATCGAGTTGGAGGACTCTGTGGAGAATGTCGGAGTTAAACTGTTAAGGCAAGCTGGAGCAAAAACAAATGACCTGGCCGGAGATGGTTCCACTACAGCTGTAATTCTTGCTCACGGTTTAATTACTGAAGGTGTGAAGGTTACTGCAGCTGGCATGAATCCCATTCAAATTGCTCGTGGGATTGAGAAGACTGCAGTGGCCCTAGTTTCTGAACTCAAATTGATGTCCAGAGAGATTGAGGATCATGAACTTGCAGATGTTGCTGCAGTTAGTGCAGGCAATGATTATACAGTGGGGAACATGATTTACAATGCTCTTTGTCAAGTAGGAAAGAAGGGTGTAATTACTATTGAAAAAGGGAAGTCTACGGAAAACACTCTTCAAATTGTAGAAGGAATGCAGTTTGATCGTGGATACTTATCTCCTTACTTTGTCACTGACCGACAAAAAATGATAGTGGAGTTTCAAGAGTGTAAGTTGCTTTTGGTTGACGAAAAAATTACACACCCGGAGGAGCTGTTTAAGATATTGGACAATGCAGTGAAAGAGAAGTATCCAATTGTGATAGTTGCCGAGGGGATTGAGCAGGAAGCTTTAACTCCAGTAATAAGAAACAAGCTCAGAGGTGCACTGAAAGCAGTCGCCATTAAGGCGCCTGCCTTCGGTGAACGCAAAAGCCACTACTTAGACGACATTGCCATTTTGACCGGAGCAACAGTTGTAAGAGAAGAGATGGGAATGATTTTACATAAGACTGGCCCGGAGGTATTGGGGACTGCTACTAAGGTTGTGATAACAAAGGACTCTACACTGATAGTTACTGATGGAAGCACTCGAGAAGCTGTTGACAAGAGGGTTTCTCAGATACAAAGGCTTGTTGAGAACACTAAAGAAGAATTTCAAAAGAAGATATTAAATGAAAGAATAGGAAGATTATCAGGGGGGATTGCTATTCTGCAGGTAGGAGCACAAACGCAAGTTGAGTTGAAGGATAAACAACTCAGAATTGAAGATGCTTTGAATGCAACCAAGGCAGCAATTGAGGAAGGTGTTGTAGTTGGTGGTGGCTGCTGCCTTTTGAGGCTCTCGAAAAAGGTGGATGCCATCAAAGAAGTTTTGGAAAATGAAGAACAGAAGATTGGAGCGGAAATATTCAAGAAAGCTCTAAGCTATCCCGCAAAACTGATAGCTAAAAATGCAGGTGTAAATGGCAGTGTTGTAGTAGAGAAGGTTCTATCAAATGCTGATATGGGTACGGATATAATGCTGCAAGAGACTGTTATGAGGACCTAA

Protein Analysis

523

Amino Acids

56.42

Weight (kDa)

6.4

Isoelectric Point (pI)

31.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 57 - 511 1.2e-68 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1469
Acc36I ACCTGC 2 cut(s) 1228, 1469
AccB1I GGYRCC 1 cut(s) 931
AccBSI CCGCTC 1 cut(s) 1424
AccI GTMKAC 1 cut(s) 644
AciI CCGC 2 cut(s) 1424, 1455
AclWI GGATC 1 cut(s) 528
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 1 cut(s) 1175
AcuI CTGAAG 1 cut(s) 426
AcyI GRCGYC 1 cut(s) 932
AfaI GTAC 1 cut(s) 1534
AfiI CCNNNNNNNGG 1 cut(s) 941
AgsI TTSAA 9 cut(s) 446, 632, 662, 746, 1181, 1267, 1289, 1300, 1435
AjnI CCWGG 1 cut(s) 351
AjuI GAANNNNNNNTTGG 2 cut(s) 1401, 1433
Alw21I GWGCWC 2 cut(s) 910, 1249
Alw26I GTCTC 1 cut(s) 1547
Alw44I GTGCAC 1 cut(s) 906
AlwI GGATC 1 cut(s) 528
Ama87I CYCGRG 1 cut(s) 1113
AoxI GGCC 3 cut(s) 354, 477, 1036
ApaLI GTGCAC 1 cut(s) 906
ApeKI GCWGC 7 cut(s) 220, 425, 542, 1313, 1347, 1350, 1546
ApoI RAATTY 1 cut(s) 1175
Asp700I GAANNNNTTC 2 cut(s) 267, 1176
AspLEI GCGC 1 cut(s) 934
AspS9I GGNCC 5 cut(s) 197, 236, 478, 1037, 1566
AsuC2I CCSGG 2 cut(s) 788, 1040
AsuHPI GGTGA 4 cut(s) 63, 172, 272, 956
AvaI CYCGRG 1 cut(s) 1113
AvaII GGWCC 3 cut(s) 197, 236, 1566
BaeGI GKGCMC 1 cut(s) 910
BanI GGYRCC 1 cut(s) 931
BauI CACGAG 1 cut(s) 453
BbsI GAAGAC 1 cut(s) 473
Bbv12I GWGCWC 2 cut(s) 910, 1249
BbvI GCAGC 7 cut(s) 207, 437, 529, 1325, 1334, 1337, 1533
BccI CCATC 7 cut(s) 29, 122, 251, 356, 1006, 1097, 1391
BcgI CGANNNNNNTGC 2 cut(s) 668, 702
BciT130I CCWGG 1 cut(s) 353
BciVI GTATCC 2 cut(s) 686, 843
BcnI CCSGG 2 cut(s) 788, 1040
BcoDI GTCTC 1 cut(s) 1547
BfaI CTAG 1 cut(s) 482
BfmI CTRYAG 6 cut(s) 221, 372, 423, 471, 543, 1235
BfoI RGCGCY 1 cut(s) 935
BfuAI ACCTGC 2 cut(s) 1228, 1469
BfuI GTATCC 2 cut(s) 686, 843
BglI GCCNNNNNGGC 1 cut(s) 929
BisI GCNGC 7 cut(s) 221, 426, 543, 1314, 1348, 1351, 1547
BlsI GCNGC 7 cut(s) 222, 427, 544, 1315, 1349, 1352, 1548
Bme1390I CCNGG 3 cut(s) 353, 788, 1040
Bme18I GGWCC 3 cut(s) 197, 236, 1566
BmeT110I CYCGRG 1 cut(s) 1113
BmgT120I GGNCC 5 cut(s) 197, 236, 478, 1037, 1566
BmiI GGNNCC 3 cut(s) 198, 367, 933
BmrFI CCNGG 3 cut(s) 353, 788, 1040
BmsI GCATC 2 cut(s) 1282, 1369
BpiI GAAGAC 1 cut(s) 473
BplI GAGNNNNNCTC 2 cut(s) 1123, 1155
BpmI CTGGAG 2 cut(s) 354, 864
BpuMI CCSGG 2 cut(s) 788, 1040
BsaBI GATNNNNATC 1 cut(s) 697
BsaHI GRCGYC 1 cut(s) 932
BsaJI CCNNGG 2 cut(s) 852, 1308
BsaWI WCCGGW 1 cut(s) 986
BsaXI ACNNNNNCTCC 4 cut(s) 782, 812, 981, 1011
Bsc4I CCNNNNNNNGG 1 cut(s) 941
Bse1I ACTGG 2 cut(s) 881, 1039
Bse3DI GCAATG 2 cut(s) 565, 972
Bse8I GATNNNNATC 1 cut(s) 697
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 2 cut(s) 852, 1308
BseGI GGATG 1 cut(s) 1384
BseJI GATNNNNATC 1 cut(s) 697
BseLI CCNNNNNNNGG 1 cut(s) 941
BseMI GCAATG 2 cut(s) 565, 972
BseMII CTCAG 3 cut(s) 913, 1154, 1294
BseNI ACTGG 2 cut(s) 881, 1039
BseRI GAGGAG 1 cut(s) 806
BseSI GKGCMC 1 cut(s) 910
BseXI GCAGC 7 cut(s) 207, 437, 529, 1325, 1334, 1337, 1533
BsgI GTGCAG 2 cut(s) 12, 573
BshFI GGCC 3 cut(s) 356, 479, 1038
BshNI GGYRCC 1 cut(s) 931
BsiHKAI GWGCWC 2 cut(s) 910, 1249
BsiHKCI CYCGRG 1 cut(s) 1113
BsiSI CCGG 4 cut(s) 357, 788, 987, 1040
BslFI GGGAC 1 cut(s) 1066
BslI CCNNNNNNNGG 1 cut(s) 941
BsmAI GTCTC 1 cut(s) 1547
BsmFI GGGAC 1 cut(s) 1066
BsmI GAATGC 2 cut(s) 681, 1306
BsnI GGCC 3 cut(s) 356, 479, 1038
BsoBI CYCGRG 1 cut(s) 1113
Bsp1286I GDGCHC 2 cut(s) 910, 1249
Bsp143I GATC 3 cut(s) 279, 520, 685
BspACI CCGC 2 cut(s) 1424, 1455
BspANI GGCC 3 cut(s) 356, 479, 1038
BspCNI CTCAG 3 cut(s) 912, 1153, 1293
BspHI TCATGA 1 cut(s) 523
BspLI GGNNCC 3 cut(s) 198, 367, 933
BspMAI CTGCAG 5 cut(s) 225, 427, 475, 547, 1239
BspMI ACCTGC 2 cut(s) 1228, 1469
BspPI GGATC 1 cut(s) 528
BspT107I GGYRCC 1 cut(s) 931
BsrBI CCGCTC 1 cut(s) 1424
BsrDI GCAATG 2 cut(s) 565, 972
BsrI ACTGG 2 cut(s) 881, 1039
BssECI CCNNGG 2 cut(s) 852, 1308
BssMI GATC 3 cut(s) 279, 520, 685
BssNI GRCGYC 1 cut(s) 932
BssSI CACGAG 1 cut(s) 453
BssT1I CCWWGG 1 cut(s) 1308
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 6 cut(s) 268, 321, 395, 571, 997, 1558
Bst6I CTCTTC 2 cut(s) 663, 1002
BstACI GRCGYC 1 cut(s) 932
BstAPI GCANNNNNTGC 2 cut(s) 539, 551
BstC8I GCNNGC 4 cut(s) 330, 430, 556, 936
BstDEI CTNAG 6 cut(s) 899, 964, 1062, 1140, 1280, 1445
BstF5I GGATG 1 cut(s) 1384
BstH2I RGCGCY 1 cut(s) 935
BstHHI GCGC 1 cut(s) 934
BstKTI GATC 3 cut(s) 282, 523, 688
BstMAI GTCTC 1 cut(s) 1547
BstMBI GATC 3 cut(s) 279, 520, 685
BstMWI GCNNNNNNNGC 7 cut(s) 539, 551, 914, 929, 1301, 1310, 1475
BstNI CCWGG 1 cut(s) 353
BstSCI CCNGG 3 cut(s) 351, 786, 1038
BstSFI CTRYAG 6 cut(s) 221, 372, 423, 471, 543, 1235
BstSLI GKGCMC 1 cut(s) 910
BstV1I GCAGC 7 cut(s) 207, 437, 529, 1325, 1334, 1337, 1533
BstV2I GAAGAC 1 cut(s) 473
BsuI GTATCC 2 cut(s) 686, 843
BsuRI GGCC 3 cut(s) 356, 479, 1038
BtsCI GGATG 1 cut(s) 1384
BtsI GCAGTG 5 cut(s) 50, 81, 480, 825, 1498
BtsIMutI CAGTG 9 cut(s) 50, 81, 480, 576, 714, 825, 908, 1088, 1498
BveI ACCTGC 2 cut(s) 1228, 1469
Cac8I GCNNGC 4 cut(s) 330, 430, 556, 936
CciI TCATGA 1 cut(s) 523
CfoI GCGC 1 cut(s) 934
Cfr13I GGNCC 5 cut(s) 197, 236, 478, 1037, 1566
Csp6I GTAC 1 cut(s) 1533
CviAII CATG 5 cut(s) 125, 166, 433, 524, 580
CviQI GTAC 1 cut(s) 1533
DdeI CTNAG 6 cut(s) 899, 964, 1062, 1140, 1280, 1445
DinI GGCGCC 1 cut(s) 933
DpnI GATC 3 cut(s) 281, 522, 687
DpnII GATC 3 cut(s) 279, 520, 685
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 2 cut(s) 663, 1002
EarI CTCTTC 2 cut(s) 663, 1002
Eco130I CCWWGG 1 cut(s) 1308
Eco47I GGWCC 3 cut(s) 197, 236, 1566
Eco57I CTGAAG 1 cut(s) 426
Eco88I CYCGRG 1 cut(s) 1113
EcoO109I RGGNCCY 1 cut(s) 1566
EcoRII CCWGG 1 cut(s) 351
EcoT14I CCWWGG 1 cut(s) 1308
EgeI GGCGCC 1 cut(s) 933
EheI GGCGCC 1 cut(s) 933
ErhI CCWWGG 1 cut(s) 1308
FaeI CATG 5 cut(s) 128, 169, 436, 527, 583
FalI AAGNNNNNCTT 2 cut(s) 52, 84
FaqI GGGAC 1 cut(s) 1066
FatI CATG 5 cut(s) 124, 165, 432, 523, 579
FauI CCCGC 1 cut(s) 1462
FblI GTMKAC 1 cut(s) 644
Fnu4HI GCNGC 7 cut(s) 221, 426, 543, 1314, 1348, 1351, 1547
FokI GGATG 1 cut(s) 1391
Fsp4HI GCNGC 7 cut(s) 221, 426, 543, 1314, 1348, 1351, 1547
FspBI CTAG 1 cut(s) 482
GlaI GCGC 1 cut(s) 933
GluI GCNGC 7 cut(s) 221, 426, 543, 1314, 1348, 1351, 1547
GsuI CTGGAG 2 cut(s) 354, 864
HaeII RGCGCY 1 cut(s) 935
HaeIII GGCC 3 cut(s) 356, 479, 1038
HapII CCGG 4 cut(s) 357, 788, 987, 1040
HhaI GCGC 1 cut(s) 934
Hin1I GRCGYC 1 cut(s) 932
Hin1II CATG 5 cut(s) 128, 169, 436, 527, 583
Hin6I GCGC 1 cut(s) 932
HinP1I GCGC 1 cut(s) 932
HincII GTYRAC 2 cut(s) 769, 1126
HindII GTYRAC 2 cut(s) 769, 1126
HindIII AAGCTT 2 cut(s) 146, 870
HinfI GANTC 3 cut(s) 293, 436, 1082
HpaII CCGG 4 cut(s) 357, 788, 987, 1040
HphI GGTGA 4 cut(s) 63, 172, 272, 956
Hpy166II GTNNAC 5 cut(s) 645, 769, 908, 947, 1126
Hpy188I TCNGA 5 cut(s) 311, 490, 902, 1143, 1283
Hpy188III TCNNGA 6 cut(s) 507, 524, 746, 1115, 1366, 1435
Hpy8I GTNNAC 5 cut(s) 645, 769, 908, 947, 1126
HpyAV CCTTC 9 cut(s) 201, 401, 409, 607, 665, 949, 1261, 1319, 1501
HpyCH4III ACNGT 6 cut(s) 268, 321, 395, 571, 997, 1558
HpyF10VI GCNNNNNNNGC 7 cut(s) 539, 551, 914, 929, 1301, 1310, 1475
HpyF3I CTNAG 6 cut(s) 899, 964, 1062, 1140, 1280, 1445
Hsp92I GRCGYC 1 cut(s) 932
Hsp92II CATG 5 cut(s) 128, 169, 436, 527, 583
HspAI GCGC 1 cut(s) 932
KasI GGCGCC 1 cut(s) 931
Kzo9I GATC 3 cut(s) 279, 520, 685
LmnI GCTCC 5 cut(s) 335, 793, 989, 1244, 1421
Lsp1109I GCAGC 7 cut(s) 207, 437, 529, 1325, 1334, 1337, 1533
LweI GCATC 2 cut(s) 1282, 1369
MaeI CTAG 1 cut(s) 482
MaeIII GTNAC 4 cut(s) 187, 418, 712, 1096
MalI GATC 3 cut(s) 281, 522, 687
MbiI CCGCTC 1 cut(s) 1424
MboI GATC 3 cut(s) 279, 520, 685
MfeI CAATTG 2 cut(s) 837, 1317
MhlI GDGCHC 2 cut(s) 910, 1249
Mly113I GGCGCC 1 cut(s) 932
MlyI GAGTC 2 cut(s) 287, 1076
MmeI TCCRAC 3 cut(s) 38, 267, 289
MroXI GAANNNNTTC 2 cut(s) 267, 1176
MseI TTAA 8 cut(s) 120, 315, 323, 398, 801, 875, 927, 1193
MspA1I CMGCKG 2 cut(s) 377, 428
MspI CCGG 4 cut(s) 357, 788, 987, 1040
MspR9I CCNGG 3 cut(s) 353, 788, 1040
MunI CAATTG 2 cut(s) 837, 1317
Mva1269I GAATGC 2 cut(s) 681, 1306
MvaI CCWGG 1 cut(s) 353
MwoI GCNNNNNNNGC 7 cut(s) 539, 551, 914, 929, 1301, 1310, 1475
NarI GGCGCC 1 cut(s) 932
NciI CCSGG 2 cut(s) 788, 1040
NdeII GATC 3 cut(s) 279, 520, 685
NlaIII CATG 5 cut(s) 128, 169, 436, 527, 583
NlaIV GGNNCC 3 cut(s) 198, 367, 933
NmeAIII GCCGAG 1 cut(s) 877
NmuCI GTSAC 1 cut(s) 712
PaeR7I CTCGAG 1 cut(s) 1113
PagI TCATGA 1 cut(s) 523
PaqCI CACCTGC 1 cut(s) 1469
PctI GAATGC 2 cut(s) 681, 1306
PdmI GAANNNNTTC 2 cut(s) 267, 1176
PfeI GAWTC 1 cut(s) 436
PkrI GCNGC 7 cut(s) 222, 427, 544, 1315, 1349, 1352, 1548
PleI GAGTC 2 cut(s) 287, 1076
PluTI GGCGCC 1 cut(s) 935
PpsI GAGTC 2 cut(s) 287, 1076
PpuMI RGGWCCY 1 cut(s) 1566
Psp5II RGGWCCY 1 cut(s) 1566
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 3 cut(s) 198, 367, 933
PspPI GGNCC 5 cut(s) 197, 236, 478, 1037, 1566
PspPPI RGGWCCY 1 cut(s) 1566
PsrI GAACNNNNNNTAC 4 cut(s) 569, 601, 1494, 1526
PstI CTGCAG 5 cut(s) 225, 427, 475, 547, 1239
PvuII CAGCTG 2 cut(s) 377, 428
RsaI GTAC 1 cut(s) 1534
RsaNI GTAC 1 cut(s) 1533
SaqAI TTAA 8 cut(s) 120, 315, 323, 398, 801, 875, 927, 1193
SatI GCNGC 7 cut(s) 221, 426, 543, 1314, 1348, 1351, 1547
Sau3AI GATC 3 cut(s) 279, 520, 685
Sau96I GGNCC 5 cut(s) 197, 236, 478, 1037, 1566
SchI GAGTC 2 cut(s) 287, 1076
ScrFI CCNGG 3 cut(s) 353, 788, 1040
SduI GDGCHC 2 cut(s) 910, 1249
SfaNI GCATC 2 cut(s) 1282, 1369
SfcI CTRYAG 6 cut(s) 221, 372, 423, 471, 543, 1235
SfoI GGCGCC 1 cut(s) 933
Sfr274I CTCGAG 1 cut(s) 1113
SinI GGWCC 3 cut(s) 197, 236, 1566
SlaI CTCGAG 1 cut(s) 1113
SmlI CTYRAG 1 cut(s) 1113
SmoI CTYRAG 1 cut(s) 1113
SsiI CCGC 2 cut(s) 1424, 1455
SspDI GGCGCC 1 cut(s) 931
SspI AATATT 1 cut(s) 1431
SspMI CTAG 1 cut(s) 482
StyD4I CCNGG 3 cut(s) 351, 786, 1038
StyI CCWWGG 1 cut(s) 1308
TaaI ACNGT 6 cut(s) 268, 321, 395, 571, 997, 1558
TaqI TCGA 3 cut(s) 282, 1114, 1367
TaqII GACCGA 1 cut(s) 735
TfiI GAWTC 1 cut(s) 436
Tru1I TTAA 8 cut(s) 120, 315, 323, 398, 801, 875, 927, 1193
Tru9I TTAA 8 cut(s) 120, 315, 323, 398, 801, 875, 927, 1193
TscAI CASTG 9 cut(s) 57, 88, 480, 576, 721, 825, 915, 1095, 1498
TseFI GTSAC 1 cut(s) 712
TseI GCWGC 7 cut(s) 220, 425, 542, 1313, 1347, 1350, 1546
Tsp45I GTSAC 1 cut(s) 712
TspDTI ATGAA 4 cut(s) 449, 540, 1212, 1419
TspGWI ACGGA 2 cut(s) 662, 1550
TspRI CASTG 9 cut(s) 57, 88, 480, 576, 721, 825, 915, 1095, 1498
VneI GTGCAC 1 cut(s) 906
VpaK11BI GGWCC 3 cut(s) 197, 236, 1566
XapI RAATTY 1 cut(s) 1175
XhoI CTCGAG 1 cut(s) 1113
XmiI GTMKAC 1 cut(s) 644
XmnI GAANNNNTTC 2 cut(s) 267, 1176
XspI CTAG 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.