Rh5DG174100

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000364
Physical Location & Seq
Forward (+)
18686730 .. 18689849
3120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG174100.1

Sequence Viewer

Length: 1149 bp
ATGGAAGAGGCTATAGCAGATACTAAACGAGAGAAAAGGATTATGCATTACAGCAGCAATCAGAAGATACTGTTAGTGGGTGAGGGCAACTTCTCTTTTGCTGCTTGTTTAGCCAAAGAGTTTGGTTCAGCGAAGAACATGGTTGCTTCTTCTCTTGACTCCAAAGAGTCAGTAATGATTAGATACTCAAATGCAGCACCAAGCAACTTGAAGGAATTAGAGGACATGGGATGTGTAATCTTGCATGAGGTGGATGTCCACACCATGAGACAGCACCCTCTTCTAGTTAATCAACTGTTTGATCGCGTAGTCTTTAACTTTCCTCATGCTGGCTTTATTTTCATGGAAAATCAGATGAGCCAGATCAAGTTGCATCAGGATTTAATGAGAGGATTCTTCACCAGTGCATGTGAAATGCTGAAAGAAAGTGGAGAAGTTCATGTGACTCACAAGACATCACATCCATTCAGTAAGTGGGAGATAGTGAAATTAGCAGAAGAGACCGGGTTATATCTGGTTGAGGAAGCATCGTTTACAAGAGGGGATTATCCTGTATTTGGCTCTGCTAAGAACATGGTTGCCACTTCCCGCGAATCCAGAGAGTCAGTATTGGCCCAGTATTCAGATGCAGCACCAAGAAACTTGACAGAATTGGAGTACATGGGATGTGATATACTGCATGAGGTGGATGTACACACCATGAGGCAACACCCTTTCTTAATCGATCAACTGTTTGATCGGATAGTCTTCAACTTTCCTCATGCTGGTTTCGTTTTTATGGAAAGCAAGAAGAAGAAAACCAAGTATTTTATGGAAAACAGCAAGAGGCAAATCAAGTTGCATCAGGATTTGGTGAGGAGATTCTTCGCCAGCGCATGTGAGATGCTGAAAGAAAGTGGAGAAGTTCATGTCACTCACAAGACCGCATATCCATTCAGTGAGTGGGAGATAGTGAAATTAGCAGGAGAGGCTGGGTTATATCTGGTTGAGGAAGCATCGTTTTCAAGAGAGGATTATCCCGGTTATTTAAACAAGAGAGGAAGTGGGAAGAAATGCAACCGCACATTTCCTGTTGGACAATGTAGCACCTACAAATTTGCCAAGCTGCCGCTTCTTGAATTTTCATTCACAACAATAAAGATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

382

Amino Acids

43.82

Weight (kDa)

6.77

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 24 - 187 1.8e-50 rRNA (uridine-N3-)-methyltransferase BTM5-like
BMT5-like PF10354 187 - 346 2.3e-39 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 306, 591
AciI CCGC 4 cut(s) 589, 924, 1060, 1109
AcsI RAATTY 2 cut(s) 1094, 1118
AfaI GTAC 2 cut(s) 659, 693
AfiI CCNNNNNNNGG 3 cut(s) 329, 557, 764
AgsI TTSAA 4 cut(s) 211, 751, 1005, 1118
AluBI AGCT 1 cut(s) 1105
AluI AGCT 1 cut(s) 1105
Alw26I GTCTC 2 cut(s) 262, 494
AoxI GGCC 1 cut(s) 612
ApeKI GCWGC 5 cut(s) 54, 101, 194, 629, 1105
ApoI RAATTY 2 cut(s) 1094, 1118
AspLEI GCGC 1 cut(s) 875
AspS9I GGNCC 1 cut(s) 613
AsuC2I CCSGG 2 cut(s) 505, 1020
AsuHPI GGTGA 3 cut(s) 92, 391, 865
BbsI GAAGAC 1 cut(s) 739
BbvI GCAGC 5 cut(s) 66, 88, 206, 641, 1092
BcnI CCSGG 2 cut(s) 505, 1020
BcoDI GTCTC 2 cut(s) 262, 494
BfaI CTAG 1 cut(s) 284
BfmI CTRYAG 1 cut(s) 12
BisI GCNGC 6 cut(s) 55, 102, 195, 630, 1106, 1109
BlsI GCNGC 6 cut(s) 56, 103, 196, 631, 1107, 1110
Bme1390I CCNGG 2 cut(s) 505, 1020
BmgT120I GGNCC 1 cut(s) 613
BmrFI CCNGG 2 cut(s) 505, 1020
BmrI ACTGGG 1 cut(s) 610
BmsI GCATC 6 cut(s) 382, 536, 616, 850, 873, 1004
BmuI ACTGGG 1 cut(s) 610
BpiI GAAGAC 1 cut(s) 739
BpuMI CCSGG 2 cut(s) 505, 1020
Bsa29I ATCGAT 1 cut(s) 723
BsaI GGTCTC 1 cut(s) 494
Bsc4I CCNNNNNNNGG 3 cut(s) 329, 557, 764
Bse1I ACTGG 2 cut(s) 402, 616
BseCI ATCGAT 1 cut(s) 723
BseGI GGATG 5 cut(s) 236, 259, 460, 671, 694
BseLI CCNNNNNNNGG 3 cut(s) 329, 557, 764
BseNI ACTGG 2 cut(s) 402, 616
BseRI GAGGAG 1 cut(s) 871
BseXI GCAGC 5 cut(s) 66, 88, 206, 641, 1092
BseYI CCCAGC 1 cut(s) 971
Bsh1236I CGCG 2 cut(s) 306, 591
BshFI GGCC 1 cut(s) 614
BshVI ATCGAT 1 cut(s) 723
BsiSI CCGG 2 cut(s) 504, 1020
BslI CCNNNNNNNGG 3 cut(s) 329, 557, 764
BsmAI GTCTC 2 cut(s) 262, 494
BsnI GGCC 1 cut(s) 614
Bso31I GGTCTC 1 cut(s) 494
Bsp1407I TGTACA 1 cut(s) 691
Bsp143I GATC 5 cut(s) 301, 363, 724, 736, 1140
BspACI CCGC 4 cut(s) 589, 924, 1060, 1109
BspANI GGCC 1 cut(s) 614
BspDI ATCGAT 1 cut(s) 723
BspFNI CGCG 2 cut(s) 306, 591
BspTNI GGTCTC 1 cut(s) 494
BsrGI TGTACA 1 cut(s) 691
BsrI ACTGG 2 cut(s) 402, 616
BssMI GATC 5 cut(s) 301, 363, 724, 736, 1140
Bst4CI ACNGT 3 cut(s) 72, 297, 732
Bst6I CTCTTC 2 cut(s) 285, 492
BstAUI TGTACA 1 cut(s) 691
BstC8I GCNNGC 2 cut(s) 331, 871
BstDEI CTNAG 1 cut(s) 567
BstF5I GGATG 5 cut(s) 236, 259, 460, 671, 694
BstFNI CGCG 2 cut(s) 306, 591
BstHHI GCGC 1 cut(s) 875
BstKTI GATC 5 cut(s) 304, 366, 727, 739, 1143
BstMAI GTCTC 2 cut(s) 262, 494
BstMBI GATC 5 cut(s) 301, 363, 724, 736, 1140
BstMWI GCNNNNNNNGC 2 cut(s) 110, 968
BstNSI RCATGY 2 cut(s) 411, 879
BstSCI CCNGG 2 cut(s) 503, 1018
BstSFI CTRYAG 1 cut(s) 12
BstUI CGCG 2 cut(s) 306, 591
BstV1I GCAGC 5 cut(s) 66, 88, 206, 641, 1092
BstV2I GAAGAC 1 cut(s) 739
Bsu15I ATCGAT 1 cut(s) 723
BsuRI GGCC 1 cut(s) 614
BsuTUI ATCGAT 1 cut(s) 723
BtsCI GGATG 5 cut(s) 236, 259, 460, 671, 694
BtsIMutI CAGTG 2 cut(s) 409, 943
Cac8I GCNNGC 2 cut(s) 331, 871
CfoI GCGC 1 cut(s) 875
Cfr13I GGNCC 1 cut(s) 613
ClaI ATCGAT 1 cut(s) 723
Csp6I GTAC 2 cut(s) 658, 692
CviJI RGCY 8 cut(s) 11, 113, 333, 360, 561, 614, 971, 1105
CviKI_1 RGCY 8 cut(s) 11, 113, 333, 360, 561, 614, 971, 1105
CviQI GTAC 2 cut(s) 658, 692
DdeI CTNAG 1 cut(s) 567
DpnI GATC 5 cut(s) 303, 365, 726, 738, 1142
DpnII GATC 5 cut(s) 301, 363, 724, 736, 1140
DraI TTTAAA 1 cut(s) 1029
Eam1104I CTCTTC 2 cut(s) 285, 492
EarI CTCTTC 2 cut(s) 285, 492
Eco31I GGTCTC 1 cut(s) 494
EcoT22I ATGCAT 1 cut(s) 48
FauI CCCGC 1 cut(s) 596
Fnu4HI GCNGC 6 cut(s) 55, 102, 195, 630, 1106, 1109
FokI GGATG 5 cut(s) 243, 266, 447, 678, 701
Fsp4HI GCNGC 6 cut(s) 55, 102, 195, 630, 1106, 1109
FspBI CTAG 1 cut(s) 284
GlaI GCGC 1 cut(s) 874
GluI GCNGC 6 cut(s) 55, 102, 195, 630, 1106, 1109
GsaI CCCAGC 1 cut(s) 975
HaeIII GGCC 1 cut(s) 614
HapII CCGG 2 cut(s) 504, 1020
HhaI GCGC 1 cut(s) 875
Hin6I GCGC 1 cut(s) 873
HinP1I GCGC 1 cut(s) 873
HinfI GANTC 7 cut(s) 158, 167, 393, 445, 593, 602, 861
HpaII CCGG 2 cut(s) 504, 1020
HphI GGTGA 3 cut(s) 92, 391, 865
Hpy166II GTNNAC 3 cut(s) 259, 534, 694
Hpy188I TCNGA 4 cut(s) 63, 354, 625, 741
Hpy188III TCNNGA 6 cut(s) 155, 377, 597, 845, 1005, 1115
Hpy8I GTNNAC 3 cut(s) 259, 534, 694
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 3 cut(s) 72, 297, 732
HpyCH4V TGCA 9 cut(s) 46, 194, 244, 373, 407, 629, 679, 841, 1056
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 968
HpyF3I CTNAG 1 cut(s) 567
HspAI GCGC 1 cut(s) 873
Kzo9I GATC 5 cut(s) 301, 363, 724, 736, 1140
Lsp1109I GCAGC 5 cut(s) 66, 88, 206, 641, 1092
LweI GCATC 6 cut(s) 382, 536, 616, 850, 873, 1004
MaeI CTAG 1 cut(s) 284
MaeIII GTNAC 2 cut(s) 442, 910
MalI GATC 5 cut(s) 303, 365, 726, 738, 1142
MboI GATC 5 cut(s) 301, 363, 724, 736, 1140
MluCI AATT 6 cut(s) 215, 488, 650, 956, 1094, 1118
MlyI GAGTC 4 cut(s) 152, 176, 439, 611
MmeI TCCRAC 1 cut(s) 1054
Mph1103I ATGCAT 1 cut(s) 48
MseI TTAA 5 cut(s) 288, 315, 383, 719, 1028
MspI CCGG 2 cut(s) 504, 1020
MspR9I CCNGG 2 cut(s) 505, 1020
MvnI CGCG 2 cut(s) 306, 591
MwoI GCNNNNNNNGC 2 cut(s) 110, 968
NciI CCSGG 2 cut(s) 505, 1020
NdeII GATC 5 cut(s) 301, 363, 724, 736, 1140
NmuCI GTSAC 2 cut(s) 442, 910
NsiI ATGCAT 1 cut(s) 48
NspI RCATGY 2 cut(s) 411, 879
PfeI GAWTC 3 cut(s) 393, 593, 861
PkrI GCNGC 6 cut(s) 56, 103, 196, 631, 1107, 1110
PleI GAGTC 4 cut(s) 152, 175, 439, 610
PpsI GAGTC 4 cut(s) 152, 175, 439, 610
PspFI CCCAGC 1 cut(s) 971
PspPI GGNCC 1 cut(s) 613
RsaI GTAC 2 cut(s) 659, 693
RsaNI GTAC 2 cut(s) 658, 692
SaqAI TTAA 5 cut(s) 288, 315, 383, 719, 1028
SatI GCNGC 6 cut(s) 55, 102, 195, 630, 1106, 1109
Sau3AI GATC 5 cut(s) 301, 363, 724, 736, 1140
Sau96I GGNCC 1 cut(s) 613
SchI GAGTC 4 cut(s) 152, 176, 439, 611
ScrFI CCNGG 2 cut(s) 505, 1020
SetI ASST 4 cut(s) 252, 687, 1091, 1107
SfaNI GCATC 6 cut(s) 382, 536, 616, 850, 873, 1004
SfcI CTRYAG 1 cut(s) 12
Sse9I AATT 6 cut(s) 215, 488, 650, 956, 1094, 1118
SsiI CCGC 4 cut(s) 589, 924, 1060, 1109
SspMI CTAG 1 cut(s) 284
StyD4I CCNGG 2 cut(s) 503, 1018
TaaI ACNGT 3 cut(s) 72, 297, 732
TaqI TCGA 1 cut(s) 723
TasI AATT 6 cut(s) 215, 488, 650, 956, 1094, 1118
TatI WGTACW 2 cut(s) 657, 691
TauI GCSGC 1 cut(s) 1111
TfiI GAWTC 3 cut(s) 393, 593, 861
Tru1I TTAA 5 cut(s) 288, 315, 383, 719, 1028
Tru9I TTAA 5 cut(s) 288, 315, 383, 719, 1028
TscAI CASTG 2 cut(s) 409, 943
TseFI GTSAC 2 cut(s) 442, 910
TseI GCWGC 5 cut(s) 54, 101, 194, 629, 1105
Tsp45I GTSAC 2 cut(s) 442, 910
TspDTI ATGAA 4 cut(s) 331, 428, 896, 1113
TspRI CASTG 2 cut(s) 409, 943
XapI RAATTY 2 cut(s) 1094, 1118
XceI RCATGY 2 cut(s) 411, 879
XcmI CCANNNNNNNNNTGG 3 cut(s) 471, 808, 939
XspI CTAG 1 cut(s) 284
Zsp2I ATGCAT 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.