Rh5BG172300

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000364
Physical Location & Seq
Forward (+)
18049798 .. 18051891
2094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG172300.1

Sequence Viewer

Length: 771 bp
ATGGACTTGAGTTCTGATGAGTTTCTCTCTTGCCTTCTAATGGAAGTCCTTGGGTTTGAGAAGCGTATCAAGCACTACACCAACTATCAAAGAATACTCTTGGTCGGAGAAGGAGACTTCTCCTTTGCCGTTTCCTTAGCCAGAGCTTTCGGCTCTTCCAGAAACATGGTAGCCACTTCTCTCGACGACAGAGAGTCATTGATGAGGAAGTATTCAAAGGCTATGAGCAATGTGATGGAATTGGAGAACAGGGGATGCGTAGTACTTCATGAAGTGGATGTGCACAGAATGAGTCAACACCCTTTTCTGAGTTGTATTCGGTATGATCGAATAATCTACAATTTTCCTCATGCCGGCTACCTGCGTGGTCAATTATCATCCGAGTACAATCAGTTTCAAATTTGGTTCCATCAGGATTTGGTGAGGGGATTCTTCAAGAATGCGCGTGAAATGCTTACCGAAATAGGAGAAATTCATGTGACACACAAGACAACATATCCTTTCAGTCGATGGGAAATAGTGAAGTTAGCAGGAGAGGTTGGGTTGTATCTGCTTCAGGAAGAAAAGTTCTCAAAATGGGATTATCCAGGTTATGAAAATAAGAGAGGAGCTGGGTTATGTGATCAAACTTTTCCTGTTGGAGAATGTAGCACCTTCAAATTTGCCAAGCATTCTACCCCTGCCAGTTACTACATTGACTTGGTTCGGGCTTACGGTCAGGATTCTCAAAATAACGGTCCTTACATGCACCACTGCCATTCTTTTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

256

Amino Acids

29.8

Weight (kDa)

6.63

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 33 - 202 3.6e-54 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 369
AccII CGCG 1 cut(s) 445
AcsI RAATTY 3 cut(s) 399, 471, 659
AcuI CTGAAG 1 cut(s) 539
AfaI GTAC 2 cut(s) 264, 386
AfiI CCNNNNNNNGG 2 cut(s) 40, 353
AgsI TTSAA 4 cut(s) 216, 398, 436, 658
AhdI GACNNNNNGTC 1 cut(s) 193
AjnI CCWGG 1 cut(s) 586
AloI GAACNNNNNNTCC 2 cut(s) 551, 583
AluBI AGCT 2 cut(s) 146, 611
AluI AGCT 2 cut(s) 146, 611
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 1 cut(s) 108
Alw44I GTGCAC 1 cut(s) 281
ApaLI GTGCAC 1 cut(s) 281
ApoI RAATTY 3 cut(s) 399, 471, 659
ArsI GACNNNNNNTTYG 4 cut(s) 107, 139, 721, 753
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 1 cut(s) 737
AsuHPI GGTGA 1 cut(s) 433
AvaII GGWCC 1 cut(s) 737
BaeGI GKGCMC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 285
BccI CCATC 3 cut(s) 229, 417, 504
BceAI ACGGC 1 cut(s) 113
BciT130I CCWGG 1 cut(s) 588
BclI TGATCA 1 cut(s) 622
BcoDI GTCTC 1 cut(s) 108
BfuAI ACCTGC 1 cut(s) 369
BmcAI AGTACT 1 cut(s) 264
Bme1390I CCNGG 1 cut(s) 588
Bme18I GGWCC 1 cut(s) 737
BmeRI GACNNNNNGTC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 737
BmiI GGNNCC 1 cut(s) 407
BmrFI CCNGG 1 cut(s) 588
BmsI GCATC 1 cut(s) 245
BplI GAGNNNNNCTC 2 cut(s) 11, 43
Bpu10I CCTNAGC 1 cut(s) 136
BpuEI CTTGAG 1 cut(s) 28
BsaJI CCNNGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 353
Bse118I RCCGGY 1 cut(s) 353
Bse1I ACTGG 1 cut(s) 684
Bse3DI GCAATG 1 cut(s) 235
BseBI CCWGG 1 cut(s) 588
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 3 cut(s) 260, 283, 377
BseLI CCNNNNNNNGG 2 cut(s) 40, 353
BseMI GCAATG 1 cut(s) 235
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 1 cut(s) 684
BseRI GAGGAG 1 cut(s) 621
BseSI GKGCMC 1 cut(s) 285
BseYI CCCAGC 1 cut(s) 611
Bsh1236I CGCG 1 cut(s) 445
BsiHKAI GWGCWC 1 cut(s) 285
BsiSI CCGG 1 cut(s) 354
BslI CCNNNNNNNGG 2 cut(s) 40, 353
BsmAI GTCTC 1 cut(s) 108
BsmI GAATGC 2 cut(s) 445, 670
Bsp1286I GDGCHC 1 cut(s) 285
Bsp143I GATC 2 cut(s) 325, 622
BspCNI CTCAG 1 cut(s) 300
BspFNI CGCG 1 cut(s) 445
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 1 cut(s) 407
BspMI ACCTGC 1 cut(s) 369
BspQI GCTCTTC 1 cut(s) 160
BsrDI GCAATG 1 cut(s) 235
BsrFI RCCGGY 1 cut(s) 353
BsrI ACTGG 1 cut(s) 684
BssAI RCCGGY 1 cut(s) 353
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 2 cut(s) 325, 622
BssT1I CCWWGG 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 588
Bst4CI ACNGT 2 cut(s) 716, 737
Bst6I CTCTTC 1 cut(s) 160
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 2 cut(s) 136, 308
BstF5I GGATG 3 cut(s) 260, 283, 377
BstFNI CGCG 1 cut(s) 445
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 2 cut(s) 328, 625
BstMAI GTCTC 1 cut(s) 108
BstMBI GATC 2 cut(s) 325, 622
BstMWI GCNNNNNNNGC 2 cut(s) 70, 451
BstNI CCWGG 1 cut(s) 588
BstNSI RCATGY 1 cut(s) 748
BstSCI CCNGG 1 cut(s) 586
BstSLI GKGCMC 1 cut(s) 285
BstUI CGCG 1 cut(s) 445
BstXI CCANNNNNNTGG 1 cut(s) 166
BtsCI GGATG 3 cut(s) 260, 283, 377
BtsI GCAGTG 1 cut(s) 751
BtsIMutI CAGTG 1 cut(s) 751
BveI ACCTGC 1 cut(s) 369
Cac8I GCNNGC 1 cut(s) 355
CciI TCATGA 1 cut(s) 268
CfoI GCGC 1 cut(s) 445
Cfr10I RCCGGY 1 cut(s) 353
Cfr13I GGNCC 1 cut(s) 737
Csp6I GTAC 2 cut(s) 263, 385
CviAII CATG 5 cut(s) 166, 269, 350, 476, 745
CviJI RGCY 8 cut(s) 140, 146, 153, 173, 221, 357, 611, 710
CviKI_1 RGCY 8 cut(s) 140, 146, 153, 173, 221, 357, 611, 710
CviQI GTAC 2 cut(s) 263, 385
DdeI CTNAG 2 cut(s) 136, 308
DpnI GATC 2 cut(s) 327, 624
DpnII GATC 2 cut(s) 325, 622
DriI GACNNNNNGTC 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 160
Eam1105I GACNNNNNGTC 1 cut(s) 193
EarI CTCTTC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 49
Eco47I GGWCC 1 cut(s) 737
Eco57I CTGAAG 1 cut(s) 539
EcoRII CCWGG 1 cut(s) 586
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 5 cut(s) 169, 272, 353, 479, 748
FatI CATG 5 cut(s) 165, 268, 349, 475, 744
FbaI TGATCA 1 cut(s) 622
FokI GGATG 3 cut(s) 267, 290, 364
GlaI GCGC 1 cut(s) 444
GsaI CCCAGC 1 cut(s) 615
HapII CCGG 1 cut(s) 354
HhaI GCGC 1 cut(s) 445
Hin1II CATG 5 cut(s) 169, 272, 353, 479, 748
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HincII GTYRAC 1 cut(s) 296
HindII GTYRAC 1 cut(s) 296
HinfI GANTC 4 cut(s) 194, 292, 429, 722
HpaII CCGG 1 cut(s) 354
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 2 cut(s) 283, 296
Hpy188I TCNGA 4 cut(s) 16, 107, 309, 382
Hpy188III TCNNGA 7 cut(s) 159, 182, 269, 413, 436, 557, 719
Hpy8I GTNNAC 2 cut(s) 283, 296
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 3 cut(s) 44, 104, 664
HpyCH4III ACNGT 2 cut(s) 716, 737
HpyCH4V TGCA 2 cut(s) 283, 748
HpyF10VI GCNNNNNNNGC 2 cut(s) 70, 451
HpyF3I CTNAG 2 cut(s) 136, 308
Hsp92II CATG 5 cut(s) 169, 272, 353, 479, 748
HspAI GCGC 1 cut(s) 443
KroI GCCGGC 1 cut(s) 353
KroNI GCCGGC 1 cut(s) 355
Ksp22I TGATCA 1 cut(s) 622
Kzo9I GATC 2 cut(s) 325, 622
LguI GCTCTTC 1 cut(s) 160
LmnI GCTCC 1 cut(s) 608
LweI GCATC 1 cut(s) 245
MaeIII GTNAC 2 cut(s) 478, 686
MalI GATC 2 cut(s) 327, 624
MboI GATC 2 cut(s) 325, 622
MboII GAAGA 3 cut(s) 147, 424, 572
MhlI GDGCHC 1 cut(s) 285
MluCI AATT 6 cut(s) 239, 340, 371, 399, 471, 659
MlyI GAGTC 2 cut(s) 203, 301
MmeI TCCRAC 2 cut(s) 85, 619
MnlI CCTC 5 cut(s) 198, 357, 417, 529, 599
MroNI GCCGGC 1 cut(s) 353
MspI CCGG 1 cut(s) 354
MspR9I CCNGG 1 cut(s) 588
Mva1269I GAATGC 2 cut(s) 445, 670
MvaI CCWGG 1 cut(s) 588
MvnI CGCG 1 cut(s) 445
MwoI GCNNNNNNNGC 2 cut(s) 70, 451
NaeI GCCGGC 1 cut(s) 355
NdeII GATC 2 cut(s) 325, 622
NgoMIV GCCGGC 1 cut(s) 353
NlaIII CATG 5 cut(s) 169, 272, 353, 479, 748
NlaIV GGNNCC 1 cut(s) 407
NmuCI GTSAC 1 cut(s) 478
NspI RCATGY 1 cut(s) 748
PagI TCATGA 1 cut(s) 268
PciSI GCTCTTC 1 cut(s) 160
PcsI WCGNNNNNNNCGW 1 cut(s) 325
PctI GAATGC 2 cut(s) 445, 670
PdiI GCCGGC 1 cut(s) 355
PfeI GAWTC 2 cut(s) 429, 722
PleI GAGTC 2 cut(s) 202, 300
PpsI GAGTC 2 cut(s) 202, 300
Psp6I CCWGG 1 cut(s) 586
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 1 cut(s) 586
PspN4I GGNNCC 1 cut(s) 407
PspPI GGNCC 1 cut(s) 737
RsaI GTAC 2 cut(s) 264, 386
RsaNI GTAC 2 cut(s) 263, 385
SapI GCTCTTC 1 cut(s) 160
Sau3AI GATC 2 cut(s) 325, 622
Sau96I GGNCC 1 cut(s) 737
ScaI AGTACT 1 cut(s) 264
SchI GAGTC 2 cut(s) 203, 301
ScrFI CCNGG 1 cut(s) 588
SduI GDGCHC 1 cut(s) 285
SetI ASST 6 cut(s) 148, 363, 540, 592, 613, 656
SfaNI GCATC 1 cut(s) 245
SinI GGWCC 1 cut(s) 737
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
Sse9I AATT 6 cut(s) 239, 340, 371, 399, 471, 659
StyD4I CCNGG 1 cut(s) 586
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 2 cut(s) 716, 737
TaqI TCGA 3 cut(s) 183, 328, 508
TasI AATT 6 cut(s) 239, 340, 371, 399, 471, 659
TatI WGTACW 2 cut(s) 262, 384
TfiI GAWTC 2 cut(s) 429, 722
TscAI CASTG 1 cut(s) 758
TseFI GTSAC 1 cut(s) 478
Tsp45I GTSAC 1 cut(s) 478
TspDTI ATGAA 4 cut(s) 257, 285, 464, 609
TspRI CASTG 1 cut(s) 758
VneI GTGCAC 1 cut(s) 281
VpaK11BI GGWCC 1 cut(s) 737
XapI RAATTY 3 cut(s) 399, 471, 659
XceI RCATGY 1 cut(s) 748
ZrmI AGTACT 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.