MD10G1209900.v1.1

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000364
Physical Location & Seq
Forward (+)
30888058 .. 30889154
1097 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1209900.v1.1.491

Sequence Viewer

Length: 672 bp
ATGAAGATTAAGCATTACAGCAGCTTTCACAAGATACTGTTAGTGGGAGAAGGAGACTTCTCTTTTGCGCTTTGTTTGGCCAAAAGTTTCGGGTCAGCTAAGGAGTTGAAGGTGAAGTATTCAAAAGCTATGAAAAACTTGATGGAATTGGAGAACATGGGATGCAAAATCTTCCATGGGGTAGATGTACACACCATGCTCCACCACCCTCAACTTACTAGGATGGAGTTTGATGGGATCATATTTAATTTTCCTCACGCTGGATGCTCGAATTTTCGTTGTTCGTCGGAGAAAAAGCAGCACCACATTTTGTCGCACCAGAGTTTGGTGAAGGGGTACTTCAAGAGTTCAAGGGAGATGCTGACCAGAAGTGGAGAAATTCATGTGACGCACAAGACATCATATCCTTTCACTGAGTGGGAAATAGAGAATTTAGCAGAAGAGGCTGGCTTATTTTTGGTTAAAGAAGAAAAAGAGTTCTCAATAAGGGACTATCCAGATTACTTAAACAAGAGAGGTGCAGGGAAATGTGATGTATATTTTCACGTTGGAGAGGCGAGCACCTCCAGATTTCCCAAGTGCTTGTACCCTCCAGCTGCTTCCAGTTTCCGGCCCGGAGCTTTGAGTATTAGTACGTGGTCTGATGACATAGTCGATATGGCTTGCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

224

Amino Acids

25.4

Weight (kDa)

8.12

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 13 - 172 2.6e-44 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 325
AclWI GGATC 1 cut(s) 245
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 3 cut(s) 271, 378, 430
AdeI CACNNNGTG 1 cut(s) 417
AfaI GTAC 4 cut(s) 189, 338, 587, 634
AfiI CCNNNNNNNGG 3 cut(s) 260, 325, 609
AgsI TTSAA 4 cut(s) 109, 123, 343, 351
AluBI AGCT 5 cut(s) 24, 98, 128, 596, 620
AluI AGCT 5 cut(s) 24, 98, 128, 596, 620
Alw21I GWGCWC 1 cut(s) 563
Alw26I GTCTC 1 cut(s) 48
AlwI GGATC 1 cut(s) 245
AoxI GGCC 2 cut(s) 78, 611
ApeKI GCWGC 3 cut(s) 21, 298, 596
ApoI RAATTY 3 cut(s) 271, 378, 430
ArsI GACNNNNNNTTYG 2 cut(s) 47, 79
AspLEI GCGC 1 cut(s) 70
AspS9I GGNCC 1 cut(s) 612
AsuC2I CCSGG 1 cut(s) 615
AsuHPI GGTGA 2 cut(s) 124, 340
BalI TGGCCA 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 563
BbvI GCAGC 3 cut(s) 33, 310, 583
BccI CCATC 3 cut(s) 136, 217, 227
BcnI CCSGG 1 cut(s) 615
BcoDI GTCTC 1 cut(s) 48
BfaI CTAG 1 cut(s) 219
BisI GCNGC 3 cut(s) 22, 299, 597
BlsI GCNGC 3 cut(s) 23, 300, 598
Bme1390I CCNGG 1 cut(s) 615
BmgT120I GGNCC 1 cut(s) 612
BmrFI CCNGG 1 cut(s) 615
BmsI GCATC 3 cut(s) 152, 254, 348
BpmI CTGGAG 2 cut(s) 550, 576
Bpu10I CCTNAGC 1 cut(s) 99
BpuMI CCSGG 1 cut(s) 615
BsaAI YACGTR 1 cut(s) 636
BsaJI CCNNGG 1 cut(s) 175
Bsc4I CCNNNNNNNGG 3 cut(s) 260, 325, 609
Bse1I ACTGG 1 cut(s) 603
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 3 cut(s) 167, 228, 269
BseLI CCNNNNNNNGG 3 cut(s) 260, 325, 609
BseMII CTCAG 1 cut(s) 405
BseNI ACTGG 1 cut(s) 603
BseXI GCAGC 3 cut(s) 33, 310, 583
BsgI GTGCAG 1 cut(s) 540
BshFI GGCC 2 cut(s) 80, 613
BsiHKAI GWGCWC 1 cut(s) 563
BsiSI CCGG 2 cut(s) 610, 615
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 3 cut(s) 260, 325, 609
BsmAI GTCTC 1 cut(s) 48
BsmFI GGGAC 1 cut(s) 503
BsnI GGCC 2 cut(s) 80, 613
Bsp1286I GDGCHC 1 cut(s) 563
Bsp1407I TGTACA 1 cut(s) 187
Bsp143I GATC 1 cut(s) 237
Bsp19I CCATGG 1 cut(s) 175
BspANI GGCC 2 cut(s) 80, 613
BspCNI CTCAG 1 cut(s) 406
BspPI GGATC 1 cut(s) 245
BsrGI TGTACA 1 cut(s) 187
BsrI ACTGG 1 cut(s) 603
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 1 cut(s) 237
BssT1I CCWWGG 1 cut(s) 175
Bst4CI ACNGT 1 cut(s) 39
Bst6I CTCTTC 1 cut(s) 435
BstAUI TGTACA 1 cut(s) 187
BstBAI YACGTR 1 cut(s) 636
BstC8I GCNNGC 3 cut(s) 448, 559, 664
BstDEI CTNAG 2 cut(s) 99, 414
BstDSI CCRYGG 1 cut(s) 175
BstF5I GGATG 3 cut(s) 167, 228, 269
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 1 cut(s) 240
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 1 cut(s) 237
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstSCI CCNGG 1 cut(s) 613
BstV1I GCAGC 3 cut(s) 33, 310, 583
BsuRI GGCC 2 cut(s) 80, 613
BtgI CCRYGG 1 cut(s) 175
BtsCI GGATG 3 cut(s) 167, 228, 269
BtsIMutI CAGTG 1 cut(s) 411
Cac8I GCNNGC 3 cut(s) 448, 559, 664
CfoI GCGC 1 cut(s) 70
Cfr13I GGNCC 1 cut(s) 612
CseI GACGC 1 cut(s) 397
Csp6I GTAC 4 cut(s) 188, 337, 586, 633
CviAII CATG 4 cut(s) 157, 176, 196, 383
CviQI GTAC 4 cut(s) 188, 337, 586, 633
DdeI CTNAG 2 cut(s) 99, 414
DpnI GATC 1 cut(s) 239
DpnII GATC 1 cut(s) 237
DraIII CACNNNGTG 1 cut(s) 417
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
Eco130I CCWWGG 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 175
FaeI CATG 4 cut(s) 160, 179, 199, 386
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FaqI GGGAC 1 cut(s) 503
FatI CATG 4 cut(s) 156, 175, 195, 382
Fnu4HI GCNGC 3 cut(s) 22, 299, 597
FokI GGATG 3 cut(s) 174, 235, 276
Fsp4HI GCNGC 3 cut(s) 22, 299, 597
FspBI CTAG 1 cut(s) 219
GlaI GCGC 1 cut(s) 69
GluI GCNGC 3 cut(s) 22, 299, 597
GsuI CTGGAG 2 cut(s) 550, 576
HaeIII GGCC 2 cut(s) 80, 613
HapII CCGG 2 cut(s) 610, 615
HgaI GACGC 1 cut(s) 397
HhaI GCGC 1 cut(s) 70
Hin1II CATG 4 cut(s) 160, 179, 199, 386
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HpaII CCGG 2 cut(s) 610, 615
HphI GGTGA 2 cut(s) 124, 340
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 2 cut(s) 289, 643
Hpy188III TCNNGA 3 cut(s) 343, 497, 567
Hpy8I GTNNAC 1 cut(s) 190
Hpy99I CGWCG 1 cut(s) 289
HpyAV CCTTC 3 cut(s) 44, 103, 325
HpyCH4III ACNGT 1 cut(s) 39
HpyCH4IV ACGT 2 cut(s) 546, 635
HpyCH4V TGCA 3 cut(s) 165, 521, 666
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 2 cut(s) 99, 414
HpySE526I ACGT 2 cut(s) 546, 635
Hsp92II CATG 4 cut(s) 160, 179, 199, 386
HspAI GCGC 1 cut(s) 68
Kzo9I GATC 1 cut(s) 237
LmnI GCTCC 2 cut(s) 204, 617
Lsp1109I GCAGC 3 cut(s) 33, 310, 583
LweI GCATC 3 cut(s) 152, 254, 348
MaeI CTAG 1 cut(s) 219
MaeII ACGT 2 cut(s) 546, 635
MaeIII GTNAC 1 cut(s) 385
MalI GATC 1 cut(s) 239
MboI GATC 1 cut(s) 237
MboII GAAGA 4 cut(s) 16, 163, 452, 479
MhlI GDGCHC 1 cut(s) 563
MlsI TGGCCA 1 cut(s) 80
MluCI AATT 5 cut(s) 146, 247, 271, 378, 430
MluNI TGGCCA 1 cut(s) 80
MmeI TCCRAC 2 cut(s) 267, 529
MnlI CCTC 7 cut(s) 219, 264, 436, 509, 547, 574, 600
Mox20I TGGCCA 1 cut(s) 80
MscI TGGCCA 1 cut(s) 80
MseI TTAA 5 cut(s) 9, 246, 462, 506, 670
Msp20I TGGCCA 1 cut(s) 80
MspA1I CMGCKG 1 cut(s) 596
MspI CCGG 2 cut(s) 610, 615
MspR9I CCNGG 1 cut(s) 615
MwoI GCNNNNNNNGC 1 cut(s) 443
NciI CCSGG 1 cut(s) 615
NcoI CCATGG 1 cut(s) 175
NdeII GATC 1 cut(s) 237
NlaIII CATG 4 cut(s) 160, 179, 199, 386
NmuCI GTSAC 1 cut(s) 385
PflFI GACNNNGTC 1 cut(s) 650
PflMI CCANNNNNTGG 1 cut(s) 325
PkrI GCNGC 3 cut(s) 23, 300, 598
Ppu21I YACGTR 1 cut(s) 636
PspPI GGNCC 1 cut(s) 612
PsyI GACNNNGTC 1 cut(s) 650
PvuII CAGCTG 1 cut(s) 596
RsaI GTAC 4 cut(s) 189, 338, 587, 634
RsaNI GTAC 4 cut(s) 188, 337, 586, 633
SaqAI TTAA 5 cut(s) 9, 246, 462, 506, 670
SatI GCNGC 3 cut(s) 22, 299, 597
Sau3AI GATC 1 cut(s) 237
Sau96I GGNCC 1 cut(s) 612
ScrFI CCNGG 1 cut(s) 615
SduI GDGCHC 1 cut(s) 563
SfaNI GCATC 3 cut(s) 152, 254, 348
Sse9I AATT 5 cut(s) 146, 247, 271, 378, 430
SspMI CTAG 1 cut(s) 219
StyD4I CCNGG 1 cut(s) 613
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 1 cut(s) 39
TaiI ACGT 2 cut(s) 549, 638
TaqI TCGA 2 cut(s) 269, 654
TasI AATT 5 cut(s) 146, 247, 271, 378, 430
TatI WGTACW 1 cut(s) 187
Tru1I TTAA 5 cut(s) 9, 246, 462, 506, 670
Tru9I TTAA 5 cut(s) 9, 246, 462, 506, 670
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 3 cut(s) 21, 298, 596
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 3 cut(s) 17, 146, 371
TspRI CASTG 1 cut(s) 418
Tth111I GACNNNGTC 1 cut(s) 650
Van91I CCANNNNNTGG 1 cut(s) 325
XapI RAATTY 3 cut(s) 271, 378, 430
XspI CTAG 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.