Rh5BG172200

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0018612
Physical Location & Seq
Forward (+)
18047845 .. 18048492
648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG172200.1

Sequence Viewer

Length: 648 bp
ATGGTGGATGCAATGAAGATGGTTGCAAATCTTGATGTTGAATTGACCCTTGAAGAGAGAAGCTACCTCTCTTTTGGGTACAAGAATGTTGTTGATGCTCGGAGATCATCATGGAGGATCTTATCATCAACAGAGCAGACGGAGGTGGCGAAAGGAAATAATATTCACATGAAGCATCTAAAGAAATATAGACAGAGAGTTGAATCAGAGATATCAATTATTTGTAGAGATATCATCTCAGTGCTTGATAGTCATCTCATCCCTTCGACTTCAGATGGTGAATCAACTGTGCATTATCATAAGATGAAAGGAGACTTTTATAGGTATCTCGCAGAAATCAAGGCCGGTGATGAGAAAGAGGAGGCTATTAAACAGTCGAAGAAAGCATATTACAAGGCCCTCACTGTTGCAGAAGCTGAGTTGCCTCCTACAAATCCCACCCGTTTGGGTGCGGCTTTGCACTTGTCAATCTTATATCATGAGTTCCTGAATAATCCGGAGACGGCCTGCCGTCTTACAAAGATAGCTTTTGAGGAAGGCACTGCTGAGATTAACACACTGAACAATGAACCATACAAGAATACCCTCATGGCCTTGAAACTTTTAAGGGAAAACTACAGCTTGTGGACTTCCCAACTGCCACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

215

Amino Acids

24.64

Weight (kDa)

6.72

Isoelectric Point (pI)

49.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 1 - 210 8.2e-75 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018612)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0024421 RchiOBHm_Chr5g0024441
rosa_laevigata RLG00000032798
rosa_multiflora Rmu_sc0005861.1_g000014
rosa_rugosa Rorug05G0083400
rosa_samantha Rh5AG174400 Rh5BG172200 Rh5CG188100 Rh5DG173600
rosa_wichuraiana Rw5G015750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 496
AciI CCGC 1 cut(s) 452
AclWI GGATC 1 cut(s) 125
AcuI CTGAAG 1 cut(s) 255
AfaI GTAC 1 cut(s) 80
AgsI TTSAA 4 cut(s) 41, 53, 203, 598
AluBI AGCT 4 cut(s) 63, 416, 527, 621
AluI AGCT 4 cut(s) 63, 416, 527, 621
Alw26I GTCTC 2 cut(s) 306, 494
AlwI GGATC 1 cut(s) 125
AlwNI CAGNNNCTG 1 cut(s) 416
Aor13HI TCCGGA 1 cut(s) 496
AoxI GGCC 4 cut(s) 342, 396, 504, 591
AspS9I GGNCC 1 cut(s) 397
AsuHPI GGTGA 2 cut(s) 290, 359
BccI CCATC 2 cut(s) 13, 269
BceAI ACGGC 2 cut(s) 495, 519
BcoDI GTCTC 2 cut(s) 306, 494
BfmI CTRYAG 1 cut(s) 616
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
BmgT120I GGNCC 1 cut(s) 397
BmsI GCATC 2 cut(s) 85, 184
BsaBI GATNNNNATC 1 cut(s) 252
BsaWI WCCGGW 1 cut(s) 496
Bse118I RCCGGY 1 cut(s) 344
Bse3DI GCAATG 1 cut(s) 18
Bse8I GATNNNNATC 1 cut(s) 252
BseAI TCCGGA 1 cut(s) 496
BseGI GGATG 2 cut(s) 13, 258
BseJI GATNNNNATC 1 cut(s) 252
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 3 cut(s) 252, 408, 537
BseRI GAGGAG 1 cut(s) 374
BshFI GGCC 4 cut(s) 344, 398, 506, 593
BsiSI CCGG 2 cut(s) 345, 497
BsmAI GTCTC 2 cut(s) 306, 494
BsmBI CGTCTC 1 cut(s) 494
BsnI GGCC 4 cut(s) 344, 398, 506, 593
Bsp13I TCCGGA 1 cut(s) 496
Bsp143I GATC 2 cut(s) 104, 117
BspACI CCGC 1 cut(s) 452
BspANI GGCC 4 cut(s) 344, 398, 506, 593
BspCNI CTCAG 3 cut(s) 251, 409, 538
BspEI TCCGGA 1 cut(s) 496
BspHI TCATGA 1 cut(s) 478
BspPI GGATC 1 cut(s) 125
BsrDI GCAATG 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 344
BssAI RCCGGY 1 cut(s) 344
BssMI GATC 2 cut(s) 104, 117
Bst4CI ACNGT 3 cut(s) 289, 375, 406
Bst6I CTCTTC 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 508
BstDEI CTNAG 3 cut(s) 238, 417, 546
BstF5I GGATG 2 cut(s) 13, 258
BstKTI GATC 2 cut(s) 107, 120
BstMAI GTCTC 2 cut(s) 306, 494
BstMBI GATC 2 cut(s) 104, 117
BstSFI CTRYAG 1 cut(s) 616
BstX2I RGATCY 1 cut(s) 117
BstXI CCANNNNNNTGG 1 cut(s) 445
BstYI RGATCY 1 cut(s) 117
BsuRI GGCC 4 cut(s) 344, 398, 506, 593
BtsCI GGATG 2 cut(s) 13, 258
BtsI GCAGTG 1 cut(s) 540
BtsIMutI CAGTG 4 cut(s) 246, 402, 540, 557
Cac8I GCNNGC 1 cut(s) 508
CaiI CAGNNNCTG 1 cut(s) 416
CciI TCATGA 1 cut(s) 478
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 397
Csp6I GTAC 1 cut(s) 79
CviAII CATG 4 cut(s) 111, 169, 479, 589
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 3 cut(s) 238, 417, 546
DpnI GATC 2 cut(s) 106, 119
DpnII GATC 2 cut(s) 104, 117
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
Eco32I GATATC 2 cut(s) 213, 232
Eco57I CTGAAG 1 cut(s) 255
EcoO109I RGGNCCY 1 cut(s) 397
EcoRV GATATC 2 cut(s) 213, 232
Esp3I CGTCTC 1 cut(s) 494
FaeI CATG 4 cut(s) 114, 172, 482, 592
FatI CATG 4 cut(s) 110, 168, 478, 588
Fnu4HI GCNGC 1 cut(s) 453
FokI GGATG 2 cut(s) 20, 245
Fsp4HI GCNGC 1 cut(s) 453
GluI GCNGC 1 cut(s) 453
HaeIII GGCC 4 cut(s) 344, 398, 506, 593
HapII CCGG 2 cut(s) 345, 497
Hin1II CATG 4 cut(s) 114, 172, 482, 592
HinfI GANTC 2 cut(s) 203, 281
HpaII CCGG 2 cut(s) 345, 497
HphI GGTGA 2 cut(s) 290, 359
Hpy166II GTNNAC 1 cut(s) 627
Hpy188I TCNGA 3 cut(s) 102, 208, 274
Hpy188III TCNNGA 4 cut(s) 32, 479, 487, 497
Hpy8I GTNNAC 1 cut(s) 627
HpyAV CCTTC 2 cut(s) 273, 530
HpyCH4III ACNGT 3 cut(s) 289, 375, 406
HpyCH4V TGCA 5 cut(s) 11, 26, 292, 410, 460
HpyF3I CTNAG 3 cut(s) 238, 417, 546
Hsp92II CATG 4 cut(s) 114, 172, 482, 592
Kpn2I TCCGGA 1 cut(s) 496
Kzo9I GATC 2 cut(s) 104, 117
LpnPI CCDG 4 cut(s) 358, 500, 510, 520
LweI GCATC 2 cut(s) 85, 184
MalI GATC 2 cut(s) 106, 119
MboI GATC 2 cut(s) 104, 117
MboII GAAGA 3 cut(s) 28, 65, 391
MflI RGATCY 1 cut(s) 117
MluCI AATT 2 cut(s) 41, 216
MnlI CCTC 9 cut(s) 77, 108, 136, 352, 355, 410, 435, 526, 596
MroI TCCGGA 1 cut(s) 496
MseI TTAA 4 cut(s) 369, 552, 605, 646
MslI CAYNNNNRTG 1 cut(s) 239
MspI CCGG 2 cut(s) 345, 497
NdeII GATC 2 cut(s) 104, 117
NlaIII CATG 4 cut(s) 114, 172, 482, 592
PagI TCATGA 1 cut(s) 478
PcsI WCGNNNNNNNCGW 1 cut(s) 146
PfeI GAWTC 2 cut(s) 203, 281
PkrI GCNGC 1 cut(s) 454
PspPI GGNCC 1 cut(s) 397
PstNI CAGNNNCTG 1 cut(s) 416
PsuI RGATCY 1 cut(s) 117
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
RseI CAYNNNNRTG 1 cut(s) 239
SaqAI TTAA 4 cut(s) 369, 552, 605, 646
SatI GCNGC 1 cut(s) 453
Sau3AI GATC 2 cut(s) 104, 117
Sau96I GGNCC 1 cut(s) 397
SetI ASST 7 cut(s) 65, 69, 147, 326, 418, 529, 623
SfaNI GCATC 2 cut(s) 85, 184
SfcI CTRYAG 1 cut(s) 616
SmiMI CAYNNNNRTG 1 cut(s) 239
Sse9I AATT 2 cut(s) 41, 216
SsiI CCGC 1 cut(s) 452
SspI AATATT 1 cut(s) 163
TaaI ACNGT 3 cut(s) 289, 375, 406
TaqI TCGA 2 cut(s) 266, 377
TasI AATT 2 cut(s) 41, 216
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 2 cut(s) 203, 281
Tru1I TTAA 4 cut(s) 369, 552, 605, 646
Tru9I TTAA 4 cut(s) 369, 552, 605, 646
TscAI CASTG 4 cut(s) 246, 409, 547, 564
TspDTI ATGAA 4 cut(s) 29, 185, 320, 582
TspGWI ACGGA 1 cut(s) 155
TspRI CASTG 4 cut(s) 246, 409, 547, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.