Rorug05G0083300

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000364
Physical Location & Seq
Reverse (-)
7212860 .. 7216776
3917 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0083300.1

Sequence Viewer

Length: 3246 bp
ATGGAGAGAATTACTTGGTTCTTCCTGGCCGCGGCTAGCTGCTTAACTATGGCAGCAGCACTAACCAACAGTAATCTCACTACAGATGAGTCTGCTCTTCTTGCTCTCAAAGCTCATATCACCAGTGATCCTCATAACATGATCTCCACCAACTGGTCAACCACCACCCCTGTTTGCAACTGGGTTGGTGTTACTTGTGGTGCACGCCACTTTAGAGTGGCAGTCTTGAACCTCTCTTATATGGGACTCACGAGCACCATTCCTCCGGAGCTTGGTAACCTATCGTTCCTTGTTGCGCTAAGCTTGAGAAATAACAGTTTTCATGGTACCTTGCCTTTGGAATTGGGTCGTTTGCGCCGATTGAAGCTCATCAGTTTCAGATTCAACAATTTTATGGGAGCCATTCCATCATGGTTTGGGTCCTTATCCAAACTTGAAACCTTCGATTTGTATGGTAATCGATTTTCAGGCTCCATACCTGCTGCCATCTTCAACTTAACAGCTCTCCAAGTAATCAACCTGAGCTATAACCAGCTATCAGGTAGCATACCAAGAGAAATTGGAAACCTAACAAAGTTGAAGGAGATATACCTTGATAGAAATAATTTCAAAGAAATTCCAAACGAGATTGGAGCCTTGAATCAACTGGAGAGGTTGTTTGTGCAGTTCAATGCCTTAGAAGGGCATGTTCCTCTCGCTGTCTTCAACATGTCTTCTTTGATTACTATGACTTTCTATAGAAACAGTTTGAATGGTAGTCTTCCAGAGAATATATATCAACATCTTCCGAGCATTCAACAGTTGGATTTGGCTCAGAACCAGATTGATGGTCCAATTCCATCCAATATTGGCAACTTAACCCAGATAAGGATGATTGACCTTGGCTGGAACTATTTGACAGGGACTATACCACATGAGATTGGTCATCTTCCAAGTTTAGAGAGATTGAGCCTCGGAATTAATAATCTCAGTGGTCTCATCCCATCCTCGATCTTCAATATTTCCACAATAACATTGATAGATCTTCACGACAATCAGCTCTCAGGCACCCTTCCAGCAAACATAGGCCTTGGGCTTCCAAACCTCCAATTTTTCTTCATAGGAGGAAATAAGCTCAGTGGAGTACTCCCTAAATCCATCTCCAATGCTTCTCAGCTCATAAGGCTAGAGCTTGGCCAAAACTTATTTTCCGGCTTTATTCCTAGTGAGCTCTGTGCCTTAGCAAACCTTTATCGGCTTGCCTTACACCAGAATAATTTGACCATTGATACTTCTATTCCAGAAGTAAATTTTTTCTCTTGTTTGGCAAATCTTAAAGATCTGAGCACATTAGCCTTGTCACACAATCCTTTAAATGCCAAGCTTTCGGTTTCCTTTAGTAATCTCTCAGCATCACTTGAATTCATTTATTTAGGTGACTGCAACATGAGAGGTAACATCCCCAATGATATAGGCAACTTGAGCAACTTAACACTCTTAGACCTTGCGGAAAATAAACTGAGTGGACCAGTTCCAACTTCAATGGGAAGATTACAGAATGTCCAAATTTTGGATGTGCATGGTAATAAATTGCAAGGACACATTCCGGATGAACTTTGTCAACTAATCCATCTAAATGAGTTAAGTCTGTATGGGAATCAACTTACTGGTGCCATACCTGCCTGCTTGGGTGATCTAAATGCAGCTCTAAGAGGTCTATTCTTACAGTCCAATTTGTTAACATCCACAATACCATCTACCTTGTGGGGGCTTTCAGATATCTTGCACTTAAACCTTTCATCCAACTCTCTAGTTGGGCCTCTCTCAGAAGGTTTTGGCAATTTGAAAGTTGTCATCGATGTAGATTTATCAAACAACCAACTATCTGGCAGCATACCAAGCAGTATTCAGAGTCTACAAAATTTGGTCAATCTGTCCTTGGCAAGTAATAATTTAGAAGGCCCTATTCCTAGTTCCTTTGGAAACTTGCTAAGCTTAGTACGCTTGGATTTATCCCAGAACAATCTCTCTGGAATGATTCCAAAGTCTCTAGAAGCACTCTCATATCTCAAGTATCTGAACTTGTCTTTCAACAAACTCCAAGGAGAAATTCCAACAGGCGGACCTTTCACAAACTTGTCTGCTCAATCGTTTGTTTCAAACCGTGGACTCTGTGGTGTATCCCGATTTCATGTTCAACCATGCAAAAGGAAAAGTGATACATCTATCTTGAAATATGTTATTCCAGGGATCTTGTCAGCAATGCTTCTTGTGATCTCCATAATTTGGTTGGTGATGTTACGCAGAAAAAAGAATGTGGAAGCTACAATAGAGACTACCTACTTGCCTCAACCTCTGTACAGAAGAGTTTCATACCAAGAGCTTCTAAGTGCAACAAATGGATTCAATGTGAGCAACTTACTTGGCACTGGCAGTTTTGGCTCAGTTTATAAAGGAACATTTTCAGATGGGGTAGATGTGGCCATAAAGGTTTTTAATTTAGAAACAGAAGGGGCTTTCACAAGTTTTGATGTTGAATGTGACATGCTAAGCAATATTCGTCACCGAAATCTTATCAAAGTCATCAGTTGCTGCAATCAAAATGATTTCAAAGCTCTTATACTGAATTACATGCCTAATGGGAACCTGGAGAAGTGGTTATATTCACAGAACTATTCTTTGAACATGCTGCAGAGGTTGGATATAATGATAAATGTTGCATCAGCTTTGGAGTATCTTCATCACGATTACGAAACACCTATTGTCCATTGTGACTTGAAGCCCAGCAACATATTACTAGATGATGATATGGTTGCACATGTTGCTGACTTTGGCATAGCAAAACTATTAGGCGGAGGAGATTCTATGACTCAAACGATGACGCTGGCGACAATTGGGTATATGGCTCCAGAGTACGGAACGGAAGGGATTGTCACTAGAAGAGGGGATGTGTATAGTTTTGGCATTGTATTGATGGAAACATTCACAAGAAGGAAGCCAACTGATGAGATGTTCGTTGGGGAAATGAGTTTAAAGCAATGGGTTGCAAATTCACTATTTGCAGATGCAATAGTTGAAGTTGTTGATACTAATTTACTTGGGACACAGAAAGATCCTAAATTTGTGAGCTATAATGTTTGCTTATCATCCATTATGAGATTAGGTGTTGCTTGTTGTGTAGAGACACCAGAAGAGAGGATAAATATGCAAGAGGCTGTAGCCACGCTGAACAAAATCAAGACCGAGTATGTGAAGGAGGTGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

1081

Amino Acids

118.86

Weight (kDa)

5.87

Isoelectric Point (pI)

31.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 66 1.4e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 76 - 178 3e-08 Leucine-rich repeat region
LRR_8 PF13855 120 - 179 9.1e-08 Leucine rich repeat
LRR_14 PF23598 163 - 244 3e-07 Leucine-rich repeat region
LRR_14 PF23598 464 - 559 9.2e-07 Leucine-rich repeat region
LRR_14 PF23598 579 - 693 5.5e-07 Leucine-rich repeat region
LRR_8 PF13855 634 - 693 1.9e-08 Leucine rich repeat
Pkinase PF00069 796 - 1067 8.8e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 797 - 1066 4.3e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2430
Acc36I ACCTGC 2 cut(s) 487, 1666
Acc65I GGTACC 1 cut(s) 326
AccB1I GGYRCC 3 cut(s) 326, 1046, 1649
AccB7I CCANNNNNTGG 3 cut(s) 153, 1549, 2265
AccI GTMKAC 1 cut(s) 1894
AccII CGCG 1 cut(s) 32
AccIII TCCGGA 2 cut(s) 265, 1585
AciI CCGC 5 cut(s) 30, 32, 1487, 2100, 2832
AclWI GGATC 3 cut(s) 122, 2237, 3086
AcoI YGGCCR 3 cut(s) 27, 1174, 2460
AcsI RAATTY 8 cut(s) 615, 1288, 1400, 1545, 1900, 2088, 3028, 3098
AfaI GTAC 5 cut(s) 328, 1125, 1980, 2339, 2894
AflIII ACRYGT 2 cut(s) 708, 2797
AjnI CCWGG 3 cut(s) 24, 2224, 2625
AjuI GAANNNNNNNTTGG 2 cut(s) 2073, 2105
Alw21I GWGCWC 4 cut(s) 205, 257, 1212, 1328
Alw26I GTCTC 4 cut(s) 980, 2031, 2306, 3155
Alw44I GTGCAC 1 cut(s) 201
AlwI GGATC 3 cut(s) 122, 2237, 3086
AlwNI CAGNNNCTG 1 cut(s) 2571
Aor13HI TCCGGA 2 cut(s) 265, 1585
AoxI GGCC 6 cut(s) 27, 1066, 1174, 1796, 1939, 2460
ApaLI GTGCAC 1 cut(s) 201
ApeKI GCWGC 8 cut(s) 39, 53, 56, 482, 1682, 1869, 2571, 2668
ApoI RAATTY 8 cut(s) 615, 1288, 1400, 1545, 1900, 2088, 3028, 3098
AseI ATTAAT 1 cut(s) 960
Asp700I GAANNNNTTC 2 cut(s) 2347, 2440
Asp718I GGTACC 1 cut(s) 326
AspLEI GCGC 2 cut(s) 298, 357
AspS9I GGNCC 6 cut(s) 420, 830, 1505, 1796, 1940, 2102
AsuHPI GGTGA 5 cut(s) 112, 1427, 1682, 2284, 2534
AsuNHI GCTAGC 1 cut(s) 35
AvaII GGWCC 4 cut(s) 420, 830, 1505, 2102
BaeGI GKGCMC 1 cut(s) 205
BalI TGGCCA 2 cut(s) 1176, 2462
BanI GGYRCC 3 cut(s) 326, 1046, 1649
BanII GRGCYC 1 cut(s) 1212
BarI GAAGNNNNNNTAC 2 cut(s) 572, 604
BauI CACGAG 1 cut(s) 250
BbsI GAAGAC 3 cut(s) 694, 705, 752
Bbv12I GWGCWC 4 cut(s) 205, 257, 1212, 1328
BbvI GCAGC 8 cut(s) 26, 65, 68, 469, 1694, 1881, 2558, 2655
BciT130I CCWGG 3 cut(s) 26, 2226, 2627
BciVI GTATCC 1 cut(s) 2170
BcoDI GTCTC 4 cut(s) 980, 2031, 2306, 3155
BfaI CTAG 8 cut(s) 36, 1166, 1203, 1790, 1950, 2030, 2777, 2916
BfmI CTRYAG 4 cut(s) 81, 736, 2669, 3195
BfuAI ACCTGC 2 cut(s) 487, 1666
BfuI GTATCC 1 cut(s) 2170
BglII AGATCT 2 cut(s) 1021, 1318
BlpI GCTNAGC 3 cut(s) 299, 1970, 2528
BmcAI AGTACT 1 cut(s) 1125
Bme1390I CCNGG 3 cut(s) 26, 2226, 2627
Bme18I GGWCC 4 cut(s) 420, 830, 1505, 2102
BmgT120I GGNCC 6 cut(s) 420, 830, 1505, 1796, 1940, 2102
BmiI GGNNCC 9 cut(s) 328, 400, 421, 472, 634, 1048, 1651, 2624, 2886
BmrFI CCNGG 3 cut(s) 26, 2226, 2627
BmrI ACTGGG 1 cut(s) 190
BmsI GCATC 3 cut(s) 1400, 2708, 3034
BmtI GCTAGC 1 cut(s) 39
BmuI ACTGGG 1 cut(s) 190
BpiI GAAGAC 3 cut(s) 694, 705, 752
BpmI CTGGAG 3 cut(s) 668, 2648, 2871
Bpu10I CCTNAGC 2 cut(s) 521, 1219
Bpu1102I GCTNAGC 3 cut(s) 299, 1970, 2528
BpuEI CTTGAG 3 cut(s) 325, 1480, 2033
Bsa29I ATCGAT 2 cut(s) 460, 1836
BsaI GGTCTC 1 cut(s) 980
BsaJI CCNNGG 8 cut(s) 30, 880, 952, 1069, 1917, 2080, 2143, 2225
BsaWI WCCGGW 2 cut(s) 265, 1585
BsaXI ACNNNNNCTCC 9 cut(s) 128, 158, 247, 277, 641, 671, 2621, 2651, 3227
Bse1I ACTGG 7 cut(s) 123, 158, 185, 651, 1508, 1651, 2413
Bse3DI GCAATG 2 cut(s) 2247, 3023
BseAI TCCGGA 2 cut(s) 265, 1585
BseBI CCWGG 3 cut(s) 26, 2226, 2627
BseCI ATCGAT 2 cut(s) 460, 1836
BseDI CCNNGG 8 cut(s) 30, 880, 952, 1069, 1917, 2080, 2143, 2225
BseMI GCAATG 2 cut(s) 2247, 3023
BseNI ACTGG 7 cut(s) 123, 158, 185, 651, 1508, 1651, 2413
BseRI GAGGAG 1 cut(s) 2850
BseSI GKGCMC 1 cut(s) 205
BseXI GCAGC 8 cut(s) 26, 65, 68, 469, 1694, 1881, 2558, 2655
BseYI CCCAGC 1 cut(s) 2762
BsgI GTGCAG 1 cut(s) 683
Bsh1236I CGCG 1 cut(s) 32
BshFI GGCC 6 cut(s) 29, 1068, 1176, 1798, 1941, 2462
BshNI GGYRCC 3 cut(s) 326, 1046, 1649
BshVI ATCGAT 2 cut(s) 460, 1836
BsiHKAI GWGCWC 4 cut(s) 205, 257, 1212, 1328
BsiSI CCGG 3 cut(s) 266, 1191, 1586
BslFI GGGAC 3 cut(s) 258, 916, 3094
BsmAI GTCTC 4 cut(s) 980, 2031, 2306, 3155
BsmFI GGGAC 3 cut(s) 258, 916, 3094
BsmI GAATGC 1 cut(s) 792
BsnI GGCC 6 cut(s) 29, 1068, 1176, 1798, 1941, 2462
Bso31I GGTCTC 1 cut(s) 980
Bsp1286I GDGCHC 4 cut(s) 205, 257, 1212, 1328
Bsp13I TCCGGA 2 cut(s) 265, 1585
Bsp1407I TGTACA 1 cut(s) 2337
Bsp143I GATC 9 cut(s) 127, 141, 990, 1021, 1318, 1672, 2229, 2253, 3091
Bsp1720I GCTNAGC 3 cut(s) 299, 1970, 2528
BspACI CCGC 5 cut(s) 30, 32, 1487, 2100, 2832
BspANI GGCC 6 cut(s) 29, 1068, 1176, 1798, 1941, 2462
BspDI ATCGAT 2 cut(s) 460, 1836
BspEI TCCGGA 2 cut(s) 265, 1585
BspFNI CGCG 1 cut(s) 32
BspLI GGNNCC 9 cut(s) 328, 400, 421, 472, 634, 1048, 1651, 2624, 2886
BspMAI CTGCAG 1 cut(s) 2673
BspMI ACCTGC 2 cut(s) 487, 1666
BspOI GCTAGC 1 cut(s) 39
BspPI GGATC 3 cut(s) 122, 2237, 3086
BspQI GCTCTTC 1 cut(s) 102
BspT107I GGYRCC 3 cut(s) 326, 1046, 1649
BspTNI GGTCTC 1 cut(s) 980
BsrDI GCAATG 2 cut(s) 2247, 3023
BsrGI TGTACA 1 cut(s) 2337
BsrI ACTGG 7 cut(s) 123, 158, 185, 651, 1508, 1651, 2413
BssECI CCNNGG 8 cut(s) 30, 880, 952, 1069, 1917, 2080, 2143, 2225
BssMI GATC 9 cut(s) 127, 141, 990, 1021, 1318, 1672, 2229, 2253, 3091
BssSI CACGAG 1 cut(s) 250
BssT1I CCWWGG 4 cut(s) 880, 1069, 1917, 2080
Bst2BI CACGAG 1 cut(s) 250
Bst2UI CCWGG 3 cut(s) 26, 2226, 2627
Bst4CI ACNGT 6 cut(s) 71, 317, 746, 801, 1707, 2144
Bst6I CTCTTC 4 cut(s) 102, 2338, 2914, 3165
BstAPI GCANNNNNTGC 1 cut(s) 2801
BstAUI TGTACA 1 cut(s) 2337
BstC8I GCNNGC 5 cut(s) 37, 205, 1239, 1663, 2865
BstDSI CCRYGG 2 cut(s) 30, 2143
BstEII GGTNACC 1 cut(s) 275
BstFNI CGCG 1 cut(s) 32
BstHHI GCGC 2 cut(s) 298, 357
BstKTI GATC 9 cut(s) 130, 144, 993, 1024, 1321, 1675, 2232, 2256, 3094
BstMAI GTCTC 4 cut(s) 980, 2031, 2306, 3155
BstMBI GATC 9 cut(s) 127, 141, 990, 1021, 1318, 1672, 2229, 2253, 3091
BstMWI GCNNNNNNNGC 9 cut(s) 101, 110, 1162, 1461, 1658, 1878, 1980, 2418, 2801
BstNI CCWGG 3 cut(s) 26, 2226, 2627
BstNSI RCATGY 6 cut(s) 689, 712, 2527, 2614, 2668, 2801
BstPI GGTNACC 1 cut(s) 275
BstSCI CCNGG 3 cut(s) 24, 2224, 2625
BstSFI CTRYAG 4 cut(s) 81, 736, 2669, 3195
BstSLI GKGCMC 1 cut(s) 205
BstUI CGCG 1 cut(s) 32
BstV1I GCAGC 8 cut(s) 26, 65, 68, 469, 1694, 1881, 2558, 2655
BstV2I GAAGAC 3 cut(s) 694, 705, 752
BstX2I RGATCY 4 cut(s) 1021, 1318, 2229, 3091
BstXI CCANNNNNNTGG 2 cut(s) 827, 1865
BstYI RGATCY 4 cut(s) 1021, 1318, 2229, 3091
Bsu15I ATCGAT 2 cut(s) 460, 1836
BsuI GTATCC 1 cut(s) 2170
BsuRI GGCC 6 cut(s) 29, 1068, 1176, 1798, 1941, 2462
BsuTUI ATCGAT 2 cut(s) 460, 1836
BtgI CCRYGG 2 cut(s) 30, 2143
BtsIMutI CAGTG 4 cut(s) 130, 976, 1123, 2406
BveI ACCTGC 2 cut(s) 487, 1666
Cac8I GCNNGC 5 cut(s) 37, 205, 1239, 1663, 2865
CaiI CAGNNNCTG 1 cut(s) 2571
CfoI GCGC 2 cut(s) 298, 357
Cfr13I GGNCC 6 cut(s) 420, 830, 1505, 1796, 1940, 2102
Cfr42I CCGCGG 1 cut(s) 33
ClaI ATCGAT 2 cut(s) 460, 1836
CseI GACGC 1 cut(s) 2869
Csp6I GTAC 5 cut(s) 327, 1124, 1979, 2338, 2893
CviQI GTAC 5 cut(s) 327, 1124, 1979, 2338, 2893
DpnI GATC 9 cut(s) 129, 143, 992, 1023, 1320, 1674, 2231, 2255, 3093
DpnII GATC 9 cut(s) 127, 141, 990, 1021, 1318, 1672, 2229, 2253, 3091
DraI TTTAAA 2 cut(s) 1353, 3012
EaeI YGGCCR 3 cut(s) 27, 1174, 2460
Eam1104I CTCTTC 4 cut(s) 102, 2338, 2914, 3165
EarI CTCTTC 4 cut(s) 102, 2338, 2914, 3165
EciI GGCGGA 2 cut(s) 2115, 2847
Ecl136II GAGCTC 1 cut(s) 1210
Eco130I CCWWGG 4 cut(s) 880, 1069, 1917, 2080
Eco147I AGGCCT 1 cut(s) 1068
Eco24I GRGCYC 1 cut(s) 1212
Eco31I GGTCTC 1 cut(s) 980
Eco32I GATATC 1 cut(s) 1759
Eco47I GGWCC 4 cut(s) 420, 830, 1505, 2102
Eco53kI GAGCTC 1 cut(s) 1210
Eco91I GGTNACC 1 cut(s) 275
EcoICRI GAGCTC 1 cut(s) 1210
EcoO109I RGGNCCY 2 cut(s) 420, 1940
EcoO65I GGTNACC 1 cut(s) 275
EcoRI GAATTC 1 cut(s) 1400
EcoRII CCWGG 3 cut(s) 24, 2224, 2625
EcoRV GATATC 1 cut(s) 1759
EcoT14I CCWWGG 4 cut(s) 880, 1069, 1917, 2080
EcoT38I GRGCYC 1 cut(s) 1212
ErhI CCWWGG 4 cut(s) 880, 1069, 1917, 2080
FaqI GGGAC 3 cut(s) 258, 916, 3094
FblI GTMKAC 1 cut(s) 1894
FriOI GRGCYC 1 cut(s) 1212
FspBI CTAG 8 cut(s) 36, 1166, 1203, 1790, 1950, 2030, 2777, 2916
GlaI GCGC 2 cut(s) 297, 356
GsaI CCCAGC 1 cut(s) 2766
GsuI CTGGAG 3 cut(s) 668, 2648, 2871
HaeIII GGCC 6 cut(s) 29, 1068, 1176, 1798, 1941, 2462
HapII CCGG 3 cut(s) 266, 1191, 1586
HgaI GACGC 1 cut(s) 2869
HhaI GCGC 2 cut(s) 298, 357
Hin6I GCGC 2 cut(s) 296, 355
HinP1I GCGC 2 cut(s) 296, 355
HincII GTYRAC 3 cut(s) 159, 1601, 1719
HindII GTYRAC 3 cut(s) 159, 1601, 1719
HindIII AAGCTT 3 cut(s) 301, 1361, 1972
HpaI GTTAAC 1 cut(s) 1719
HpaII CCGG 3 cut(s) 266, 1191, 1586
HphI GGTGA 5 cut(s) 112, 1427, 1682, 2284, 2534
Hpy166II GTNNAC 7 cut(s) 159, 203, 1505, 1601, 1719, 1895, 2147
Hpy8I GTNNAC 7 cut(s) 159, 203, 1505, 1601, 1719, 1895, 2147
HpyCH4III ACNGT 6 cut(s) 71, 317, 746, 801, 1707, 2144
HpyF10VI GCNNNNNNNGC 9 cut(s) 101, 110, 1162, 1461, 1658, 1878, 1980, 2418, 2801
HspAI GCGC 2 cut(s) 296, 355
Kpn2I TCCGGA 2 cut(s) 265, 1585
KpnI GGTACC 1 cut(s) 330
KspAI GTTAAC 1 cut(s) 1719
KspI CCGCGG 1 cut(s) 33
Kzo9I GATC 9 cut(s) 127, 141, 990, 1021, 1318, 1672, 2229, 2253, 3091
LguI GCTCTTC 1 cut(s) 102
LmnI GCTCC 5 cut(s) 268, 398, 476, 632, 2890
Lsp1109I GCAGC 8 cut(s) 26, 65, 68, 469, 1694, 1881, 2558, 2655
LweI GCATC 3 cut(s) 1400, 2708, 3034
MaeI CTAG 8 cut(s) 36, 1166, 1203, 1790, 1950, 2030, 2777, 2916
MalI GATC 9 cut(s) 129, 143, 992, 1023, 1320, 1674, 2231, 2255, 3093
MboI GATC 9 cut(s) 127, 141, 990, 1021, 1318, 1672, 2229, 2253, 3091
MfeI CAATTG 1 cut(s) 2871
MflI RGATCY 4 cut(s) 1021, 1318, 2229, 3091
MhlI GDGCHC 4 cut(s) 205, 257, 1212, 1328
MlsI TGGCCA 2 cut(s) 1176, 2462
MluNI TGGCCA 2 cut(s) 1176, 2462
MlyI GAGTC 5 cut(s) 98, 240, 1900, 2142, 2842
MmeI TCCRAC 5 cut(s) 783, 1538, 1806, 2117, 2658
Mox20I TGGCCA 2 cut(s) 1176, 2462
MroI TCCGGA 2 cut(s) 265, 1585
MroXI GAANNNNTTC 2 cut(s) 2347, 2440
MscI TGGCCA 2 cut(s) 1176, 2462
MslI CAYNNNNRTG 1 cut(s) 1614
Msp20I TGGCCA 2 cut(s) 1176, 2462
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 3 cut(s) 266, 1191, 1586
MspR9I CCNGG 3 cut(s) 26, 2226, 2627
MunI CAATTG 1 cut(s) 2871
Mva1269I GAATGC 1 cut(s) 792
MvaI CCWGG 3 cut(s) 26, 2226, 2627
MvnI CGCG 1 cut(s) 32
MwoI GCNNNNNNNGC 9 cut(s) 101, 110, 1162, 1461, 1658, 1878, 1980, 2418, 2801
NdeII GATC 9 cut(s) 127, 141, 990, 1021, 1318, 1672, 2229, 2253, 3091
NheI GCTAGC 1 cut(s) 35
NlaIV GGNNCC 9 cut(s) 328, 400, 421, 472, 634, 1048, 1651, 2624, 2886
NmuCI GTSAC 6 cut(s) 1338, 1415, 2519, 2540, 2750, 2911
NspI RCATGY 6 cut(s) 689, 712, 2527, 2614, 2668, 2801
PceI AGGCCT 1 cut(s) 1068
PciI ACATGT 2 cut(s) 708, 2797
PciSI GCTCTTC 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 355
PctI GAATGC 1 cut(s) 792
PdmI GAANNNNTTC 2 cut(s) 2347, 2440
PfeI GAWTC 6 cut(s) 381, 640, 1636, 2017, 2382, 2840
PflMI CCANNNNNTGG 3 cut(s) 153, 1549, 2265
PleI GAGTC 5 cut(s) 97, 240, 1899, 2142, 2842
PpsI GAGTC 5 cut(s) 97, 240, 1899, 2142, 2842
PpuMI RGGWCCY 1 cut(s) 420
PscI ACATGT 2 cut(s) 708, 2797
PshBI ATTAAT 1 cut(s) 960
PsiI TTATAA 1 cut(s) 2430
Psp124BI GAGCTC 1 cut(s) 1212
Psp5II RGGWCCY 1 cut(s) 420
Psp6I CCWGG 3 cut(s) 24, 2224, 2625
PspEI GGTNACC 1 cut(s) 275
PspFI CCCAGC 1 cut(s) 2762
PspGI CCWGG 3 cut(s) 24, 2224, 2625
PspN4I GGNNCC 9 cut(s) 328, 400, 421, 472, 634, 1048, 1651, 2624, 2886
PspPI GGNCC 6 cut(s) 420, 830, 1505, 1796, 1940, 2102
PspPPI RGGWCCY 1 cut(s) 420
PstI CTGCAG 1 cut(s) 2673
PstNI CAGNNNCTG 1 cut(s) 2571
PsuI RGATCY 4 cut(s) 1021, 1318, 2229, 3091
RsaI GTAC 5 cut(s) 328, 1125, 1980, 2339, 2894
RsaNI GTAC 5 cut(s) 327, 1124, 1979, 2338, 2893
RseI CAYNNNNRTG 1 cut(s) 1614
SacI GAGCTC 1 cut(s) 1212
SacII CCGCGG 1 cut(s) 33
SapI GCTCTTC 1 cut(s) 102
Sau3AI GATC 9 cut(s) 127, 141, 990, 1021, 1318, 1672, 2229, 2253, 3091
Sau96I GGNCC 6 cut(s) 420, 830, 1505, 1796, 1940, 2102
ScaI AGTACT 1 cut(s) 1125
SchI GAGTC 5 cut(s) 98, 240, 1900, 2142, 2842
ScrFI CCNGG 3 cut(s) 26, 2226, 2627
SduI GDGCHC 4 cut(s) 205, 257, 1212, 1328
SfaNI GCATC 3 cut(s) 1400, 2708, 3034
SfcI CTRYAG 4 cut(s) 81, 736, 2669, 3195
Sfr303I CCGCGG 1 cut(s) 33
SgrBI CCGCGG 1 cut(s) 33
SinI GGWCC 4 cut(s) 420, 830, 1505, 2102
SmiMI CAYNNNNRTG 1 cut(s) 1614
SmlI CTYRAG 3 cut(s) 304, 1459, 2048
SmoI CTYRAG 3 cut(s) 304, 1459, 2048
SseBI AGGCCT 1 cut(s) 1068
SsiI CCGC 5 cut(s) 30, 32, 1487, 2100, 2832
SspI AATATT 3 cut(s) 847, 1000, 2536
SspMI CTAG 8 cut(s) 36, 1166, 1203, 1790, 1950, 2030, 2777, 2916
SstI GAGCTC 1 cut(s) 1212
StuI AGGCCT 1 cut(s) 1068
StyD4I CCNGG 3 cut(s) 24, 2224, 2625
StyI CCWWGG 4 cut(s) 880, 1069, 1917, 2080
TaaI ACNGT 6 cut(s) 71, 317, 746, 801, 1707, 2144
TaqI TCGA 4 cut(s) 444, 460, 989, 1836
TaqII GACCGA 1 cut(s) 3236
TatI WGTACW 2 cut(s) 1123, 2337
TauI GCSGC 2 cut(s) 32, 35
TfiI GAWTC 6 cut(s) 381, 640, 1636, 2017, 2382, 2840
TscAI CASTG 4 cut(s) 130, 976, 1123, 2413
TseFI GTSAC 6 cut(s) 1338, 1415, 2519, 2540, 2750, 2911
TseI GCWGC 8 cut(s) 39, 53, 56, 482, 1682, 1869, 2571, 2668
Tsp45I GTSAC 6 cut(s) 1338, 1415, 2519, 2540, 2750, 2911
TspDTI ATGAA 8 cut(s) 311, 1087, 1393, 1605, 1767, 2159, 2340, 2708
TspGWI ACGGA 2 cut(s) 2910, 2915
TspRI CASTG 4 cut(s) 130, 976, 1123, 2413
Van91I CCANNNNNTGG 3 cut(s) 153, 1549, 2265
VneI GTGCAC 1 cut(s) 201
VpaK11BI GGWCC 4 cut(s) 420, 830, 1505, 2102
VspI ATTAAT 1 cut(s) 960
XapI RAATTY 8 cut(s) 615, 1288, 1400, 1545, 1900, 2088, 3028, 3098
XbaI TCTAGA 1 cut(s) 2029
XceI RCATGY 6 cut(s) 689, 712, 2527, 2614, 2668, 2801
XcmI CCANNNNNNNNNTGG 2 cut(s) 1740, 2266
XmiI GTMKAC 1 cut(s) 1894
XmnI GAANNNNTTC 2 cut(s) 2347, 2440
XspI CTAG 8 cut(s) 36, 1166, 1203, 1790, 1950, 2030, 2777, 2916
ZrmI AGTACT 1 cut(s) 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.