Rw5G015750

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0018612
Physical Location & Seq
Reverse (-)
19584460 .. 19585182
723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G015750.1

Sequence Viewer

Length: 588 bp
ATGGGTTTTGCTACAGAACGCGAAAAATTGGTCTACACTGCTAAGCTGGCTCAGGAGGCAGAGCGATACGATGAGATGACGGAGGAGGCGAAAGGAAATAATATTCACATGAAGCATCTAAAGAAATATAGACAGAGAGTTGAATCAGAGATATCAATTATTTGTAGAGATATCATCTCAGTGCTTGATAGTCATCTCATCCCTTCGACTTCAGATGGTGAATCAACTGTGCATTATCATAAGATGAAAGGAGACTTTTATAGGTATCTCGCAGAAATCAAGGCCGGTGATGAGAAAGAGGAGGCTATTAAACAGTCGAAGAAAGCATATTTCAAGGCCCTCACTGTTGCAGAAGCTGAGTTGCCTCCTACAAATCCCATCCGTTTGGGTGCGGCTTTGCACTTGTCAATCTTATATCATGAGTTCCTGAATAATCCGGAGACGGCCTGCCGTCTTACAAAGATAGCTTTTGAGGAAGGCACTGCTGAGATTAACACACTGAACAATGAACCATACAAGAATACCCTCATGGCCTTGAAACTTTTAAGGGAAAACTACAGCTTGTGGACTTCCCAACCTCCACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

195

Amino Acids

22.39

Weight (kDa)

6.26

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 28 - 190 7.5e-56 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018612)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0024421 RchiOBHm_Chr5g0024441
rosa_laevigata RLG00000032798
rosa_multiflora Rmu_sc0005861.1_g000014
rosa_rugosa Rorug05G0083400
rosa_samantha Rh5AG174400 Rh5BG172200 Rh5CG188100 Rh5DG173600
rosa_wichuraiana Rw5G015750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 33
AccII CGCG 1 cut(s) 21
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 1 cut(s) 392
AcuI CTGAAG 1 cut(s) 195
AgsI TTSAA 3 cut(s) 143, 334, 538
AluBI AGCT 4 cut(s) 46, 356, 467, 561
AluI AGCT 4 cut(s) 46, 356, 467, 561
Alw26I GTCTC 2 cut(s) 246, 434
AlwNI CAGNNNCTG 1 cut(s) 356
Aor13HI TCCGGA 1 cut(s) 436
AoxI GGCC 4 cut(s) 282, 336, 444, 531
ArsI GACNNNNNNTTYG 2 cut(s) 15, 47
AspS9I GGNCC 1 cut(s) 337
AsuHPI GGTGA 2 cut(s) 230, 299
BccI CCATC 2 cut(s) 209, 386
BceAI ACGGC 2 cut(s) 435, 459
BcoDI GTCTC 2 cut(s) 246, 434
BfmI CTRYAG 2 cut(s) 12, 556
BisI GCNGC 1 cut(s) 393
BlpI GCTNAGC 1 cut(s) 42
BlsI GCNGC 1 cut(s) 394
BmgT120I GGNCC 1 cut(s) 337
BmsI GCATC 1 cut(s) 124
Bpu10I CCTNAGC 1 cut(s) 51
Bpu1102I GCTNAGC 1 cut(s) 42
BsaBI GATNNNNATC 1 cut(s) 192
BsaWI WCCGGW 1 cut(s) 436
Bse118I RCCGGY 1 cut(s) 284
Bse8I GATNNNNATC 1 cut(s) 192
BseAI TCCGGA 1 cut(s) 436
BseGI GGATG 2 cut(s) 198, 378
BseJI GATNNNNATC 1 cut(s) 192
BseMII CTCAG 4 cut(s) 65, 192, 348, 477
BseRI GAGGAG 2 cut(s) 98, 314
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 4 cut(s) 284, 338, 446, 533
BsiSI CCGG 2 cut(s) 285, 437
BsmAI GTCTC 2 cut(s) 246, 434
BsmBI CGTCTC 1 cut(s) 434
BsnI GGCC 4 cut(s) 284, 338, 446, 533
Bsp13I TCCGGA 1 cut(s) 436
Bsp1720I GCTNAGC 1 cut(s) 42
BspACI CCGC 1 cut(s) 392
BspANI GGCC 4 cut(s) 284, 338, 446, 533
BspCNI CTCAG 4 cut(s) 64, 191, 349, 478
BspEI TCCGGA 1 cut(s) 436
BspFNI CGCG 1 cut(s) 21
BspHI TCATGA 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 284
BssAI RCCGGY 1 cut(s) 284
Bst4CI ACNGT 3 cut(s) 229, 315, 346
BstC8I GCNNGC 2 cut(s) 48, 448
BstDEI CTNAG 5 cut(s) 42, 51, 178, 357, 486
BstF5I GGATG 2 cut(s) 198, 378
BstFNI CGCG 1 cut(s) 21
BstMAI GTCTC 2 cut(s) 246, 434
BstMWI GCNNNNNNNGC 2 cut(s) 47, 56
BstSFI CTRYAG 2 cut(s) 12, 556
BstUI CGCG 1 cut(s) 21
BstXI CCANNNNNNTGG 1 cut(s) 385
BsuRI GGCC 4 cut(s) 284, 338, 446, 533
BtsCI GGATG 2 cut(s) 198, 378
BtsI GCAGTG 2 cut(s) 36, 480
BtsIMutI CAGTG 5 cut(s) 36, 186, 342, 480, 497
Cac8I GCNNGC 2 cut(s) 48, 448
CaiI CAGNNNCTG 1 cut(s) 356
CciI TCATGA 1 cut(s) 418
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 1 cut(s) 337
CviAII CATG 3 cut(s) 109, 419, 529
DdeI CTNAG 5 cut(s) 42, 51, 178, 357, 486
Eco32I GATATC 2 cut(s) 153, 172
Eco57I CTGAAG 1 cut(s) 195
EcoO109I RGGNCCY 1 cut(s) 337
EcoRV GATATC 2 cut(s) 153, 172
Esp3I CGTCTC 1 cut(s) 434
FaeI CATG 3 cut(s) 112, 422, 532
FaiI YATR 9 cut(s) 110, 129, 240, 261, 328, 415, 420, 514, 530
FatI CATG 3 cut(s) 108, 418, 528
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 1 cut(s) 393
FokI GGATG 2 cut(s) 185, 365
Fsp4HI GCNGC 1 cut(s) 393
GluI GCNGC 1 cut(s) 393
HaeIII GGCC 4 cut(s) 284, 338, 446, 533
HapII CCGG 2 cut(s) 285, 437
Hin1II CATG 3 cut(s) 112, 422, 532
HinfI GANTC 2 cut(s) 143, 221
HpaII CCGG 2 cut(s) 285, 437
HphI GGTGA 2 cut(s) 230, 299
Hpy166II GTNNAC 2 cut(s) 34, 567
Hpy188I TCNGA 2 cut(s) 148, 214
Hpy188III TCNNGA 4 cut(s) 53, 419, 427, 437
Hpy8I GTNNAC 2 cut(s) 34, 567
HpyAV CCTTC 2 cut(s) 213, 470
HpyCH4III ACNGT 3 cut(s) 229, 315, 346
HpyCH4V TGCA 3 cut(s) 232, 350, 400
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 56
HpyF3I CTNAG 5 cut(s) 42, 51, 178, 357, 486
Hsp92II CATG 3 cut(s) 112, 422, 532
Kpn2I TCCGGA 1 cut(s) 436
LpnPI CCDG 6 cut(s) 32, 38, 298, 440, 450, 460
LweI GCATC 1 cut(s) 124
MboII GAAGA 1 cut(s) 331
MluCI AATT 2 cut(s) 26, 156
MroI TCCGGA 1 cut(s) 436
MseI TTAA 4 cut(s) 309, 492, 545, 586
MslI CAYNNNNRTG 1 cut(s) 179
MspI CCGG 2 cut(s) 285, 437
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 2 cut(s) 47, 56
NlaIII CATG 3 cut(s) 112, 422, 532
PagI TCATGA 1 cut(s) 418
PcsI WCGNNNNNNNCGW 1 cut(s) 86
PfeI GAWTC 2 cut(s) 143, 221
PkrI GCNGC 1 cut(s) 394
PspPI GGNCC 1 cut(s) 337
PstNI CAGNNNCTG 1 cut(s) 356
RseI CAYNNNNRTG 1 cut(s) 179
SaqAI TTAA 4 cut(s) 309, 492, 545, 586
SatI GCNGC 1 cut(s) 393
Sau96I GGNCC 1 cut(s) 337
SetI ASST 6 cut(s) 48, 266, 358, 469, 563, 580
SfaNI GCATC 1 cut(s) 124
SfcI CTRYAG 2 cut(s) 12, 556
SmiMI CAYNNNNRTG 1 cut(s) 179
Sse9I AATT 2 cut(s) 26, 156
SsiI CCGC 1 cut(s) 392
SspI AATATT 1 cut(s) 103
TaaI ACNGT 3 cut(s) 229, 315, 346
TaqI TCGA 2 cut(s) 206, 317
TasI AATT 2 cut(s) 26, 156
TauI GCSGC 1 cut(s) 395
TfiI GAWTC 2 cut(s) 143, 221
Tru1I TTAA 4 cut(s) 309, 492, 545, 586
Tru9I TTAA 4 cut(s) 309, 492, 545, 586
TscAI CASTG 5 cut(s) 43, 186, 349, 487, 504
TspDTI ATGAA 3 cut(s) 125, 260, 522
TspGWI ACGGA 2 cut(s) 95, 371
TspRI CASTG 5 cut(s) 43, 186, 349, 487, 504
XmiI GTMKAC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.