RchiOBHm_Chr5g0024441

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0018612
Physical Location & Seq
Forward (+)
18573874 .. 18574668
795 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30419

Sequence Viewer

Length: 795 bp
ATGGAATACAGTCTGTGGCTCTACGATTACAATACCCTAAACTCTCCGAATTCTTCCTATTTGAGATCCACGATGGGTTTTGCTACAGAACGCGAAAAATTGGTCTACACTGCTAAGCTGGCTCAGGAGGCAGAGCGATACGATGAGATGGTGGATGCAATCAAGATAGTTGCAAATCTTGATGTTGAATTGACCCTTGAAGAGAGAAGCTACCTCTCTTTTGGGTACAAGAATGTTGTTGATGCTCGGAGATCATCATGGAGGATCTTATCATCAACAGAGCAGACGGAGGGGGCGAAAGGAAATAATATTCACATGAAGCATCTAAAGAAATATAGACAGACAGTTGAATCAGAGATATCAATTATTTGTAGAGATATCATCTCAGTGCTTGATAGTCATCTCATCCCTTCGACTTCAGATGGTGAATCAACTGTGCATTATCATAAGATGAAAGGAGACTTTTATAGGTATCTCGCAGAAATCAAGGCCGGTGATGAGAAAGAGGAGGCTATCAAACAGTCGAAGAAAGCATATTTCAAGGCCCTCACTGTTGCAGAAGCTGAGTTGCCTCCTACAAATCCCATCCGTTTGGGTGCGGCTTTGCACTTGTCAATCTTATATCATGAGTTCCTGAATAATCCGGAGACGGCCTGCCGTCTTACAAAGATAGCTTTTGAGGAAGGCACTGCTGAGATTAATACACTAAACAATGAACCATACAAGAATACCCTCATGGCCTTGAAACTTTTAAGGGAAAACTACAGCTTGTGGACTTCCCAACTTCCACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

264

Amino Acids

30.33

Weight (kDa)

5.95

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 37 - 259 1.5e-80 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018612)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0024421 RchiOBHm_Chr5g0024441
rosa_laevigata RLG00000032798
rosa_multiflora Rmu_sc0005861.1_g000014
rosa_rugosa Rorug05G0083400
rosa_samantha Rh5AG174400 Rh5BG172200 Rh5CG188100 Rh5DG173600
rosa_wichuraiana Rw5G015750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 105
AccII CGCG 1 cut(s) 93
AccIII TCCGGA 1 cut(s) 643
AciI CCGC 1 cut(s) 599
AclWI GGATC 2 cut(s) 60, 272
AcsI RAATTY 1 cut(s) 49
AcuI CTGAAG 1 cut(s) 402
AfaI GTAC 1 cut(s) 227
AgsI TTSAA 5 cut(s) 188, 200, 350, 541, 745
AluBI AGCT 5 cut(s) 118, 210, 563, 674, 768
AluI AGCT 5 cut(s) 118, 210, 563, 674, 768
Alw26I GTCTC 2 cut(s) 453, 641
AlwI GGATC 2 cut(s) 60, 272
AlwNI CAGNNNCTG 1 cut(s) 563
Aor13HI TCCGGA 1 cut(s) 643
AoxI GGCC 4 cut(s) 489, 543, 651, 738
ApoI RAATTY 1 cut(s) 49
ArsI GACNNNNNNTTYG 2 cut(s) 87, 119
AseI ATTAAT 1 cut(s) 699
AspS9I GGNCC 1 cut(s) 544
AsuHPI GGTGA 2 cut(s) 437, 506
BccI CCATC 4 cut(s) 67, 142, 416, 593
BceAI ACGGC 2 cut(s) 642, 666
BcoDI GTCTC 2 cut(s) 453, 641
BfmI CTRYAG 2 cut(s) 84, 763
BisI GCNGC 1 cut(s) 600
BlpI GCTNAGC 1 cut(s) 114
BlsI GCNGC 1 cut(s) 601
BmgT120I GGNCC 1 cut(s) 544
BmsI GCATC 3 cut(s) 145, 232, 331
Bpu10I CCTNAGC 1 cut(s) 123
Bpu1102I GCTNAGC 1 cut(s) 114
BsaBI GATNNNNATC 1 cut(s) 399
BsaWI WCCGGW 1 cut(s) 643
Bse118I RCCGGY 1 cut(s) 491
Bse8I GATNNNNATC 1 cut(s) 399
BseAI TCCGGA 1 cut(s) 643
BseGI GGATG 3 cut(s) 160, 405, 585
BseJI GATNNNNATC 1 cut(s) 399
BseMII CTCAG 4 cut(s) 137, 399, 555, 684
BseRI GAGGAG 1 cut(s) 521
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 4 cut(s) 491, 545, 653, 740
BsiSI CCGG 2 cut(s) 492, 644
BsmAI GTCTC 2 cut(s) 453, 641
BsmBI CGTCTC 1 cut(s) 641
BsnI GGCC 4 cut(s) 491, 545, 653, 740
Bsp13I TCCGGA 1 cut(s) 643
Bsp143I GATC 3 cut(s) 65, 251, 264
Bsp1720I GCTNAGC 1 cut(s) 114
BspACI CCGC 1 cut(s) 599
BspANI GGCC 4 cut(s) 491, 545, 653, 740
BspCNI CTCAG 4 cut(s) 136, 398, 556, 685
BspEI TCCGGA 1 cut(s) 643
BspFNI CGCG 1 cut(s) 93
BspHI TCATGA 1 cut(s) 625
BspPI GGATC 2 cut(s) 60, 272
BsrFI RCCGGY 1 cut(s) 491
BssAI RCCGGY 1 cut(s) 491
BssMI GATC 3 cut(s) 65, 251, 264
Bst4CI ACNGT 5 cut(s) 11, 346, 436, 522, 553
Bst6I CTCTTC 1 cut(s) 195
BstC8I GCNNGC 2 cut(s) 120, 655
BstDEI CTNAG 5 cut(s) 114, 123, 385, 564, 693
BstF5I GGATG 3 cut(s) 160, 405, 585
BstFNI CGCG 1 cut(s) 93
BstKTI GATC 3 cut(s) 68, 254, 267
BstMAI GTCTC 2 cut(s) 453, 641
BstMBI GATC 3 cut(s) 65, 251, 264
BstMWI GCNNNNNNNGC 2 cut(s) 119, 128
BstSFI CTRYAG 2 cut(s) 84, 763
BstUI CGCG 1 cut(s) 93
BstX2I RGATCY 2 cut(s) 65, 264
BstXI CCANNNNNNTGG 1 cut(s) 592
BstYI RGATCY 2 cut(s) 65, 264
BsuRI GGCC 4 cut(s) 491, 545, 653, 740
BtsCI GGATG 3 cut(s) 160, 405, 585
BtsI GCAGTG 2 cut(s) 108, 687
BtsIMutI CAGTG 4 cut(s) 108, 393, 549, 687
Cac8I GCNNGC 2 cut(s) 120, 655
CaiI CAGNNNCTG 1 cut(s) 563
CciI TCATGA 1 cut(s) 625
Cfr10I RCCGGY 1 cut(s) 491
Cfr13I GGNCC 1 cut(s) 544
Csp6I GTAC 1 cut(s) 226
CviAII CATG 4 cut(s) 258, 316, 626, 736
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 5 cut(s) 114, 123, 385, 564, 693
DpnI GATC 3 cut(s) 67, 253, 266
DpnII GATC 3 cut(s) 65, 251, 264
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Eco32I GATATC 2 cut(s) 360, 379
Eco57I CTGAAG 1 cut(s) 402
EcoO109I RGGNCCY 1 cut(s) 544
EcoRI GAATTC 1 cut(s) 49
EcoRV GATATC 2 cut(s) 360, 379
Esp3I CGTCTC 1 cut(s) 641
FaeI CATG 4 cut(s) 261, 319, 629, 739
FatI CATG 4 cut(s) 257, 315, 625, 735
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 1 cut(s) 600
FokI GGATG 3 cut(s) 167, 392, 572
Fsp4HI GCNGC 1 cut(s) 600
GluI GCNGC 1 cut(s) 600
HaeIII GGCC 4 cut(s) 491, 545, 653, 740
HapII CCGG 2 cut(s) 492, 644
Hin1II CATG 4 cut(s) 261, 319, 629, 739
HinfI GANTC 2 cut(s) 350, 428
HpaII CCGG 2 cut(s) 492, 644
HphI GGTGA 2 cut(s) 437, 506
Hpy166II GTNNAC 2 cut(s) 106, 774
Hpy188I TCNGA 4 cut(s) 48, 249, 355, 421
Hpy188III TCNNGA 6 cut(s) 125, 163, 179, 626, 634, 644
Hpy8I GTNNAC 2 cut(s) 106, 774
HpyAV CCTTC 2 cut(s) 420, 677
HpyCH4III ACNGT 5 cut(s) 11, 346, 436, 522, 553
HpyCH4V TGCA 5 cut(s) 158, 173, 439, 557, 607
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 128
HpyF3I CTNAG 5 cut(s) 114, 123, 385, 564, 693
Hsp92II CATG 4 cut(s) 261, 319, 629, 739
Kpn2I TCCGGA 1 cut(s) 643
Kzo9I GATC 3 cut(s) 65, 251, 264
LpnPI CCDG 6 cut(s) 104, 110, 505, 647, 657, 667
LweI GCATC 3 cut(s) 145, 232, 331
MalI GATC 3 cut(s) 67, 253, 266
MboI GATC 3 cut(s) 65, 251, 264
MboII GAAGA 3 cut(s) 45, 212, 538
MflI RGATCY 2 cut(s) 65, 264
MluCI AATT 4 cut(s) 49, 98, 188, 363
MroI TCCGGA 1 cut(s) 643
MseI TTAA 3 cut(s) 699, 752, 793
MslI CAYNNNNRTG 1 cut(s) 386
MspI CCGG 2 cut(s) 492, 644
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 2 cut(s) 119, 128
NdeII GATC 3 cut(s) 65, 251, 264
NlaIII CATG 4 cut(s) 261, 319, 629, 739
PagI TCATGA 1 cut(s) 625
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PfeI GAWTC 2 cut(s) 350, 428
PkrI GCNGC 1 cut(s) 601
PshBI ATTAAT 1 cut(s) 699
PspPI GGNCC 1 cut(s) 544
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 2 cut(s) 65, 264
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
RseI CAYNNNNRTG 1 cut(s) 386
SaqAI TTAA 3 cut(s) 699, 752, 793
SatI GCNGC 1 cut(s) 600
Sau3AI GATC 3 cut(s) 65, 251, 264
Sau96I GGNCC 1 cut(s) 544
SetI ASST 7 cut(s) 120, 212, 216, 473, 565, 676, 770
SfaNI GCATC 3 cut(s) 145, 232, 331
SfcI CTRYAG 2 cut(s) 84, 763
SmiMI CAYNNNNRTG 1 cut(s) 386
Sse9I AATT 4 cut(s) 49, 98, 188, 363
SsiI CCGC 1 cut(s) 599
SspI AATATT 1 cut(s) 310
TaaI ACNGT 5 cut(s) 11, 346, 436, 522, 553
TaqI TCGA 2 cut(s) 413, 524
TasI AATT 4 cut(s) 49, 98, 188, 363
TauI GCSGC 1 cut(s) 602
TfiI GAWTC 2 cut(s) 350, 428
Tru1I TTAA 3 cut(s) 699, 752, 793
Tru9I TTAA 3 cut(s) 699, 752, 793
TscAI CASTG 4 cut(s) 115, 393, 556, 694
TspDTI ATGAA 3 cut(s) 332, 467, 729
TspGWI ACGGA 2 cut(s) 302, 578
TspRI CASTG 4 cut(s) 115, 393, 556, 694
VspI ATTAAT 1 cut(s) 699
XapI RAATTY 1 cut(s) 49
XmiI GTMKAC 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.