Rroxscaffold_1G00049420

poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0019421
Physical Location & Seq
Forward (+)
69597536 .. 69598123
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00049420.1

Sequence Viewer

Length: 588 bp
ATGCTAATGGTGGCGCCCATGGTTGTGTTCAATTGGTTCTGCCGCGCTTTCTTTGGATGCCGCATTGTTCTCGTGAAGTTGATGAAGCCGAGCTTAGGCAAGTACGGTGTGCCGGTCATACCGGGGAGGGTGCGGTTAGTCCCCGATGGCTCGAACCATGTTGGTCCCGATTTCGATACCGGTTATGACAGTTTTACCGATGCTAGGACACTTTATGTCCCTGCCGGCTCCGCCAACTCTCGCATTTGTGCTGATTTTGTTATAAGCTTTAGGGCTCCTTCTTGCTTATGGGATTATGCAACATATGCTACTCGGGCGAGTGGTGGTTTTGTTGGTGCTTCAAGGGGGCCTAGACCACAAGAACTACCCATTAATTTGTTTGCTGTGTTGGACCGGCTCGCAAGTTTTGTCACTCCTCTACAAATGACCATTGTCGTCAAGTTGTTTTGGAAGTTTAAGGGAAACAACATGAGCCGACACGAGCTGATTCGACAACCGAGACTCATGGTTGGTGAGGAGGCGTTGGTTGCAGCGGCCAAGGTGTCATGCCCTGAATTTTTTACACAATTTCAAAAATTCGAGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PARP RST

Protein Analysis

195

Amino Acids

21.74

Weight (kDa)

9.5

Isoelectric Point (pI)

32.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 119 - 180 3e-08 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019421)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0030821
rosa_laevigata RLG00000033257
rosa_multiflora Rmu_sc0001974.1_g000030
rosa_roxburghii Rroxscaffold_1G00049420
rosa_rugosa Rorug05G0121000
rosa_samantha Rh5BG212900 Rh5CG236200 Rh5DG217400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 263
AccB1I GGYRCC 1 cut(s) 13
AccII CGCG 1 cut(s) 45
AciI CCGC 5 cut(s) 43, 61, 133, 231, 533
AcoI YGGCCR 1 cut(s) 534
AcsI RAATTY 2 cut(s) 554, 575
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 1 cut(s) 104
AfiI CCNNNNNNNGG 2 cut(s) 95, 204
AgeI ACCGGT 1 cut(s) 179
AgsI TTSAA 3 cut(s) 31, 342, 572
AluBI AGCT 3 cut(s) 93, 267, 484
AluI AGCT 3 cut(s) 93, 267, 484
Alw26I GTCTC 2 cut(s) 493, 575
Ama87I CYCGRG 1 cut(s) 312
AoxI GGCC 2 cut(s) 347, 534
ApeKI GCWGC 1 cut(s) 530
ApoI RAATTY 2 cut(s) 554, 575
AseI ATTAAT 1 cut(s) 372
AsiGI ACCGGT 1 cut(s) 179
AspLEI GCGC 2 cut(s) 16, 47
AspS9I GGNCC 3 cut(s) 164, 347, 391
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 1 cut(s) 524
AvaI CYCGRG 1 cut(s) 312
AvaII GGWCC 2 cut(s) 164, 391
BanI GGYRCC 1 cut(s) 13
BanII GRGCYC 1 cut(s) 277
BauI CACGAG 2 cut(s) 71, 479
BbvI GCAGC 1 cut(s) 542
BccI CCATC 1 cut(s) 140
BcgI CGANNNNNNTGC 2 cut(s) 52, 86
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 2 cut(s) 493, 575
BfaI CTAG 2 cut(s) 204, 351
BfoI RGCGCY 1 cut(s) 17
BisI GCNGC 4 cut(s) 43, 61, 531, 534
BlsI GCNGC 4 cut(s) 44, 62, 532, 535
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 2 cut(s) 164, 391
BmeT110I CYCGRG 1 cut(s) 312
BmgT120I GGNCC 3 cut(s) 164, 347, 391
BmiI GGNNCC 5 cut(s) 15, 166, 229, 276, 348
BmrFI CCNGG 1 cut(s) 123
BmsI GCATC 2 cut(s) 47, 190
BoxI GACNNNNGTC 1 cut(s) 431
Bpu10I CCTNAGC 1 cut(s) 94
BpuMI CCSGG 1 cut(s) 123
BsaHI GRCGYC 1 cut(s) 14
BsaJI CCNNGG 3 cut(s) 18, 122, 537
BsaWI WCCGGW 1 cut(s) 179
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 204
Bse118I RCCGGY 4 cut(s) 112, 179, 224, 393
BseDI CCNNGG 3 cut(s) 18, 122, 537
BseGI GGATG 1 cut(s) 62
BseLI CCNNNNNNNGG 2 cut(s) 95, 204
BseRI GAGGAG 2 cut(s) 405, 530
BseXI GCAGC 1 cut(s) 542
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 2 cut(s) 349, 536
BshNI GGYRCC 1 cut(s) 13
BshTI ACCGGT 1 cut(s) 179
BsiHKCI CYCGRG 1 cut(s) 312
BsiSI CCGG 5 cut(s) 113, 122, 180, 225, 394
BslFI GGGAC 3 cut(s) 125, 150, 203
BslI CCNNNNNNNGG 2 cut(s) 95, 204
BsmAI GTCTC 2 cut(s) 493, 575
BsmFI GGGAC 3 cut(s) 125, 150, 203
BsnI GGCC 2 cut(s) 349, 536
BsoBI CYCGRG 1 cut(s) 312
Bsp1286I GDGCHC 1 cut(s) 277
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 5 cut(s) 43, 61, 133, 231, 533
BspANI GGCC 2 cut(s) 349, 536
BspFNI CGCG 1 cut(s) 45
BspLI GGNNCC 5 cut(s) 15, 166, 229, 276, 348
BspT107I GGYRCC 1 cut(s) 13
BsrFI RCCGGY 4 cut(s) 112, 179, 224, 393
BssAI RCCGGY 4 cut(s) 112, 179, 224, 393
BssECI CCNNGG 3 cut(s) 18, 122, 537
BssNI GRCGYC 1 cut(s) 14
BssSI CACGAG 2 cut(s) 71, 479
BssT1I CCWWGG 2 cut(s) 18, 537
Bst2BI CACGAG 2 cut(s) 71, 479
Bst4CI ACNGT 2 cut(s) 107, 191
BstACI GRCGYC 1 cut(s) 14
BstAPI GCANNNNNTGC 1 cut(s) 305
BstC8I GCNNGC 2 cut(s) 226, 399
BstDEI CTNAG 1 cut(s) 94
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 1 cut(s) 62
BstFNI CGCG 1 cut(s) 45
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 2 cut(s) 16, 47
BstMAI GTCTC 2 cut(s) 493, 575
BstMWI GCNNNNNNNGC 4 cut(s) 230, 305, 314, 527
BstPAI GACNNNNGTC 1 cut(s) 431
BstSCI CCNGG 1 cut(s) 121
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 1 cut(s) 542
BsuRI GGCC 2 cut(s) 349, 536
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 1 cut(s) 62
Cac8I GCNNGC 2 cut(s) 226, 399
CfoI GCGC 2 cut(s) 16, 47
Cfr10I RCCGGY 4 cut(s) 112, 179, 224, 393
Cfr13I GGNCC 3 cut(s) 164, 347, 391
Csp6I GTAC 1 cut(s) 103
CspAI ACCGGT 1 cut(s) 179
CviAII CATG 6 cut(s) 19, 158, 469, 505, 546, 585
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 1 cut(s) 94
DinI GGCGCC 1 cut(s) 15
EaeI YGGCCR 1 cut(s) 534
EciI GGCGGA 1 cut(s) 220
Eco130I CCWWGG 2 cut(s) 18, 537
Eco24I GRGCYC 1 cut(s) 277
Eco47I GGWCC 2 cut(s) 164, 391
Eco88I CYCGRG 1 cut(s) 312
EcoO109I RGGNCCY 1 cut(s) 347
EcoT14I CCWWGG 2 cut(s) 18, 537
EcoT38I GRGCYC 1 cut(s) 277
EgeI GGCGCC 1 cut(s) 15
EheI GGCGCC 1 cut(s) 15
ErhI CCWWGG 2 cut(s) 18, 537
FaeI CATG 6 cut(s) 22, 161, 472, 508, 549, 588
FalI AAGNNNNNCTT 2 cut(s) 77, 109
FaqI GGGAC 3 cut(s) 125, 150, 203
FatI CATG 6 cut(s) 18, 157, 468, 504, 545, 584
FauNDI CATATG 1 cut(s) 304
Fnu4HI GCNGC 4 cut(s) 43, 61, 531, 534
FokI GGATG 1 cut(s) 69
FriOI GRGCYC 1 cut(s) 277
Fsp4HI GCNGC 4 cut(s) 43, 61, 531, 534
FspBI CTAG 2 cut(s) 204, 351
GlaI GCGC 2 cut(s) 15, 46
GluI GCNGC 4 cut(s) 43, 61, 531, 534
HaeII RGCGCY 1 cut(s) 17
HaeIII GGCC 2 cut(s) 349, 536
HapII CCGG 5 cut(s) 113, 122, 180, 225, 394
HhaI GCGC 2 cut(s) 16, 47
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 6 cut(s) 22, 161, 472, 508, 549, 588
Hin6I GCGC 2 cut(s) 14, 45
HinP1I GCGC 2 cut(s) 14, 45
HindIII AAGCTT 1 cut(s) 265
HinfI GANTC 2 cut(s) 487, 501
HpaII CCGG 5 cut(s) 113, 122, 180, 225, 394
HphI GGTGA 1 cut(s) 524
Hpy188III TCNNGA 3 cut(s) 73, 167, 580
HpyAV CCTTC 1 cut(s) 288
HpyCH4III ACNGT 2 cut(s) 107, 191
HpyCH4V TGCA 2 cut(s) 299, 530
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 305, 314, 527
HpyF3I CTNAG 1 cut(s) 94
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 6 cut(s) 22, 161, 472, 508, 549, 588
HspAI GCGC 2 cut(s) 14, 45
KasI GGCGCC 1 cut(s) 13
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
LmnI GCTCC 2 cut(s) 233, 280
LpnPI CCDG 7 cut(s) 126, 135, 193, 234, 238, 407, 564
Lsp1109I GCAGC 1 cut(s) 542
LweI GCATC 2 cut(s) 47, 190
MaeI CTAG 2 cut(s) 204, 351
MaeIII GTNAC 1 cut(s) 409
MfeI CAATTG 1 cut(s) 31
MhlI GDGCHC 1 cut(s) 277
MluCI AATT 5 cut(s) 31, 373, 554, 566, 575
Mly113I GGCGCC 1 cut(s) 14
MlyI GAGTC 1 cut(s) 495
MmeI TCCRAC 1 cut(s) 369
MnlI CCTC 4 cut(s) 120, 426, 508, 511
MroNI GCCGGC 1 cut(s) 224
MseI TTAA 2 cut(s) 372, 456
MslI CAYNNNNRTG 1 cut(s) 23
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 5 cut(s) 113, 122, 180, 225, 394
MspR9I CCNGG 1 cut(s) 123
MunI CAATTG 1 cut(s) 31
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 4 cut(s) 230, 305, 314, 527
NaeI GCCGGC 1 cut(s) 226
NarI GGCGCC 1 cut(s) 14
NciI CCSGG 1 cut(s) 123
NcoI CCATGG 1 cut(s) 18
NdeI CATATG 1 cut(s) 304
NgoMIV GCCGGC 1 cut(s) 224
NlaIII CATG 6 cut(s) 22, 161, 472, 508, 549, 588
NlaIV GGNNCC 5 cut(s) 15, 166, 229, 276, 348
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 1 cut(s) 409
PdiI GCCGGC 1 cut(s) 226
PfeI GAWTC 1 cut(s) 487
PinAI ACCGGT 1 cut(s) 179
PkrI GCNGC 4 cut(s) 44, 62, 532, 535
PleI GAGTC 1 cut(s) 495
PluTI GGCGCC 1 cut(s) 17
PpsI GAGTC 1 cut(s) 495
PshAI GACNNNNGTC 1 cut(s) 431
PshBI ATTAAT 1 cut(s) 372
PsiI TTATAA 1 cut(s) 263
PspN4I GGNNCC 5 cut(s) 15, 166, 229, 276, 348
PspPI GGNCC 3 cut(s) 164, 347, 391
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
RseI CAYNNNNRTG 1 cut(s) 23
SaqAI TTAA 2 cut(s) 372, 456
SatI GCNGC 4 cut(s) 43, 61, 531, 534
Sau96I GGNCC 3 cut(s) 164, 347, 391
SchI GAGTC 1 cut(s) 495
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 1 cut(s) 277
SetI ASST 4 cut(s) 95, 269, 486, 543
SfaNI GCATC 2 cut(s) 47, 190
SfoI GGCGCC 1 cut(s) 15
SinI GGWCC 2 cut(s) 164, 391
SmiMI CAYNNNNRTG 1 cut(s) 23
Sse9I AATT 5 cut(s) 31, 373, 554, 566, 575
SsiI CCGC 5 cut(s) 43, 61, 133, 231, 533
SspDI GGCGCC 1 cut(s) 13
SspMI CTAG 2 cut(s) 204, 351
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 2 cut(s) 18, 537
TaaI ACNGT 2 cut(s) 107, 191
TaqI TCGA 4 cut(s) 152, 174, 490, 579
TasI AATT 5 cut(s) 31, 373, 554, 566, 575
TauI GCSGC 3 cut(s) 45, 63, 536
TfiI GAWTC 1 cut(s) 487
Tru1I TTAA 2 cut(s) 372, 456
Tru9I TTAA 2 cut(s) 372, 456
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 1 cut(s) 530
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 1 cut(s) 98
VpaK11BI GGWCC 2 cut(s) 164, 391
VspI ATTAAT 1 cut(s) 372
XapI RAATTY 2 cut(s) 554, 575
XspI CTAG 2 cut(s) 204, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.