FvH4_3g14860

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000364
Physical Location & Seq
Forward (+)
9177357 .. 9180009
2653 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g14860.t2

Sequence Viewer

Length: 750 bp
ATGGAAGTCTTTGGGTATGAGAAGCGTATCAAGCACTATACCAGCTCTCAGAAAATACTATTGGTCGGAGAAGGAGACTTCTCCTTTGCCGTTTCCTTAGCCAGAGCTTTCGGCTCTTCCATCAACATGGTCGCCACTTCTCTAGACACCAGAGAGGCATTAGTGGTGAAGTATTCAAGGGCTATGAGCAATGTGATGGAATTGAAGAAGAGGGGATGCGTAGTACTGCATGAAGTGGATGTGCATACAATGAGCAGACACCCTTTTCTGAGTGATATTCGGTTTGATCGAATAGTCTACAATTTTCCTCATGCCGGCTACTTGCATGGTCAAAAATCATCCGAGCATAACCAGTATCAAATTTGGTATCATCAGGATTTGGTGAGGGATTTCTTCAGAAATGCGTGTGAAATGCTTACTGAAATGGGAGAAATTCATGTCACACACAAGACAACATATCCTTTCAGTGAATGGGAAATAGTGAAGTTAGCAGGACAGGTTGGGTTGTATTTGCTACAGGAAGAGAAGTTCTCAAGATGGGATTATCCGGGCTATGAAAATAAGAGAGGAGCTGGGTTATGTGATCAAACTTTTCCTGTTGGAGAATGTAGCACCTTCAAGTTTTCCAAGCTGTTGTATCATTCTACACCTTACAGTTACCACCTTGACTTGATTCGGGCTTACGGTCAGGATTCTCGAATTAACGGTCCTCAGATACACCATGCACCACTGCCATTCTTTCATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

250

Amino Acids

28.96

Weight (kDa)

7.26

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 20 - 189 5.1e-54 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 297
AcsI RAATTY 2 cut(s) 360, 432
AcuI CTGAAG 1 cut(s) 379
AfaI GTAC 1 cut(s) 225
AfiI CCNNNNNNNGG 1 cut(s) 314
AgsI TTSAA 3 cut(s) 177, 205, 619
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AloI GAACNNNNNNTCC 2 cut(s) 512, 544
AluBI AGCT 4 cut(s) 45, 107, 572, 631
AluI AGCT 4 cut(s) 45, 107, 572, 631
Alw26I GTCTC 1 cut(s) 69
ApoI RAATTY 2 cut(s) 360, 432
ArsI GACNNNNNNTTYG 4 cut(s) 68, 100, 691, 723
AspS9I GGNCC 1 cut(s) 707
AsuC2I CCSGG 1 cut(s) 549
AsuHPI GGTGA 2 cut(s) 178, 394
AvaII GGWCC 1 cut(s) 707
BccI CCATC 3 cut(s) 128, 190, 531
BceAI ACGGC 1 cut(s) 74
BclI TGATCA 1 cut(s) 583
BcnI CCSGG 1 cut(s) 549
BcoDI GTCTC 1 cut(s) 69
BfaI CTAG 1 cut(s) 143
BfmI CTRYAG 1 cut(s) 515
BmcAI AGTACT 1 cut(s) 225
Bme1390I CCNGG 1 cut(s) 549
Bme18I GGWCC 1 cut(s) 707
BmgT120I GGNCC 1 cut(s) 707
BmrFI CCNGG 1 cut(s) 549
BmsI GCATC 1 cut(s) 206
BplI GAGNNNNNCTC 2 cut(s) 515, 547
Bpu10I CCTNAGC 1 cut(s) 97
BpuEI CTTGAG 1 cut(s) 517
BpuMI CCSGG 1 cut(s) 549
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 1 cut(s) 352
Bse3DI GCAATG 1 cut(s) 196
BseGI GGATG 3 cut(s) 221, 244, 338
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMI GCAATG 1 cut(s) 196
BseMII CTCAG 3 cut(s) 62, 260, 725
BseNI ACTGG 1 cut(s) 352
BseRI GAGGAG 1 cut(s) 582
BseYI CCCAGC 1 cut(s) 572
BsiSI CCGG 2 cut(s) 315, 548
BslI CCNNNNNNNGG 1 cut(s) 314
BsmAI GTCTC 1 cut(s) 69
Bsp143I GATC 2 cut(s) 286, 583
BspCNI CTCAG 3 cut(s) 61, 261, 724
BspQI GCTCTTC 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 196
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 1 cut(s) 352
BssAI RCCGGY 1 cut(s) 314
BssMI GATC 2 cut(s) 286, 583
Bst4CI ACNGT 3 cut(s) 656, 686, 707
Bst6I CTCTTC 3 cut(s) 121, 203, 516
BstC8I GCNNGC 1 cut(s) 316
BstDEI CTNAG 4 cut(s) 48, 97, 269, 711
BstF5I GGATG 3 cut(s) 221, 244, 338
BstKTI GATC 2 cut(s) 289, 586
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 2 cut(s) 286, 583
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstSCI CCNGG 1 cut(s) 547
BstSFI CTRYAG 1 cut(s) 515
BstXI CCANNNNNNTGG 1 cut(s) 127
BtsCI GGATG 3 cut(s) 221, 244, 338
BtsI GCAGTG 1 cut(s) 728
BtsIMutI CAGTG 2 cut(s) 472, 728
Cac8I GCNNGC 1 cut(s) 316
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 707
Csp6I GTAC 1 cut(s) 224
CviAII CATG 6 cut(s) 127, 230, 311, 326, 437, 722
CviQI GTAC 1 cut(s) 224
DdeI CTNAG 4 cut(s) 48, 97, 269, 711
DpnI GATC 2 cut(s) 288, 585
DpnII GATC 2 cut(s) 286, 583
Eam1104I CTCTTC 3 cut(s) 121, 203, 516
EarI CTCTTC 3 cut(s) 121, 203, 516
Eco47I GGWCC 1 cut(s) 707
Eco57I CTGAAG 1 cut(s) 379
FaeI CATG 6 cut(s) 130, 233, 314, 329, 440, 725
FatI CATG 6 cut(s) 126, 229, 310, 325, 436, 721
FauNDI CATATG 1 cut(s) 744
FbaI TGATCA 1 cut(s) 583
FblI GTMKAC 1 cut(s) 297
FokI GGATG 3 cut(s) 228, 251, 325
FspBI CTAG 1 cut(s) 143
GsaI CCCAGC 1 cut(s) 576
HapII CCGG 2 cut(s) 315, 548
Hin1II CATG 6 cut(s) 130, 233, 314, 329, 440, 725
HinfI GANTC 2 cut(s) 673, 692
HpaII CCGG 2 cut(s) 315, 548
HphI GGTGA 2 cut(s) 178, 394
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 6 cut(s) 51, 68, 270, 343, 398, 714
Hpy188III TCNNGA 5 cut(s) 143, 374, 534, 689, 696
Hpy8I GTNNAC 1 cut(s) 298
HpyAV CCTTC 2 cut(s) 65, 625
HpyCH4III ACNGT 3 cut(s) 656, 686, 707
HpyCH4V TGCA 4 cut(s) 229, 244, 325, 725
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 4 cut(s) 48, 97, 269, 711
Hsp92II CATG 6 cut(s) 130, 233, 314, 329, 440, 725
KroI GCCGGC 1 cut(s) 314
KroNI GCCGGC 1 cut(s) 316
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 2 cut(s) 286, 583
LguI GCTCTTC 1 cut(s) 121
LmnI GCTCC 1 cut(s) 569
LweI GCATC 1 cut(s) 206
MaeI CTAG 1 cut(s) 143
MaeIII GTNAC 2 cut(s) 439, 656
MalI GATC 2 cut(s) 288, 585
MboI GATC 2 cut(s) 286, 583
MboII GAAGA 5 cut(s) 108, 217, 220, 385, 533
MluCI AATT 5 cut(s) 200, 301, 360, 432, 699
MmeI TCCRAC 2 cut(s) 46, 580
MnlI CCTC 6 cut(s) 148, 204, 318, 378, 560, 720
MroNI GCCGGC 1 cut(s) 314
MseI TTAA 1 cut(s) 702
MslI CAYNNNNRTG 1 cut(s) 125
MspI CCGG 2 cut(s) 315, 548
MspR9I CCNGG 1 cut(s) 549
MwoI GCNNNNNNNGC 1 cut(s) 31
NaeI GCCGGC 1 cut(s) 316
NciI CCSGG 1 cut(s) 549
NdeI CATATG 1 cut(s) 744
NdeII GATC 2 cut(s) 286, 583
NgoMIV GCCGGC 1 cut(s) 314
NlaIII CATG 6 cut(s) 130, 233, 314, 329, 440, 725
NmuCI GTSAC 1 cut(s) 439
PciSI GCTCTTC 1 cut(s) 121
PcsI WCGNNNNNNNCGW 1 cut(s) 286
PdiI GCCGGC 1 cut(s) 316
PfeI GAWTC 2 cut(s) 673, 692
PspFI CCCAGC 1 cut(s) 572
PspPI GGNCC 1 cut(s) 707
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
RseI CAYNNNNRTG 1 cut(s) 125
SapI GCTCTTC 1 cut(s) 121
SaqAI TTAA 1 cut(s) 702
Sau3AI GATC 2 cut(s) 286, 583
Sau96I GGNCC 1 cut(s) 707
ScaI AGTACT 1 cut(s) 225
ScrFI CCNGG 1 cut(s) 549
SetI ASST 8 cut(s) 47, 109, 501, 574, 617, 633, 652, 666
SfaNI GCATC 1 cut(s) 206
SfcI CTRYAG 1 cut(s) 515
SinI GGWCC 1 cut(s) 707
SmiMI CAYNNNNRTG 1 cut(s) 125
SmlI CTYRAG 1 cut(s) 532
SmoI CTYRAG 1 cut(s) 532
Sse9I AATT 5 cut(s) 200, 301, 360, 432, 699
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 1 cut(s) 547
TaaI ACNGT 3 cut(s) 656, 686, 707
TaqI TCGA 2 cut(s) 289, 697
TasI AATT 5 cut(s) 200, 301, 360, 432, 699
TatI WGTACW 1 cut(s) 223
TfiI GAWTC 2 cut(s) 673, 692
Tru1I TTAA 1 cut(s) 702
Tru9I TTAA 1 cut(s) 702
TscAI CASTG 2 cut(s) 472, 735
TseFI GTSAC 1 cut(s) 439
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 4 cut(s) 246, 425, 570, 731
TspRI CASTG 2 cut(s) 472, 735
VpaK11BI GGWCC 1 cut(s) 707
XapI RAATTY 2 cut(s) 360, 432
XbaI TCTAGA 1 cut(s) 142
XmiI GTMKAC 1 cut(s) 297
XspI CTAG 1 cut(s) 143
ZrmI AGTACT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.