pycom05g21120

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000364
Physical Location & Seq
Forward (+)
23813195 .. 23814771
1577 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21120.1

Sequence Viewer

Length: 651 bp
ATGGAAGAGGAGAAGAAGATTATGCACTACAGCAGCTCTCAGAAGATACTGTTAGTAGGTGAGGGCAACTTCTCTTTTGCTGCTTGCTTAGCCACTGCATTTGGCTCTGCAAACAACATTGTCGCCACTTCTCTCGACTCCAGAGAGTCATTTATGGAGAATTACCCAAATGCAGTGAGGAAATGGAAGAAGTTAAAGGGAAAGGGATGTGTAGTACTGCATGAAGTGGATGTGGATACCATGAGCCAACACCCACTGCTAGCTGATAAACTATTTGATCGAATCGTCTTTAATTTCCCTCACGCTGGCTTCATCGGCATGGAAAGCCAGAGGTTTCAAATTGAGTTGCATCAAGATCTGGTCAAGCGGTTCTTCGCCACTGCGTCTGAAATGCTGACGGGAAGAGGAGAAATTCATGTGACACACAAGACCAGTAATCCTTTCAGCAACTGGAACATAGTGAAGTTAGCAGAAGAGGTAGGGTTATATCTGGTTGAGGAGGCACCTTTCACGCGAGCGGATTATCCAGGTTATCTCAATAAGAAGGGAAGTGGGAGAAAATGCAACCGGACATTTCGTGTTGGACAGTGTAGTACCTACAAATTTGCCAAGCTACCACTTCAACTTCTGGTTCTTGAGTCACCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

217

Amino Acids

24.48

Weight (kDa)

8.81

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 17 - 181 8.8e-55 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 502
AccBSI CCGCTC 1 cut(s) 518
AccII CGCG 1 cut(s) 514
AciI CCGC 2 cut(s) 367, 518
AcsI RAATTY 2 cut(s) 411, 602
AfaI GTAC 2 cut(s) 216, 595
AfiI CCNNNNNNNGG 1 cut(s) 305
AgsI TTSAA 2 cut(s) 338, 623
AjnI CCWGG 1 cut(s) 526
AluBI AGCT 3 cut(s) 36, 263, 613
AluI AGCT 3 cut(s) 36, 263, 613
AlwNI CAGNNNCTG 1 cut(s) 450
ApeKI GCWGC 2 cut(s) 33, 80
ApoI RAATTY 2 cut(s) 411, 602
AsuHPI GGTGA 2 cut(s) 71, 633
AsuNHI GCTAGC 1 cut(s) 259
BanI GGYRCC 1 cut(s) 502
BbvI GCAGC 2 cut(s) 45, 67
BciT130I CCWGG 1 cut(s) 528
BciVI GTATCC 1 cut(s) 229
BfaI CTAG 1 cut(s) 260
BfmI CTRYAG 1 cut(s) 28
BfuI GTATCC 1 cut(s) 229
BglII AGATCT 1 cut(s) 355
BisI GCNGC 2 cut(s) 34, 81
BlpI GCTNAGC 1 cut(s) 88
BlsI GCNGC 2 cut(s) 35, 82
BmcAI AGTACT 1 cut(s) 216
Bme1390I CCNGG 1 cut(s) 528
BmiI GGNNCC 1 cut(s) 504
BmrFI CCNGG 1 cut(s) 528
BmsI GCATC 1 cut(s) 358
BmtI GCTAGC 1 cut(s) 263
BpmI CTGGAG 1 cut(s) 124
Bpu1102I GCTNAGC 1 cut(s) 88
BsaWI WCCGGW 1 cut(s) 567
Bsc4I CCNNNNNNNGG 1 cut(s) 305
Bse1I ACTGG 2 cut(s) 432, 455
BseBI CCWGG 1 cut(s) 528
BseGI GGATG 2 cut(s) 212, 235
BseLI CCNNNNNNNGG 1 cut(s) 305
BseMII CTCAG 1 cut(s) 53
BseNI ACTGG 2 cut(s) 432, 455
BseRI GAGGAG 3 cut(s) 23, 420, 512
BseXI GCAGC 2 cut(s) 45, 67
Bsh1236I CGCG 1 cut(s) 514
BshNI GGYRCC 1 cut(s) 502
BsiSI CCGG 1 cut(s) 568
BslI CCNNNNNNNGG 1 cut(s) 305
Bsp143I GATC 2 cut(s) 277, 355
Bsp1720I GCTNAGC 1 cut(s) 88
BspACI CCGC 2 cut(s) 367, 518
BspCNI CTCAG 1 cut(s) 52
BspFNI CGCG 1 cut(s) 514
BspLI GGNNCC 1 cut(s) 504
BspOI GCTAGC 1 cut(s) 263
BspT107I GGYRCC 1 cut(s) 502
BsrBI CCGCTC 1 cut(s) 518
BsrI ACTGG 2 cut(s) 432, 455
BssMI GATC 2 cut(s) 277, 355
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 2 cut(s) 51, 588
Bst6I CTCTTC 2 cut(s) 397, 468
BstC8I GCNNGC 4 cut(s) 85, 261, 307, 516
BstDEI CTNAG 2 cut(s) 39, 88
BstF5I GGATG 2 cut(s) 212, 235
BstFNI CGCG 1 cut(s) 514
BstKTI GATC 2 cut(s) 280, 358
BstMBI GATC 2 cut(s) 277, 355
BstMWI GCNNNNNNNGC 3 cut(s) 89, 315, 324
BstNI CCWGG 1 cut(s) 528
BstSCI CCNGG 1 cut(s) 526
BstSFI CTRYAG 1 cut(s) 28
BstUI CGCG 1 cut(s) 514
BstV1I GCAGC 2 cut(s) 45, 67
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
BsuI GTATCC 1 cut(s) 229
BtsCI GGATG 2 cut(s) 212, 235
BtsI GCAGTG 4 cut(s) 93, 180, 254, 378
BtsIMutI CAGTG 5 cut(s) 93, 180, 254, 378, 593
Cac8I GCNNGC 4 cut(s) 85, 261, 307, 516
CaiI CAGNNNCTG 1 cut(s) 450
CseI GACGC 1 cut(s) 372
Csp6I GTAC 2 cut(s) 215, 594
CspCI CAANNNNNGTGG 2 cut(s) 82, 117
CviAII CATG 4 cut(s) 221, 241, 319, 416
CviJI RGCY 8 cut(s) 36, 92, 105, 246, 263, 309, 327, 613
CviKI_1 RGCY 8 cut(s) 36, 92, 105, 246, 263, 309, 327, 613
CviQI GTAC 2 cut(s) 215, 594
DdeI CTNAG 2 cut(s) 39, 88
DpnI GATC 2 cut(s) 279, 357
DpnII GATC 2 cut(s) 277, 355
Eam1104I CTCTTC 2 cut(s) 397, 468
EarI CTCTTC 2 cut(s) 397, 468
EcoRII CCWGG 1 cut(s) 526
FaeI CATG 4 cut(s) 224, 244, 322, 419
FaiI YATR 8 cut(s) 23, 155, 222, 242, 320, 417, 458, 487
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FatI CATG 4 cut(s) 220, 240, 318, 415
Fnu4HI GCNGC 2 cut(s) 34, 81
FokI GGATG 2 cut(s) 219, 242
Fsp4HI GCNGC 2 cut(s) 34, 81
FspBI CTAG 1 cut(s) 260
GluI GCNGC 2 cut(s) 34, 81
GsuI CTGGAG 1 cut(s) 124
HapII CCGG 1 cut(s) 568
HgaI GACGC 1 cut(s) 372
Hin1II CATG 4 cut(s) 224, 244, 322, 419
HinfI GANTC 4 cut(s) 137, 146, 282, 638
HpaII CCGG 1 cut(s) 568
HphI GGTGA 2 cut(s) 71, 633
Hpy188I TCNGA 2 cut(s) 42, 388
Hpy188III TCNNGA 4 cut(s) 134, 141, 353, 635
HpyAV CCTTC 1 cut(s) 538
HpyCH4III ACNGT 2 cut(s) 51, 588
HpyCH4V TGCA 7 cut(s) 25, 98, 110, 173, 220, 349, 564
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 315, 324
HpyF3I CTNAG 2 cut(s) 39, 88
Hsp92II CATG 4 cut(s) 224, 244, 322, 419
Kzo9I GATC 2 cut(s) 277, 355
Lsp1109I GCAGC 2 cut(s) 45, 67
LweI GCATC 1 cut(s) 358
MaeI CTAG 1 cut(s) 260
MaeIII GTNAC 2 cut(s) 418, 639
MalI GATC 2 cut(s) 279, 357
MbiI CCGCTC 1 cut(s) 518
MboI GATC 2 cut(s) 277, 355
MboII GAAGA 8 cut(s) 17, 25, 28, 55, 199, 364, 414, 485
MflI RGATCY 1 cut(s) 355
MluCI AATT 5 cut(s) 160, 292, 339, 411, 602
MlyI GAGTC 3 cut(s) 131, 155, 647
MmeI TCCRAC 1 cut(s) 562
MnlI CCTC 8 cut(s) 55, 171, 309, 324, 398, 469, 490, 493
MseI TTAA 2 cut(s) 194, 291
MslI CAYNNNNRTG 1 cut(s) 317
MspI CCGG 1 cut(s) 568
MspR9I CCNGG 1 cut(s) 528
MvaI CCWGG 1 cut(s) 528
MvnI CGCG 1 cut(s) 514
MwoI GCNNNNNNNGC 3 cut(s) 89, 315, 324
NdeII GATC 2 cut(s) 277, 355
NheI GCTAGC 1 cut(s) 259
NlaIII CATG 4 cut(s) 224, 244, 322, 419
NlaIV GGNNCC 1 cut(s) 504
NmuCI GTSAC 2 cut(s) 418, 639
PfeI GAWTC 1 cut(s) 282
PkrI GCNGC 2 cut(s) 35, 82
PleI GAGTC 3 cut(s) 131, 154, 646
PpsI GAGTC 3 cut(s) 131, 154, 646
Psp6I CCWGG 1 cut(s) 526
PspGI CCWGG 1 cut(s) 526
PspN4I GGNNCC 1 cut(s) 504
PstNI CAGNNNCTG 1 cut(s) 450
PsuI RGATCY 1 cut(s) 355
RsaI GTAC 2 cut(s) 216, 595
RsaNI GTAC 2 cut(s) 215, 594
RseI CAYNNNNRTG 1 cut(s) 317
SaqAI TTAA 2 cut(s) 194, 291
SatI GCNGC 2 cut(s) 34, 81
Sau3AI GATC 2 cut(s) 277, 355
ScaI AGTACT 1 cut(s) 216
SchI GAGTC 3 cut(s) 131, 155, 647
ScrFI CCNGG 1 cut(s) 528
SfaNI GCATC 1 cut(s) 358
SfcI CTRYAG 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 317
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
Sse9I AATT 5 cut(s) 160, 292, 339, 411, 602
SsiI CCGC 2 cut(s) 367, 518
SspMI CTAG 1 cut(s) 260
StyD4I CCNGG 1 cut(s) 526
TaaI ACNGT 2 cut(s) 51, 588
TaqI TCGA 2 cut(s) 135, 280
TasI AATT 5 cut(s) 160, 292, 339, 411, 602
TatI WGTACW 1 cut(s) 214
TfiI GAWTC 1 cut(s) 282
Tru1I TTAA 2 cut(s) 194, 291
Tru9I TTAA 2 cut(s) 194, 291
TscAI CASTG 5 cut(s) 100, 180, 261, 385, 593
TseFI GTSAC 2 cut(s) 418, 639
TseI GCWGC 2 cut(s) 33, 80
Tsp45I GTSAC 2 cut(s) 418, 639
TspDTI ATGAA 3 cut(s) 237, 301, 404
TspRI CASTG 5 cut(s) 100, 180, 261, 385, 593
XapI RAATTY 2 cut(s) 411, 602
XspI CTAG 1 cut(s) 260
ZrmI AGTACT 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.