Rorug05G0083400

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000364
Physical Location & Seq
Forward (+)
7220350 .. 7224560
4211 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0083400.1

Sequence Viewer

Length: 1140 bp
ATGGCTGATGATAAGGAAATGGCTGCGTCTGTTGTAGATGGTAGCGATCCAACTGGTCACATAATTTCTACAACCATAGGAGGCAAAAATGGAGAGCCCAAACAGACCATTAGTTATATGGCAGAGCGTGTTGTGGGAACTGGGTCATTTGGAATTGTTTTCCAGGCGAAATGCTTGGAAACTGGTGAGACTGTGGCTATAAAGAAGGTTTTGCAAGACAGAAGATATAAGAACAGGGAACTTCAATTGATGCGTGTGATGGATCATCCAAATGTGATTTGTTTGAAGCATTGCTTCTTCTCTACAACAAGCAAAAATGAGCTTTTTCTCAATTTGGTCATGGAATATGTTCCGGAGACCATGTATCGGGTCTTGAAGCATTACAGCAATGCAAACCAGAGAATGCCCCTTGTATATGTCAAGCTTTACATGTACCAGATTTTCAGAGGACTGGCTTACATACACACCGTTCCTGGAGTTTGCCATAGAGATTTGAAGCCTCAAAATTTATTGGTTGATCCTCTAACTCACCAGGTCAAGCTCTGTGATTTTGGAAGTGCCAAAATGCTGGTGAAAGGTGAAGCAAACATAGCATACATATGTTCACGTTTCTATCGGGCTCCAGAACTAATATTTGGTGCCACGGATTATACAACTTCAATTGATATCTGGTCAGCTGGCTGTGTCCTAGCAGAGCTTCTTTTGGGCCAGCCTTTGTTTCCTGGGGAAAATGCAGTGGACCAACTTGTAGAGATCATCAAGGTTCTGGGAACACCAACACGTGAAGAAATTCGCTGTATGAATCCAAACTATACAGATTTCAGGTTTCCACAGATAAAAGCACACCCTTGGCACAAGGTTTTCCACAAGCGCATGCCTCCAGAAGCAATTGATCTGGCTTCAAGACTGCTACAATACTCTCCAAGTCTTCGTTGCACAGCTCTAGAAGCATGTGCACATCCTTTCTTTGACGAACTCCGTGAGTCCAATGCCCGCCTACCAAATGGTCGCCCATTGCCTCCGCTTTTTAATTTTAAGCAAGAGTTGTCCGGCGCTTCTCCAGAGCTTATCAACAAGTTGATTCCTGATCATGTGAAACGCCAAATGGGTCTCCCTTTCTTGCATGCGGCTGGCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

379

Amino Acids

42.84

Weight (kDa)

8.58

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 40 - 323 1.2e-61 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 42 - 243 1.3e-24 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 638
AccIII TCCGGA 1 cut(s) 352
AciI CCGC 3 cut(s) 994, 1022, 1127
AclWI GGATC 3 cut(s) 41, 270, 512
AcsI RAATTY 2 cut(s) 505, 789
AcvI CACGTG 1 cut(s) 782
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 1 cut(s) 366
AflIII ACRYGT 2 cut(s) 429, 779
AgsI TTSAA 6 cut(s) 245, 286, 376, 496, 660, 903
AjnI CCWGG 4 cut(s) 162, 472, 531, 721
AjuI GAANNNNNNNTTGG 2 cut(s) 618, 650
AluBI AGCT 7 cut(s) 322, 424, 541, 677, 697, 941, 1066
AluI AGCT 7 cut(s) 322, 424, 541, 677, 697, 941, 1066
Alw21I GWGCWC 1 cut(s) 958
Alw26I GTCTC 3 cut(s) 182, 350, 1115
Alw44I GTGCAC 1 cut(s) 954
AlwI GGATC 3 cut(s) 41, 270, 512
Aor13HI TCCGGA 1 cut(s) 352
AoxI GGCC 1 cut(s) 706
ApaLI GTGCAC 1 cut(s) 954
ApeKI GCWGC 1 cut(s) 23
ApoI RAATTY 2 cut(s) 505, 789
Asp700I GAANNNNTTC 2 cut(s) 348, 789
AspLEI GCGC 2 cut(s) 873, 1055
AspS9I GGNCC 2 cut(s) 706, 739
AsuHPI GGTGA 4 cut(s) 197, 521, 583, 590
AvaII GGWCC 1 cut(s) 739
BaeGI GKGCMC 1 cut(s) 958
BanI GGYRCC 1 cut(s) 638
BanII GRGCYC 2 cut(s) 99, 622
BbrPI CACGTG 1 cut(s) 782
BbsI GAAGAC 1 cut(s) 920
Bbv12I GWGCWC 1 cut(s) 958
BbvI GCAGC 1 cut(s) 10
BccI CCATC 2 cut(s) 32, 253
BciT130I CCWGG 4 cut(s) 164, 474, 533, 723
BclI TGATCA 1 cut(s) 1087
BcoDI GTCTC 3 cut(s) 182, 350, 1115
BfaI CTAG 2 cut(s) 689, 944
BfoI RGCGCY 1 cut(s) 1056
BisI GCNGC 2 cut(s) 24, 1128
BlsI GCNGC 2 cut(s) 25, 1129
Bme1390I CCNGG 4 cut(s) 164, 474, 533, 723
Bme18I GGWCC 1 cut(s) 739
BmgT120I GGNCC 2 cut(s) 706, 739
BmiI GGNNCC 2 cut(s) 621, 640
BmrFI CCNGG 4 cut(s) 164, 474, 533, 723
BmrI ACTGGG 1 cut(s) 150
BmsI GCATC 1 cut(s) 240
BmuI ACTGGG 1 cut(s) 150
BpiI GAAGAC 1 cut(s) 920
BpmI CTGGAG 4 cut(s) 495, 606, 864, 1044
BsaAI YACGTR 1 cut(s) 782
BsaI GGTCTC 2 cut(s) 350, 1115
BsaJI CCNNGG 3 cut(s) 642, 722, 848
BsaWI WCCGGW 1 cut(s) 352
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse1I ACTGG 4 cut(s) 58, 145, 187, 456
Bse3DI GCAATG 3 cut(s) 289, 394, 1013
BseAI TCCGGA 1 cut(s) 352
BseBI CCWGG 4 cut(s) 164, 474, 533, 723
BseDI CCNNGG 3 cut(s) 642, 722, 848
BseGI GGATG 2 cut(s) 265, 958
BseLI CCNNNNNNNGG 1 cut(s) 366
BseMI GCAATG 3 cut(s) 289, 394, 1013
BseNI ACTGG 4 cut(s) 58, 145, 187, 456
BseSI GKGCMC 1 cut(s) 958
BseXI GCAGC 1 cut(s) 10
BshFI GGCC 1 cut(s) 708
BshNI GGYRCC 1 cut(s) 638
BsiHKAI GWGCWC 1 cut(s) 958
BsiSI CCGG 2 cut(s) 353, 1050
BslI CCNNNNNNNGG 1 cut(s) 366
BsmAI GTCTC 3 cut(s) 182, 350, 1115
BsmI GAATGC 1 cut(s) 408
BsnI GGCC 1 cut(s) 708
Bso31I GGTCTC 2 cut(s) 350, 1115
Bsp1286I GDGCHC 3 cut(s) 99, 622, 958
Bsp13I TCCGGA 1 cut(s) 352
Bsp143I GATC 6 cut(s) 46, 262, 517, 753, 892, 1087
BspACI CCGC 3 cut(s) 994, 1022, 1127
BspANI GGCC 1 cut(s) 708
BspEI TCCGGA 1 cut(s) 352
BspLI GGNNCC 2 cut(s) 621, 640
BspPI GGATC 3 cut(s) 41, 270, 512
BspT107I GGYRCC 1 cut(s) 638
BspTNI GGTCTC 2 cut(s) 350, 1115
BsrDI GCAATG 3 cut(s) 289, 394, 1013
BsrI ACTGG 4 cut(s) 58, 145, 187, 456
BssECI CCNNGG 3 cut(s) 642, 722, 848
BssMI GATC 6 cut(s) 46, 262, 517, 753, 892, 1087
BssT1I CCWWGG 1 cut(s) 848
Bst2UI CCWGG 4 cut(s) 164, 474, 533, 723
Bst4CI ACNGT 2 cut(s) 193, 469
BstBAI YACGTR 1 cut(s) 782
BstC8I GCNNGC 6 cut(s) 679, 710, 875, 994, 1125, 1132
BstDSI CCRYGG 1 cut(s) 642
BstF5I GGATG 2 cut(s) 265, 958
BstH2I RGCGCY 1 cut(s) 1056
BstHHI GCGC 2 cut(s) 873, 1055
BstKTI GATC 6 cut(s) 49, 265, 520, 756, 895, 1090
BstMAI GTCTC 3 cut(s) 182, 350, 1115
BstMBI GATC 6 cut(s) 46, 262, 517, 753, 892, 1087
BstMWI GCNNNNNNNGC 2 cut(s) 590, 947
BstNI CCWGG 4 cut(s) 164, 474, 533, 723
BstNSI RCATGY 4 cut(s) 433, 877, 954, 1127
BstSCI CCNGG 4 cut(s) 162, 472, 531, 721
BstSLI GKGCMC 1 cut(s) 958
BstV1I GCAGC 1 cut(s) 10
BstV2I GAAGAC 1 cut(s) 920
BstXI CCANNNNNNTGG 1 cut(s) 568
BsuRI GGCC 1 cut(s) 708
BtgI CCRYGG 1 cut(s) 642
BtsCI GGATG 2 cut(s) 265, 958
BtsI GCAGTG 1 cut(s) 741
BtsIMutI CAGTG 1 cut(s) 741
Cac8I GCNNGC 6 cut(s) 679, 710, 875, 994, 1125, 1132
CfoI GCGC 2 cut(s) 873, 1055
Cfr13I GGNCC 2 cut(s) 706, 739
CseI GACGC 1 cut(s) 15
CsiI ACCWGGT 1 cut(s) 531
Csp6I GTAC 1 cut(s) 433
CviAII CATG 7 cut(s) 340, 361, 430, 874, 951, 1091, 1124
CviQI GTAC 1 cut(s) 433
DpnI GATC 6 cut(s) 48, 264, 519, 755, 894, 1089
DpnII GATC 6 cut(s) 46, 262, 517, 753, 892, 1087
Eco130I CCWWGG 1 cut(s) 848
Eco24I GRGCYC 2 cut(s) 99, 622
Eco31I GGTCTC 2 cut(s) 350, 1115
Eco32I GATATC 1 cut(s) 667
Eco47I GGWCC 1 cut(s) 739
Eco72I CACGTG 1 cut(s) 782
EcoRII CCWGG 4 cut(s) 162, 472, 531, 721
EcoRV GATATC 1 cut(s) 667
EcoT14I CCWWGG 1 cut(s) 848
EcoT38I GRGCYC 2 cut(s) 99, 622
ErhI CCWWGG 1 cut(s) 848
FaeI CATG 7 cut(s) 343, 364, 433, 877, 954, 1094, 1127
FalI AAGNNNNNCTT 2 cut(s) 278, 310
FatI CATG 7 cut(s) 339, 360, 429, 873, 950, 1090, 1123
FauI CCCGC 1 cut(s) 1001
FauNDI CATATG 1 cut(s) 599
FbaI TGATCA 1 cut(s) 1087
Fnu4HI GCNGC 2 cut(s) 24, 1128
FokI GGATG 2 cut(s) 252, 945
FriOI GRGCYC 2 cut(s) 99, 622
Fsp4HI GCNGC 2 cut(s) 24, 1128
FspBI CTAG 2 cut(s) 689, 944
GlaI GCGC 2 cut(s) 872, 1054
GluI GCNGC 2 cut(s) 24, 1128
GsuI CTGGAG 4 cut(s) 495, 606, 864, 1044
HaeII RGCGCY 1 cut(s) 1056
HaeIII GGCC 1 cut(s) 708
HapII CCGG 2 cut(s) 353, 1050
HgaI GACGC 1 cut(s) 15
HhaI GCGC 2 cut(s) 873, 1055
Hin1II CATG 7 cut(s) 343, 364, 433, 877, 954, 1094, 1127
Hin6I GCGC 2 cut(s) 871, 1053
HinP1I GCGC 2 cut(s) 871, 1053
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 3 cut(s) 802, 983, 1081
HpaII CCGG 2 cut(s) 353, 1050
HphI GGTGA 4 cut(s) 197, 521, 583, 590
Hpy166II GTNNAC 3 cut(s) 605, 739, 956
Hpy188I TCNGA 1 cut(s) 446
Hpy188III TCNNGA 8 cut(s) 353, 373, 623, 881, 903, 944, 1061, 1085
Hpy8I GTNNAC 3 cut(s) 605, 739, 956
HpyAV CCTTC 1 cut(s) 199
HpyCH4III ACNGT 2 cut(s) 193, 469
HpyCH4IV ACGT 2 cut(s) 607, 781
HpyCH4V TGCA 6 cut(s) 214, 392, 734, 936, 956, 1123
HpyF10VI GCNNNNNNNGC 2 cut(s) 590, 947
HpySE526I ACGT 2 cut(s) 607, 781
Hsp92II CATG 7 cut(s) 343, 364, 433, 877, 954, 1094, 1127
HspAI GCGC 2 cut(s) 871, 1053
Kpn2I TCCGGA 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 1087
Kzo9I GATC 6 cut(s) 46, 262, 517, 753, 892, 1087
LmnI GCTCC 1 cut(s) 625
Lsp1109I GCAGC 1 cut(s) 10
LweI GCATC 1 cut(s) 240
MabI ACCWGGT 1 cut(s) 531
MaeI CTAG 2 cut(s) 689, 944
MaeII ACGT 2 cut(s) 607, 781
MaeIII GTNAC 1 cut(s) 56
MalI GATC 6 cut(s) 48, 264, 519, 755, 894, 1089
MboI GATC 6 cut(s) 46, 262, 517, 753, 892, 1087
MboII GAAGA 4 cut(s) 234, 289, 797, 920
MfeI CAATTG 3 cut(s) 245, 660, 888
MhlI GDGCHC 3 cut(s) 99, 622, 958
MluCI AATT 9 cut(s) 63, 153, 245, 331, 505, 660, 789, 888, 1030
MlyI GAGTC 1 cut(s) 992
MmeI TCCRAC 1 cut(s) 74
MnlI CCTC 6 cut(s) 74, 440, 510, 531, 888, 1029
MroI TCCGGA 1 cut(s) 352
MroXI GAANNNNTTC 2 cut(s) 348, 789
MseI TTAA 2 cut(s) 1029, 1035
MslI CAYNNNNRTG 2 cut(s) 270, 598
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 2 cut(s) 353, 1050
MspR9I CCNGG 4 cut(s) 164, 474, 533, 723
MunI CAATTG 3 cut(s) 245, 660, 888
Mva1269I GAATGC 1 cut(s) 408
MvaI CCWGG 4 cut(s) 164, 474, 533, 723
MwoI GCNNNNNNNGC 2 cut(s) 590, 947
NdeI CATATG 1 cut(s) 599
NdeII GATC 6 cut(s) 46, 262, 517, 753, 892, 1087
NlaIII CATG 7 cut(s) 343, 364, 433, 877, 954, 1094, 1127
NlaIV GGNNCC 2 cut(s) 621, 640
NmuCI GTSAC 1 cut(s) 56
NspI RCATGY 4 cut(s) 433, 877, 954, 1127
PaeI GCATGC 2 cut(s) 877, 1127
PciI ACATGT 1 cut(s) 429
PctI GAATGC 1 cut(s) 408
PdmI GAANNNNTTC 2 cut(s) 348, 789
PfeI GAWTC 2 cut(s) 802, 1081
PfoI TCCNGGA 1 cut(s) 472
PkrI GCNGC 2 cut(s) 25, 1129
PleI GAGTC 1 cut(s) 991
PmaCI CACGTG 1 cut(s) 782
PmlI CACGTG 1 cut(s) 782
PpsI GAGTC 1 cut(s) 991
Ppu21I YACGTR 1 cut(s) 782
PscI ACATGT 1 cut(s) 429
Psp6I CCWGG 4 cut(s) 162, 472, 531, 721
PspCI CACGTG 1 cut(s) 782
PspGI CCWGG 4 cut(s) 162, 472, 531, 721
PspN4I GGNNCC 2 cut(s) 621, 640
PspPI GGNCC 2 cut(s) 706, 739
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 2 cut(s) 270, 598
SaqAI TTAA 2 cut(s) 1029, 1035
SatI GCNGC 2 cut(s) 24, 1128
Sau3AI GATC 6 cut(s) 46, 262, 517, 753, 892, 1087
Sau96I GGNCC 2 cut(s) 706, 739
SchI GAGTC 1 cut(s) 992
ScrFI CCNGG 4 cut(s) 164, 474, 533, 723
SduI GDGCHC 3 cut(s) 99, 622, 958
SexAI ACCWGGT 1 cut(s) 531
SfaNI GCATC 1 cut(s) 240
SinI GGWCC 1 cut(s) 739
SmiMI CAYNNNNRTG 2 cut(s) 270, 598
SphI GCATGC 2 cut(s) 877, 1127
Sse9I AATT 9 cut(s) 63, 153, 245, 331, 505, 660, 789, 888, 1030
SsiI CCGC 3 cut(s) 994, 1022, 1127
SspI AATATT 1 cut(s) 633
SspMI CTAG 2 cut(s) 689, 944
StyD4I CCNGG 4 cut(s) 162, 472, 531, 721
StyI CCWWGG 1 cut(s) 848
TaaI ACNGT 2 cut(s) 193, 469
TaiI ACGT 2 cut(s) 610, 784
TasI AATT 9 cut(s) 63, 153, 245, 331, 505, 660, 789, 888, 1030
TauI GCSGC 1 cut(s) 1130
TfiI GAWTC 2 cut(s) 802, 1081
Tru1I TTAA 2 cut(s) 1029, 1035
Tru9I TTAA 2 cut(s) 1029, 1035
TscAI CASTG 1 cut(s) 741
TseFI GTSAC 1 cut(s) 56
TseI GCWGC 1 cut(s) 23
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 1 cut(s) 815
TspGWI ACGGA 2 cut(s) 659, 968
TspRI CASTG 1 cut(s) 741
VneI GTGCAC 1 cut(s) 954
VpaK11BI GGWCC 1 cut(s) 739
XapI RAATTY 2 cut(s) 505, 789
XbaI TCTAGA 1 cut(s) 943
XceI RCATGY 4 cut(s) 433, 877, 954, 1127
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XmnI GAANNNNTTC 2 cut(s) 348, 789
XspI CTAG 2 cut(s) 689, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.