Rh5AG174300

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000364
Physical Location & Seq
Forward (+)
19262062 .. 19263436
1375 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG174300.1

Sequence Viewer

Length: 693 bp
ATGACGACTAAGAAGAACAAGAAATCTCAAGCTAAAAAGATCGATCAAGAGCAATTGCATGGCTTTGAGAAATGGATTAAGCACTACAGCAGCAACCAAACCATACTGCAAGTCGGAGAAGGAGACTTCTCGTTTGCTCTTTGCATAGCTAGACAATTTGGCTCTGGTAGGAACATGATTGCAACTTCTCTCGACTCCAAAGGGGAGTTGATCAAATATTCAAAAGCTATGAGCAATTTGAGGGAGTTAGAGAGCAGAGGATGCCAAATTTTGCACGGGGTAGATGTCAACACCATGCTCCATCACCCTGAACTTATTACTAAACAGTTTGATCGAATAATTTATAACTTTCCTCATGCTGGTTACTTGAAAGGCCTCCGTCGTTCATCAGAAAAGAATAAGTCCCAAATTTTGTTGCATCAGATTTTGGTGAGGGGATACTTCATGAACTCACGGGAGATGCTGACTAAAAATGGAGAAATTCATGTCACACACAAGACAACATATCCTTTTAGTGAGTGGGAAATAGTGGAGTTAGCAGAGGAGGCTGAGTTATTCTTGGTTAATGAAGAAGAGTTTTACAAACTGGATTATCCGGGATATGAAAACAAGAGAGGAGATGGGATTTGCGACGAGTCATTCCCGGTTGGGAAGTCTAGCACCTTCATCTTTGCCAAGCGGTTCGTACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

230

Amino Acids

26.61

Weight (kDa)

8.61

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 36 - 205 2.6e-49 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
AciI CCGC 1 cut(s) 679
AcsI RAATTY 3 cut(s) 267, 408, 480
AfaI GTAC 1 cut(s) 687
AfiI CCNNNNNNNGG 1 cut(s) 359
AgsI TTSAA 2 cut(s) 222, 370
AluBI AGCT 3 cut(s) 32, 149, 227
AluI AGCT 3 cut(s) 32, 149, 227
Alw26I GTCTC 1 cut(s) 117
AoxI GGCC 1 cut(s) 373
ApeKI GCWGC 1 cut(s) 90
ApoI RAATTY 3 cut(s) 267, 408, 480
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
AsuC2I CCSGG 2 cut(s) 597, 644
AsuHPI GGTGA 2 cut(s) 296, 442
BbvI GCAGC 1 cut(s) 102
BccI CCATC 2 cut(s) 309, 614
BciVI GTATCC 1 cut(s) 431
BclI TGATCA 1 cut(s) 210
BcnI CCSGG 2 cut(s) 597, 644
BcoDI GTCTC 1 cut(s) 117
BfaI CTAG 2 cut(s) 150, 657
BfmI CTRYAG 1 cut(s) 85
BfuI GTATCC 1 cut(s) 431
BisI GCNGC 1 cut(s) 91
BlsI GCNGC 1 cut(s) 92
Bme1390I CCNGG 2 cut(s) 597, 644
BmrFI CCNGG 2 cut(s) 597, 644
BmsI GCATC 3 cut(s) 251, 427, 450
BpuEI CTTGAG 1 cut(s) 12
BpuMI CCSGG 2 cut(s) 597, 644
Bsa29I ATCGAT 1 cut(s) 42
BsaXI ACNNNNNCTCC 2 cut(s) 536, 566
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse1I ACTGG 1 cut(s) 591
BseCI ATCGAT 1 cut(s) 42
BseGI GGATG 1 cut(s) 266
BseLI CCNNNNNNNGG 1 cut(s) 359
BseMII CTCAG 1 cut(s) 540
BseNI ACTGG 1 cut(s) 591
BseRI GAGGAG 2 cut(s) 557, 630
BseXI GCAGC 1 cut(s) 102
BshFI GGCC 1 cut(s) 375
BshVI ATCGAT 1 cut(s) 42
BsiSI CCGG 2 cut(s) 596, 644
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 1 cut(s) 359
BsmAI GTCTC 1 cut(s) 117
BsmFI GGGAC 1 cut(s) 388
BsnI GGCC 1 cut(s) 375
Bsp143I GATC 4 cut(s) 39, 43, 210, 331
BspACI CCGC 1 cut(s) 679
BspANI GGCC 1 cut(s) 375
BspCNI CTCAG 1 cut(s) 541
BspDI ATCGAT 1 cut(s) 42
BspHI TCATGA 1 cut(s) 444
BsrI ACTGG 1 cut(s) 591
BssMI GATC 4 cut(s) 39, 43, 210, 331
Bst4CI ACNGT 1 cut(s) 327
Bst6I CTCTTC 1 cut(s) 567
BstAPI GCANNNNNTGC 1 cut(s) 261
BstDEI CTNAG 2 cut(s) 9, 549
BstF5I GGATG 1 cut(s) 266
BstKTI GATC 4 cut(s) 42, 46, 213, 334
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 4 cut(s) 39, 43, 210, 331
BstMWI GCNNNNNNNGC 2 cut(s) 261, 545
BstSCI CCNGG 2 cut(s) 595, 642
BstSFI CTRYAG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 102
Bsu15I ATCGAT 1 cut(s) 42
BsuI GTATCC 1 cut(s) 431
BsuRI GGCC 1 cut(s) 375
BsuTUI ATCGAT 1 cut(s) 42
BtsCI GGATG 1 cut(s) 266
CciI TCATGA 1 cut(s) 444
ClaI ATCGAT 1 cut(s) 42
Csp6I GTAC 1 cut(s) 686
CviAII CATG 6 cut(s) 59, 175, 295, 356, 445, 485
CviJI RGCY 7 cut(s) 32, 63, 149, 162, 227, 375, 548
CviKI_1 RGCY 7 cut(s) 32, 63, 149, 162, 227, 375, 548
CviQI GTAC 1 cut(s) 686
DdeI CTNAG 2 cut(s) 9, 549
DpnI GATC 4 cut(s) 41, 45, 212, 333
DpnII GATC 4 cut(s) 39, 43, 210, 331
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
Eco147I AGGCCT 1 cut(s) 375
FaeI CATG 6 cut(s) 62, 178, 298, 359, 448, 488
FaqI GGGAC 1 cut(s) 388
FatI CATG 6 cut(s) 58, 174, 294, 355, 444, 484
FbaI TGATCA 1 cut(s) 210
Fnu4HI GCNGC 1 cut(s) 91
FokI GGATG 1 cut(s) 273
Fsp4HI GCNGC 1 cut(s) 91
FspBI CTAG 2 cut(s) 150, 657
GluI GCNGC 1 cut(s) 91
HaeIII GGCC 1 cut(s) 375
HapII CCGG 2 cut(s) 596, 644
Hin1II CATG 6 cut(s) 62, 178, 298, 359, 448, 488
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 2 cut(s) 194, 635
HpaII CCGG 2 cut(s) 596, 644
HphI GGTGA 2 cut(s) 296, 442
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 3 cut(s) 116, 391, 423
Hpy188III TCNNGA 3 cut(s) 47, 191, 445
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 2 cut(s) 384, 635
HpyAV CCTTC 2 cut(s) 113, 673
HpyCH4III ACNGT 1 cut(s) 327
HpyCH4V TGCA 6 cut(s) 58, 109, 144, 182, 274, 418
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 545
HpyF3I CTNAG 2 cut(s) 9, 549
Hsp92II CATG 6 cut(s) 62, 178, 298, 359, 448, 488
Ksp22I TGATCA 1 cut(s) 210
Kzo9I GATC 4 cut(s) 39, 43, 210, 331
LmnI GCTCC 1 cut(s) 303
LpnPI CCDG 6 cut(s) 150, 321, 345, 572, 609, 657
Lsp1109I GCAGC 1 cut(s) 102
LweI GCATC 3 cut(s) 251, 427, 450
MaeI CTAG 2 cut(s) 150, 657
MaeIII GTNAC 2 cut(s) 362, 487
MalI GATC 4 cut(s) 41, 45, 212, 333
MboI GATC 4 cut(s) 39, 43, 210, 331
MboII GAAGA 3 cut(s) 25, 581, 584
MfeI CAATTG 1 cut(s) 53
MluCI AATT 7 cut(s) 53, 155, 235, 267, 339, 408, 480
MlyI GAGTC 2 cut(s) 188, 644
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 8 cut(s) 234, 251, 363, 386, 426, 535, 538, 608
MseI TTAA 2 cut(s) 78, 564
MspI CCGG 2 cut(s) 596, 644
MspR9I CCNGG 2 cut(s) 597, 644
MunI CAATTG 1 cut(s) 53
MwoI GCNNNNNNNGC 2 cut(s) 261, 545
NciI CCSGG 2 cut(s) 597, 644
NdeII GATC 4 cut(s) 39, 43, 210, 331
NlaIII CATG 6 cut(s) 62, 178, 298, 359, 448, 488
NmuCI GTSAC 1 cut(s) 487
PagI TCATGA 1 cut(s) 444
PceI AGGCCT 1 cut(s) 375
PfoI TCCNGGA 1 cut(s) 595
PkrI GCNGC 1 cut(s) 92
PleI GAGTC 2 cut(s) 188, 643
PpsI GAGTC 2 cut(s) 188, 643
PsiI TTATAA 1 cut(s) 345
RsaI GTAC 1 cut(s) 687
RsaNI GTAC 1 cut(s) 686
SaqAI TTAA 2 cut(s) 78, 564
SatI GCNGC 1 cut(s) 91
Sau3AI GATC 4 cut(s) 39, 43, 210, 331
SchI GAGTC 2 cut(s) 188, 644
ScrFI CCNGG 2 cut(s) 597, 644
SetI ASST 4 cut(s) 34, 151, 229, 665
SfaNI GCATC 3 cut(s) 251, 427, 450
SfcI CTRYAG 1 cut(s) 85
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
Sse9I AATT 7 cut(s) 53, 155, 235, 267, 339, 408, 480
SseBI AGGCCT 1 cut(s) 375
SsiI CCGC 1 cut(s) 679
SspI AATATT 1 cut(s) 218
SspMI CTAG 2 cut(s) 150, 657
StuI AGGCCT 1 cut(s) 375
StyD4I CCNGG 2 cut(s) 595, 642
TaaI ACNGT 1 cut(s) 327
TaqI TCGA 3 cut(s) 42, 192, 334
TasI AATT 7 cut(s) 53, 155, 235, 267, 339, 408, 480
Tru1I TTAA 2 cut(s) 78, 564
Tru9I TTAA 2 cut(s) 78, 564
TseFI GTSAC 1 cut(s) 487
TseI GCWGC 1 cut(s) 90
Tsp45I GTSAC 1 cut(s) 487
TspDTI ATGAA 7 cut(s) 375, 433, 461, 473, 582, 618, 655
TspGWI ACGGA 1 cut(s) 368
XapI RAATTY 3 cut(s) 267, 408, 480
XspI CTAG 2 cut(s) 150, 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.