MD05G1233500.v1.1

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000364
Physical Location & Seq
Forward (+)
36619690 .. 36621387
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1233500.v1.1.491

Sequence Viewer

Length: 735 bp
ATGGAAGTGTTTAGCTTTGAGAGAAGGATTAAGAATTACAGCAACTATCAGAATATACTGTTGGTCGGAGAAGGCGACTTCTCTTTTGCCGTTTGCTTAGCCAGAGCCTTTGGCTTCGCTGCCAACATGGTTGCCACTTCTCGTGACACCAGAGAGTCGTTAATGGTGGAGTATTCAAAAGCTATGAGTAATGTGGAGGAGTTAGAGTCCAGGGAATGCACTGTATCGTATGAGGTGGATGTCCACTATATGAGCAGGCACCCTTTCCTAACCAACGTGCGCTTTGATCGCATAATCTATAATTTTCCTCACGCCGGTTACTTGCGTGGTTCCCGTTCCCGTGAAAGCGACATGTACCAGATTTGCCGGTGTTTAGTATCATCAGGATTTGGTCAGGGGATTCTTCTTGAATGCAAGGGAAATGCTGACGAGAGTAGGAGAAATTCATGTCACACACACAAGAAAAAATATCCCTTCAGTGAATGGAATATAGTGGATTTAGCTTATCAGGCTGGATTGATTTTGGTTCATGAAGAACCATTCTCGAAATGGGATTATCCAGGCTACGAAAATAAGAGAGGGGCTGGGATGTTTGACCAAACTTTTCCCGTTGGAATGTGTAGAACCTACAAATTTAAGATGTTGGACCGTTCTACCACTTCCGGTTTCCACCTTGGCGCTACTTCTTCGGGTCCATGGCCTAGATATTGTGGAATTTGGGGTCCTCACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

245

Amino Acids

28.13

Weight (kDa)

8.08

Isoelectric Point (pI)

56.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 20 - 193 7.5e-38 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 258
AcsI RAATTY 3 cut(s) 442, 632, 714
AcuI CTGAAG 1 cut(s) 460
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 314
AflIII ACRYGT 2 cut(s) 351, 729
AgsI TTSAA 2 cut(s) 177, 410
AjnI CCWGG 2 cut(s) 209, 559
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AluBI AGCT 3 cut(s) 15, 182, 503
AluI AGCT 3 cut(s) 15, 182, 503
AoxI GGCC 1 cut(s) 698
ApeKI GCWGC 1 cut(s) 119
ApoI RAATTY 3 cut(s) 442, 632, 714
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AspLEI GCGC 2 cut(s) 282, 680
AspS9I GGNCC 3 cut(s) 646, 692, 722
AvaII GGWCC 3 cut(s) 646, 692, 722
BanI GGYRCC 1 cut(s) 258
BauI CACGAG 1 cut(s) 141
BbvI GCAGC 1 cut(s) 106
BceAI ACGGC 1 cut(s) 74
BciT130I CCWGG 2 cut(s) 211, 561
BfaI CTAG 1 cut(s) 702
BfoI RGCGCY 1 cut(s) 681
BisI GCNGC 1 cut(s) 120
BlpI GCTNAGC 1 cut(s) 97
BlsI GCNGC 1 cut(s) 121
Bme1390I CCNGG 2 cut(s) 211, 561
Bme18I GGWCC 3 cut(s) 646, 692, 722
BmgT120I GGNCC 3 cut(s) 646, 692, 722
BmiI GGNNCC 4 cut(s) 260, 331, 693, 723
BmrFI CCNGG 2 cut(s) 211, 561
Bpu1102I GCTNAGC 1 cut(s) 97
BsaJI CCNNGG 3 cut(s) 210, 673, 695
BsaWI WCCGGW 1 cut(s) 662
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse118I RCCGGY 2 cut(s) 314, 366
BseBI CCWGG 2 cut(s) 211, 561
BseDI CCNNGG 3 cut(s) 210, 673, 695
BseGI GGATG 2 cut(s) 244, 594
BseLI CCNNNNNNNGG 1 cut(s) 314
BseRI GAGGAG 1 cut(s) 212
BseXI GCAGC 1 cut(s) 106
BseYI CCCAGC 1 cut(s) 584
BshFI GGCC 1 cut(s) 700
BshNI GGYRCC 1 cut(s) 258
BsiSI CCGG 3 cut(s) 315, 367, 663
BslI CCNNNNNNNGG 1 cut(s) 314
BsmI GAATGC 2 cut(s) 221, 416
BsnI GGCC 1 cut(s) 700
Bsp143I GATC 1 cut(s) 286
Bsp1720I GCTNAGC 1 cut(s) 97
Bsp19I CCATGG 1 cut(s) 695
BspANI GGCC 1 cut(s) 700
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 4 cut(s) 260, 331, 693, 723
BspT107I GGYRCC 1 cut(s) 258
BsrFI RCCGGY 2 cut(s) 314, 366
BssAI RCCGGY 2 cut(s) 314, 366
BssECI CCNNGG 3 cut(s) 210, 673, 695
BssMI GATC 1 cut(s) 286
BssSI CACGAG 1 cut(s) 141
BssT1I CCWWGG 2 cut(s) 673, 695
Bst2BI CACGAG 1 cut(s) 141
Bst2UI CCWGG 2 cut(s) 211, 561
Bst4CI ACNGT 3 cut(s) 60, 223, 650
BstC8I GCNNGC 1 cut(s) 257
BstDEI CTNAG 1 cut(s) 97
BstDSI CCRYGG 1 cut(s) 695
BstF5I GGATG 2 cut(s) 244, 594
BstH2I RGCGCY 1 cut(s) 681
BstHHI GCGC 2 cut(s) 282, 680
BstKTI GATC 1 cut(s) 289
BstMBI GATC 1 cut(s) 286
BstMWI GCNNNNNNNGC 2 cut(s) 288, 509
BstNI CCWGG 2 cut(s) 211, 561
BstNSI RCATGY 2 cut(s) 355, 733
BstSCI CCNGG 2 cut(s) 209, 559
BstV1I GCAGC 1 cut(s) 106
BsuRI GGCC 1 cut(s) 700
BtgI CCRYGG 1 cut(s) 695
BtsCI GGATG 2 cut(s) 244, 594
BtsIMutI CAGTG 2 cut(s) 219, 484
Cac8I GCNNGC 1 cut(s) 257
CciI TCATGA 1 cut(s) 529
CfoI GCGC 2 cut(s) 282, 680
Cfr10I RCCGGY 2 cut(s) 314, 366
Cfr13I GGNCC 3 cut(s) 646, 692, 722
Csp6I GTAC 1 cut(s) 355
CviAII CATG 6 cut(s) 127, 352, 447, 530, 696, 730
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 1 cut(s) 97
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 673, 695
Eco47I GGWCC 3 cut(s) 646, 692, 722
Eco57I CTGAAG 1 cut(s) 460
EcoO109I RGGNCCY 1 cut(s) 722
EcoRII CCWGG 2 cut(s) 209, 559
EcoT14I CCWWGG 2 cut(s) 673, 695
ErhI CCWWGG 2 cut(s) 673, 695
FaeI CATG 6 cut(s) 130, 355, 450, 533, 699, 733
FatI CATG 6 cut(s) 126, 351, 446, 529, 695, 729
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 2 cut(s) 251, 601
Fsp4HI GCNGC 1 cut(s) 120
FspBI CTAG 1 cut(s) 702
GlaI GCGC 2 cut(s) 281, 679
GluI GCNGC 1 cut(s) 120
GsaI CCCAGC 1 cut(s) 588
HaeII RGCGCY 1 cut(s) 681
HaeIII GGCC 1 cut(s) 700
HapII CCGG 3 cut(s) 315, 367, 663
HhaI GCGC 2 cut(s) 282, 680
Hin1II CATG 6 cut(s) 130, 355, 450, 533, 699, 733
Hin6I GCGC 2 cut(s) 280, 678
HinP1I GCGC 2 cut(s) 280, 678
HinfI GANTC 3 cut(s) 155, 206, 400
HpaII CCGG 3 cut(s) 315, 367, 663
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 2 cut(s) 51, 68
Hpy188III TCNNGA 5 cut(s) 143, 384, 407, 530, 544
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 3 cut(s) 18, 65, 484
HpyCH4III ACNGT 3 cut(s) 60, 223, 650
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 2 cut(s) 219, 414
HpyF10VI GCNNNNNNNGC 2 cut(s) 288, 509
HpyF3I CTNAG 1 cut(s) 97
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 6 cut(s) 130, 355, 450, 533, 699, 733
HspAI GCGC 2 cut(s) 280, 678
Kzo9I GATC 1 cut(s) 286
Lsp1109I GCAGC 1 cut(s) 106
MaeI CTAG 1 cut(s) 702
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 3 cut(s) 143, 317, 449
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 3 cut(s) 395, 545, 678
MluCI AATT 5 cut(s) 34, 301, 442, 632, 714
MlyI GAGTC 2 cut(s) 164, 215
MmeI TCCRAC 3 cut(s) 46, 592, 624
MnlI CCTC 5 cut(s) 190, 226, 318, 572, 735
MseI TTAA 3 cut(s) 30, 161, 636
MspI CCGG 3 cut(s) 315, 367, 663
MspR9I CCNGG 2 cut(s) 211, 561
Mva1269I GAATGC 2 cut(s) 221, 416
MvaI CCWGG 2 cut(s) 211, 561
MwoI GCNNNNNNNGC 2 cut(s) 288, 509
NcoI CCATGG 1 cut(s) 695
NdeII GATC 1 cut(s) 286
NlaIII CATG 6 cut(s) 130, 355, 450, 533, 699, 733
NlaIV GGNNCC 4 cut(s) 260, 331, 693, 723
NmuCI GTSAC 2 cut(s) 143, 449
NspI RCATGY 2 cut(s) 355, 733
PagI TCATGA 1 cut(s) 529
PciI ACATGT 2 cut(s) 351, 729
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PctI GAATGC 2 cut(s) 221, 416
PfeI GAWTC 1 cut(s) 400
PkrI GCNGC 1 cut(s) 121
PleI GAGTC 2 cut(s) 163, 214
PpsI GAGTC 2 cut(s) 163, 214
PpuMI RGGWCCY 1 cut(s) 722
PscI ACATGT 2 cut(s) 351, 729
Psp5II RGGWCCY 1 cut(s) 722
Psp6I CCWGG 2 cut(s) 209, 559
PspFI CCCAGC 1 cut(s) 584
PspGI CCWGG 2 cut(s) 209, 559
PspN4I GGNNCC 4 cut(s) 260, 331, 693, 723
PspPI GGNCC 3 cut(s) 646, 692, 722
PspPPI RGGWCCY 1 cut(s) 722
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
SaqAI TTAA 3 cut(s) 30, 161, 636
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 1 cut(s) 286
Sau96I GGNCC 3 cut(s) 646, 692, 722
SchI GAGTC 2 cut(s) 164, 215
ScrFI CCNGG 2 cut(s) 211, 561
SetI ASST 7 cut(s) 17, 184, 237, 279, 505, 629, 675
SinI GGWCC 3 cut(s) 646, 692, 722
Sse9I AATT 5 cut(s) 34, 301, 442, 632, 714
SspMI CTAG 1 cut(s) 702
StyD4I CCNGG 2 cut(s) 209, 559
StyI CCWWGG 2 cut(s) 673, 695
TaaI ACNGT 3 cut(s) 60, 223, 650
TaiI ACGT 1 cut(s) 279
TaqI TCGA 1 cut(s) 545
TasI AATT 5 cut(s) 34, 301, 442, 632, 714
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 3 cut(s) 30, 161, 636
Tru9I TTAA 3 cut(s) 30, 161, 636
TscAI CASTG 2 cut(s) 226, 484
TseFI GTSAC 2 cut(s) 143, 449
TseI GCWGC 1 cut(s) 119
Tsp45I GTSAC 2 cut(s) 143, 449
TspDTI ATGAA 3 cut(s) 435, 518, 546
TspRI CASTG 2 cut(s) 226, 484
VpaK11BI GGWCC 3 cut(s) 646, 692, 722
XapI RAATTY 3 cut(s) 442, 632, 714
XceI RCATGY 2 cut(s) 355, 733
XcmI CCANNNNNNNNNTGG 1 cut(s) 546
XspI CTAG 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.