RLG00000032800

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000364
Physical Location & Seq
Forward (+)
19241382 .. 19244084
2703 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032800

Sequence Viewer

Length: 783 bp
ATGTCGTCCGATGAAGAAGAGCCATGGGAAGTGGAAGAAGTGAATGATGCTGAAAATCCAGTAGAACGTATCAAGCACTACACCAGCTATCAGACAATACTCTTGGTCGGAGAAGGAGACTTCTCCTTTGCCGTTTCCTTAGCCAGAGCTTTCGGCTCTTCCACCAAGATGATCGCTACTTCTCTCGACACCAGAGAGTCATTGAGGGAGAAGTATTCAAAGGCTATGACCAATGTGATGGAATTGAAGAACAGGGGATGCAGAGTACTTCATGAAGTGGATGTGCACAGAATGAGCCAACACCTTTTTCTGAGTTGTATTCGGTTTGATCGAATAATCTACAATTTTCCTCATGCCGGTTACTTGCACGGTCAATCATCATCCGAGCACAACCAGTTTCAAATTTGGTTCCATCAGGATTTGGTGAGGGGATTCTTCAAGAATGCGCGTGAAATGCTTACCGAAATAGGAGAAATTCATGTGACACACAAGACAACATATCCTTTCAGTCGATGGGAAATAGTGAAGTTAGCAGAAGAGGTTGGGTTGTATTTGCTTCAGGAAGAAAAGTTCTCAAAATGGGATTATCCAGGTTATGAAAATAAGAGAGGAGCTGGGTTATGTGATCAAACTTTTCCTGTTGGAGAATGTAGCACCTTCAAATTTGGGAAGCTATTGTACCATTCTACCCCTTCCAGTTACTACATTGACTTGGTTCGGGCTTACGGTCAGGATTCTCGAAATAACGGTCCTTACATGCACCACTGCCATTCTTTTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

261

Amino Acids

30.32

Weight (kDa)

6.05

Isoelectric Point (pI)

51.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 34 - 203 1.2e-55 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 448
AcsI RAATTY 3 cut(s) 402, 474, 662
AcuI CTGAAG 1 cut(s) 542
AfaI GTAC 2 cut(s) 267, 680
AfiI CCNNNNNNNGG 1 cut(s) 356
AgsI TTSAA 5 cut(s) 219, 247, 401, 439, 661
AjnI CCWGG 1 cut(s) 589
AjuI GAANNNNNNNTTGG 2 cut(s) 291, 323
AloI GAACNNNNNNTCC 2 cut(s) 554, 586
AluBI AGCT 4 cut(s) 87, 149, 614, 673
AluI AGCT 4 cut(s) 87, 149, 614, 673
Alw21I GWGCWC 2 cut(s) 288, 390
Alw26I GTCTC 1 cut(s) 111
Alw44I GTGCAC 1 cut(s) 284
ApaLI GTGCAC 1 cut(s) 284
ApoI RAATTY 3 cut(s) 402, 474, 662
ArsI GACNNNNNNTTYG 4 cut(s) 110, 142, 733, 765
AspLEI GCGC 1 cut(s) 448
AspS9I GGNCC 1 cut(s) 749
AsuHPI GGTGA 1 cut(s) 436
AvaII GGWCC 1 cut(s) 749
BaeGI GKGCMC 1 cut(s) 288
BarI GAAGNNNNNNTAC 2 cut(s) 662, 694
Bbv12I GWGCWC 2 cut(s) 288, 390
BccI CCATC 3 cut(s) 232, 420, 507
BceAI ACGGC 1 cut(s) 116
BciT130I CCWGG 1 cut(s) 591
BclI TGATCA 1 cut(s) 625
BcoDI GTCTC 1 cut(s) 111
BmcAI AGTACT 1 cut(s) 267
Bme1390I CCNGG 1 cut(s) 591
Bme18I GGWCC 1 cut(s) 749
BmgT120I GGNCC 1 cut(s) 749
BmiI GGNNCC 1 cut(s) 410
BmrFI CCNGG 1 cut(s) 591
BmsI GCATC 2 cut(s) 37, 248
Bpu10I CCTNAGC 1 cut(s) 139
BsaJI CCNNGG 1 cut(s) 23
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 3 cut(s) 59, 394, 696
BseBI CCWGG 1 cut(s) 591
BseDI CCNNGG 1 cut(s) 23
BseGI GGATG 3 cut(s) 263, 286, 380
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 3 cut(s) 59, 394, 696
BseRI GAGGAG 1 cut(s) 624
BseSI GKGCMC 1 cut(s) 288
BseYI CCCAGC 1 cut(s) 614
Bsh1236I CGCG 1 cut(s) 448
BsiHKAI GWGCWC 2 cut(s) 288, 390
BsiSI CCGG 1 cut(s) 357
BslI CCNNNNNNNGG 1 cut(s) 356
BsmAI GTCTC 1 cut(s) 111
BsmI GAATGC 1 cut(s) 448
Bsp1286I GDGCHC 2 cut(s) 288, 390
Bsp143I GATC 3 cut(s) 171, 328, 625
Bsp19I CCATGG 1 cut(s) 23
BspCNI CTCAG 1 cut(s) 303
BspFNI CGCG 1 cut(s) 448
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 410
BspQI GCTCTTC 2 cut(s) 12, 163
BsrFI RCCGGY 1 cut(s) 356
BsrI ACTGG 3 cut(s) 59, 394, 696
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 1 cut(s) 23
BssMI GATC 3 cut(s) 171, 328, 625
BssT1I CCWWGG 1 cut(s) 23
Bst2UI CCWGG 1 cut(s) 591
Bst4CI ACNGT 3 cut(s) 371, 728, 749
Bst6I CTCTTC 3 cut(s) 12, 163, 531
BstDEI CTNAG 2 cut(s) 139, 311
BstDSI CCRYGG 1 cut(s) 23
BstF5I GGATG 3 cut(s) 263, 286, 380
BstFNI CGCG 1 cut(s) 448
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 3 cut(s) 174, 331, 628
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 3 cut(s) 171, 328, 625
BstMWI GCNNNNNNNGC 1 cut(s) 454
BstNI CCWGG 1 cut(s) 591
BstNSI RCATGY 1 cut(s) 760
BstSCI CCNGG 1 cut(s) 589
BstSLI GKGCMC 1 cut(s) 288
BstUI CGCG 1 cut(s) 448
BstXI CCANNNNNNTGG 1 cut(s) 238
BtgI CCRYGG 1 cut(s) 23
BtsCI GGATG 3 cut(s) 263, 286, 380
BtsI GCAGTG 1 cut(s) 763
BtsIMutI CAGTG 1 cut(s) 763
CciI TCATGA 1 cut(s) 271
CfoI GCGC 1 cut(s) 448
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 1 cut(s) 749
Csp6I GTAC 2 cut(s) 266, 679
CviAII CATG 5 cut(s) 24, 272, 353, 479, 757
CviQI GTAC 2 cut(s) 266, 679
DdeI CTNAG 2 cut(s) 139, 311
DpnI GATC 3 cut(s) 173, 330, 627
DpnII GATC 3 cut(s) 171, 328, 625
Eam1104I CTCTTC 3 cut(s) 12, 163, 531
EarI CTCTTC 3 cut(s) 12, 163, 531
Eco130I CCWWGG 1 cut(s) 23
Eco47I GGWCC 1 cut(s) 749
Eco57I CTGAAG 1 cut(s) 542
EcoRII CCWGG 1 cut(s) 589
EcoT14I CCWWGG 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 23
FaeI CATG 5 cut(s) 27, 275, 356, 482, 760
FatI CATG 5 cut(s) 23, 271, 352, 478, 756
FbaI TGATCA 1 cut(s) 625
FokI GGATG 3 cut(s) 270, 293, 367
GlaI GCGC 1 cut(s) 447
GsaI CCCAGC 1 cut(s) 618
HapII CCGG 1 cut(s) 357
HhaI GCGC 1 cut(s) 448
Hin1II CATG 5 cut(s) 27, 275, 356, 482, 760
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HinfI GANTC 3 cut(s) 197, 432, 734
HpaII CCGG 1 cut(s) 357
HphI GGTGA 1 cut(s) 436
Hpy166II GTNNAC 1 cut(s) 286
Hpy188I TCNGA 5 cut(s) 10, 93, 110, 312, 385
Hpy188III TCNNGA 7 cut(s) 185, 272, 416, 439, 560, 731, 738
Hpy8I GTNNAC 1 cut(s) 286
HpyAV CCTTC 3 cut(s) 107, 667, 702
HpyCH4III ACNGT 3 cut(s) 371, 728, 749
HpyCH4IV ACGT 1 cut(s) 67
HpyCH4V TGCA 4 cut(s) 261, 286, 367, 760
HpyF10VI GCNNNNNNNGC 1 cut(s) 454
HpyF3I CTNAG 2 cut(s) 139, 311
HpySE526I ACGT 1 cut(s) 67
Hsp92II CATG 5 cut(s) 27, 275, 356, 482, 760
HspAI GCGC 1 cut(s) 446
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 3 cut(s) 171, 328, 625
LguI GCTCTTC 2 cut(s) 12, 163
LmnI GCTCC 1 cut(s) 611
LweI GCATC 2 cut(s) 37, 248
MaeII ACGT 1 cut(s) 67
MaeIII GTNAC 3 cut(s) 359, 481, 698
MalI GATC 3 cut(s) 173, 330, 627
MboI GATC 3 cut(s) 171, 328, 625
MboII GAAGA 8 cut(s) 26, 29, 47, 150, 259, 427, 548, 575
MhlI GDGCHC 2 cut(s) 288, 390
MluCI AATT 5 cut(s) 242, 343, 402, 474, 662
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 2 cut(s) 88, 622
MnlI CCTC 5 cut(s) 198, 360, 420, 532, 602
MslI CAYNNNNRTG 1 cut(s) 167
MspI CCGG 1 cut(s) 357
MspR9I CCNGG 1 cut(s) 591
Mva1269I GAATGC 1 cut(s) 448
MvaI CCWGG 1 cut(s) 591
MvnI CGCG 1 cut(s) 448
MwoI GCNNNNNNNGC 1 cut(s) 454
NcoI CCATGG 1 cut(s) 23
NdeII GATC 3 cut(s) 171, 328, 625
NlaIII CATG 5 cut(s) 27, 275, 356, 482, 760
NlaIV GGNNCC 1 cut(s) 410
NmuCI GTSAC 1 cut(s) 481
NspI RCATGY 1 cut(s) 760
PagI TCATGA 1 cut(s) 271
PciSI GCTCTTC 2 cut(s) 12, 163
PcsI WCGNNNNNNNCGW 1 cut(s) 328
PctI GAATGC 1 cut(s) 448
PfeI GAWTC 2 cut(s) 432, 734
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
Psp6I CCWGG 1 cut(s) 589
PspFI CCCAGC 1 cut(s) 614
PspGI CCWGG 1 cut(s) 589
PspN4I GGNNCC 1 cut(s) 410
PspPI GGNCC 1 cut(s) 749
RsaI GTAC 2 cut(s) 267, 680
RsaNI GTAC 2 cut(s) 266, 679
RseI CAYNNNNRTG 1 cut(s) 167
SapI GCTCTTC 2 cut(s) 12, 163
Sau3AI GATC 3 cut(s) 171, 328, 625
Sau96I GGNCC 1 cut(s) 749
ScaI AGTACT 1 cut(s) 267
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 1 cut(s) 591
SduI GDGCHC 2 cut(s) 288, 390
SetI ASST 9 cut(s) 70, 89, 151, 306, 543, 595, 616, 659, 675
SfaNI GCATC 2 cut(s) 37, 248
SinI GGWCC 1 cut(s) 749
SmiMI CAYNNNNRTG 1 cut(s) 167
Sse9I AATT 5 cut(s) 242, 343, 402, 474, 662
StyD4I CCNGG 1 cut(s) 589
StyI CCWWGG 1 cut(s) 23
TaaI ACNGT 3 cut(s) 371, 728, 749
TaiI ACGT 1 cut(s) 70
TaqI TCGA 4 cut(s) 186, 331, 511, 739
TasI AATT 5 cut(s) 242, 343, 402, 474, 662
TatI WGTACW 1 cut(s) 265
TfiI GAWTC 2 cut(s) 432, 734
TscAI CASTG 1 cut(s) 770
TseFI GTSAC 1 cut(s) 481
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 5 cut(s) 27, 260, 288, 467, 612
TspRI CASTG 1 cut(s) 770
VneI GTGCAC 1 cut(s) 284
VpaK11BI GGWCC 1 cut(s) 749
XapI RAATTY 3 cut(s) 402, 474, 662
XceI RCATGY 1 cut(s) 760
ZrmI AGTACT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.