MD10G1210300.v1.1

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000364
Physical Location & Seq
Forward (+)
30978351 .. 30980572
2222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1210300.v1.1.491

Sequence Viewer

Length: 348 bp
ATGAGATTGCATATGGGTGGCCGACAATACCATCAAGTTGGGCCCCACTTCCCCTCAAAGCTTCATCAGAATTTGGTTACAAATTACTTCAAGAGTGCGCATAAAATGCTGACCAACTGTGGAGAGATTCATGTGACAAACAAGACTGCACATCCTTTCAAAGAGTGGAAGATTGTAGAGTTGGCAGAAGAATTTGGGCTGCATTTGGTTGAGGAATCAGAGTTATCACTATATGATTATCCAGGTTATTTAAACAGGAGAGGAAGTGGGATTGCTCTATGGAATTTCCTGTGGGAAAGTGTAGTACCTTCAAAGTTTGCCAAGCAAGCTGCAAATAATGAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

116

Amino Acids

13.29

Weight (kDa)

7.94

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 18 - 87 2.3e-15 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 99
AcoI YGGCCR 1 cut(s) 19
AcsI RAATTY 3 cut(s) 70, 191, 283
AfaI GTAC 1 cut(s) 306
AgsI TTSAA 3 cut(s) 91, 160, 312
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 2 cut(s) 61, 329
AluI AGCT 2 cut(s) 61, 329
AoxI GGCC 2 cut(s) 19, 41
ApaI GGGCCC 1 cut(s) 45
ApeKI GCWGC 2 cut(s) 199, 329
ApoI RAATTY 3 cut(s) 70, 191, 283
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 2 cut(s) 41, 42
BaeGI GKGCMC 1 cut(s) 45
BanII GRGCYC 1 cut(s) 45
BbvI GCAGC 2 cut(s) 186, 316
BccI CCATC 1 cut(s) 39
BciT130I CCWGG 1 cut(s) 243
BisI GCNGC 2 cut(s) 200, 330
BlsI GCNGC 2 cut(s) 201, 331
Bme1390I CCNGG 1 cut(s) 243
BmgT120I GGNCC 2 cut(s) 41, 42
BmiI GGNNCC 2 cut(s) 43, 44
BmrFI CCNGG 1 cut(s) 243
BsaXI ACNNNNNCTCC 2 cut(s) 250, 280
BseBI CCWGG 1 cut(s) 243
BseGI GGATG 1 cut(s) 151
BseSI GKGCMC 1 cut(s) 45
BseXI GCAGC 2 cut(s) 186, 316
BsgI GTGCAG 1 cut(s) 132
BshFI GGCC 2 cut(s) 21, 43
BsnI GGCC 2 cut(s) 21, 43
Bsp120I GGGCCC 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 45
BspANI GGCC 2 cut(s) 21, 43
BspLI GGNNCC 2 cut(s) 43, 44
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 119
BstAPI GCANNNNNTGC 1 cut(s) 106
BstC8I GCNNGC 1 cut(s) 327
BstF5I GGATG 1 cut(s) 151
BstHHI GCGC 1 cut(s) 100
BstMWI GCNNNNNNNGC 2 cut(s) 106, 326
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 1 cut(s) 241
BstSLI GKGCMC 1 cut(s) 45
BstV1I GCAGC 2 cut(s) 186, 316
BstXI CCANNNNNNTGG 1 cut(s) 38
BsuRI GGCC 2 cut(s) 21, 43
BtsCI GGATG 1 cut(s) 151
Cac8I GCNNGC 1 cut(s) 327
CfoI GCGC 1 cut(s) 100
Cfr13I GGNCC 2 cut(s) 41, 42
Csp6I GTAC 1 cut(s) 305
CviAII CATG 1 cut(s) 131
CviJI RGCY 5 cut(s) 21, 43, 61, 199, 329
CviKI_1 RGCY 5 cut(s) 21, 43, 61, 199, 329
CviQI GTAC 1 cut(s) 305
DraI TTTAAA 1 cut(s) 252
EaeI YGGCCR 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 45
EcoO109I RGGNCCY 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 45
FaeI CATG 1 cut(s) 134
FaiI YATR 7 cut(s) 12, 14, 102, 132, 232, 234, 280
FatI CATG 1 cut(s) 130
FauNDI CATATG 1 cut(s) 12
Fnu4HI GCNGC 2 cut(s) 200, 330
FokI GGATG 1 cut(s) 138
FriOI GRGCYC 1 cut(s) 45
Fsp4HI GCNGC 2 cut(s) 200, 330
FspAI RTGCGCAY 1 cut(s) 99
FspI TGCGCA 1 cut(s) 99
GlaI GCGC 1 cut(s) 99
GluI GCNGC 2 cut(s) 200, 330
HaeIII GGCC 2 cut(s) 21, 43
HhaI GCGC 1 cut(s) 100
Hin1II CATG 1 cut(s) 134
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HindIII AAGCTT 1 cut(s) 59
HinfI GANTC 2 cut(s) 127, 215
Hpy188I TCNGA 2 cut(s) 69, 220
Hpy188III TCNNGA 1 cut(s) 91
HpyAV CCTTC 1 cut(s) 318
HpyCH4III ACNGT 1 cut(s) 119
HpyCH4V TGCA 4 cut(s) 10, 149, 202, 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 106, 326
Hsp92II CATG 1 cut(s) 134
HspAI GCGC 1 cut(s) 98
LpnPI CCDG 4 cut(s) 228, 241, 255, 302
Lsp1109I GCAGC 2 cut(s) 186, 316
MaeIII GTNAC 2 cut(s) 76, 133
MboII GAAGA 2 cut(s) 181, 200
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 4 cut(s) 70, 82, 191, 283
MnlI CCTC 3 cut(s) 64, 205, 254
MseI TTAA 1 cut(s) 251
MslI CAYNNNNRTG 1 cut(s) 15
MspR9I CCNGG 1 cut(s) 243
MvaI CCWGG 1 cut(s) 243
MwoI GCNNNNNNNGC 2 cut(s) 106, 326
NdeI CATATG 1 cut(s) 12
NlaIII CATG 1 cut(s) 134
NlaIV GGNNCC 2 cut(s) 43, 44
NmuCI GTSAC 1 cut(s) 133
NsbI TGCGCA 1 cut(s) 99
PfeI GAWTC 2 cut(s) 127, 215
PkrI GCNGC 2 cut(s) 201, 331
Psp6I CCWGG 1 cut(s) 241
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 2 cut(s) 43, 44
PspOMI GGGCCC 1 cut(s) 41
PspPI GGNCC 2 cut(s) 41, 42
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
RseI CAYNNNNRTG 1 cut(s) 15
SaqAI TTAA 1 cut(s) 251
SatI GCNGC 2 cut(s) 200, 330
Sau96I GGNCC 2 cut(s) 41, 42
ScrFI CCNGG 1 cut(s) 243
SduI GDGCHC 1 cut(s) 45
SetI ASST 5 cut(s) 63, 247, 310, 331, 346
SmiMI CAYNNNNRTG 1 cut(s) 15
Sse9I AATT 4 cut(s) 70, 82, 191, 283
StyD4I CCNGG 1 cut(s) 241
TaaI ACNGT 1 cut(s) 119
TasI AATT 4 cut(s) 70, 82, 191, 283
TfiI GAWTC 2 cut(s) 127, 215
Tru1I TTAA 1 cut(s) 251
Tru9I TTAA 1 cut(s) 251
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 2 cut(s) 199, 329
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 2 cut(s) 53, 119
XapI RAATTY 3 cut(s) 70, 191, 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.