RchiOBHm_Chr5g0030821

poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0019421
Physical Location & Seq
Reverse (-)
24495791 .. 24496852
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31013

Sequence Viewer

Length: 1062 bp
ATGGACCAGACCCAGATTGTTGAAAATGAAGCTTCCGGTTCCAATGTGGATATAACGGAGGTCGAGAAGCATCAAGATTCCGTTTCCGGTGCCGGTGCCGCCGAAGCTGATCCCAAGGCTGACAAGAACGCTAACGAATCCGGGTCGGGTCCTTCCGGATCCGGTTCCTCTGATCCAGACCCGTTTAGGGTTTTCACCCAGGACGGAACGATGTTGAGAGTAAGGGGAGCCAGTGCGGATCTAATAATAGTCAAGAGGAACTTCCTTTCCGGTATGGCCTTGGCCGCCGGAGAAACCACAGTGGTGGCCGTGCACAAGTATCCGGCTTCTAATCTGACCCATCGGGCCCGGTTCCAGGCTTTTCGGGGTTTCTTGGCGGAGACGGTTCATAAATATGGTGGAATTGTCCAAAATGCCCATCTCAAGACTGCGCTGTACGGCGGCTCTAGGGACGAGATTGCTCAGATTTTGGTTCATGGGTTTAGTGCTGCCAATGCTAATGGTGGCGCCCATGGTTGTGTTCAATTGGTTCCTGCTGCGCATTCTTTGGATGCTGCATTGTTCTGTGAAGTTGATGAAGCTGAGCTAAGGCACGTACTGGTGTGCCGCGTCATAATGGGGAGGGTGCAATTCGTCCCTGATGGCTCGAACCATGTTGGTCCCGATTTCGATACCGGTTGTGACAGTTTTACCGATGCTAGGACGCTTTATGTGCCTGCCGGTTCAGCCAACTCTCGCATTTGTGCTGATTTTGTTATAAGCTTTAGGGCTCCTTCTTGCTTATGGGATTATGCAACATATGCTACTCAGGCGAGTGGTGGTTTTGTTGGTGCTTCTTCAAGGGGGCCTAGACCACAAGAACTACCCATTAATTTGTTTGCTGTGTTGGACCGGCTCGCAAGTTTTGTCACTCCTCTACAAATGACCATTGCTGTCGAGTTGTTTTGGAAGTATAAGGGAAACAACATGGGCCGACATGAGCTGATTCGACAACTGAGACTCATGGTTGGTGAGGAGGCGTTGGTTGCAGCGGCCAAGGCATCAGCTTCGGTGAACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PARP RST

Protein Analysis

353

Amino Acids

37.54

Weight (kDa)

5.88

Isoelectric Point (pI)

25.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 285 - 346 9e-07 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019421)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0030821
rosa_laevigata RLG00000033257
rosa_multiflora Rmu_sc0001974.1_g000030
rosa_roxburghii Rroxscaffold_1G00049420
rosa_rugosa Rorug05G0121000
rosa_samantha Rh5BG212900 Rh5CG236200 Rh5DG217400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 758
Acc16I TGCGCA 1 cut(s) 540
AccB1I GGYRCC 3 cut(s) 89, 95, 506
AccII CGCG 1 cut(s) 609
AccIII TCCGGA 1 cut(s) 155
AciI CCGC 7 cut(s) 99, 236, 285, 377, 441, 607, 1031
AclWI GGATC 5 cut(s) 104, 153, 166, 167, 246
AcoI YGGCCR 3 cut(s) 282, 306, 1032
AcyI GRCGYC 1 cut(s) 507
AfaI GTAC 2 cut(s) 437, 597
AfiI CCNNNNNNNGG 3 cut(s) 187, 355, 699
AgeI ACCGGT 1 cut(s) 674
AgsI TTSAA 3 cut(s) 23, 524, 840
AjnI CCWGG 2 cut(s) 198, 354
AleI CACNNNNGTG 1 cut(s) 302
AloI GAACNNNNNNTCC 2 cut(s) 251, 283
AluBI AGCT 7 cut(s) 32, 107, 581, 586, 762, 982, 1046
AluI AGCT 7 cut(s) 32, 107, 581, 586, 762, 982, 1046
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 374, 991
Alw44I GTGCAC 1 cut(s) 311
AlwI GGATC 5 cut(s) 104, 153, 166, 167, 246
Aor13HI TCCGGA 1 cut(s) 155
AoxI GGCC 7 cut(s) 276, 282, 306, 345, 845, 970, 1032
ApaI GGGCCC 1 cut(s) 349
ApaLI GTGCAC 1 cut(s) 311
ApeKI GCWGC 4 cut(s) 488, 536, 554, 1028
AseI ATTAAT 1 cut(s) 870
AsiGI ACCGGT 1 cut(s) 674
AspLEI GCGC 3 cut(s) 433, 509, 541
AspS9I GGNCC 8 cut(s) 4, 149, 345, 346, 659, 845, 889, 970
AsuC2I CCSGG 2 cut(s) 142, 349
AsuHPI GGTGA 2 cut(s) 187, 1022
AvaII GGWCC 4 cut(s) 4, 149, 659, 889
BaeGI GKGCMC 2 cut(s) 315, 349
BamHI GGATCC 1 cut(s) 158
BanI GGYRCC 3 cut(s) 89, 95, 506
BanII GRGCYC 2 cut(s) 349, 772
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 4 cut(s) 475, 523, 541, 1040
BccI CCATC 3 cut(s) 348, 426, 635
BceAI ACGGC 2 cut(s) 293, 454
BcgI CGANNNNNNTGC 1 cut(s) 1029
BciT130I CCWGG 2 cut(s) 200, 356
BciVI GTATCC 1 cut(s) 330
BcnI CCSGG 2 cut(s) 142, 349
BcoDI GTCTC 2 cut(s) 374, 991
BfaI CTAG 3 cut(s) 447, 699, 849
BfoI RGCGCY 1 cut(s) 510
BfuI GTATCC 1 cut(s) 330
BisI GCNGC 9 cut(s) 99, 285, 442, 489, 537, 555, 607, 1029, 1032
BlpI GCTNAGC 1 cut(s) 582
BlsI GCNGC 9 cut(s) 100, 286, 443, 490, 538, 556, 608, 1030, 1033
Bme1390I CCNGG 4 cut(s) 142, 200, 349, 356
Bme18I GGWCC 4 cut(s) 4, 149, 659, 889
BmgT120I GGNCC 8 cut(s) 4, 149, 345, 346, 659, 845, 889, 970
BmrFI CCNGG 4 cut(s) 142, 200, 349, 356
BmsI GCATC 4 cut(s) 79, 541, 685, 1049
Bpu10I CCTNAGC 1 cut(s) 587
Bpu1102I GCTNAGC 1 cut(s) 582
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 2 cut(s) 142, 349
BsaAI YACGTR 1 cut(s) 595
BsaHI GRCGYC 1 cut(s) 507
BsaJI CCNNGG 5 cut(s) 114, 198, 279, 511, 1035
BsaWI WCCGGW 6 cut(s) 35, 86, 155, 161, 269, 674
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 355, 699
Bse118I RCCGGY 4 cut(s) 92, 674, 719, 891
Bse1I ACTGG 2 cut(s) 231, 603
Bse3DI GCAATG 1 cut(s) 927
BseAI TCCGGA 1 cut(s) 155
BseBI CCWGG 2 cut(s) 200, 356
BseDI CCNNGG 5 cut(s) 114, 198, 279, 511, 1035
BseGI GGATG 1 cut(s) 556
BseLI CCNNNNNNNGG 3 cut(s) 187, 355, 699
BseMI GCAATG 1 cut(s) 927
BseMII CTCAG 4 cut(s) 476, 573, 821, 986
BseNI ACTGG 2 cut(s) 231, 603
BseRI GAGGAG 2 cut(s) 903, 1028
BseSI GKGCMC 2 cut(s) 315, 349
BseXI GCAGC 4 cut(s) 475, 523, 541, 1040
Bsh1236I CGCG 1 cut(s) 609
BshFI GGCC 7 cut(s) 278, 284, 308, 347, 847, 972, 1034
BshNI GGYRCC 3 cut(s) 89, 95, 506
BshTI ACCGGT 1 cut(s) 674
BsiHKAI GWGCWC 1 cut(s) 315
BslFI GGGAC 3 cut(s) 464, 620, 645
BslI CCNNNNNNNGG 3 cut(s) 187, 355, 699
BsmAI GTCTC 2 cut(s) 374, 991
BsmBI CGTCTC 1 cut(s) 374
BsmFI GGGAC 3 cut(s) 464, 620, 645
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 7 cut(s) 278, 284, 308, 347, 847, 972, 1034
Bsp120I GGGCCC 1 cut(s) 345
Bsp1286I GDGCHC 3 cut(s) 315, 349, 772
Bsp13I TCCGGA 1 cut(s) 155
Bsp143I GATC 4 cut(s) 109, 158, 172, 238
Bsp1720I GCTNAGC 1 cut(s) 582
Bsp19I CCATGG 1 cut(s) 511
BspACI CCGC 7 cut(s) 99, 236, 285, 377, 441, 607, 1031
BspANI GGCC 7 cut(s) 278, 284, 308, 347, 847, 972, 1034
BspCNI CTCAG 4 cut(s) 475, 574, 820, 987
BspEI TCCGGA 1 cut(s) 155
BspFNI CGCG 1 cut(s) 609
BspPI GGATC 5 cut(s) 104, 153, 166, 167, 246
BspT107I GGYRCC 3 cut(s) 89, 95, 506
BsrDI GCAATG 1 cut(s) 927
BsrFI RCCGGY 4 cut(s) 92, 674, 719, 891
BsrI ACTGG 2 cut(s) 231, 603
BssAI RCCGGY 4 cut(s) 92, 674, 719, 891
BssECI CCNNGG 5 cut(s) 114, 198, 279, 511, 1035
BssMI GATC 4 cut(s) 109, 158, 172, 238
BssNI GRCGYC 1 cut(s) 507
BssT1I CCWWGG 4 cut(s) 114, 279, 511, 1035
Bst2UI CCWGG 2 cut(s) 200, 356
Bst4CI ACNGT 3 cut(s) 301, 385, 686
BstACI GRCGYC 1 cut(s) 507
BstAPI GCANNNNNTGC 1 cut(s) 800
BstBAI YACGTR 1 cut(s) 595
BstC8I GCNNGC 2 cut(s) 717, 897
BstDEI CTNAG 5 cut(s) 462, 582, 587, 807, 995
BstDSI CCRYGG 1 cut(s) 511
BstF5I GGATG 1 cut(s) 556
BstFNI CGCG 1 cut(s) 609
BstH2I RGCGCY 1 cut(s) 510
BstHHI GCGC 3 cut(s) 433, 509, 541
BstKTI GATC 4 cut(s) 112, 161, 175, 241
BstMAI GTCTC 2 cut(s) 374, 991
BstMBI GATC 4 cut(s) 109, 158, 172, 238
BstNI CCWGG 2 cut(s) 200, 356
BstSCI CCNGG 4 cut(s) 140, 198, 347, 354
BstSLI GKGCMC 2 cut(s) 315, 349
BstUI CGCG 1 cut(s) 609
BstV1I GCAGC 4 cut(s) 475, 523, 541, 1040
BstX2I RGATCY 2 cut(s) 158, 238
BstXI CCANNNNNNTGG 1 cut(s) 304
BstYI RGATCY 2 cut(s) 158, 238
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 7 cut(s) 278, 284, 308, 347, 847, 972, 1034
BtgI CCRYGG 1 cut(s) 511
BtsCI GGATG 1 cut(s) 556
BtsIMutI CAGTG 2 cut(s) 238, 306
Cac8I GCNNGC 2 cut(s) 717, 897
CfoI GCGC 3 cut(s) 433, 509, 541
Cfr10I RCCGGY 4 cut(s) 92, 674, 719, 891
Cfr13I GGNCC 8 cut(s) 4, 149, 345, 346, 659, 845, 889, 970
CseI GACGC 2 cut(s) 598, 712
Csp6I GTAC 2 cut(s) 436, 596
CspAI ACCGGT 1 cut(s) 674
CviAII CATG 6 cut(s) 476, 512, 653, 967, 977, 1003
CviQI GTAC 2 cut(s) 436, 596
DdeI CTNAG 5 cut(s) 462, 582, 587, 807, 995
DinI GGCGCC 1 cut(s) 508
DpnI GATC 4 cut(s) 111, 160, 174, 240
DpnII GATC 4 cut(s) 109, 158, 172, 238
EaeI YGGCCR 3 cut(s) 282, 306, 1032
EciI GGCGGA 1 cut(s) 392
Eco130I CCWWGG 4 cut(s) 114, 279, 511, 1035
Eco24I GRGCYC 2 cut(s) 349, 772
Eco47I GGWCC 4 cut(s) 4, 149, 659, 889
EcoO109I RGGNCCY 2 cut(s) 149, 845
EcoRII CCWGG 2 cut(s) 198, 354
EcoT14I CCWWGG 4 cut(s) 114, 279, 511, 1035
EcoT38I GRGCYC 2 cut(s) 349, 772
EgeI GGCGCC 1 cut(s) 508
EheI GGCGCC 1 cut(s) 508
ErhI CCWWGG 4 cut(s) 114, 279, 511, 1035
Esp3I CGTCTC 1 cut(s) 374
FaeI CATG 6 cut(s) 479, 515, 656, 970, 980, 1006
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 3 cut(s) 464, 620, 645
FatI CATG 6 cut(s) 475, 511, 652, 966, 976, 1002
FauNDI CATATG 1 cut(s) 799
Fnu4HI GCNGC 9 cut(s) 99, 285, 442, 489, 537, 555, 607, 1029, 1032
FokI GGATG 1 cut(s) 563
FriOI GRGCYC 2 cut(s) 349, 772
Fsp4HI GCNGC 9 cut(s) 99, 285, 442, 489, 537, 555, 607, 1029, 1032
FspBI CTAG 3 cut(s) 447, 699, 849
FspI TGCGCA 1 cut(s) 540
GlaI GCGC 3 cut(s) 432, 508, 540
GluI GCNGC 9 cut(s) 99, 285, 442, 489, 537, 555, 607, 1029, 1032
HaeII RGCGCY 1 cut(s) 510
HaeIII GGCC 7 cut(s) 278, 284, 308, 347, 847, 972, 1034
HgaI GACGC 2 cut(s) 598, 712
HhaI GCGC 3 cut(s) 433, 509, 541
Hin1I GRCGYC 1 cut(s) 507
Hin1II CATG 6 cut(s) 479, 515, 656, 970, 980, 1006
Hin6I GCGC 3 cut(s) 431, 507, 539
HinP1I GCGC 3 cut(s) 431, 507, 539
HindIII AAGCTT 2 cut(s) 30, 760
HinfI GANTC 4 cut(s) 77, 137, 985, 999
HphI GGTGA 2 cut(s) 187, 1022
Hpy166II GTNNAC 2 cut(s) 313, 1054
Hpy188I TCNGA 3 cut(s) 172, 336, 465
Hpy188III TCNNGA 7 cut(s) 64, 74, 156, 176, 253, 424, 662
Hpy8I GTNNAC 2 cut(s) 313, 1054
HpyAV CCTTC 2 cut(s) 162, 783
HpyCH4III ACNGT 3 cut(s) 301, 385, 686
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 5 cut(s) 313, 557, 628, 794, 1028
HpyF3I CTNAG 5 cut(s) 462, 582, 587, 807, 995
HpySE526I ACGT 1 cut(s) 594
Hsp92I GRCGYC 1 cut(s) 507
Hsp92II CATG 6 cut(s) 479, 515, 656, 970, 980, 1006
HspAI GCGC 3 cut(s) 431, 507, 539
KasI GGCGCC 1 cut(s) 506
Kpn2I TCCGGA 1 cut(s) 155
Kzo9I GATC 4 cut(s) 109, 158, 172, 238
LmnI GCTCC 2 cut(s) 227, 775
Lsp1109I GCAGC 4 cut(s) 475, 523, 541, 1040
LweI GCATC 4 cut(s) 79, 541, 685, 1049
MaeI CTAG 3 cut(s) 447, 699, 849
MaeII ACGT 1 cut(s) 594
MaeIII GTNAC 2 cut(s) 680, 907
MalI GATC 4 cut(s) 111, 160, 174, 240
MboI GATC 4 cut(s) 109, 158, 172, 238
MboII GAAGA 1 cut(s) 828
MfeI CAATTG 1 cut(s) 524
MflI RGATCY 2 cut(s) 158, 238
MhlI GDGCHC 3 cut(s) 315, 349, 772
MluCI AATT 4 cut(s) 402, 524, 629, 871
Mly113I GGCGCC 1 cut(s) 507
MlyI GAGTC 1 cut(s) 993
MmeI TCCRAC 1 cut(s) 867
MnlI CCTC 7 cut(s) 52, 178, 249, 615, 924, 1006, 1009
MroI TCCGGA 1 cut(s) 155
MseI TTAA 1 cut(s) 870
MslI CAYNNNNRTG 3 cut(s) 302, 393, 516
MspA1I CMGCKG 1 cut(s) 1031
MspR9I CCNGG 4 cut(s) 142, 200, 349, 356
MunI CAATTG 1 cut(s) 524
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 2 cut(s) 200, 356
MvnI CGCG 1 cut(s) 609
NarI GGCGCC 1 cut(s) 507
NciI CCSGG 2 cut(s) 142, 349
NcoI CCATGG 1 cut(s) 511
NdeI CATATG 1 cut(s) 799
NdeII GATC 4 cut(s) 109, 158, 172, 238
NlaIII CATG 6 cut(s) 479, 515, 656, 970, 980, 1006
NmuCI GTSAC 2 cut(s) 680, 907
NsbI TGCGCA 1 cut(s) 540
OliI CACNNNNGTG 1 cut(s) 302
PctI GAATGC 1 cut(s) 541
PfeI GAWTC 3 cut(s) 77, 137, 985
PinAI ACCGGT 1 cut(s) 674
PkrI GCNGC 9 cut(s) 100, 286, 443, 490, 538, 556, 608, 1030, 1033
PleI GAGTC 1 cut(s) 993
PluTI GGCGCC 1 cut(s) 510
PpsI GAGTC 1 cut(s) 993
Ppu21I YACGTR 1 cut(s) 595
PpuMI RGGWCCY 1 cut(s) 149
PshBI ATTAAT 1 cut(s) 870
PsiI TTATAA 1 cut(s) 758
Psp5II RGGWCCY 1 cut(s) 149
Psp6I CCWGG 2 cut(s) 198, 354
PspGI CCWGG 2 cut(s) 198, 354
PspOMI GGGCCC 1 cut(s) 345
PspPI GGNCC 8 cut(s) 4, 149, 345, 346, 659, 845, 889, 970
PspPPI RGGWCCY 1 cut(s) 149
PsuI RGATCY 2 cut(s) 158, 238
RsaI GTAC 2 cut(s) 437, 597
RsaNI GTAC 2 cut(s) 436, 596
RseI CAYNNNNRTG 3 cut(s) 302, 393, 516
SaqAI TTAA 1 cut(s) 870
SatI GCNGC 9 cut(s) 99, 285, 442, 489, 537, 555, 607, 1029, 1032
Sau3AI GATC 4 cut(s) 109, 158, 172, 238
Sau96I GGNCC 8 cut(s) 4, 149, 345, 346, 659, 845, 889, 970
SchI GAGTC 1 cut(s) 993
ScrFI CCNGG 4 cut(s) 142, 200, 349, 356
SduI GDGCHC 3 cut(s) 315, 349, 772
SetI ASST 9 cut(s) 34, 63, 109, 583, 588, 597, 764, 984, 1048
SfaNI GCATC 4 cut(s) 79, 541, 685, 1049
SfoI GGCGCC 1 cut(s) 508
SinI GGWCC 4 cut(s) 4, 149, 659, 889
SmiMI CAYNNNNRTG 3 cut(s) 302, 393, 516
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
Sse9I AATT 4 cut(s) 402, 524, 629, 871
SsiI CCGC 7 cut(s) 99, 236, 285, 377, 441, 607, 1031
SspDI GGCGCC 1 cut(s) 506
SspMI CTAG 3 cut(s) 447, 699, 849
StyD4I CCNGG 4 cut(s) 140, 198, 347, 354
StyI CCWWGG 4 cut(s) 114, 279, 511, 1035
TaaI ACNGT 3 cut(s) 301, 385, 686
TaiI ACGT 1 cut(s) 597
TaqI TCGA 5 cut(s) 63, 647, 669, 936, 988
TasI AATT 4 cut(s) 402, 524, 629, 871
TauI GCSGC 5 cut(s) 101, 287, 444, 609, 1034
TfiI GAWTC 3 cut(s) 77, 137, 985
Tru1I TTAA 1 cut(s) 870
Tru9I TTAA 1 cut(s) 870
TscAI CASTG 2 cut(s) 238, 306
TseFI GTSAC 2 cut(s) 680, 907
TseI GCWGC 4 cut(s) 488, 536, 554, 1028
Tsp45I GTSAC 2 cut(s) 680, 907
TspDTI ATGAA 4 cut(s) 42, 377, 464, 591
TspGWI ACGGA 3 cut(s) 70, 71, 219
TspRI CASTG 2 cut(s) 238, 306
VneI GTGCAC 1 cut(s) 311
VpaK11BI GGWCC 4 cut(s) 4, 149, 659, 889
VspI ATTAAT 1 cut(s) 870
XspI CTAG 3 cut(s) 447, 699, 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.