Rh5AG174700

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000364
Physical Location & Seq
Forward (+)
19331929 .. 19333313
1385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG174700.1

Sequence Viewer

Length: 666 bp
ATGGAAGTCTTTGGCTTTGAGAAGCGTATCAAGCACTACACCAGCTATCAGACAATACTCTTGGTCGGAGAAGGAGACTTCTCCTTTGCCGTTTCCTTAGCCAGAGCTTTCGGCTCTTCCACCAAGATGATCGCTACTTCTCTCGACACCAGAGAGTCATTGAGGGAGAAGTATTCAAAGGCTATGAGCAATGTGATGGAATTGGAGAACAGGGGATGCAGAGTACTTCATGAAGTGGATGTGCACAGAATGAGCCAACACCCTTTTCTGAGTTGTATTCGGTTTGATCGAATAATCTACAATTTTCCTCATGCCGGTTACTTGCACGGTCAATCATCATCCGAGCACAACCAGTCTCAAATTTGGTTCCATCAGGATTTGGTGAGGGGATTCTGCAAGAATGCGCGTGAAATGCTTACCGAAATAGGAGAAATTCATGTGACACACAAGACAACATATCCTTTCAATCGATGGGAAATAGTTAAGTTAGCAGAAGAGGTTGGGTTGTATTTGCTTCAGGAAGAAAAGTTCTCAAAATGGGATTATCCAGGTTATGAAAATAAGAGAGGAGCTGGGTTATGTGATCAAACTTTTCCTGTTGGAGAATGTCGGAGAGTCTTCAGTACACGGCCGTTGATGTACACGGCGCATGTGCTGGGCACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

221

Amino Acids

25.7

Weight (kDa)

7.73

Isoelectric Point (pI)

51.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 20 - 189 2.6e-55 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 406
AcoI YGGCCR 1 cut(s) 629
AcsI RAATTY 2 cut(s) 360, 432
AcuI CTGAAG 2 cut(s) 500, 604
AcvI CACGTG 1 cut(s) 663
AfaI GTAC 3 cut(s) 225, 625, 641
AfiI CCNNNNNNNGG 1 cut(s) 314
AgsI TTSAA 2 cut(s) 177, 466
AjnI CCWGG 1 cut(s) 547
AjuI GAANNNNNNNTTGG 2 cut(s) 249, 281
AloI GAACNNNNNNTCC 2 cut(s) 512, 544
AluBI AGCT 3 cut(s) 45, 107, 572
AluI AGCT 3 cut(s) 45, 107, 572
Alw21I GWGCWC 2 cut(s) 246, 348
Alw26I GTCTC 2 cut(s) 69, 360
Alw44I GTGCAC 1 cut(s) 242
AoxI GGCC 1 cut(s) 629
ApaLI GTGCAC 1 cut(s) 242
ApoI RAATTY 2 cut(s) 360, 432
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AspLEI GCGC 2 cut(s) 406, 649
AsuHPI GGTGA 1 cut(s) 394
BaeGI GKGCMC 2 cut(s) 246, 662
BbrPI CACGTG 1 cut(s) 663
BbsI GAAGAC 1 cut(s) 610
Bbv12I GWGCWC 2 cut(s) 246, 348
BccI CCATC 3 cut(s) 190, 378, 465
BceAI ACGGC 4 cut(s) 74, 616, 644, 660
BciT130I CCWGG 1 cut(s) 549
BclI TGATCA 1 cut(s) 583
BcoDI GTCTC 2 cut(s) 69, 360
BmcAI AGTACT 1 cut(s) 225
Bme1390I CCNGG 1 cut(s) 549
BmiI GGNNCC 1 cut(s) 368
BmrFI CCNGG 1 cut(s) 549
BmsI GCATC 1 cut(s) 206
BpiI GAAGAC 1 cut(s) 610
Bpu10I CCTNAGC 1 cut(s) 97
Bsa29I ATCGAT 1 cut(s) 469
BsaAI YACGTR 1 cut(s) 663
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 1 cut(s) 352
Bse3DI GCAATG 1 cut(s) 196
BseBI CCWGG 1 cut(s) 549
BseCI ATCGAT 1 cut(s) 469
BseGI GGATG 3 cut(s) 221, 244, 338
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMI GCAATG 1 cut(s) 196
BseMII CTCAG 1 cut(s) 260
BseNI ACTGG 1 cut(s) 352
BseRI GAGGAG 1 cut(s) 582
BseSI GKGCMC 2 cut(s) 246, 662
BseX3I CGGCCG 1 cut(s) 629
BseYI CCCAGC 2 cut(s) 572, 655
Bsh1236I CGCG 1 cut(s) 406
Bsh1285I CGRYCG 1 cut(s) 632
BshFI GGCC 1 cut(s) 631
BshVI ATCGAT 1 cut(s) 469
BsiEI CGRYCG 1 cut(s) 632
BsiHKAI GWGCWC 2 cut(s) 246, 348
BsiSI CCGG 1 cut(s) 315
BslI CCNNNNNNNGG 1 cut(s) 314
BsmAI GTCTC 2 cut(s) 69, 360
BsmI GAATGC 1 cut(s) 406
BsnI GGCC 1 cut(s) 631
Bsp1286I GDGCHC 3 cut(s) 246, 348, 662
Bsp1407I TGTACA 1 cut(s) 639
Bsp143I GATC 3 cut(s) 129, 286, 583
BspANI GGCC 1 cut(s) 631
BspCNI CTCAG 1 cut(s) 261
BspDI ATCGAT 1 cut(s) 469
BspFNI CGCG 1 cut(s) 406
BspHI TCATGA 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 368
BspQI GCTCTTC 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 196
BsrFI RCCGGY 1 cut(s) 314
BsrGI TGTACA 1 cut(s) 639
BsrI ACTGG 1 cut(s) 352
BssAI RCCGGY 1 cut(s) 314
BssMI GATC 3 cut(s) 129, 286, 583
Bst2UI CCWGG 1 cut(s) 549
Bst4CI ACNGT 1 cut(s) 329
Bst6I CTCTTC 2 cut(s) 121, 489
BstAUI TGTACA 1 cut(s) 639
BstBAI YACGTR 1 cut(s) 663
BstDEI CTNAG 2 cut(s) 97, 269
BstF5I GGATG 3 cut(s) 221, 244, 338
BstFNI CGCG 1 cut(s) 406
BstHHI GCGC 2 cut(s) 406, 649
BstKTI GATC 3 cut(s) 132, 289, 586
BstMAI GTCTC 2 cut(s) 69, 360
BstMBI GATC 3 cut(s) 129, 286, 583
BstMCI CGRYCG 1 cut(s) 632
BstMWI GCNNNNNNNGC 2 cut(s) 31, 412
BstNI CCWGG 1 cut(s) 549
BstNSI RCATGY 1 cut(s) 653
BstSCI CCNGG 1 cut(s) 547
BstSLI GKGCMC 2 cut(s) 246, 662
BstUI CGCG 1 cut(s) 406
BstV2I GAAGAC 1 cut(s) 610
BstZI CGGCCG 1 cut(s) 629
Bsu15I ATCGAT 1 cut(s) 469
BsuRI GGCC 1 cut(s) 631
BsuTUI ATCGAT 1 cut(s) 469
BtsCI GGATG 3 cut(s) 221, 244, 338
CciI TCATGA 1 cut(s) 229
CfoI GCGC 2 cut(s) 406, 649
Cfr10I RCCGGY 1 cut(s) 314
ClaI ATCGAT 1 cut(s) 469
Csp6I GTAC 3 cut(s) 224, 624, 640
CviAII CATG 4 cut(s) 230, 311, 437, 650
CviJI RGCY 9 cut(s) 15, 45, 101, 107, 114, 182, 255, 572, 631
CviKI_1 RGCY 9 cut(s) 15, 45, 101, 107, 114, 182, 255, 572, 631
CviQI GTAC 3 cut(s) 224, 624, 640
DdeI CTNAG 2 cut(s) 97, 269
DpnI GATC 3 cut(s) 131, 288, 585
DpnII GATC 3 cut(s) 129, 286, 583
EaeI YGGCCR 1 cut(s) 629
EagI CGGCCG 1 cut(s) 629
Eam1104I CTCTTC 2 cut(s) 121, 489
EarI CTCTTC 2 cut(s) 121, 489
EclXI CGGCCG 1 cut(s) 629
Eco52I CGGCCG 1 cut(s) 629
Eco57I CTGAAG 2 cut(s) 500, 604
Eco72I CACGTG 1 cut(s) 663
EcoRII CCWGG 1 cut(s) 547
FaeI CATG 4 cut(s) 233, 314, 440, 653
FaiI YATR 8 cut(s) 185, 231, 312, 438, 457, 555, 580, 651
FatI CATG 4 cut(s) 229, 310, 436, 649
FbaI TGATCA 1 cut(s) 583
FokI GGATG 3 cut(s) 228, 251, 325
GlaI GCGC 2 cut(s) 405, 648
GsaI CCCAGC 2 cut(s) 576, 659
HaeIII GGCC 1 cut(s) 631
HapII CCGG 1 cut(s) 315
HhaI GCGC 2 cut(s) 406, 649
Hin1II CATG 4 cut(s) 233, 314, 440, 653
Hin6I GCGC 2 cut(s) 404, 647
HinP1I GCGC 2 cut(s) 404, 647
HinfI GANTC 3 cut(s) 155, 390, 615
HpaII CCGG 1 cut(s) 315
HphI GGTGA 1 cut(s) 394
Hpy166II GTNNAC 3 cut(s) 244, 626, 642
Hpy188I TCNGA 5 cut(s) 51, 68, 270, 343, 612
Hpy188III TCNNGA 4 cut(s) 143, 230, 374, 518
Hpy8I GTNNAC 3 cut(s) 244, 626, 642
HpyAV CCTTC 1 cut(s) 65
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4IV ACGT 1 cut(s) 662
HpyCH4V TGCA 4 cut(s) 219, 244, 325, 396
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 412
HpyF3I CTNAG 2 cut(s) 97, 269
HpySE526I ACGT 1 cut(s) 662
Hsp92II CATG 4 cut(s) 233, 314, 440, 653
HspAI GCGC 2 cut(s) 404, 647
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 3 cut(s) 129, 286, 583
LguI GCTCTTC 1 cut(s) 121
LmnI GCTCC 1 cut(s) 569
LweI GCATC 1 cut(s) 206
MaeII ACGT 1 cut(s) 662
MaeIII GTNAC 2 cut(s) 317, 439
MalI GATC 3 cut(s) 131, 288, 585
MboI GATC 3 cut(s) 129, 286, 583
MboII GAAGA 4 cut(s) 108, 506, 533, 610
MhlI GDGCHC 3 cut(s) 246, 348, 662
MluCI AATT 4 cut(s) 200, 301, 360, 432
MlyI GAGTC 2 cut(s) 164, 624
MmeI TCCRAC 3 cut(s) 46, 580, 590
MnlI CCTC 5 cut(s) 156, 318, 378, 490, 560
MseI TTAA 1 cut(s) 483
MslI CAYNNNNRTG 1 cut(s) 125
MspI CCGG 1 cut(s) 315
MspR9I CCNGG 1 cut(s) 549
Mva1269I GAATGC 1 cut(s) 406
MvaI CCWGG 1 cut(s) 549
MvnI CGCG 1 cut(s) 406
MwoI GCNNNNNNNGC 2 cut(s) 31, 412
NdeII GATC 3 cut(s) 129, 286, 583
NlaIII CATG 4 cut(s) 233, 314, 440, 653
NlaIV GGNNCC 1 cut(s) 368
NmuCI GTSAC 1 cut(s) 439
NspI RCATGY 1 cut(s) 653
PagI TCATGA 1 cut(s) 229
PciSI GCTCTTC 1 cut(s) 121
PcsI WCGNNNNNNNCGW 1 cut(s) 286
PctI GAATGC 1 cut(s) 406
PfeI GAWTC 1 cut(s) 390
PleI GAGTC 2 cut(s) 163, 623
PmaCI CACGTG 1 cut(s) 663
PmlI CACGTG 1 cut(s) 663
PpsI GAGTC 2 cut(s) 163, 623
Ppu21I YACGTR 1 cut(s) 663
Psp6I CCWGG 1 cut(s) 547
PspCI CACGTG 1 cut(s) 663
PspFI CCCAGC 2 cut(s) 572, 655
PspGI CCWGG 1 cut(s) 547
PspN4I GGNNCC 1 cut(s) 368
RsaI GTAC 3 cut(s) 225, 625, 641
RsaNI GTAC 3 cut(s) 224, 624, 640
RseI CAYNNNNRTG 1 cut(s) 125
SapI GCTCTTC 1 cut(s) 121
SaqAI TTAA 1 cut(s) 483
Sau3AI GATC 3 cut(s) 129, 286, 583
ScaI AGTACT 1 cut(s) 225
SchI GAGTC 2 cut(s) 164, 624
ScrFI CCNGG 1 cut(s) 549
SduI GDGCHC 3 cut(s) 246, 348, 662
SetI ASST 6 cut(s) 47, 109, 501, 553, 574, 665
SfaNI GCATC 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 125
Sse9I AATT 4 cut(s) 200, 301, 360, 432
StyD4I CCNGG 1 cut(s) 547
TaaI ACNGT 1 cut(s) 329
TaiI ACGT 1 cut(s) 665
TaqI TCGA 3 cut(s) 144, 289, 469
TasI AATT 4 cut(s) 200, 301, 360, 432
TatI WGTACW 3 cut(s) 223, 623, 639
TfiI GAWTC 1 cut(s) 390
Tru1I TTAA 1 cut(s) 483
Tru9I TTAA 1 cut(s) 483
TseFI GTSAC 1 cut(s) 439
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 4 cut(s) 218, 246, 425, 570
VneI GTGCAC 1 cut(s) 242
XapI RAATTY 2 cut(s) 360, 432
XceI RCATGY 1 cut(s) 653
ZrmI AGTACT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.