MD05G1233600.v1.1

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000364
Physical Location & Seq
Forward (+)
36631561 .. 36632991
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1233600.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGCAGAGAAGAAGATTATGCACTACAGCAGCTCTCAGAAGATACTGTTAGTAGGTGAGGGCAACTTCTCTTTTGCTGCTTCCTTAGCCACTGCATTTGGCTCTGCAAACAACATTGTCGCCACTTCTCTCGACTCCAAAGAGTCATTTATGGACAAGTACCCAGATGCAGTGAGGAAATGGAAGAAGTTAAAGGGAAAGGGATGTGTAGTATTGCATCAAGTGGATGTGGATACCATGAGCCAACACCCTCTGCTAGCTGCTAAACTATTTGATCGAGTCGTCTTTAATTTCCCTCACGCGGGCTTCTTCGGCATGGAAAGCCAGAGGTTTCAAATTGAGTTGCATCAAGATCTGGTCAAGCGGTTCTTCGCCGCTGCGTCTGAAATGCTGACGGGAAGAGGAGAAGTTCATGTGACACACAAGACCGCTTATCCTTTCAGCAACTGGAACATAGTGAAGTTAGCAGAAGAGGTAGGGTTATATCTGGTTGAGGAAGCACCTTTCACGCGCGCGGATTATCCAGGTTATCTCAATAAGAGAGGAAGTGGGAGAAAATGCAACCGGACATTTCGTGTTGGACAGTGTAGTACCTACAAATTTGCCAAGCTACCACTTCAACTTCTGGTTCTTGATTCACCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

216

Amino Acids

24.23

Weight (kDa)

9.36

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 16 - 181 2.3e-54 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 302, 511, 513, 515
AciI CCGC 5 cut(s) 302, 364, 375, 429, 515
AcsI RAATTY 1 cut(s) 599
AfaI GTAC 2 cut(s) 161, 592
AfiI CCNNNNNNNGG 2 cut(s) 301, 302
AgsI TTSAA 2 cut(s) 335, 620
AjnI CCWGG 1 cut(s) 523
AluBI AGCT 3 cut(s) 33, 260, 610
AluI AGCT 3 cut(s) 33, 260, 610
AlwNI CAGNNNCTG 1 cut(s) 447
ApeKI GCWGC 4 cut(s) 30, 77, 260, 377
ApoI RAATTY 1 cut(s) 599
AspLEI GCGC 2 cut(s) 513, 515
AsuHPI GGTGA 2 cut(s) 68, 630
AsuNHI GCTAGC 1 cut(s) 256
BbvI GCAGC 4 cut(s) 42, 64, 247, 364
BciT130I CCWGG 1 cut(s) 525
BciVI GTATCC 1 cut(s) 226
BfaI CTAG 1 cut(s) 257
BfmI CTRYAG 1 cut(s) 25
BfuI GTATCC 1 cut(s) 226
BglII AGATCT 1 cut(s) 352
BisI GCNGC 5 cut(s) 31, 78, 261, 375, 378
BlsI GCNGC 5 cut(s) 32, 79, 262, 376, 379
Bme1390I CCNGG 1 cut(s) 525
BmrFI CCNGG 1 cut(s) 525
BmsI GCATC 3 cut(s) 157, 226, 355
BmtI GCTAGC 1 cut(s) 260
Bpu10I CCTNAGC 1 cut(s) 85
BsaWI WCCGGW 1 cut(s) 564
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 302
Bse1I ACTGG 1 cut(s) 452
BseBI CCWGG 1 cut(s) 525
BseGI GGATG 2 cut(s) 209, 232
BseLI CCNNNNNNNGG 2 cut(s) 301, 302
BseMII CTCAG 1 cut(s) 50
BseNI ACTGG 1 cut(s) 452
BsePI GCGCGC 1 cut(s) 511
BseRI GAGGAG 1 cut(s) 417
BseXI GCAGC 4 cut(s) 42, 64, 247, 364
Bsh1236I CGCG 4 cut(s) 302, 511, 513, 515
BsiSI CCGG 1 cut(s) 565
BslI CCNNNNNNNGG 2 cut(s) 301, 302
Bsp143I GATC 2 cut(s) 274, 352
BspACI CCGC 5 cut(s) 302, 364, 375, 429, 515
BspCNI CTCAG 1 cut(s) 49
BspFNI CGCG 4 cut(s) 302, 511, 513, 515
BspOI GCTAGC 1 cut(s) 260
BsrI ACTGG 1 cut(s) 452
BssHII GCGCGC 1 cut(s) 511
BssMI GATC 2 cut(s) 274, 352
Bst2UI CCWGG 1 cut(s) 525
Bst4CI ACNGT 2 cut(s) 48, 585
Bst6I CTCTTC 2 cut(s) 394, 465
BstC8I GCNNGC 3 cut(s) 258, 304, 513
BstDEI CTNAG 2 cut(s) 36, 85
BstF5I GGATG 2 cut(s) 209, 232
BstFNI CGCG 4 cut(s) 302, 511, 513, 515
BstHHI GCGC 2 cut(s) 513, 515
BstKTI GATC 2 cut(s) 277, 355
BstMBI GATC 2 cut(s) 274, 352
BstMWI GCNNNNNNNGC 3 cut(s) 86, 312, 321
BstNI CCWGG 1 cut(s) 525
BstSCI CCNGG 1 cut(s) 523
BstSFI CTRYAG 1 cut(s) 25
BstUI CGCG 4 cut(s) 302, 511, 513, 515
BstV1I GCAGC 4 cut(s) 42, 64, 247, 364
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BsuI GTATCC 1 cut(s) 226
BtsCI GGATG 2 cut(s) 209, 232
BtsI GCAGTG 2 cut(s) 90, 177
BtsIMutI CAGTG 3 cut(s) 90, 177, 590
Cac8I GCNNGC 3 cut(s) 258, 304, 513
CaiI CAGNNNCTG 1 cut(s) 447
CfoI GCGC 2 cut(s) 513, 515
CseI GACGC 1 cut(s) 369
Csp6I GTAC 2 cut(s) 160, 591
CspCI CAANNNNNGTGG 2 cut(s) 79, 114
CviAII CATG 3 cut(s) 238, 316, 413
CviJI RGCY 8 cut(s) 33, 89, 102, 243, 260, 306, 324, 610
CviKI_1 RGCY 8 cut(s) 33, 89, 102, 243, 260, 306, 324, 610
CviQI GTAC 2 cut(s) 160, 591
DdeI CTNAG 2 cut(s) 36, 85
DpnI GATC 2 cut(s) 276, 354
DpnII GATC 2 cut(s) 274, 352
Eam1104I CTCTTC 2 cut(s) 394, 465
EarI CTCTTC 2 cut(s) 394, 465
EcoRII CCWGG 1 cut(s) 523
FaeI CATG 3 cut(s) 241, 319, 416
FaiI YATR 7 cut(s) 20, 152, 239, 317, 414, 455, 484
FalI AAGNNNNNCTT 2 cut(s) 353, 385
FatI CATG 3 cut(s) 237, 315, 412
FauI CCCGC 1 cut(s) 295
Fnu4HI GCNGC 5 cut(s) 31, 78, 261, 375, 378
FokI GGATG 2 cut(s) 216, 239
Fsp4HI GCNGC 5 cut(s) 31, 78, 261, 375, 378
FspBI CTAG 1 cut(s) 257
GlaI GCGC 2 cut(s) 512, 514
GluI GCNGC 5 cut(s) 31, 78, 261, 375, 378
HapII CCGG 1 cut(s) 565
HgaI GACGC 1 cut(s) 369
HhaI GCGC 2 cut(s) 513, 515
Hin1II CATG 3 cut(s) 241, 319, 416
Hin6I GCGC 2 cut(s) 511, 513
HinP1I GCGC 2 cut(s) 511, 513
HinfI GANTC 4 cut(s) 134, 143, 279, 635
HpaII CCGG 1 cut(s) 565
HphI GGTGA 2 cut(s) 68, 630
Hpy188I TCNGA 2 cut(s) 39, 385
Hpy188III TCNNGA 3 cut(s) 131, 350, 632
HpyCH4III ACNGT 2 cut(s) 48, 585
HpyCH4V TGCA 7 cut(s) 22, 95, 107, 170, 217, 346, 561
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 312, 321
HpyF3I CTNAG 2 cut(s) 36, 85
Hsp92II CATG 3 cut(s) 241, 319, 416
HspAI GCGC 2 cut(s) 511, 513
Kzo9I GATC 2 cut(s) 274, 352
LpnPI CCDG 9 cut(s) 177, 338, 341, 433, 473, 510, 537, 578, 611
Lsp1109I GCAGC 4 cut(s) 42, 64, 247, 364
LweI GCATC 3 cut(s) 157, 226, 355
MaeI CTAG 1 cut(s) 257
MaeIII GTNAC 1 cut(s) 415
MalI GATC 2 cut(s) 276, 354
MauBI CGCGCGCG 1 cut(s) 511
MboI GATC 2 cut(s) 274, 352
MboII GAAGA 8 cut(s) 22, 25, 52, 196, 301, 361, 411, 482
MflI RGATCY 1 cut(s) 352
MluCI AATT 3 cut(s) 289, 336, 599
MlyI GAGTC 3 cut(s) 128, 152, 288
MmeI TCCRAC 1 cut(s) 559
MnlI CCTC 9 cut(s) 52, 168, 261, 306, 321, 395, 466, 487, 536
MseI TTAA 2 cut(s) 191, 288
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 1 cut(s) 565
MspR9I CCNGG 1 cut(s) 525
MvaI CCWGG 1 cut(s) 525
MvnI CGCG 4 cut(s) 302, 511, 513, 515
MwoI GCNNNNNNNGC 3 cut(s) 86, 312, 321
NdeII GATC 2 cut(s) 274, 352
NheI GCTAGC 1 cut(s) 256
NlaIII CATG 3 cut(s) 241, 319, 416
NmuCI GTSAC 1 cut(s) 415
PauI GCGCGC 1 cut(s) 511
PfeI GAWTC 1 cut(s) 635
PkrI GCNGC 5 cut(s) 32, 79, 262, 376, 379
PleI GAGTC 3 cut(s) 128, 151, 287
PpsI GAGTC 3 cut(s) 128, 151, 287
Psp6I CCWGG 1 cut(s) 523
PspGI CCWGG 1 cut(s) 523
PstNI CAGNNNCTG 1 cut(s) 447
PsuI RGATCY 1 cut(s) 352
PteI GCGCGC 1 cut(s) 511
RsaI GTAC 2 cut(s) 161, 592
RsaNI GTAC 2 cut(s) 160, 591
SaqAI TTAA 2 cut(s) 191, 288
SatI GCNGC 5 cut(s) 31, 78, 261, 375, 378
Sau3AI GATC 2 cut(s) 274, 352
SchI GAGTC 3 cut(s) 128, 152, 288
ScrFI CCNGG 1 cut(s) 525
SfaNI GCATC 3 cut(s) 157, 226, 355
SfcI CTRYAG 1 cut(s) 25
Sse9I AATT 3 cut(s) 289, 336, 599
SsiI CCGC 5 cut(s) 302, 364, 375, 429, 515
SspMI CTAG 1 cut(s) 257
StyD4I CCNGG 1 cut(s) 523
TaaI ACNGT 2 cut(s) 48, 585
TaqI TCGA 2 cut(s) 132, 277
TasI AATT 3 cut(s) 289, 336, 599
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 1 cut(s) 635
Tru1I TTAA 2 cut(s) 191, 288
Tru9I TTAA 2 cut(s) 191, 288
TscAI CASTG 3 cut(s) 97, 177, 590
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 4 cut(s) 30, 77, 260, 377
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 1 cut(s) 401
TspRI CASTG 3 cut(s) 97, 177, 590
XapI RAATTY 1 cut(s) 599
XspI CTAG 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.