FvH4_3g14862

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000364
Physical Location & Seq
Forward (+)
9184734 .. 9185974
1241 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g14862.t2

Sequence Viewer

Length: 429 bp
ATGAGGCAGCACCCTCTCCTACTCGATCAACGGTTTGATCGTATAGTCTTTAACTTTCCTCATGCTGGTTTCATTTTCCCTCATGCTGGTTTCATTTTTATGGAAACCCAGAAGAGCCAGATCAAGTTGCATCAGGATTTGGTGAGGACATTCTTCAACAGCGCATGTGAGATGTTGAAAGGAAGTGGAGAAGTTCATGTCACTCACAAGACCTCACATCCTTTCAGTAAATGGGAGAGAGTGAAATTAGCAGAAGAGGTTGGGTTATATCTGGTTGAGGAAGCCTCGTTTTCCAGAGGGGATTATCCAGGTTATCTGAACAAGAGAGGGAGTGGGAAGAAATGTAATCGCACTTTCCCTATTGGTCTATGCAGTACCTACAAATTTGCCAAGCTACCACTTCTTGAATTCTCACAGACAACACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

143

Amino Acids

16.4

Weight (kDa)

9.38

Isoelectric Point (pI)

28.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 5 - 109 1.8e-25 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 383, 407
AfaI GTAC 1 cut(s) 376
AfiI CCNNNNNNNGG 2 cut(s) 65, 86
AgsI TTSAA 3 cut(s) 157, 178, 407
AjnI CCWGG 1 cut(s) 307
AluBI AGCT 1 cut(s) 394
AluI AGCT 1 cut(s) 394
ApeKI GCWGC 1 cut(s) 7
ApoI RAATTY 2 cut(s) 383, 407
AspLEI GCGC 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 154
BbvI GCAGC 1 cut(s) 19
BciT130I CCWGG 1 cut(s) 309
BfmI CTRYAG 1 cut(s) 425
BisI GCNGC 1 cut(s) 8
BlsI GCNGC 1 cut(s) 9
Bme1390I CCNGG 1 cut(s) 309
BmrFI CCNGG 1 cut(s) 309
BmsI GCATC 1 cut(s) 139
BplI GAGNNNNNCTC 2 cut(s) 269, 301
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 86
BseBI CCWGG 1 cut(s) 309
BseGI GGATG 1 cut(s) 217
BseLI CCNNNNNNNGG 2 cut(s) 65, 86
BseXI GCAGC 1 cut(s) 19
BslI CCNNNNNNNGG 2 cut(s) 65, 86
Bsp143I GATC 3 cut(s) 25, 37, 120
BspQI GCTCTTC 1 cut(s) 107
BssMI GATC 3 cut(s) 25, 37, 120
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 1 cut(s) 33
Bst6I CTCTTC 2 cut(s) 107, 249
BstF5I GGATG 1 cut(s) 217
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 3 cut(s) 28, 40, 123
BstMBI GATC 3 cut(s) 25, 37, 120
BstNI CCWGG 1 cut(s) 309
BstNSI RCATGY 1 cut(s) 168
BstSCI CCNGG 1 cut(s) 307
BstSFI CTRYAG 1 cut(s) 425
BstV1I GCAGC 1 cut(s) 19
BtsCI GGATG 1 cut(s) 217
CfoI GCGC 1 cut(s) 164
Csp6I GTAC 1 cut(s) 375
CviAII CATG 4 cut(s) 62, 83, 165, 197
CviJI RGCY 3 cut(s) 117, 284, 394
CviKI_1 RGCY 3 cut(s) 117, 284, 394
CviQI GTAC 1 cut(s) 375
DpnI GATC 3 cut(s) 27, 39, 122
DpnII GATC 3 cut(s) 25, 37, 120
Eam1104I CTCTTC 2 cut(s) 107, 249
EarI CTCTTC 2 cut(s) 107, 249
EcoRI GAATTC 1 cut(s) 407
EcoRII CCWGG 1 cut(s) 307
FaeI CATG 4 cut(s) 65, 86, 168, 200
FaiI YATR 9 cut(s) 44, 63, 84, 101, 166, 198, 268, 370, 427
FatI CATG 4 cut(s) 61, 82, 164, 196
Fnu4HI GCNGC 1 cut(s) 8
FokI GGATG 1 cut(s) 204
Fsp4HI GCNGC 1 cut(s) 8
GlaI GCGC 1 cut(s) 163
GluI GCNGC 1 cut(s) 8
HhaI GCGC 1 cut(s) 164
Hin1II CATG 4 cut(s) 65, 86, 168, 200
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HphI GGTGA 1 cut(s) 154
Hpy188I TCNGA 1 cut(s) 318
Hpy188III TCNNGA 3 cut(s) 134, 294, 404
HpyCH4III ACNGT 1 cut(s) 33
HpyCH4V TGCA 2 cut(s) 130, 372
Hsp92II CATG 4 cut(s) 65, 86, 168, 200
HspAI GCGC 1 cut(s) 162
Kzo9I GATC 3 cut(s) 25, 37, 120
LguI GCTCTTC 1 cut(s) 107
LpnPI CCDG 9 cut(s) 51, 72, 119, 122, 131, 257, 294, 307, 321
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 1 cut(s) 139
MaeIII GTNAC 1 cut(s) 199
MalI GATC 3 cut(s) 27, 39, 122
MboI GATC 3 cut(s) 25, 37, 120
MboII GAAGA 4 cut(s) 124, 145, 266, 349
MluCI AATT 3 cut(s) 245, 383, 407
MseI TTAA 1 cut(s) 51
MslI CAYNNNNRTG 1 cut(s) 98
MspR9I CCNGG 1 cut(s) 309
MvaI CCWGG 1 cut(s) 309
NdeII GATC 3 cut(s) 25, 37, 120
NlaIII CATG 4 cut(s) 65, 86, 168, 200
NmuCI GTSAC 1 cut(s) 199
NspI RCATGY 1 cut(s) 168
PciSI GCTCTTC 1 cut(s) 107
PcsI WCGNNNNNNNCGW 1 cut(s) 37
PkrI GCNGC 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 307
PspGI CCWGG 1 cut(s) 307
RsaI GTAC 1 cut(s) 376
RsaNI GTAC 1 cut(s) 375
RseI CAYNNNNRTG 1 cut(s) 98
SapI GCTCTTC 1 cut(s) 107
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 1 cut(s) 8
Sau3AI GATC 3 cut(s) 25, 37, 120
ScrFI CCNGG 1 cut(s) 309
SetI ASST 5 cut(s) 215, 261, 313, 380, 396
SfaNI GCATC 1 cut(s) 139
SfcI CTRYAG 1 cut(s) 425
SmiMI CAYNNNNRTG 1 cut(s) 98
Sse9I AATT 3 cut(s) 245, 383, 407
StyD4I CCNGG 1 cut(s) 307
TaaI ACNGT 1 cut(s) 33
TaqI TCGA 1 cut(s) 24
TasI AATT 3 cut(s) 245, 383, 407
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 1 cut(s) 7
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 3 cut(s) 61, 82, 185
XapI RAATTY 2 cut(s) 383, 407
XceI RCATGY 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.