RchiOBHm_Chr5g0024391

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000364
Physical Location & Seq
Forward (+)
18548368 .. 18549505
1138 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30414

Sequence Viewer

Length: 819 bp
ATGGATTTGTCTTTCTTAGAGAGACTAGCTAAGCAGTTTGACAGAGTAGTTAAACAGTTTGAGAGACTCCCTAAGCAGTTTGGGAGACTGTTTAAACAGTTTGAGAGACTGGCTAAGCAGTTTGGAAGACTGGTTAAACAGTTTGAGAGTCTGGCTACGCAATTTGATAGAGTGTTTAAACAGTTTGAGAGACTCGCTAAGCAGTTTGAAAGACCGGTTAAACGGTTTGAGAGACTCGTTAAGAGGTTTGAGAGACTATTTAAACAGTTAGAGGGAGGAAGGATTAATCACTATAGCAGGTCCCAGAAGATACTGTTAGTGGGAGAGGGGGACTTTTCTTTTGCTCTTTGCTTGGCCAGAGCTTTCGGCTCTGCTGACAACATGGTTGCTACTTCTCTCGACTCCAAAGGGTCCTTAATGACGAACTACTCAAAAGCTATGACCAACTTGCTGGAATTAGAAGGCAGGGAATGCAAGATATTTCATAAAGTCGATGCCCGTGTCATGCGTTATCATCCTTACCTTCTCTTCATGAGGTTCCATAAGAATTTGGTCAGGAGGTTCTTCATTAGTGCACGTGAAATGCTGACTACAGATGGAGAAATTCATGTGACGCACAAGACAACATATCCTTTTAGTGAGTGGAAATTAGTGAAAATAGCAGAAGATGTTGGGTTGTATCTGGTTGGTGAAGAAAGGTTCTCACTATTTGATTATCCCGGCTATGAAAATAAGAGAGGAGCTGGGATCTGTGATCAAACTTTTCCGGTGGGAATGTCTAGCACCTTCAGATTCGCCAAGAACTACTTGTACTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

272

Amino Acids

32.21

Weight (kDa)

9.8

Isoelectric Point (pI)

35.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 105 - 171 2.5e-18 rRNA (uridine-N3-)-methyltransferase BTM5-like
BMT5-like PF10354 178 - 246 1.2e-15 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 288
AclWI GGATC 1 cut(s) 755
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 2 cut(s) 547, 603
AcuI CTGAAG 1 cut(s) 772
AcvI CACGTG 1 cut(s) 578
AfaI GTAC 1 cut(s) 812
AgeI ACCGGT 1 cut(s) 214
AgsI TTSAA 1 cut(s) 209
AluBI AGCT 4 cut(s) 29, 362, 437, 743
AluI AGCT 4 cut(s) 29, 362, 437, 743
Alw21I GWGCWC 1 cut(s) 577
Alw26I GTCTC 7 cut(s) 16, 58, 79, 100, 184, 226, 247
Alw44I GTGCAC 1 cut(s) 573
AlwI GGATC 1 cut(s) 755
AoxI GGCC 1 cut(s) 354
ApaLI GTGCAC 1 cut(s) 573
ApoI RAATTY 2 cut(s) 547, 603
ArsI GACNNNNNNTTYG 2 cut(s) 323, 355
AseI ATTAAT 1 cut(s) 285
AsiGI ACCGGT 1 cut(s) 214
AspS9I GGNCC 2 cut(s) 300, 411
AsuC2I CCSGG 1 cut(s) 720
AsuHPI GGTGA 1 cut(s) 701
AvaII GGWCC 2 cut(s) 300, 411
BaeGI GKGCMC 1 cut(s) 577
BalI TGGCCA 1 cut(s) 356
BbrPI CACGTG 1 cut(s) 578
BbsI GAAGAC 1 cut(s) 133
Bbv12I GWGCWC 1 cut(s) 577
BccI CCATC 1 cut(s) 590
BclI TGATCA 1 cut(s) 754
BcnI CCSGG 1 cut(s) 720
BcoDI GTCTC 7 cut(s) 16, 58, 79, 100, 184, 226, 247
BfaI CTAG 2 cut(s) 26, 780
BfmI CTRYAG 2 cut(s) 292, 591
BfuAI ACCTGC 1 cut(s) 288
BlpI GCTNAGC 3 cut(s) 30, 114, 198
Bme1390I CCNGG 1 cut(s) 720
Bme18I GGWCC 2 cut(s) 300, 411
BmgT120I GGNCC 2 cut(s) 300, 411
BmiI GGNNCC 3 cut(s) 302, 412, 539
BmrFI CCNGG 1 cut(s) 720
BmsI GCATC 1 cut(s) 484
BpiI GAAGAC 1 cut(s) 133
Bpu10I CCTNAGC 1 cut(s) 72
Bpu1102I GCTNAGC 3 cut(s) 30, 114, 198
BpuMI CCSGG 1 cut(s) 720
BsaAI YACGTR 1 cut(s) 578
BsaWI WCCGGW 2 cut(s) 214, 766
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 2 cut(s) 114, 135
BseGI GGATG 1 cut(s) 514
BseNI ACTGG 2 cut(s) 114, 135
BseRI GAGGAG 1 cut(s) 753
BseSI GKGCMC 1 cut(s) 577
BseYI CCCAGC 1 cut(s) 743
BshFI GGCC 1 cut(s) 356
BshTI ACCGGT 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 577
BsiSI CCGG 3 cut(s) 215, 720, 767
BslFI GGGAC 2 cut(s) 286, 344
BsmAI GTCTC 7 cut(s) 16, 58, 79, 100, 184, 226, 247
BsmFI GGGAC 2 cut(s) 286, 344
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 1 cut(s) 356
Bsp1286I GDGCHC 1 cut(s) 577
Bsp143I GATC 2 cut(s) 747, 754
Bsp1720I GCTNAGC 3 cut(s) 30, 114, 198
BspANI GGCC 1 cut(s) 356
BspHI TCATGA 1 cut(s) 531
BspLI GGNNCC 3 cut(s) 302, 412, 539
BspMI ACCTGC 1 cut(s) 288
BspPI GGATC 1 cut(s) 755
BsrFI RCCGGY 1 cut(s) 214
BsrI ACTGG 2 cut(s) 114, 135
BssAI RCCGGY 1 cut(s) 214
BssMI GATC 2 cut(s) 747, 754
Bst4CI ACNGT 9 cut(s) 57, 90, 99, 141, 183, 225, 267, 315, 815
Bst6I CTCTTC 1 cut(s) 533
BstAPI GCANNNNNTGC 1 cut(s) 471
BstBAI YACGTR 1 cut(s) 578
BstDEI CTNAG 5 cut(s) 16, 30, 72, 114, 198
BstF5I GGATG 1 cut(s) 514
BstKTI GATC 2 cut(s) 750, 757
BstMAI GTCTC 7 cut(s) 16, 58, 79, 100, 184, 226, 247
BstMBI GATC 2 cut(s) 747, 754
BstMWI GCNNNNNNNGC 1 cut(s) 471
BstSCI CCNGG 1 cut(s) 718
BstSFI CTRYAG 2 cut(s) 292, 591
BstSLI GKGCMC 1 cut(s) 577
BstV2I GAAGAC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 747
BstXI CCANNNNNNTGG 1 cut(s) 451
BstYI RGATCY 1 cut(s) 747
BsuRI GGCC 1 cut(s) 356
BtsCI GGATG 1 cut(s) 514
BveI ACCTGC 1 cut(s) 288
CciI TCATGA 1 cut(s) 531
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 2 cut(s) 300, 411
CseI GACGC 1 cut(s) 622
Csp6I GTAC 1 cut(s) 811
CspAI ACCGGT 1 cut(s) 214
CviAII CATG 4 cut(s) 382, 505, 532, 608
CviJI RGCY 9 cut(s) 29, 113, 155, 356, 362, 369, 437, 723, 743
CviKI_1 RGCY 9 cut(s) 29, 113, 155, 356, 362, 369, 437, 723, 743
CviQI GTAC 1 cut(s) 811
DdeI CTNAG 5 cut(s) 16, 30, 72, 114, 198
DpnI GATC 2 cut(s) 749, 756
DpnII GATC 2 cut(s) 747, 754
DraI TTTAAA 3 cut(s) 94, 178, 262
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 1 cut(s) 533
EarI CTCTTC 1 cut(s) 533
Eco47I GGWCC 2 cut(s) 300, 411
Eco57I CTGAAG 1 cut(s) 772
Eco72I CACGTG 1 cut(s) 578
EcoO109I RGGNCCY 2 cut(s) 300, 411
FaeI CATG 4 cut(s) 385, 508, 535, 611
FalI AAGNNNNNCTT 1 cut(s) 791
FaqI GGGAC 2 cut(s) 286, 344
FatI CATG 4 cut(s) 381, 504, 531, 607
FbaI TGATCA 1 cut(s) 754
FokI GGATG 1 cut(s) 501
FspBI CTAG 2 cut(s) 26, 780
GsaI CCCAGC 1 cut(s) 747
HaeIII GGCC 1 cut(s) 356
HapII CCGG 3 cut(s) 215, 720, 767
HgaI GACGC 1 cut(s) 622
Hin1II CATG 4 cut(s) 385, 508, 535, 611
HinfI GANTC 6 cut(s) 66, 148, 192, 234, 401, 792
HpaII CCGG 3 cut(s) 215, 720, 767
HphI GGTGA 1 cut(s) 701
Hpy166II GTNNAC 1 cut(s) 575
Hpy188I TCNGA 1 cut(s) 791
Hpy188III TCNNGA 3 cut(s) 398, 532, 556
Hpy8I GTNNAC 1 cut(s) 575
HpyAV CCTTC 4 cut(s) 273, 455, 533, 796
HpyCH4III ACNGT 9 cut(s) 57, 90, 99, 141, 183, 225, 267, 315, 815
HpyCH4IV ACGT 1 cut(s) 577
HpyCH4V TGCA 2 cut(s) 474, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 471
HpyF3I CTNAG 5 cut(s) 16, 30, 72, 114, 198
HpySE526I ACGT 1 cut(s) 577
Hsp92II CATG 4 cut(s) 385, 508, 535, 611
Ksp22I TGATCA 1 cut(s) 754
Kzo9I GATC 2 cut(s) 747, 754
LmnI GCTCC 1 cut(s) 740
LweI GCATC 1 cut(s) 484
MaeI CTAG 2 cut(s) 26, 780
MaeII ACGT 1 cut(s) 577
MaeIII GTNAC 1 cut(s) 610
MalI GATC 2 cut(s) 749, 756
MboI GATC 2 cut(s) 747, 754
MboII GAAGA 6 cut(s) 138, 319, 520, 556, 677, 704
MflI RGATCY 1 cut(s) 747
MhlI GDGCHC 1 cut(s) 577
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 5 cut(s) 161, 455, 547, 603, 647
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 5 cut(s) 60, 157, 186, 228, 395
MnlI CCTC 7 cut(s) 237, 265, 269, 319, 528, 552, 731
Mox20I TGGCCA 1 cut(s) 356
MscI TGGCCA 1 cut(s) 356
Msp20I TGGCCA 1 cut(s) 356
MspI CCGG 3 cut(s) 215, 720, 767
MspR9I CCNGG 1 cut(s) 720
MssI GTTTAAAC 2 cut(s) 94, 178
Mva1269I GAATGC 1 cut(s) 476
MwoI GCNNNNNNNGC 1 cut(s) 471
NciI CCSGG 1 cut(s) 720
NdeII GATC 2 cut(s) 747, 754
NlaIII CATG 4 cut(s) 385, 508, 535, 611
NlaIV GGNNCC 3 cut(s) 302, 412, 539
NmuCI GTSAC 1 cut(s) 610
PagI TCATGA 1 cut(s) 531
PctI GAATGC 1 cut(s) 476
PfeI GAWTC 1 cut(s) 792
PinAI ACCGGT 1 cut(s) 214
PleI GAGTC 5 cut(s) 60, 156, 186, 228, 395
PmaCI CACGTG 1 cut(s) 578
PmeI GTTTAAAC 2 cut(s) 94, 178
PmlI CACGTG 1 cut(s) 578
PpsI GAGTC 5 cut(s) 60, 156, 186, 228, 395
Ppu21I YACGTR 1 cut(s) 578
PpuMI RGGWCCY 2 cut(s) 300, 411
PshBI ATTAAT 1 cut(s) 285
Psp5II RGGWCCY 2 cut(s) 300, 411
PspCI CACGTG 1 cut(s) 578
PspFI CCCAGC 1 cut(s) 743
PspN4I GGNNCC 3 cut(s) 302, 412, 539
PspPI GGNCC 2 cut(s) 300, 411
PspPPI RGGWCCY 2 cut(s) 300, 411
PsrI GAACNNNNNNTAC 1 cut(s) 794
PsuI RGATCY 1 cut(s) 747
RsaI GTAC 1 cut(s) 812
RsaNI GTAC 1 cut(s) 811
Sau3AI GATC 2 cut(s) 747, 754
Sau96I GGNCC 2 cut(s) 300, 411
SchI GAGTC 5 cut(s) 60, 157, 186, 228, 395
ScrFI CCNGG 1 cut(s) 720
SduI GDGCHC 1 cut(s) 577
SfaNI GCATC 1 cut(s) 484
SfcI CTRYAG 2 cut(s) 292, 591
SinI GGWCC 2 cut(s) 300, 411
Sse9I AATT 5 cut(s) 161, 455, 547, 603, 647
SspMI CTAG 2 cut(s) 26, 780
StyD4I CCNGG 1 cut(s) 718
TaaI ACNGT 9 cut(s) 57, 90, 99, 141, 183, 225, 267, 315, 815
TaiI ACGT 1 cut(s) 580
TaqI TCGA 2 cut(s) 399, 492
TasI AATT 5 cut(s) 161, 455, 547, 603, 647
TatI WGTACW 1 cut(s) 810
TfiI GAWTC 1 cut(s) 792
TseFI GTSAC 1 cut(s) 610
Tsp45I GTSAC 1 cut(s) 610
TspDTI ATGAA 5 cut(s) 473, 520, 556, 596, 741
VneI GTGCAC 1 cut(s) 573
VpaK11BI GGWCC 2 cut(s) 300, 411
VspI ATTAAT 1 cut(s) 285
XapI RAATTY 2 cut(s) 547, 603
XspI CTAG 2 cut(s) 26, 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.