MD10G1210100.v1.1

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000364
Physical Location & Seq
Forward (+)
30897988 .. 30898907
920 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1210100.v1.1.491

Sequence Viewer

Length: 645 bp
ATGGAAGAGGGTAAACAAGAGAAACGGATAAAACATTACAGCAGTTCTCAGAGTATACTTTTAGTGGGTGAGGGTGACTTCTCCTTCGCTGCCTGCTTAGCTAAAAAATTTGGCTCTGCTGCTAGCATGATTGCCACTTCTCTCGACTCCAAAGAATTGTTGTTCTCTAAGTATTTAAGTGCAAAGAGCAACGTGAAGGAGTTAGAAGACAGGGATTGCTTAGTACTGCATGACGTGGACGTCCACACCATGAGCCTACACCCTCTCCTGATTCATAAACTATTTGATCGGATCGTGTTTAATTTTCCTCATGCTGGTTTCTTTCTTCGTGAACACAACAAGTACCAAATTGAGCTTCATCAGAATTTGGTTAGAAATTACTTCAAGAGTGCGCATAAAATGCTGGCCAACTGTGGAGAGATTCATGTGACACACAAGACTGCATATCCTTTCAATGAATGGAAGATTGTAGAGTTGGCAGAAGAATTTGGGCTGCATTTGGTTGAGGAAGCAGAGTTCTCACTATGTGATTATCCAGGTTATTTAAACAGGAGAGGAAGTGGGATCAAATGCTCTATGGAATTTCCTGTGGGAAAGTGTAGCACCTTCAACTTTGCCAAGCAATCTGCAAATAATGACCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

215

Amino Acids

24.43

Weight (kDa)

6.63

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 20 - 185 6.9e-55 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
AatII GACGTC 1 cut(s) 243
Acc16I TGCGCA 1 cut(s) 393
AccI GTMKAC 1 cut(s) 55
AclWI GGATC 2 cut(s) 299, 572
AcoI YGGCCR 1 cut(s) 405
AcsI RAATTY 4 cut(s) 107, 364, 485, 581
AcyI GRCGYC 1 cut(s) 240
AdeI CACNNNGTG 1 cut(s) 527
AfaI GTAC 2 cut(s) 225, 344
AfiI CCNNNNNNNGG 1 cut(s) 314
AgsI TTSAA 3 cut(s) 385, 454, 610
AjiI CACGTC 1 cut(s) 235
AjnI CCWGG 1 cut(s) 535
AjuI GAANNNNNNNTTGG 2 cut(s) 339, 371
AloI GAACNNNNNNTCC 2 cut(s) 500, 532
AluBI AGCT 2 cut(s) 101, 355
AluI AGCT 2 cut(s) 101, 355
AlwI GGATC 2 cut(s) 299, 572
AoxI GGCC 1 cut(s) 405
ApeKI GCWGC 3 cut(s) 89, 119, 493
ApoI RAATTY 4 cut(s) 107, 364, 485, 581
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AspLEI GCGC 1 cut(s) 394
AsuHPI GGTGA 2 cut(s) 80, 86
AsuNHI GCTAGC 1 cut(s) 122
BalI TGGCCA 1 cut(s) 407
BbsI GAAGAC 1 cut(s) 213
BbvI GCAGC 3 cut(s) 76, 106, 480
BciT130I CCWGG 1 cut(s) 537
BfaI CTAG 1 cut(s) 123
BisI GCNGC 3 cut(s) 90, 120, 494
BlpI GCTNAGC 1 cut(s) 97
BlsI GCNGC 3 cut(s) 91, 121, 495
BmcAI AGTACT 1 cut(s) 225
Bme1390I CCNGG 1 cut(s) 537
BmgBI CACGTC 1 cut(s) 235
BmrFI CCNGG 1 cut(s) 537
BmtI GCTAGC 1 cut(s) 126
BpiI GAAGAC 1 cut(s) 213
Bpu1102I GCTNAGC 1 cut(s) 97
BsaHI GRCGYC 1 cut(s) 240
BsaXI ACNNNNNCTCC 4 cut(s) 249, 279, 544, 574
Bsc4I CCNNNNNNNGG 1 cut(s) 314
BseBI CCWGG 1 cut(s) 537
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMII CTCAG 1 cut(s) 62
BseXI GCAGC 3 cut(s) 76, 106, 480
BshFI GGCC 1 cut(s) 407
BslI CCNNNNNNNGG 1 cut(s) 314
BsnI GGCC 1 cut(s) 407
Bsp143I GATC 3 cut(s) 286, 291, 564
Bsp1720I GCTNAGC 1 cut(s) 97
BspANI GGCC 1 cut(s) 407
BspCNI CTCAG 1 cut(s) 61
BspOI GCTAGC 1 cut(s) 126
BspPI GGATC 2 cut(s) 299, 572
BssMI GATC 3 cut(s) 286, 291, 564
BssNAI GTATAC 1 cut(s) 56
BssNI GRCGYC 1 cut(s) 240
Bst1107I GTATAC 1 cut(s) 56
Bst2UI CCWGG 1 cut(s) 537
Bst4CI ACNGT 1 cut(s) 413
BstACI GRCGYC 1 cut(s) 240
BstAPI GCANNNNNTGC 1 cut(s) 400
BstC8I GCNNGC 3 cut(s) 94, 124, 405
BstDEI CTNAG 4 cut(s) 48, 97, 168, 220
BstHHI GCGC 1 cut(s) 394
BstKTI GATC 3 cut(s) 289, 294, 567
BstMBI GATC 3 cut(s) 286, 291, 564
BstMWI GCNNNNNNNGC 2 cut(s) 98, 400
BstNI CCWGG 1 cut(s) 537
BstSCI CCNGG 1 cut(s) 535
BstV1I GCAGC 3 cut(s) 76, 106, 480
BstV2I GAAGAC 1 cut(s) 213
BstZ17I GTATAC 1 cut(s) 56
BsuRI GGCC 1 cut(s) 407
BtrI CACGTC 1 cut(s) 235
Cac8I GCNNGC 3 cut(s) 94, 124, 405
CfoI GCGC 1 cut(s) 394
Csp6I GTAC 2 cut(s) 224, 343
CviAII CATG 5 cut(s) 127, 230, 250, 311, 425
CviJI RGCY 6 cut(s) 101, 114, 255, 355, 407, 493
CviKI_1 RGCY 6 cut(s) 101, 114, 255, 355, 407, 493
CviQI GTAC 2 cut(s) 224, 343
DdeI CTNAG 4 cut(s) 48, 97, 168, 220
DpnI GATC 3 cut(s) 288, 293, 566
DpnII GATC 3 cut(s) 286, 291, 564
DraI TTTAAA 1 cut(s) 546
DraIII CACNNNGTG 1 cut(s) 527
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
EaeI YGGCCR 1 cut(s) 405
EcoRII CCWGG 1 cut(s) 535
FaeI CATG 5 cut(s) 130, 233, 253, 314, 428
FatI CATG 5 cut(s) 126, 229, 249, 310, 424
FblI GTMKAC 1 cut(s) 55
Fnu4HI GCNGC 3 cut(s) 90, 120, 494
Fsp4HI GCNGC 3 cut(s) 90, 120, 494
FspAI RTGCGCAY 1 cut(s) 393
FspBI CTAG 1 cut(s) 123
FspI TGCGCA 1 cut(s) 393
GlaI GCGC 1 cut(s) 393
GluI GCNGC 3 cut(s) 90, 120, 494
HaeIII GGCC 1 cut(s) 407
HhaI GCGC 1 cut(s) 394
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 5 cut(s) 130, 233, 253, 314, 428
Hin6I GCGC 1 cut(s) 392
HinP1I GCGC 1 cut(s) 392
HinfI GANTC 3 cut(s) 146, 271, 421
HphI GGTGA 2 cut(s) 80, 86
Hpy166II GTNNAC 5 cut(s) 14, 56, 238, 244, 332
Hpy188I TCNGA 3 cut(s) 51, 291, 363
Hpy188III TCNNGA 4 cut(s) 143, 268, 329, 385
Hpy8I GTNNAC 5 cut(s) 14, 56, 238, 244, 332
HpyAV CCTTC 3 cut(s) 94, 190, 616
HpyCH4III ACNGT 1 cut(s) 413
HpyCH4IV ACGT 3 cut(s) 192, 234, 240
HpyCH4V TGCA 5 cut(s) 182, 229, 443, 496, 629
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 400
HpyF3I CTNAG 4 cut(s) 48, 97, 168, 220
HpySE526I ACGT 3 cut(s) 192, 234, 240
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 5 cut(s) 130, 233, 253, 314, 428
HspAI GCGC 1 cut(s) 392
Kzo9I GATC 3 cut(s) 286, 291, 564
LpnPI CCDG 9 cut(s) 106, 196, 281, 300, 389, 522, 535, 549, 600
Lsp1109I GCAGC 3 cut(s) 76, 106, 480
MaeI CTAG 1 cut(s) 123
MaeII ACGT 3 cut(s) 192, 234, 240
MaeIII GTNAC 2 cut(s) 74, 427
MalI GATC 3 cut(s) 288, 293, 566
MboI GATC 3 cut(s) 286, 291, 564
MboII GAAGA 5 cut(s) 17, 218, 317, 475, 494
MlsI TGGCCA 1 cut(s) 407
MluCI AATT 8 cut(s) 107, 155, 301, 348, 364, 376, 485, 581
MluNI TGGCCA 1 cut(s) 407
MlyI GAGTC 1 cut(s) 140
MnlI CCTC 5 cut(s) 64, 273, 318, 499, 548
Mox20I TGGCCA 1 cut(s) 407
MscI TGGCCA 1 cut(s) 407
MseI TTAA 3 cut(s) 176, 300, 545
Msp20I TGGCCA 1 cut(s) 407
MspR9I CCNGG 1 cut(s) 537
MvaI CCWGG 1 cut(s) 537
MwoI GCNNNNNNNGC 2 cut(s) 98, 400
NdeII GATC 3 cut(s) 286, 291, 564
NheI GCTAGC 1 cut(s) 122
NlaIII CATG 5 cut(s) 130, 233, 253, 314, 428
NmuCI GTSAC 2 cut(s) 74, 427
NsbI TGCGCA 1 cut(s) 393
PfeI GAWTC 2 cut(s) 271, 421
PkrI GCNGC 3 cut(s) 91, 121, 495
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
Psp6I CCWGG 1 cut(s) 535
PspGI CCWGG 1 cut(s) 535
RsaI GTAC 2 cut(s) 225, 344
RsaNI GTAC 2 cut(s) 224, 343
SaqAI TTAA 3 cut(s) 176, 300, 545
SatI GCNGC 3 cut(s) 90, 120, 494
Sau3AI GATC 3 cut(s) 286, 291, 564
ScaI AGTACT 1 cut(s) 225
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 537
SetI ASST 7 cut(s) 103, 195, 237, 243, 357, 541, 608
Sse9I AATT 8 cut(s) 107, 155, 301, 348, 364, 376, 485, 581
SspMI CTAG 1 cut(s) 123
StyD4I CCNGG 1 cut(s) 535
TaaI ACNGT 1 cut(s) 413
TaiI ACGT 3 cut(s) 195, 237, 243
TaqI TCGA 1 cut(s) 144
TasI AATT 8 cut(s) 107, 155, 301, 348, 364, 376, 485, 581
TatI WGTACW 1 cut(s) 223
TfiI GAWTC 2 cut(s) 271, 421
Tru1I TTAA 3 cut(s) 176, 300, 545
Tru9I TTAA 3 cut(s) 176, 300, 545
TseFI GTSAC 2 cut(s) 74, 427
TseI GCWGC 3 cut(s) 89, 119, 493
Tsp45I GTSAC 2 cut(s) 74, 427
TspDTI ATGAA 4 cut(s) 263, 347, 413, 471
TspGWI ACGGA 1 cut(s) 40
XapI RAATTY 4 cut(s) 107, 364, 485, 581
XmiI GTMKAC 1 cut(s) 55
XspI CTAG 1 cut(s) 123
ZraI GACGTC 1 cut(s) 241
ZrmI AGTACT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.