Rh5DG173600

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018612
Physical Location & Seq
Forward (+)
18622071 .. 18623018
948 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG173600.1

Sequence Viewer

Length: 723 bp
ATGGGTTTTGCTACAGAACGCGAAAAATTGGTCTACACTGCTAAGCTGGCTCAGGAGGCAGAGCGATACGATGAGATGGTGGATGCAATCAAGATAGTTGCAAATCTTGATGTTGAATTGACCCTTGAAGAGAGAAGCTACCTCTCTTTTGGGTACAAGAATGTTGTTGATGCTCGGAGATCATCATGGAGGATCTTATCATCAACAGAGCAGACGGAGGGGGCGAAAGGAAATAATATTCACATGAAGCATCTAAAGAAATATAGACAGACAGTTGAATCAGAGATATCAATTATTTGTAGAGATATCATCTCAGTGCTTGATAGTCATCTCATCCCTTCGACTTCAGATGGTGAATCAACTGTGCATTATCATAAGATGAAAGGAGACTTTTATAGGTATCTCGCAGAAATCAAGGCCGGTGATGAGAAAGAGGAGGCTATCAAACAGTCGAAGAAAGCATATTTCAAGGCCCTCACTGTTGCAGAAGCTGAGTTGCCTCCTACAAATCCCATCCGTTTGGGTGCGGCTTTGCACTTGTCAATCTTATATCATGAGTTCCTGAATAATCCGGAGACGGCCTGCCGTCTTACAAAGATAGCTTTTGAGGAAGGCACTGCTGAGATTAATACACTAAACAATGAACCATACAAGAATACCCTCATGGCCTTGAAACTTTTAAGGGAAAACTACAGCTTGTGGACTTCCCAACTTCCACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

240

Amino Acids

27.43

Weight (kDa)

6.1

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 13 - 235 1.1e-80 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018612)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0024421 RchiOBHm_Chr5g0024441
rosa_laevigata RLG00000032798
rosa_multiflora Rmu_sc0005861.1_g000014
rosa_rugosa Rorug05G0083400
rosa_samantha Rh5AG174400 Rh5BG172200 Rh5CG188100 Rh5DG173600
rosa_wichuraiana Rw5G015750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 33
AccII CGCG 1 cut(s) 21
AccIII TCCGGA 1 cut(s) 571
AciI CCGC 1 cut(s) 527
AclWI GGATC 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 1 cut(s) 155
AgsI TTSAA 5 cut(s) 116, 128, 278, 469, 673
AluBI AGCT 5 cut(s) 46, 138, 491, 602, 696
AluI AGCT 5 cut(s) 46, 138, 491, 602, 696
Alw26I GTCTC 2 cut(s) 381, 569
AlwI GGATC 1 cut(s) 200
AlwNI CAGNNNCTG 1 cut(s) 491
Aor13HI TCCGGA 1 cut(s) 571
AoxI GGCC 4 cut(s) 417, 471, 579, 666
ArsI GACNNNNNNTTYG 2 cut(s) 15, 47
AseI ATTAAT 1 cut(s) 627
AspS9I GGNCC 1 cut(s) 472
AsuHPI GGTGA 2 cut(s) 365, 434
BccI CCATC 3 cut(s) 70, 344, 521
BceAI ACGGC 2 cut(s) 570, 594
BcoDI GTCTC 2 cut(s) 381, 569
BfmI CTRYAG 2 cut(s) 12, 691
BisI GCNGC 1 cut(s) 528
BlpI GCTNAGC 1 cut(s) 42
BlsI GCNGC 1 cut(s) 529
BmgT120I GGNCC 1 cut(s) 472
BmsI GCATC 3 cut(s) 73, 160, 259
Bpu10I CCTNAGC 1 cut(s) 51
Bpu1102I GCTNAGC 1 cut(s) 42
BsaBI GATNNNNATC 1 cut(s) 327
BsaWI WCCGGW 1 cut(s) 571
Bse118I RCCGGY 1 cut(s) 419
Bse8I GATNNNNATC 1 cut(s) 327
BseAI TCCGGA 1 cut(s) 571
BseGI GGATG 3 cut(s) 88, 333, 513
BseJI GATNNNNATC 1 cut(s) 327
BseMII CTCAG 4 cut(s) 65, 327, 483, 612
BseRI GAGGAG 1 cut(s) 449
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 4 cut(s) 419, 473, 581, 668
BsiSI CCGG 2 cut(s) 420, 572
BsmAI GTCTC 2 cut(s) 381, 569
BsmBI CGTCTC 1 cut(s) 569
BsnI GGCC 4 cut(s) 419, 473, 581, 668
Bsp13I TCCGGA 1 cut(s) 571
Bsp143I GATC 2 cut(s) 179, 192
Bsp1720I GCTNAGC 1 cut(s) 42
BspACI CCGC 1 cut(s) 527
BspANI GGCC 4 cut(s) 419, 473, 581, 668
BspCNI CTCAG 4 cut(s) 64, 326, 484, 613
BspEI TCCGGA 1 cut(s) 571
BspFNI CGCG 1 cut(s) 21
BspHI TCATGA 1 cut(s) 553
BspPI GGATC 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 419
BssAI RCCGGY 1 cut(s) 419
BssMI GATC 2 cut(s) 179, 192
Bst4CI ACNGT 4 cut(s) 274, 364, 450, 481
Bst6I CTCTTC 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 48, 583
BstDEI CTNAG 5 cut(s) 42, 51, 313, 492, 621
BstF5I GGATG 3 cut(s) 88, 333, 513
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 2 cut(s) 182, 195
BstMAI GTCTC 2 cut(s) 381, 569
BstMBI GATC 2 cut(s) 179, 192
BstMWI GCNNNNNNNGC 2 cut(s) 47, 56
BstSFI CTRYAG 2 cut(s) 12, 691
BstUI CGCG 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 192
BstXI CCANNNNNNTGG 1 cut(s) 520
BstYI RGATCY 1 cut(s) 192
BsuRI GGCC 4 cut(s) 419, 473, 581, 668
BtsCI GGATG 3 cut(s) 88, 333, 513
BtsI GCAGTG 2 cut(s) 36, 615
BtsIMutI CAGTG 4 cut(s) 36, 321, 477, 615
Cac8I GCNNGC 2 cut(s) 48, 583
CaiI CAGNNNCTG 1 cut(s) 491
CciI TCATGA 1 cut(s) 553
Cfr10I RCCGGY 1 cut(s) 419
Cfr13I GGNCC 1 cut(s) 472
Csp6I GTAC 1 cut(s) 154
CviAII CATG 4 cut(s) 186, 244, 554, 664
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 5 cut(s) 42, 51, 313, 492, 621
DpnI GATC 2 cut(s) 181, 194
DpnII GATC 2 cut(s) 179, 192
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco32I GATATC 2 cut(s) 288, 307
Eco57I CTGAAG 1 cut(s) 330
EcoO109I RGGNCCY 1 cut(s) 472
EcoRV GATATC 2 cut(s) 288, 307
Esp3I CGTCTC 1 cut(s) 569
FaeI CATG 4 cut(s) 189, 247, 557, 667
FatI CATG 4 cut(s) 185, 243, 553, 663
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 1 cut(s) 528
FokI GGATG 3 cut(s) 95, 320, 500
Fsp4HI GCNGC 1 cut(s) 528
GluI GCNGC 1 cut(s) 528
HaeIII GGCC 4 cut(s) 419, 473, 581, 668
HapII CCGG 2 cut(s) 420, 572
Hin1II CATG 4 cut(s) 189, 247, 557, 667
HinfI GANTC 2 cut(s) 278, 356
HpaII CCGG 2 cut(s) 420, 572
HphI GGTGA 2 cut(s) 365, 434
Hpy166II GTNNAC 2 cut(s) 34, 702
Hpy188I TCNGA 3 cut(s) 177, 283, 349
Hpy188III TCNNGA 6 cut(s) 53, 91, 107, 554, 562, 572
Hpy8I GTNNAC 2 cut(s) 34, 702
HpyAV CCTTC 2 cut(s) 348, 605
HpyCH4III ACNGT 4 cut(s) 274, 364, 450, 481
HpyCH4V TGCA 5 cut(s) 86, 101, 367, 485, 535
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 56
HpyF3I CTNAG 5 cut(s) 42, 51, 313, 492, 621
Hsp92II CATG 4 cut(s) 189, 247, 557, 667
Kpn2I TCCGGA 1 cut(s) 571
Kzo9I GATC 2 cut(s) 179, 192
LpnPI CCDG 6 cut(s) 32, 38, 433, 575, 585, 595
LweI GCATC 3 cut(s) 73, 160, 259
MalI GATC 2 cut(s) 181, 194
MboI GATC 2 cut(s) 179, 192
MboII GAAGA 2 cut(s) 140, 466
MflI RGATCY 1 cut(s) 192
MluCI AATT 3 cut(s) 26, 116, 291
MroI TCCGGA 1 cut(s) 571
MseI TTAA 3 cut(s) 627, 680, 721
MslI CAYNNNNRTG 1 cut(s) 314
MspI CCGG 2 cut(s) 420, 572
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 2 cut(s) 47, 56
NdeII GATC 2 cut(s) 179, 192
NlaIII CATG 4 cut(s) 189, 247, 557, 667
PagI TCATGA 1 cut(s) 553
PcsI WCGNNNNNNNCGW 1 cut(s) 221
PfeI GAWTC 2 cut(s) 278, 356
PkrI GCNGC 1 cut(s) 529
PshBI ATTAAT 1 cut(s) 627
PspPI GGNCC 1 cut(s) 472
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 1 cut(s) 192
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 314
SaqAI TTAA 3 cut(s) 627, 680, 721
SatI GCNGC 1 cut(s) 528
Sau3AI GATC 2 cut(s) 179, 192
Sau96I GGNCC 1 cut(s) 472
SetI ASST 7 cut(s) 48, 140, 144, 401, 493, 604, 698
SfaNI GCATC 3 cut(s) 73, 160, 259
SfcI CTRYAG 2 cut(s) 12, 691
SmiMI CAYNNNNRTG 1 cut(s) 314
Sse9I AATT 3 cut(s) 26, 116, 291
SsiI CCGC 1 cut(s) 527
SspI AATATT 1 cut(s) 238
TaaI ACNGT 4 cut(s) 274, 364, 450, 481
TaqI TCGA 2 cut(s) 341, 452
TasI AATT 3 cut(s) 26, 116, 291
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 2 cut(s) 278, 356
Tru1I TTAA 3 cut(s) 627, 680, 721
Tru9I TTAA 3 cut(s) 627, 680, 721
TscAI CASTG 4 cut(s) 43, 321, 484, 622
TspDTI ATGAA 3 cut(s) 260, 395, 657
TspGWI ACGGA 2 cut(s) 230, 506
TspRI CASTG 4 cut(s) 43, 321, 484, 622
VspI ATTAAT 1 cut(s) 627
XmiI GTMKAC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.