Rh5AG174100

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000364
Physical Location & Seq
Forward (+)
19226504 .. 19242534
16031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG174100.1

Sequence Viewer

Length: 1344 bp
ATGGAAGTCTTTGGGTTTGAGAAGCGTATCAAGCACTACACCAACTATCAGAGAATACTCTTGGTCGGAGAAGGAGACTTCTCCTTTGCCGTTTCCTTAGCCAGAGCTTTCGGCTCTTCCACCGAGATGGTCGCTACTTCTCTCGACACCAGAGAGTCATTGAGGGAGAAGTATTCAAAGGCTATGAGCAATGTGATGGAATTGGAGAACAGGGGATGCAGAGTACTTCATGAAGTGGATGTGCACAGAATGAGCCAACACCCTTTTCTGAGTTGTTTTCGGTTTGATCGAATAATCTACAATTTTCCTCATGCCGGTTACTTGCACGGTCAATCATCATCCGAGCACAACCAGTTTCAAATTTGGTTCCATCAGGATTTGGTGAGGGGATTCTTCAAGAATGCGCGTGAAATGCTTACCGAAATAGGAGAAATTCATGTGACACACAAGACAACATATCCTTTCAGTCGATGGGAAATAGTGAAGTTAGCAGAAGAGGTTGGGTTGTATTTGCTTCAGGAAGAAAAATTCTCAAAATGGGATTATCCAGGTTATGAAAATAAGAGAGGAGCTGGGTTATGTGATCAAACTTTTCCTGTTGGAGAATGTAGCACCTTCAAATTTGCCAAGCTATTGTACCATTCTACCCCTTCCAGTTACTGCATTGACTTGGTTCGGGCGTACGGTCAGGATTCTCGAATTAACGGTCCTTACATGCACCACTGTTTAATACACCCAATGGGCAAATTGACCAAGCAATTCCTTCCAAATGAAAAACTTAACTCTAGCTCTTTGATGACTATGGATAGATATGCAGCTGCAGAAAGCTTCTATAAGCTTGCTATGGCATTTGCCCCTGTCCCAGATCTTCATATAATGTGGTTACTTCATTTATGTGATGCACAACAAGATATGCAGTCGTGGGCAGAATCTGCTCAGTGTGCTGTCGCTGTGGCTGGTATTGTAATGCAGGCTTTGATGGCCAGAAATGATGGTGTTTGGAGCAAAGATCACATAACTGCTCTGCGTAAAATTTGTCCAATGGTCAGTAGTGAGATCAGCTCTGAGGCTGCTGCAGCTGAGGTAGAGGGATATGGTGCATCAAAACTTACTGTTGATTCTGCCGTGAAGTACCTACAACTCGCAAATAAGCTCTTTTCTCAACCTGAACTTTTTCATTTCTGTGCAAGCATTCTGGAACTTGTTATTCCAGTTTATAAAAGCAGGCGGGCATATGGACAGCTGAGCAAATGTCACACAATGCTTACTAATATATATGAATCAATCCTTGAGCAGGAATCAAGCCCAATTCCATTCACTGACGCAACATATGCTATAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

447

Amino Acids

50.95

Weight (kDa)

6.44

Isoelectric Point (pI)

50.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 20 - 189 1.4e-55 rRNA (uridine-N3-)-methyltransferase BTM5-like
DHR-2_Lobe_A PF06920 278 - 431 4.6e-34 DHR-2, Lobe A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1218
AccII CGCG 1 cut(s) 406
AciI CCGC 1 cut(s) 1228
AcoI YGGCCR 1 cut(s) 981
AcsI RAATTY 5 cut(s) 360, 432, 527, 620, 1032
AcuI CTGAAG 1 cut(s) 500
AfaI GTAC 4 cut(s) 225, 638, 683, 1133
AfiI CCNNNNNNNGG 2 cut(s) 314, 1294
AgsI TTSAA 4 cut(s) 177, 359, 397, 619
AjnI CCWGG 1 cut(s) 547
AjuI GAANNNNNNNTTGG 2 cut(s) 249, 281
Alw21I GWGCWC 2 cut(s) 246, 348
Alw26I GTCTC 1 cut(s) 69
Alw44I GTGCAC 1 cut(s) 242
AlwNI CAGNNNCTG 2 cut(s) 660, 932
AoxI GGCC 1 cut(s) 981
ApaLI GTGCAC 1 cut(s) 242
ApeKI GCWGC 5 cut(s) 815, 818, 1070, 1073, 1076
ApoI RAATTY 5 cut(s) 360, 432, 527, 620, 1032
ArsI GACNNNNNNTTYG 4 cut(s) 68, 100, 691, 723
Asp700I GAANNNNTTC 1 cut(s) 1173
AspLEI GCGC 1 cut(s) 406
AspS9I GGNCC 1 cut(s) 707
AsuHPI GGTGA 1 cut(s) 394
AvaII GGWCC 1 cut(s) 707
BaeGI GKGCMC 1 cut(s) 246
BalI TGGCCA 1 cut(s) 983
Bbv12I GWGCWC 2 cut(s) 246, 348
BbvCI CCTCAGC 1 cut(s) 1080
BbvI GCAGC 5 cut(s) 805, 827, 1057, 1060, 1088
BccI CCATC 6 cut(s) 121, 190, 378, 465, 973, 986
BceAI ACGGC 2 cut(s) 74, 1109
BciT130I CCWGG 1 cut(s) 549
BclI TGATCA 1 cut(s) 583
BcoDI GTCTC 1 cut(s) 69
BfaI CTAG 1 cut(s) 786
BfmI CTRYAG 3 cut(s) 819, 1074, 1335
BglII AGATCT 1 cut(s) 865
BisI GCNGC 5 cut(s) 816, 819, 1071, 1074, 1077
BlpI GCTNAGC 1 cut(s) 1244
BlsI GCNGC 5 cut(s) 817, 820, 1072, 1075, 1078
BmcAI AGTACT 1 cut(s) 225
Bme1390I CCNGG 1 cut(s) 549
Bme18I GGWCC 1 cut(s) 707
BmgT120I GGNCC 1 cut(s) 707
BmiI GGNNCC 1 cut(s) 368
BmrFI CCNGG 1 cut(s) 549
BmsI GCATC 3 cut(s) 206, 889, 1109
BplI GAGNNNNNCTC 2 cut(s) 1046, 1078
Bpu10I CCTNAGC 2 cut(s) 97, 1080
Bpu1102I GCTNAGC 1 cut(s) 1244
BpuEI CTTGAG 1 cut(s) 1310
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 1294
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 3 cut(s) 352, 654, 1211
Bse3DI GCAATG 1 cut(s) 196
BseBI CCWGG 1 cut(s) 549
BseGI GGATG 3 cut(s) 221, 244, 338
BseLI CCNNNNNNNGG 2 cut(s) 314, 1294
BseMI GCAATG 1 cut(s) 196
BseMII CTCAG 5 cut(s) 260, 950, 1056, 1071, 1235
BseNI ACTGG 3 cut(s) 352, 654, 1211
BseRI GAGGAG 1 cut(s) 582
BseSI GKGCMC 1 cut(s) 246
BseXI GCAGC 5 cut(s) 805, 827, 1057, 1060, 1088
BseYI CCCAGC 1 cut(s) 572
Bsh1236I CGCG 1 cut(s) 406
BshFI GGCC 1 cut(s) 983
BsiHKAI GWGCWC 2 cut(s) 246, 348
BsiSI CCGG 1 cut(s) 315
BsiWI CGTACG 1 cut(s) 681
BslFI GGGAC 1 cut(s) 845
BslI CCNNNNNNNGG 2 cut(s) 314, 1294
BsmAI GTCTC 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 845
BsmI GAATGC 2 cut(s) 406, 1191
BsnI GGCC 1 cut(s) 983
Bsp1286I GDGCHC 2 cut(s) 246, 348
Bsp143I GATC 5 cut(s) 286, 583, 865, 1009, 1056
Bsp1720I GCTNAGC 1 cut(s) 1244
BspACI CCGC 1 cut(s) 1228
BspANI GGCC 1 cut(s) 983
BspCNI CTCAG 5 cut(s) 261, 949, 1057, 1072, 1236
BspFNI CGCG 1 cut(s) 406
BspHI TCATGA 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 368
BspMAI CTGCAG 2 cut(s) 823, 1078
BspQI GCTCTTC 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 196
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 3 cut(s) 352, 654, 1211
BssAI RCCGGY 1 cut(s) 314
BssMI GATC 5 cut(s) 286, 583, 865, 1009, 1056
Bst2UI CCWGG 1 cut(s) 549
Bst4CI ACNGT 5 cut(s) 329, 686, 707, 725, 1114
Bst6I CTCTTC 2 cut(s) 121, 489
BstAPI GCANNNNNTGC 2 cut(s) 932, 1331
BstC8I GCNNGC 5 cut(s) 840, 972, 1189, 1226, 1230
BstDEI CTNAG 6 cut(s) 97, 269, 936, 1065, 1080, 1244
BstENI CCTNNNNNAGG 1 cut(s) 1292
BstF5I GGATG 3 cut(s) 221, 244, 338
BstFNI CGCG 1 cut(s) 406
BstHHI GCGC 1 cut(s) 406
BstKTI GATC 5 cut(s) 289, 586, 868, 1012, 1059
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 5 cut(s) 286, 583, 865, 1009, 1056
BstMWI GCNNNNNNNGC 7 cut(s) 31, 412, 932, 941, 980, 1076, 1331
BstNI CCWGG 1 cut(s) 549
BstNSI RCATGY 1 cut(s) 718
BstSCI CCNGG 1 cut(s) 547
BstSFI CTRYAG 3 cut(s) 819, 1074, 1335
BstSLI GKGCMC 1 cut(s) 246
BstUI CGCG 1 cut(s) 406
BstV1I GCAGC 5 cut(s) 805, 827, 1057, 1060, 1088
BstX2I RGATCY 1 cut(s) 865
BstXI CCANNNNNNTGG 1 cut(s) 127
BstYI RGATCY 1 cut(s) 865
BsuRI GGCC 1 cut(s) 983
BtsCI GGATG 3 cut(s) 221, 244, 338
BtsIMutI CAGTG 3 cut(s) 721, 944, 1317
Cac8I GCNNGC 5 cut(s) 840, 972, 1189, 1226, 1230
CaiI CAGNNNCTG 2 cut(s) 660, 932
CciI TCATGA 1 cut(s) 229
CfoI GCGC 1 cut(s) 406
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 707
CseI GACGC 1 cut(s) 1331
Csp6I GTAC 4 cut(s) 224, 637, 682, 1132
CviAII CATG 4 cut(s) 230, 311, 437, 715
CviQI GTAC 4 cut(s) 224, 637, 682, 1132
DdeI CTNAG 6 cut(s) 97, 269, 936, 1065, 1080, 1244
DpnI GATC 5 cut(s) 288, 585, 867, 1011, 1058
DpnII GATC 5 cut(s) 286, 583, 865, 1009, 1056
EaeI YGGCCR 1 cut(s) 981
Eam1104I CTCTTC 2 cut(s) 121, 489
EarI CTCTTC 2 cut(s) 121, 489
Eco47I GGWCC 1 cut(s) 707
Eco57I CTGAAG 1 cut(s) 500
EcoNI CCTNNNNNAGG 1 cut(s) 1292
EcoRII CCWGG 1 cut(s) 547
FaeI CATG 4 cut(s) 233, 314, 440, 718
FaqI GGGAC 1 cut(s) 845
FatI CATG 4 cut(s) 229, 310, 436, 714
FauI CCCGC 1 cut(s) 1221
FauNDI CATATG 2 cut(s) 1234, 1330
FbaI TGATCA 1 cut(s) 583
Fnu4HI GCNGC 5 cut(s) 816, 819, 1071, 1074, 1077
FokI GGATG 3 cut(s) 228, 251, 325
Fsp4HI GCNGC 5 cut(s) 816, 819, 1071, 1074, 1077
FspBI CTAG 1 cut(s) 786
GlaI GCGC 1 cut(s) 405
GluI GCNGC 5 cut(s) 816, 819, 1071, 1074, 1077
GsaI CCCAGC 1 cut(s) 576
HaeIII GGCC 1 cut(s) 983
HapII CCGG 1 cut(s) 315
HgaI GACGC 1 cut(s) 1331
HhaI GCGC 1 cut(s) 406
Hin1II CATG 4 cut(s) 233, 314, 440, 718
Hin6I GCGC 1 cut(s) 404
HinP1I GCGC 1 cut(s) 404
HindIII AAGCTT 2 cut(s) 826, 836
HinfI GANTC 7 cut(s) 155, 390, 692, 929, 1118, 1280, 1298
HpaII CCGG 1 cut(s) 315
HphI GGTGA 1 cut(s) 394
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 5 cut(s) 51, 68, 270, 343, 1066
Hpy188III TCNNGA 8 cut(s) 143, 230, 374, 397, 518, 689, 696, 1196
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 4 cut(s) 65, 625, 660, 773
HpyCH4III ACNGT 5 cut(s) 329, 686, 707, 725, 1114
HpyF10VI GCNNNNNNNGC 7 cut(s) 31, 412, 932, 941, 980, 1076, 1331
HpyF3I CTNAG 6 cut(s) 97, 269, 936, 1065, 1080, 1244
Hsp92II CATG 4 cut(s) 233, 314, 440, 718
HspAI GCGC 1 cut(s) 404
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 5 cut(s) 286, 583, 865, 1009, 1056
LguI GCTCTTC 1 cut(s) 121
LmnI GCTCC 2 cut(s) 569, 1002
Lsp1109I GCAGC 5 cut(s) 805, 827, 1057, 1060, 1088
LweI GCATC 3 cut(s) 206, 889, 1109
MaeI CTAG 1 cut(s) 786
MaeIII GTNAC 5 cut(s) 317, 439, 656, 882, 1253
MalI GATC 5 cut(s) 288, 585, 867, 1011, 1058
MboI GATC 5 cut(s) 286, 583, 865, 1009, 1056
MboII GAAGA 5 cut(s) 108, 385, 506, 533, 860
MflI RGATCY 1 cut(s) 865
MhlI GDGCHC 2 cut(s) 246, 348
MlsI TGGCCA 1 cut(s) 983
MluNI TGGCCA 1 cut(s) 983
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 2 cut(s) 46, 580
MnlI CCTC 8 cut(s) 156, 318, 378, 490, 560, 1060, 1075, 1081
Mox20I TGGCCA 1 cut(s) 983
MroXI GAANNNNTTC 1 cut(s) 1173
MscI TGGCCA 1 cut(s) 983
MseI TTAA 3 cut(s) 702, 728, 780
MslI CAYNNNNRTG 3 cut(s) 125, 894, 1182
Msp20I TGGCCA 1 cut(s) 983
MspA1I CMGCKG 3 cut(s) 818, 1079, 1243
MspI CCGG 1 cut(s) 315
MspR9I CCNGG 1 cut(s) 549
Mva1269I GAATGC 2 cut(s) 406, 1191
MvaI CCWGG 1 cut(s) 549
MvnI CGCG 1 cut(s) 406
MwoI GCNNNNNNNGC 7 cut(s) 31, 412, 932, 941, 980, 1076, 1331
NdeI CATATG 2 cut(s) 1234, 1330
NdeII GATC 5 cut(s) 286, 583, 865, 1009, 1056
NlaIII CATG 4 cut(s) 233, 314, 440, 718
NlaIV GGNNCC 1 cut(s) 368
NmuCI GTSAC 2 cut(s) 439, 1253
NspI RCATGY 1 cut(s) 718
PagI TCATGA 1 cut(s) 229
PciSI GCTCTTC 1 cut(s) 121
PcsI WCGNNNNNNNCGW 1 cut(s) 286
PctI GAATGC 2 cut(s) 406, 1191
PdmI GAANNNNTTC 1 cut(s) 1173
PfeI GAWTC 6 cut(s) 390, 692, 929, 1118, 1280, 1298
Pfl23II CGTACG 1 cut(s) 681
PkrI GCNGC 5 cut(s) 817, 820, 1072, 1075, 1078
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
PsiI TTATAA 1 cut(s) 1218
Psp6I CCWGG 1 cut(s) 547
PspFI CCCAGC 1 cut(s) 572
PspGI CCWGG 1 cut(s) 547
PspLI CGTACG 1 cut(s) 681
PspN4I GGNNCC 1 cut(s) 368
PspPI GGNCC 1 cut(s) 707
PstI CTGCAG 2 cut(s) 823, 1078
PstNI CAGNNNCTG 2 cut(s) 660, 932
PsuI RGATCY 1 cut(s) 865
PvuII CAGCTG 3 cut(s) 818, 1079, 1243
RsaI GTAC 4 cut(s) 225, 638, 683, 1133
RsaNI GTAC 4 cut(s) 224, 637, 682, 1132
RseI CAYNNNNRTG 3 cut(s) 125, 894, 1182
SapI GCTCTTC 1 cut(s) 121
SaqAI TTAA 3 cut(s) 702, 728, 780
SatI GCNGC 5 cut(s) 816, 819, 1071, 1074, 1077
Sau3AI GATC 5 cut(s) 286, 583, 865, 1009, 1056
Sau96I GGNCC 1 cut(s) 707
ScaI AGTACT 1 cut(s) 225
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 549
SduI GDGCHC 2 cut(s) 246, 348
SfaNI GCATC 3 cut(s) 206, 889, 1109
SfcI CTRYAG 3 cut(s) 819, 1074, 1335
SinI GGWCC 1 cut(s) 707
SmiMI CAYNNNNRTG 3 cut(s) 125, 894, 1182
SmlI CTYRAG 1 cut(s) 1289
SmoI CTYRAG 1 cut(s) 1289
SsiI CCGC 1 cut(s) 1228
SspMI CTAG 1 cut(s) 786
StyD4I CCNGG 1 cut(s) 547
TaaI ACNGT 5 cut(s) 329, 686, 707, 725, 1114
TaqI TCGA 4 cut(s) 144, 289, 469, 697
TatI WGTACW 1 cut(s) 223
TfiI GAWTC 6 cut(s) 390, 692, 929, 1118, 1280, 1298
Tru1I TTAA 3 cut(s) 702, 728, 780
Tru9I TTAA 3 cut(s) 702, 728, 780
TscAI CASTG 3 cut(s) 728, 944, 1324
TseFI GTSAC 2 cut(s) 439, 1253
TseI GCWGC 5 cut(s) 815, 818, 1070, 1073, 1076
Tsp45I GTSAC 2 cut(s) 439, 1253
TspDTI ATGAA 9 cut(s) 218, 246, 425, 570, 786, 860, 878, 1166, 1293
TspRI CASTG 3 cut(s) 728, 944, 1324
VneI GTGCAC 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 707
XagI CCTNNNNNAGG 1 cut(s) 1292
XapI RAATTY 5 cut(s) 360, 432, 527, 620, 1032
XceI RCATGY 1 cut(s) 718
XmnI GAANNNNTTC 1 cut(s) 1173
XspI CTAG 1 cut(s) 786
ZrmI AGTACT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.