Rh5BG212900

poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0019421
Physical Location & Seq
Reverse (-)
24651926 .. 24653238
1313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG212900.1

Sequence Viewer

Length: 1059 bp
ATGGAGCAGACCCAGATTGTTGAAAATGAAGCTTCTGGTTCCAATGTGGATATAACGGAGGTCGAGAAGCATCAAGATTCCGTTTCCGGTGCCGGTGTCGCCGAAGCTGATCCCAAGGCTGACAAGAACGATAAAGAATCCGGGTCGGGTCCTTCCGGATCCAGTTCCTTTGCTCCAGACCAGTTTAGGGTTCTCGCCAAGGACAGAACAATGTTGAGAGTAAGGGGAACAAGTGCGGATTTAATAATAGTCAAGAGGAACTTCAATTCCGGTATGGCCTTGGCCTCCGGAGAAACCACAGTGGTGGTTGTGCACAAGTATCCGGCTTCTAATCTGACCCATCGGGCCAGGTTCCAGGCTTTTCGGGCTTCCTTAGCGGAGACGGTTCATAAATATGGTGGAATTGTCCAAAATGCCCATCTCAAGACTGCGCTGTACGGCGGCTCTAAGGATGAGATTGCTCAGATTTTGGTTCATGGGTTTAGTGCTGCCAATGCTAATGGTGGCGCCCATGGTTGTGTTCAATTGGTTCCTGCTGCGCATTCTTTGGATGCTGCATTGTTCTGTGAAGTTGATGAAGTTGAGCTAAGGCACGTACTGGTGTGCTGCGTCATAATGGGGAGGGTGCAATTCGTCCCTGATGGCTCGAACCATGTTGGTCCCGATTTCGATACCGGTTGTGACAGTTTTACCGATGCTAGGACACTTTATGTGCCTGCCGGTTTAGCCGGCTCTCGCATTTGTGCTGATTTTGTTATAAGCTTCAGGGCTCCTTCTTGCTTATGGGATTATGCGACATATGCTACTCAGGCGAGTGGTGGTTTTGTTGGTGCTTCAAGGCGGCCTAGACCACAAGAACTACCCATTAATTTGTCTGCTGTGTTGGACCGGCTTGCAAGTTTTGTCACTCCTCTACAAATGACCATTGCGGTCGAGTTGTTTTGGAAGTATAAGGGAAACAACATGAGCCGACATGAGCTGATTCGACAACTGAGACTCATGGTTGGTGAGGAGGCATTGGTTGCAGCGGCCAAGGCATCAGCTTCGGTGAACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PARP RST

Protein Analysis

352

Amino Acids

37.68

Weight (kDa)

6.26

Isoelectric Point (pI)

29.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 284 - 345 2.7e-08 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019421)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0030821
rosa_laevigata RLG00000033257
rosa_multiflora Rmu_sc0001974.1_g000030
rosa_roxburghii Rroxscaffold_1G00049420
rosa_rugosa Rorug05G0121000
rosa_samantha Rh5BG212900 Rh5CG236200 Rh5DG217400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 758
Acc16I TGCGCA 1 cut(s) 540
AccB1I GGYRCC 2 cut(s) 89, 506
AccIII TCCGGA 2 cut(s) 155, 287
AciI CCGC 6 cut(s) 236, 377, 441, 841, 929, 1028
AclWI GGATC 3 cut(s) 104, 153, 166
AcoI YGGCCR 1 cut(s) 1029
AcuI CTGAAG 1 cut(s) 748
AcyI GRCGYC 1 cut(s) 507
AfaI GTAC 2 cut(s) 437, 597
AfiI CCNNNNNNNGG 2 cut(s) 187, 699
AgeI ACCGGT 1 cut(s) 674
AgsI TTSAA 4 cut(s) 23, 265, 524, 837
AjnI CCWGG 2 cut(s) 347, 354
AleI CACNNNNGTG 1 cut(s) 302
AloI GAACNNNNNNTCC 2 cut(s) 251, 283
AluBI AGCT 6 cut(s) 32, 107, 586, 762, 979, 1043
AluI AGCT 6 cut(s) 32, 107, 586, 762, 979, 1043
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 374, 988
Alw44I GTGCAC 1 cut(s) 311
AlwI GGATC 3 cut(s) 104, 153, 166
Aor13HI TCCGGA 2 cut(s) 155, 287
AoxI GGCC 5 cut(s) 276, 282, 345, 842, 1029
ApaLI GTGCAC 1 cut(s) 311
ApeKI GCWGC 5 cut(s) 488, 536, 554, 606, 1025
AseI ATTAAT 1 cut(s) 867
AsiGI ACCGGT 1 cut(s) 674
AspLEI GCGC 3 cut(s) 433, 509, 541
AspS9I GGNCC 4 cut(s) 149, 345, 659, 886
AsuC2I CCSGG 1 cut(s) 142
AsuHPI GGTGA 1 cut(s) 1019
AvaII GGWCC 3 cut(s) 149, 659, 886
BaeGI GKGCMC 1 cut(s) 315
BamHI GGATCC 1 cut(s) 158
BanI GGYRCC 2 cut(s) 89, 506
BanII GRGCYC 1 cut(s) 772
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 5 cut(s) 475, 523, 541, 593, 1037
BccI CCATC 3 cut(s) 348, 426, 635
BceAI ACGGC 1 cut(s) 454
BcgI CGANNNNNNTGC 1 cut(s) 1026
BciT130I CCWGG 2 cut(s) 349, 356
BciVI GTATCC 1 cut(s) 330
BcnI CCSGG 1 cut(s) 142
BcoDI GTCTC 2 cut(s) 374, 988
BfaI CTAG 2 cut(s) 699, 846
BfoI RGCGCY 1 cut(s) 510
BfuI GTATCC 1 cut(s) 330
BisI GCNGC 8 cut(s) 442, 489, 537, 555, 607, 842, 1026, 1029
BlsI GCNGC 8 cut(s) 443, 490, 538, 556, 608, 843, 1027, 1030
Bme1390I CCNGG 3 cut(s) 142, 349, 356
Bme18I GGWCC 3 cut(s) 149, 659, 886
BmgT120I GGNCC 4 cut(s) 149, 345, 659, 886
BmiI GGNNCC 9 cut(s) 40, 91, 150, 160, 353, 508, 531, 661, 771
BmrFI CCNGG 3 cut(s) 142, 349, 356
BmsI GCATC 4 cut(s) 79, 541, 685, 1046
BpmI CTGGAG 1 cut(s) 159
Bpu10I CCTNAGC 2 cut(s) 373, 587
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 1 cut(s) 142
BsaAI YACGTR 1 cut(s) 595
BsaHI GRCGYC 1 cut(s) 507
BsaJI CCNNGG 5 cut(s) 114, 198, 279, 511, 1032
BsaWI WCCGGW 5 cut(s) 86, 155, 269, 287, 674
Bsc4I CCNNNNNNNGG 2 cut(s) 187, 699
Bse118I RCCGGY 5 cut(s) 92, 674, 719, 728, 888
Bse1I ACTGG 3 cut(s) 162, 181, 603
Bse3DI GCAATG 1 cut(s) 924
BseAI TCCGGA 2 cut(s) 155, 287
BseBI CCWGG 2 cut(s) 349, 356
BseDI CCNNGG 5 cut(s) 114, 198, 279, 511, 1032
BseGI GGATG 2 cut(s) 457, 556
BseLI CCNNNNNNNGG 2 cut(s) 187, 699
BseMI GCAATG 1 cut(s) 924
BseMII CTCAG 3 cut(s) 476, 821, 983
BseNI ACTGG 3 cut(s) 162, 181, 603
BseRI GAGGAG 2 cut(s) 900, 1025
BseSI GKGCMC 1 cut(s) 315
BseXI GCAGC 5 cut(s) 475, 523, 541, 593, 1037
Bsh1285I CGRYCG 1 cut(s) 933
BshFI GGCC 5 cut(s) 278, 284, 347, 844, 1031
BshNI GGYRCC 2 cut(s) 89, 506
BshTI ACCGGT 1 cut(s) 674
BsiEI CGRYCG 1 cut(s) 933
BsiHKAI GWGCWC 1 cut(s) 315
BslFI GGGAC 2 cut(s) 620, 645
BslI CCNNNNNNNGG 2 cut(s) 187, 699
BsmAI GTCTC 2 cut(s) 374, 988
BsmBI CGTCTC 1 cut(s) 374
BsmFI GGGAC 2 cut(s) 620, 645
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 5 cut(s) 278, 284, 347, 844, 1031
Bsp1286I GDGCHC 2 cut(s) 315, 772
Bsp13I TCCGGA 2 cut(s) 155, 287
Bsp143I GATC 2 cut(s) 109, 158
Bsp19I CCATGG 1 cut(s) 511
BspACI CCGC 6 cut(s) 236, 377, 441, 841, 929, 1028
BspANI GGCC 5 cut(s) 278, 284, 347, 844, 1031
BspCNI CTCAG 3 cut(s) 475, 820, 984
BspEI TCCGGA 2 cut(s) 155, 287
BspLI GGNNCC 9 cut(s) 40, 91, 150, 160, 353, 508, 531, 661, 771
BspPI GGATC 3 cut(s) 104, 153, 166
BspT107I GGYRCC 2 cut(s) 89, 506
BsrDI GCAATG 1 cut(s) 924
BsrFI RCCGGY 5 cut(s) 92, 674, 719, 728, 888
BsrI ACTGG 3 cut(s) 162, 181, 603
BssAI RCCGGY 5 cut(s) 92, 674, 719, 728, 888
BssECI CCNNGG 5 cut(s) 114, 198, 279, 511, 1032
BssMI GATC 2 cut(s) 109, 158
BssNI GRCGYC 1 cut(s) 507
BssT1I CCWWGG 5 cut(s) 114, 198, 279, 511, 1032
Bst2UI CCWGG 2 cut(s) 349, 356
Bst4CI ACNGT 3 cut(s) 301, 385, 686
BstACI GRCGYC 1 cut(s) 507
BstAPI GCANNNNNTGC 1 cut(s) 1022
BstBAI YACGTR 1 cut(s) 595
BstC8I GCNNGC 3 cut(s) 717, 730, 894
BstDEI CTNAG 6 cut(s) 373, 447, 462, 587, 807, 992
BstDSI CCRYGG 1 cut(s) 511
BstF5I GGATG 2 cut(s) 457, 556
BstH2I RGCGCY 1 cut(s) 510
BstHHI GCGC 3 cut(s) 433, 509, 541
BstKTI GATC 2 cut(s) 112, 161
BstMAI GTCTC 2 cut(s) 374, 988
BstMBI GATC 2 cut(s) 109, 158
BstMCI CGRYCG 1 cut(s) 933
BstMWI GCNNNNNNNGC 9 cut(s) 98, 365, 374, 494, 725, 800, 809, 1022, 1034
BstNI CCWGG 2 cut(s) 349, 356
BstSCI CCNGG 3 cut(s) 140, 347, 354
BstSLI GKGCMC 1 cut(s) 315
BstV1I GCAGC 5 cut(s) 475, 523, 541, 593, 1037
BstX2I RGATCY 1 cut(s) 158
BstXI CCANNNNNNTGG 1 cut(s) 304
BstYI RGATCY 1 cut(s) 158
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 5 cut(s) 278, 284, 347, 844, 1031
BtgI CCRYGG 1 cut(s) 511
BtsCI GGATG 2 cut(s) 457, 556
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 3 cut(s) 717, 730, 894
CfoI GCGC 3 cut(s) 433, 509, 541
Cfr10I RCCGGY 5 cut(s) 92, 674, 719, 728, 888
Cfr13I GGNCC 4 cut(s) 149, 345, 659, 886
CseI GACGC 1 cut(s) 598
Csp6I GTAC 2 cut(s) 436, 596
CspAI ACCGGT 1 cut(s) 674
CviAII CATG 6 cut(s) 476, 512, 653, 964, 974, 1000
CviQI GTAC 2 cut(s) 436, 596
DdeI CTNAG 6 cut(s) 373, 447, 462, 587, 807, 992
DinI GGCGCC 1 cut(s) 508
DpnI GATC 2 cut(s) 111, 160
DpnII GATC 2 cut(s) 109, 158
EaeI YGGCCR 1 cut(s) 1029
Eco130I CCWWGG 5 cut(s) 114, 198, 279, 511, 1032
Eco24I GRGCYC 1 cut(s) 772
Eco47I GGWCC 3 cut(s) 149, 659, 886
Eco57I CTGAAG 1 cut(s) 748
EcoO109I RGGNCCY 1 cut(s) 149
EcoRII CCWGG 2 cut(s) 347, 354
EcoT14I CCWWGG 5 cut(s) 114, 198, 279, 511, 1032
EcoT38I GRGCYC 1 cut(s) 772
EgeI GGCGCC 1 cut(s) 508
EheI GGCGCC 1 cut(s) 508
ErhI CCWWGG 5 cut(s) 114, 198, 279, 511, 1032
Esp3I CGTCTC 1 cut(s) 374
FaeI CATG 6 cut(s) 479, 515, 656, 967, 977, 1003
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 2 cut(s) 620, 645
FatI CATG 6 cut(s) 475, 511, 652, 963, 973, 999
FauNDI CATATG 1 cut(s) 799
Fnu4HI GCNGC 8 cut(s) 442, 489, 537, 555, 607, 842, 1026, 1029
FokI GGATG 2 cut(s) 464, 563
FriOI GRGCYC 1 cut(s) 772
Fsp4HI GCNGC 8 cut(s) 442, 489, 537, 555, 607, 842, 1026, 1029
FspBI CTAG 2 cut(s) 699, 846
FspI TGCGCA 1 cut(s) 540
GlaI GCGC 3 cut(s) 432, 508, 540
GluI GCNGC 8 cut(s) 442, 489, 537, 555, 607, 842, 1026, 1029
GsuI CTGGAG 1 cut(s) 159
HaeII RGCGCY 1 cut(s) 510
HaeIII GGCC 5 cut(s) 278, 284, 347, 844, 1031
HgaI GACGC 1 cut(s) 598
HhaI GCGC 3 cut(s) 433, 509, 541
Hin1I GRCGYC 1 cut(s) 507
Hin1II CATG 6 cut(s) 479, 515, 656, 967, 977, 1003
Hin6I GCGC 3 cut(s) 431, 507, 539
HinP1I GCGC 3 cut(s) 431, 507, 539
HindIII AAGCTT 2 cut(s) 30, 760
HinfI GANTC 4 cut(s) 77, 137, 982, 996
HphI GGTGA 1 cut(s) 1019
Hpy166II GTNNAC 2 cut(s) 313, 1051
Hpy188I TCNGA 2 cut(s) 336, 465
Hpy188III TCNNGA 8 cut(s) 64, 74, 156, 176, 253, 288, 424, 662
Hpy8I GTNNAC 2 cut(s) 313, 1051
HpyAV CCTTC 2 cut(s) 162, 783
HpyCH4III ACNGT 3 cut(s) 301, 385, 686
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 5 cut(s) 313, 557, 628, 896, 1025
HpyF10VI GCNNNNNNNGC 9 cut(s) 98, 365, 374, 494, 725, 800, 809, 1022, 1034
HpyF3I CTNAG 6 cut(s) 373, 447, 462, 587, 807, 992
HpySE526I ACGT 1 cut(s) 594
Hsp92I GRCGYC 1 cut(s) 507
Hsp92II CATG 6 cut(s) 479, 515, 656, 967, 977, 1003
HspAI GCGC 3 cut(s) 431, 507, 539
KasI GGCGCC 1 cut(s) 506
Kpn2I TCCGGA 2 cut(s) 155, 287
KroI GCCGGC 1 cut(s) 728
KroNI GCCGGC 1 cut(s) 730
Kzo9I GATC 2 cut(s) 109, 158
LmnI GCTCC 3 cut(s) 4, 178, 775
Lsp1109I GCAGC 5 cut(s) 475, 523, 541, 593, 1037
LweI GCATC 4 cut(s) 79, 541, 685, 1046
MaeI CTAG 2 cut(s) 699, 846
MaeII ACGT 1 cut(s) 594
MaeIII GTNAC 2 cut(s) 680, 904
MalI GATC 2 cut(s) 111, 160
MboI GATC 2 cut(s) 109, 158
MfeI CAATTG 1 cut(s) 524
MflI RGATCY 1 cut(s) 158
MhlI GDGCHC 2 cut(s) 315, 772
MluCI AATT 5 cut(s) 265, 402, 524, 629, 868
Mly113I GGCGCC 1 cut(s) 507
MlyI GAGTC 1 cut(s) 990
MmeI TCCRAC 1 cut(s) 864
MnlI CCTC 7 cut(s) 52, 249, 295, 615, 921, 1003, 1006
MroI TCCGGA 2 cut(s) 155, 287
MroNI GCCGGC 1 cut(s) 728
MseI TTAA 2 cut(s) 242, 867
MslI CAYNNNNRTG 3 cut(s) 302, 393, 516
MspA1I CMGCKG 1 cut(s) 1028
MspR9I CCNGG 3 cut(s) 142, 349, 356
MunI CAATTG 1 cut(s) 524
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 2 cut(s) 349, 356
MwoI GCNNNNNNNGC 9 cut(s) 98, 365, 374, 494, 725, 800, 809, 1022, 1034
NaeI GCCGGC 1 cut(s) 730
NarI GGCGCC 1 cut(s) 507
NciI CCSGG 1 cut(s) 142
NcoI CCATGG 1 cut(s) 511
NdeI CATATG 1 cut(s) 799
NdeII GATC 2 cut(s) 109, 158
NgoMIV GCCGGC 1 cut(s) 728
NlaIII CATG 6 cut(s) 479, 515, 656, 967, 977, 1003
NlaIV GGNNCC 9 cut(s) 40, 91, 150, 160, 353, 508, 531, 661, 771
NmuCI GTSAC 2 cut(s) 680, 904
NsbI TGCGCA 1 cut(s) 540
OliI CACNNNNGTG 1 cut(s) 302
PctI GAATGC 1 cut(s) 541
PdiI GCCGGC 1 cut(s) 730
PfeI GAWTC 3 cut(s) 77, 137, 982
PinAI ACCGGT 1 cut(s) 674
PkrI GCNGC 8 cut(s) 443, 490, 538, 556, 608, 843, 1027, 1030
PleI GAGTC 1 cut(s) 990
PluTI GGCGCC 1 cut(s) 510
PpsI GAGTC 1 cut(s) 990
Ppu21I YACGTR 1 cut(s) 595
PpuMI RGGWCCY 1 cut(s) 149
PshBI ATTAAT 1 cut(s) 867
PsiI TTATAA 1 cut(s) 758
Psp5II RGGWCCY 1 cut(s) 149
Psp6I CCWGG 2 cut(s) 347, 354
PspGI CCWGG 2 cut(s) 347, 354
PspN4I GGNNCC 9 cut(s) 40, 91, 150, 160, 353, 508, 531, 661, 771
PspPI GGNCC 4 cut(s) 149, 345, 659, 886
PspPPI RGGWCCY 1 cut(s) 149
PsuI RGATCY 1 cut(s) 158
RsaI GTAC 2 cut(s) 437, 597
RsaNI GTAC 2 cut(s) 436, 596
RseI CAYNNNNRTG 3 cut(s) 302, 393, 516
SaqAI TTAA 2 cut(s) 242, 867
SatI GCNGC 8 cut(s) 442, 489, 537, 555, 607, 842, 1026, 1029
Sau3AI GATC 2 cut(s) 109, 158
Sau96I GGNCC 4 cut(s) 149, 345, 659, 886
SchI GAGTC 1 cut(s) 990
ScrFI CCNGG 3 cut(s) 142, 349, 356
SduI GDGCHC 2 cut(s) 315, 772
SetI ASST 9 cut(s) 34, 63, 109, 353, 588, 597, 764, 981, 1045
SfaNI GCATC 4 cut(s) 79, 541, 685, 1046
SfoI GGCGCC 1 cut(s) 508
SinI GGWCC 3 cut(s) 149, 659, 886
SmiMI CAYNNNNRTG 3 cut(s) 302, 393, 516
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
Sse9I AATT 5 cut(s) 265, 402, 524, 629, 868
SsiI CCGC 6 cut(s) 236, 377, 441, 841, 929, 1028
SspDI GGCGCC 1 cut(s) 506
SspMI CTAG 2 cut(s) 699, 846
StyD4I CCNGG 3 cut(s) 140, 347, 354
StyI CCWWGG 5 cut(s) 114, 198, 279, 511, 1032
TaaI ACNGT 3 cut(s) 301, 385, 686
TaiI ACGT 1 cut(s) 597
TaqI TCGA 5 cut(s) 63, 647, 669, 933, 985
TasI AATT 5 cut(s) 265, 402, 524, 629, 868
TauI GCSGC 3 cut(s) 444, 844, 1031
TfiI GAWTC 3 cut(s) 77, 137, 982
Tru1I TTAA 2 cut(s) 242, 867
Tru9I TTAA 2 cut(s) 242, 867
TscAI CASTG 1 cut(s) 306
TseFI GTSAC 2 cut(s) 680, 904
TseI GCWGC 5 cut(s) 488, 536, 554, 606, 1025
Tsp45I GTSAC 2 cut(s) 680, 904
TspDTI ATGAA 4 cut(s) 42, 377, 464, 591
TspGWI ACGGA 2 cut(s) 70, 71
TspRI CASTG 1 cut(s) 306
VneI GTGCAC 1 cut(s) 311
VpaK11BI GGWCC 3 cut(s) 149, 659, 886
VspI ATTAAT 1 cut(s) 867
XspI CTAG 2 cut(s) 699, 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.