MD10G1210000.v1.1

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000364
Physical Location & Seq
Forward (+)
30893267 .. 30895219
1953 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1210000.v1.1.491

Sequence Viewer

Length: 735 bp
ATGGAAGTGTTTAGCTTTGAGAAAAGGATTAAGAATTACAGCAACTACCAGAAGATACTGTTAGTGGGAGAAGGAGACTTCTCTTTTGCCGTTTGCTTGGCCAGAGCTTTTGGCTTCGCTGTCAACATGGTTGCCACTTCTCTCGACTCCAGAGAGTCGTTAATGGTGAATTATTCAAAAGCTATAAGCAATGTGAAGGAATTAGAGTCCAGGGGATGCATTGTATTGCATGAGGTAGATGTCCACAGCATGAGCAGGCACCCTTTCCTGATTAGTGTACGCTTTGATCGCATAATCTATAATTTTCCTCACGCCGGTTACTTGCATGGCTCTGAACGCAACATGTTCCAGATTTGGTTTCATCAGGATTTGGTCAGGGGATTCTTTGAGAATGCAAGACAAATGCTGACCAGGGTAGGAGAAATTCATGTGACACACAAGACAACATTTCCTTTTAGTGAATGGAAAATAGTGGAGTTAGCTTATATGGCTGGATTATATTTGGTTCATGAAGAACAATTCTCGATATGGGATTATCCAGGTTATGAAAATAAGAGAGGAGCTGGGATGTGTGATCAAACTTTTCCTATTGGAATGTGTAGCACCTTCAAATTTGCCGAGATGTTGTACCGTTCTACCACTTCTGGTTTCCACCTTGGCGCTACTTCTTCGGGTCCATGGTCTGGATATTCTGGAATTAAGGGTCCTCACATGCAACTGCCATTTTACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

245

Amino Acids

28.08

Weight (kDa)

7.18

Isoelectric Point (pI)

32.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 20 - 186 5.2e-52 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 258
AccB7I CCANNNNNTGG 1 cut(s) 683
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 2 cut(s) 423, 611
AfaI GTAC 2 cut(s) 279, 629
AfiI CCNNNNNNNGG 2 cut(s) 314, 683
AflIII ACRYGT 1 cut(s) 342
AgsI TTSAA 2 cut(s) 177, 610
AjnI CCWGG 3 cut(s) 209, 410, 538
AluBI AGCT 5 cut(s) 15, 107, 182, 482, 563
AluI AGCT 5 cut(s) 15, 107, 182, 482, 563
Alw26I GTCTC 1 cut(s) 69
AoxI GGCC 1 cut(s) 99
ApoI RAATTY 2 cut(s) 423, 611
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AspLEI GCGC 1 cut(s) 662
AspS9I GGNCC 2 cut(s) 674, 704
AsuHPI GGTGA 1 cut(s) 178
AvaII GGWCC 2 cut(s) 674, 704
BalI TGGCCA 1 cut(s) 101
BanI GGYRCC 1 cut(s) 258
BceAI ACGGC 1 cut(s) 74
BciT130I CCWGG 3 cut(s) 211, 412, 540
BclI TGATCA 1 cut(s) 574
BcoDI GTCTC 1 cut(s) 69
BfoI RGCGCY 1 cut(s) 663
Bme1390I CCNGG 3 cut(s) 211, 412, 540
Bme18I GGWCC 2 cut(s) 674, 704
BmgT120I GGNCC 2 cut(s) 674, 704
BmiI GGNNCC 3 cut(s) 260, 675, 705
BmrFI CCNGG 3 cut(s) 211, 412, 540
BmsI GCATC 1 cut(s) 206
BpmI CTGGAG 1 cut(s) 133
BsaJI CCNNGG 4 cut(s) 210, 411, 655, 677
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 683
Bse118I RCCGGY 1 cut(s) 314
Bse3DI GCAATG 1 cut(s) 196
BseBI CCWGG 3 cut(s) 211, 412, 540
BseDI CCNNGG 4 cut(s) 210, 411, 655, 677
BseGI GGATG 2 cut(s) 221, 573
BseLI CCNNNNNNNGG 2 cut(s) 314, 683
BseMI GCAATG 1 cut(s) 196
BseRI GAGGAG 1 cut(s) 573
BseYI CCCAGC 1 cut(s) 563
BshFI GGCC 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 258
BsiSI CCGG 1 cut(s) 315
BslI CCNNNNNNNGG 2 cut(s) 314, 683
BsmAI GTCTC 1 cut(s) 69
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 1 cut(s) 101
Bsp143I GATC 2 cut(s) 286, 574
Bsp19I CCATGG 1 cut(s) 677
BspANI GGCC 1 cut(s) 101
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 3 cut(s) 260, 675, 705
BspT107I GGYRCC 1 cut(s) 258
BsrDI GCAATG 1 cut(s) 196
BsrFI RCCGGY 1 cut(s) 314
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 4 cut(s) 210, 411, 655, 677
BssMI GATC 2 cut(s) 286, 574
BssT1I CCWWGG 2 cut(s) 655, 677
Bst2UI CCWGG 3 cut(s) 211, 412, 540
Bst4CI ACNGT 2 cut(s) 60, 632
BstC8I GCNNGC 1 cut(s) 257
BstDSI CCRYGG 1 cut(s) 677
BstF5I GGATG 2 cut(s) 221, 573
BstH2I RGCGCY 1 cut(s) 663
BstHHI GCGC 1 cut(s) 662
BstKTI GATC 2 cut(s) 289, 577
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 2 cut(s) 286, 574
BstMWI GCNNNNNNNGC 3 cut(s) 288, 336, 488
BstNI CCWGG 3 cut(s) 211, 412, 540
BstNSI RCATGY 2 cut(s) 346, 715
BstSCI CCNGG 3 cut(s) 209, 410, 538
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 1 cut(s) 677
BtsCI GGATG 2 cut(s) 221, 573
Cac8I GCNNGC 1 cut(s) 257
CciI TCATGA 1 cut(s) 508
CfoI GCGC 1 cut(s) 662
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 2 cut(s) 674, 704
Csp6I GTAC 2 cut(s) 278, 628
CviAII CATG 9 cut(s) 127, 230, 250, 326, 343, 428, 509, 678, 712
CviJI RGCY 9 cut(s) 15, 101, 107, 114, 182, 330, 482, 491, 563
CviKI_1 RGCY 9 cut(s) 15, 101, 107, 114, 182, 330, 482, 491, 563
CviQI GTAC 2 cut(s) 278, 628
DpnI GATC 2 cut(s) 288, 576
DpnII GATC 2 cut(s) 286, 574
EaeI YGGCCR 1 cut(s) 99
Eco130I CCWWGG 2 cut(s) 655, 677
Eco47I GGWCC 2 cut(s) 674, 704
EcoO109I RGGNCCY 1 cut(s) 704
EcoRII CCWGG 3 cut(s) 209, 410, 538
EcoT14I CCWWGG 2 cut(s) 655, 677
EcoT22I ATGCAT 1 cut(s) 221
ErhI CCWWGG 2 cut(s) 655, 677
FaeI CATG 9 cut(s) 130, 233, 253, 329, 346, 431, 512, 681, 715
FatI CATG 9 cut(s) 126, 229, 249, 325, 342, 427, 508, 677, 711
FbaI TGATCA 1 cut(s) 574
FokI GGATG 2 cut(s) 228, 580
GlaI GCGC 1 cut(s) 661
GsaI CCCAGC 1 cut(s) 567
GsuI CTGGAG 1 cut(s) 133
HaeII RGCGCY 1 cut(s) 663
HaeIII GGCC 1 cut(s) 101
HapII CCGG 1 cut(s) 315
HhaI GCGC 1 cut(s) 662
Hin1II CATG 9 cut(s) 130, 233, 253, 329, 346, 431, 512, 681, 715
Hin6I GCGC 1 cut(s) 660
HinP1I GCGC 1 cut(s) 660
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 4 cut(s) 146, 155, 206, 381
HpaII CCGG 1 cut(s) 315
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 3 cut(s) 124, 244, 278
Hpy188I TCNGA 1 cut(s) 334
Hpy188III TCNNGA 9 cut(s) 143, 150, 268, 349, 365, 509, 523, 684, 693
Hpy8I GTNNAC 3 cut(s) 124, 244, 278
HpyAV CCTTC 3 cut(s) 65, 190, 616
HpyCH4III ACNGT 2 cut(s) 60, 632
HpyCH4V TGCA 5 cut(s) 219, 229, 325, 395, 715
HpyF10VI GCNNNNNNNGC 3 cut(s) 288, 336, 488
Hsp92II CATG 9 cut(s) 130, 233, 253, 329, 346, 431, 512, 681, 715
HspAI GCGC 1 cut(s) 660
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 2 cut(s) 286, 574
LmnI GCTCC 1 cut(s) 560
LweI GCATC 1 cut(s) 206
MaeIII GTNAC 2 cut(s) 317, 430
MalI GATC 2 cut(s) 288, 576
MboI GATC 2 cut(s) 286, 574
MboII GAAGA 3 cut(s) 64, 524, 660
MlsI TGGCCA 1 cut(s) 101
MluCI AATT 8 cut(s) 34, 169, 200, 301, 423, 518, 611, 696
MluNI TGGCCA 1 cut(s) 101
MlyI GAGTC 3 cut(s) 140, 164, 215
MnlI CCTC 4 cut(s) 226, 318, 551, 717
Mox20I TGGCCA 1 cut(s) 101
Mph1103I ATGCAT 1 cut(s) 221
MscI TGGCCA 1 cut(s) 101
MseI TTAA 3 cut(s) 30, 161, 699
Msp20I TGGCCA 1 cut(s) 101
MspI CCGG 1 cut(s) 315
MspR9I CCNGG 3 cut(s) 211, 412, 540
Mva1269I GAATGC 1 cut(s) 397
MvaI CCWGG 3 cut(s) 211, 412, 540
MwoI GCNNNNNNNGC 3 cut(s) 288, 336, 488
NcoI CCATGG 1 cut(s) 677
NdeII GATC 2 cut(s) 286, 574
NlaIII CATG 9 cut(s) 130, 233, 253, 329, 346, 431, 512, 681, 715
NlaIV GGNNCC 3 cut(s) 260, 675, 705
NmeAIII GCCGAG 1 cut(s) 643
NmuCI GTSAC 1 cut(s) 430
NsiI ATGCAT 1 cut(s) 221
NspI RCATGY 2 cut(s) 346, 715
PagI TCATGA 1 cut(s) 508
PciI ACATGT 1 cut(s) 342
PctI GAATGC 1 cut(s) 397
PfeI GAWTC 1 cut(s) 381
PflMI CCANNNNNTGG 1 cut(s) 683
PleI GAGTC 3 cut(s) 140, 163, 214
PpsI GAGTC 3 cut(s) 140, 163, 214
PpuMI RGGWCCY 1 cut(s) 704
PscI ACATGT 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 704
Psp6I CCWGG 3 cut(s) 209, 410, 538
PspFI CCCAGC 1 cut(s) 563
PspGI CCWGG 3 cut(s) 209, 410, 538
PspN4I GGNNCC 3 cut(s) 260, 675, 705
PspPI GGNCC 2 cut(s) 674, 704
PspPPI RGGWCCY 1 cut(s) 704
RsaI GTAC 2 cut(s) 279, 629
RsaNI GTAC 2 cut(s) 278, 628
SaqAI TTAA 3 cut(s) 30, 161, 699
Sau3AI GATC 2 cut(s) 286, 574
Sau96I GGNCC 2 cut(s) 674, 704
SchI GAGTC 3 cut(s) 140, 164, 215
ScrFI CCNGG 3 cut(s) 211, 412, 540
SetI ASST 9 cut(s) 17, 109, 184, 237, 484, 544, 565, 608, 657
SfaNI GCATC 1 cut(s) 206
SinI GGWCC 2 cut(s) 674, 704
Sse9I AATT 8 cut(s) 34, 169, 200, 301, 423, 518, 611, 696
StyD4I CCNGG 3 cut(s) 209, 410, 538
StyI CCWWGG 2 cut(s) 655, 677
TaaI ACNGT 2 cut(s) 60, 632
TaqI TCGA 2 cut(s) 144, 524
TasI AATT 8 cut(s) 34, 169, 200, 301, 423, 518, 611, 696
TfiI GAWTC 1 cut(s) 381
Tru1I TTAA 3 cut(s) 30, 161, 699
Tru9I TTAA 3 cut(s) 30, 161, 699
TseFI GTSAC 1 cut(s) 430
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 5 cut(s) 350, 416, 497, 525, 561
Van91I CCANNNNNTGG 1 cut(s) 683
VpaK11BI GGWCC 2 cut(s) 674, 704
XapI RAATTY 2 cut(s) 423, 611
XceI RCATGY 2 cut(s) 346, 715
Zsp2I ATGCAT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.