MD10G1210400.v1.1

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000364
Physical Location & Seq
Forward (+)
30984126 .. 30987011
2886 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1210400.v1.1.491

Sequence Viewer

Length: 651 bp
ATGGAAGAGGAGAAAAAGATCATGCACTACAGCGGCTCTCAGAAGATACTGTTAGTAGGTGAGGGCAACTTCTCTTTTGCTGCTTGCTTAGCCACTGCATTTGCCTCTGCAAGCAACATTGTAGCCACTTCTCTCGACTCCAAAGAGTCATTTATGGAGAAGTACCCAAATGCAGTGAGGACCTTGAACGAATTAGAGGGAAAGGGATGTGTAGTATTGCATGAAGTGGATGTAGATACCATGAGCCAACACCCTCTGCTAGCCGATAAACTATTTGATCGAATAGTCTTTAATTTTCCTCATGCGGGTTTCGTCGGGATGGAAAGCCACAGGTTTCAAATTGAGTTGCATCAAGATTTGGTCAAGCGATTCTTCACCACTGCGTGTGAAATGCTGATGGCAAGAGGAGAAGTTCATGTGACTCACAAGACCGCTCATCCTTTCAGCAACTGGAACATTGTGAAGTTAGCAGAAGAGGTAGGGTTATACCTGGTTGAGGAAGCACCCTTCACGCGAGATGATTATCCAGGTTATCTCAATAAGAAAGGAAGCGGGAGAAAATGCAACCGGACATTTCGTGTTGGACAGTGTAGTACTTACAAATTTACCAAGCTACCTCTTCAACTTCTGGTTCTTGGTTCACCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

217

Amino Acids

24.31

Weight (kDa)

6.75

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 17 - 181 6.2e-55 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 434
AccII CGCG 1 cut(s) 514
AciI CCGC 4 cut(s) 33, 305, 432, 552
AcsI RAATTY 1 cut(s) 602
AdeI CACNNNGTG 1 cut(s) 384
AfaI GTAC 2 cut(s) 164, 595
AfiI CCNNNNNNNGG 2 cut(s) 305, 496
AgsI TTSAA 3 cut(s) 187, 338, 623
AjnI CCWGG 2 cut(s) 489, 526
AluBI AGCT 1 cut(s) 613
AluI AGCT 1 cut(s) 613
AlwNI CAGNNNCTG 1 cut(s) 450
ApeKI GCWGC 1 cut(s) 80
ApoI RAATTY 1 cut(s) 602
AspS9I GGNCC 1 cut(s) 180
AsuHPI GGTGA 3 cut(s) 71, 367, 633
AsuNHI GCTAGC 1 cut(s) 259
AvaII GGWCC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 67
BccI CCATC 2 cut(s) 313, 391
BciT130I CCWGG 2 cut(s) 491, 528
BfaI CTAG 1 cut(s) 260
BfmI CTRYAG 1 cut(s) 28
BisI GCNGC 2 cut(s) 34, 81
BlpI GCTNAGC 1 cut(s) 88
BlsI GCNGC 2 cut(s) 35, 82
BmcAI AGTACT 1 cut(s) 595
Bme1390I CCNGG 2 cut(s) 491, 528
Bme18I GGWCC 1 cut(s) 180
BmgT120I GGNCC 1 cut(s) 180
BmrFI CCNGG 2 cut(s) 491, 528
BmsI GCATC 1 cut(s) 358
BmtI GCTAGC 1 cut(s) 263
Bpu1102I GCTNAGC 1 cut(s) 88
BsaBI GATNNNNATC 1 cut(s) 522
BsaWI WCCGGW 1 cut(s) 567
Bsc4I CCNNNNNNNGG 2 cut(s) 305, 496
Bse1I ACTGG 1 cut(s) 455
Bse8I GATNNNNATC 1 cut(s) 522
BseBI CCWGG 2 cut(s) 491, 528
BseGI GGATG 4 cut(s) 212, 235, 324, 436
BseJI GATNNNNATC 1 cut(s) 522
BseLI CCNNNNNNNGG 2 cut(s) 305, 496
BseMII CTCAG 1 cut(s) 53
BseNI ACTGG 1 cut(s) 455
BseRI GAGGAG 2 cut(s) 23, 420
BseXI GCAGC 1 cut(s) 67
Bsh1236I CGCG 1 cut(s) 514
BsiSI CCGG 1 cut(s) 568
BslI CCNNNNNNNGG 2 cut(s) 305, 496
Bsp143I GATC 2 cut(s) 18, 277
Bsp1720I GCTNAGC 1 cut(s) 88
BspACI CCGC 4 cut(s) 33, 305, 432, 552
BspCNI CTCAG 1 cut(s) 52
BspFNI CGCG 1 cut(s) 514
BspOI GCTAGC 1 cut(s) 263
BsrBI CCGCTC 1 cut(s) 434
BsrI ACTGG 1 cut(s) 455
BssMI GATC 2 cut(s) 18, 277
Bst2UI CCWGG 2 cut(s) 491, 528
Bst4CI ACNGT 2 cut(s) 51, 588
Bst6I CTCTTC 2 cut(s) 468, 624
BstC8I GCNNGC 3 cut(s) 85, 112, 261
BstDEI CTNAG 2 cut(s) 39, 88
BstENI CCTNNNNNAGG 1 cut(s) 494
BstF5I GGATG 4 cut(s) 212, 235, 324, 436
BstFNI CGCG 1 cut(s) 514
BstKTI GATC 2 cut(s) 21, 280
BstMBI GATC 2 cut(s) 18, 277
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstNI CCWGG 2 cut(s) 491, 528
BstSCI CCNGG 2 cut(s) 489, 526
BstSFI CTRYAG 1 cut(s) 28
BstUI CGCG 1 cut(s) 514
BstV1I GCAGC 1 cut(s) 67
BtsCI GGATG 4 cut(s) 212, 235, 324, 436
BtsI GCAGTG 3 cut(s) 93, 180, 378
BtsIMutI CAGTG 4 cut(s) 93, 180, 378, 593
Cac8I GCNNGC 3 cut(s) 85, 112, 261
CaiI CAGNNNCTG 1 cut(s) 450
Cfr13I GGNCC 1 cut(s) 180
CsiI ACCWGGT 1 cut(s) 489
Csp6I GTAC 2 cut(s) 163, 594
CspCI CAANNNNNGTGG 2 cut(s) 82, 117
CviAII CATG 5 cut(s) 22, 221, 241, 302, 416
CviJI RGCY 7 cut(s) 36, 92, 125, 246, 263, 327, 613
CviKI_1 RGCY 7 cut(s) 36, 92, 125, 246, 263, 327, 613
CviQI GTAC 2 cut(s) 163, 594
DdeI CTNAG 2 cut(s) 39, 88
DpnI GATC 2 cut(s) 20, 279
DpnII GATC 2 cut(s) 18, 277
DraIII CACNNNGTG 1 cut(s) 384
Eam1104I CTCTTC 2 cut(s) 468, 624
EarI CTCTTC 2 cut(s) 468, 624
Eco47I GGWCC 1 cut(s) 180
EcoNI CCTNNNNNAGG 1 cut(s) 494
EcoO109I RGGNCCY 1 cut(s) 180
EcoRII CCWGG 2 cut(s) 489, 526
FaeI CATG 5 cut(s) 25, 224, 244, 305, 419
FaiI YATR 7 cut(s) 23, 155, 222, 242, 303, 417, 487
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FatI CATG 5 cut(s) 21, 220, 240, 301, 415
FauI CCCGC 2 cut(s) 298, 545
Fnu4HI GCNGC 2 cut(s) 34, 81
FokI GGATG 4 cut(s) 219, 242, 331, 423
Fsp4HI GCNGC 2 cut(s) 34, 81
FspBI CTAG 1 cut(s) 260
GluI GCNGC 2 cut(s) 34, 81
HapII CCGG 1 cut(s) 568
Hin1II CATG 5 cut(s) 25, 224, 244, 305, 419
HinfI GANTC 4 cut(s) 137, 146, 369, 421
HpaII CCGG 1 cut(s) 568
HphI GGTGA 3 cut(s) 71, 367, 633
Hpy166II GTNNAC 1 cut(s) 641
Hpy188I TCNGA 1 cut(s) 42
Hpy188III TCNNGA 4 cut(s) 134, 316, 353, 648
Hpy8I GTNNAC 1 cut(s) 641
Hpy99I CGWCG 1 cut(s) 317
HpyAV CCTTC 1 cut(s) 517
HpyCH4III ACNGT 2 cut(s) 51, 588
HpyCH4V TGCA 7 cut(s) 25, 98, 110, 173, 220, 349, 564
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpyF3I CTNAG 2 cut(s) 39, 88
Hsp92II CATG 5 cut(s) 25, 224, 244, 305, 419
Kzo9I GATC 2 cut(s) 18, 277
LpnPI CCDG 8 cut(s) 316, 436, 476, 503, 513, 540, 581, 614
Lsp1109I GCAGC 1 cut(s) 67
LweI GCATC 1 cut(s) 358
MabI ACCWGGT 1 cut(s) 489
MaeI CTAG 1 cut(s) 260
MaeIII GTNAC 1 cut(s) 418
MalI GATC 2 cut(s) 20, 279
MbiI CCGCTC 1 cut(s) 434
MboI GATC 2 cut(s) 18, 277
MboII GAAGA 5 cut(s) 17, 55, 364, 485, 611
MluCI AATT 4 cut(s) 191, 292, 339, 602
MlyI GAGTC 3 cut(s) 131, 155, 415
MmeI TCCRAC 1 cut(s) 562
MseI TTAA 1 cut(s) 291
MspA1I CMGCKG 1 cut(s) 33
MspI CCGG 1 cut(s) 568
MspR9I CCNGG 2 cut(s) 491, 528
MvaI CCWGG 2 cut(s) 491, 528
MvnI CGCG 1 cut(s) 514
MwoI GCNNNNNNNGC 1 cut(s) 89
NdeII GATC 2 cut(s) 18, 277
NheI GCTAGC 1 cut(s) 259
NlaIII CATG 5 cut(s) 25, 224, 244, 305, 419
NmuCI GTSAC 1 cut(s) 418
PfeI GAWTC 1 cut(s) 369
PkrI GCNGC 2 cut(s) 35, 82
PleI GAGTC 3 cut(s) 131, 154, 415
PpsI GAGTC 3 cut(s) 131, 154, 415
PpuMI RGGWCCY 1 cut(s) 180
Psp5II RGGWCCY 1 cut(s) 180
Psp6I CCWGG 2 cut(s) 489, 526
PspGI CCWGG 2 cut(s) 489, 526
PspPI GGNCC 1 cut(s) 180
PspPPI RGGWCCY 1 cut(s) 180
PstNI CAGNNNCTG 1 cut(s) 450
RsaI GTAC 2 cut(s) 164, 595
RsaNI GTAC 2 cut(s) 163, 594
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 2 cut(s) 34, 81
Sau3AI GATC 2 cut(s) 18, 277
Sau96I GGNCC 1 cut(s) 180
ScaI AGTACT 1 cut(s) 595
SchI GAGTC 3 cut(s) 131, 155, 415
ScrFI CCNGG 2 cut(s) 491, 528
SetI ASST 9 cut(s) 61, 185, 335, 480, 492, 532, 615, 619, 646
SexAI ACCWGGT 1 cut(s) 489
SfaNI GCATC 1 cut(s) 358
SfcI CTRYAG 1 cut(s) 28
SinI GGWCC 1 cut(s) 180
Sse9I AATT 4 cut(s) 191, 292, 339, 602
SsiI CCGC 4 cut(s) 33, 305, 432, 552
SspMI CTAG 1 cut(s) 260
StyD4I CCNGG 2 cut(s) 489, 526
TaaI ACNGT 2 cut(s) 51, 588
TaqI TCGA 2 cut(s) 135, 280
TasI AATT 4 cut(s) 191, 292, 339, 602
TatI WGTACW 1 cut(s) 593
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 1 cut(s) 369
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 4 cut(s) 100, 180, 385, 593
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 1 cut(s) 80
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 2 cut(s) 237, 404
TspRI CASTG 4 cut(s) 100, 180, 385, 593
VpaK11BI GGWCC 1 cut(s) 180
XagI CCTNNNNNAGG 1 cut(s) 494
XapI RAATTY 1 cut(s) 602
XspI CTAG 1 cut(s) 260
ZrmI AGTACT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.