RchiOBHm_Chr5g0052821

A G-specific adenine DNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011052
Physical Location & Seq
Forward (+)
54962279 .. 54969097
6819 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33019

Sequence Viewer

Length: 1368 bp
ATGGTAGTCAAGAAGAAGAAGCAGGCCGCCAATGCAGCCGTTGCCAACAGAAGGCAAACCCTAAGGCGGTGTCGATCGGCCAAGCAACCAGAATTACAAGACATAGAAGACTTGTTCAGCGAAGACGAGACCGAGAAAATCAGGGAGGCTCTGCTGAAATGGTACGGCCTCAACGGCAGAGACCTGCCTTGGAGAGAAGAAGAGGAAGACGTGGAGGTCAGAGTGTACAGGGTGTGGGTCTCGGAAGTCATGCTTCAGCAGACTAGGGTTCAGACTGTGATTCAGTACTTCAACCGTTGGATGAGCAAATGGCCCACCATTCACAGCCTGGCCCAGGCTTCTCTTGAGGAGGTGAATGAAATGTGGGCTGGTTTGGGATACTATCGAAGAGCACGATTTCTTTTAGAGGGAGCTAGGAAGATTGTTGCAGAAGGAGATCAATTTCCTAAAACAGTTTCTCAGCTACGGAAAATACCAGGAATCGGAGACTATACGGCTGGAGCAATTGCCTCAATTGCATTAAAAGAGGCAGTTCCAGTGGTGGATGGAAATGTAATAAGGGTGATTGCTAGACTAAAGGCTATTTCTGCCAATCCAAAAGACTCTTTGACTGTCAAGAAATTTTGGAAACTAGCAGCCCAATTAGTTGACCCTTTCCAGCCTGGGGACTTCAATCAGGCTCTAATGGAGCTTGGTGCAACTTTATGTACGCCAACAAGTCCAAGCTGCGGCACATGTCCTGTATCTGACCAATGTTGTGCATTGTCAATATCTATGCATGATAGTTCAGTGGTGGTTACAGATTATCCGGTTAAGGTGGTAAAGACCAAACAAAGACATGATTTTTCTGCTGTGTGTGTTGTGGAAATATTGGGAGATGAGGAATCATCAAACGGACACCAAATCAACAATAAGTTTCTTCTGGTAAAAAGGCCAGATGAAGGGTTGCTTGCTGGCCTCTGGGAGTTCCCATCTGTCTCATTGGCTGGAGAAGTTGGCTTGGTTCCTAGGAGAAAGGCAATTGATCAATATTTGAATAAGTATTTCACACTCAACCCCAGAAAGACGTGTGACATAGTTTCCAGGGAACATGTGGGAGAATATGTCCATGTTTTCTCTCATATTCGACTCAAGATGTATGTGGAATCGTTGATTCTATGTGTTGAAGGTGGGATGAATGACCTGGTTAGCATGCGGGACAAAGAAATCGTGCCTTGGAAATGTGTGGATGCTGAGGTTCTTTCAAACATGGGATTGACATCTGGTGTAAGAAAGGTATACACTATGGTTCAAAAGTTAAAGCAAGAAAAGTCGTCTTCTGATCCTGATTTGGACATCAAGAAGAGAAAAAGGCCTTCAATAAAGTAG

Protein Analysis

455

Amino Acids

51.44

Weight (kDa)

8.9

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HhH-GPD PF00730 80 - 212 3.9e-25 HhH-GPD superfamily base excision DNA repair protein
HHH PF00633 146 - 172 2.9e-06 Helix-hairpin-helix motif
NUDIX_4 PF14815 303 - 427 5.2e-12 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12740 AT4G12740 AT4G12740
fragaria_vesca FvH4_3g28500 FvH4_3g28500 FvH4_3g28500 FvH4_3g28500
malus_domestica MD15G1141300.v1.1
prunus_persica Prupe.6G214800_v2.0.a1
pyrus_communis pycom15g12760
rosa_chinensis RchiOBHm_Chr5g0052821
rosa_laevigata RLG00000034790
rosa_multiflora Rmu_ssc0000311.1_g000017
rosa_roxburghii Rroxscaffold_1G00028640
rosa_rugosa Rorug05G0269500
rosa_samantha Rh5AG341500 Rh5BG352200 Rh5CG378900 Rh5DG368500
rosa_wichuraiana Rw5G032200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
Acc36I ACCTGC 1 cut(s) 192
AccI GTMKAC 1 cut(s) 1278
AciI CCGC 4 cut(s) 27, 67, 729, 1195
AclWI GGATC 1 cut(s) 1316
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 1 cut(s) 620
AcuI CTGAAG 1 cut(s) 239
AfaI GTAC 4 cut(s) 164, 227, 287, 709
AfiI CCNNNNNNNGG 7 cut(s) 51, 66, 334, 482, 664, 728, 941
AflIII ACRYGT 3 cut(s) 734, 1067, 1090
AgsI TTSAA 7 cut(s) 292, 673, 1036, 1166, 1245, 1292, 1359
AjiI CACGTC 2 cut(s) 211, 1068
AjnI CCWGG 6 cut(s) 327, 333, 475, 661, 1082, 1182
AluBI AGCT 4 cut(s) 413, 463, 691, 726
AluI AGCT 4 cut(s) 413, 463, 691, 726
Alw21I GWGCWC 1 cut(s) 394
Alw26I GTCTC 5 cut(s) 122, 174, 244, 480, 982
AlwI GGATC 1 cut(s) 1316
AoxI GGCC 8 cut(s) 24, 78, 166, 311, 330, 932, 955, 1352
ApeKI GCWGC 3 cut(s) 35, 635, 726
ApoI RAATTY 1 cut(s) 620
AspA2I CCTAGG 1 cut(s) 1007
AspS9I GGNCC 2 cut(s) 312, 331
AsuHPI GGTGA 2 cut(s) 364, 574
AvrII CCTAGG 1 cut(s) 1007
AxyI CCTNAGG 1 cut(s) 62
BbsI GAAGAC 4 cut(s) 114, 129, 213, 1308
Bbv12I GWGCWC 1 cut(s) 394
BbvCI CCTCAGC 1 cut(s) 1233
BbvI GCAGC 3 cut(s) 47, 647, 713
BccI CCATC 2 cut(s) 539, 979
BceAI ACGGC 4 cut(s) 23, 181, 190, 510
BciT130I CCWGG 6 cut(s) 329, 335, 477, 663, 1084, 1184
BciVI GTATCC 1 cut(s) 371
BclI TGATCA 1 cut(s) 1024
BcoDI GTCTC 5 cut(s) 122, 174, 244, 480, 982
BfaI CTAG 5 cut(s) 264, 414, 570, 632, 1008
BfuAI ACCTGC 1 cut(s) 192
BfuI GTATCC 1 cut(s) 371
BglI GCCNNNNNGGC 1 cut(s) 174
BisI GCNGC 5 cut(s) 27, 36, 636, 727, 730
BlnI CCTAGG 1 cut(s) 1007
BlsI GCNGC 5 cut(s) 28, 37, 637, 728, 731
BmcAI AGTACT 1 cut(s) 287
Bme1390I CCNGG 6 cut(s) 329, 335, 477, 663, 1084, 1184
BmgBI CACGTC 2 cut(s) 211, 1068
BmgT120I GGNCC 2 cut(s) 312, 331
BmiI GGNNCC 1 cut(s) 1005
BmrFI CCNGG 6 cut(s) 329, 335, 477, 663, 1084, 1184
BmsI GCATC 1 cut(s) 1219
BpiI GAAGAC 4 cut(s) 114, 129, 213, 1308
BpmI CTGGAG 2 cut(s) 519, 1008
Bpu10I CCTNAGC 1 cut(s) 1233
BpuEI CTTGAG 2 cut(s) 365, 1115
BsaBI GATNNNNATC 1 cut(s) 1258
BsaI GGTCTC 3 cut(s) 122, 174, 244
BsaJI CCNNGG 6 cut(s) 188, 333, 662, 1007, 1083, 1214
BsaWI WCCGGW 1 cut(s) 808
BsaXI ACNNNNNCTCC 2 cut(s) 1089, 1119
Bsc4I CCNNNNNNNGG 7 cut(s) 51, 66, 334, 482, 664, 728, 941
Bse1I ACTGG 1 cut(s) 536
Bse21I CCTNAGG 1 cut(s) 62
Bse8I GATNNNNATC 1 cut(s) 1258
BseBI CCWGG 6 cut(s) 329, 335, 477, 663, 1084, 1184
BseDI CCNNGG 6 cut(s) 188, 333, 662, 1007, 1083, 1214
BseGI GGATG 4 cut(s) 306, 550, 1179, 1234
BseJI GATNNNNATC 1 cut(s) 1258
BseLI CCNNNNNNNGG 7 cut(s) 51, 66, 334, 482, 664, 728, 941
BseMII CTCAG 2 cut(s) 473, 1224
BseNI ACTGG 1 cut(s) 536
BseRI GAGGAG 1 cut(s) 362
BseXI GCAGC 3 cut(s) 47, 647, 713
Bsh1285I CGRYCG 1 cut(s) 77
BshFI GGCC 8 cut(s) 26, 80, 168, 313, 332, 934, 957, 1354
BsiEI CGRYCG 1 cut(s) 77
BsiHKAI GWGCWC 1 cut(s) 394
BsiSI CCGG 1 cut(s) 809
BslFI GGGAC 2 cut(s) 680, 1211
BslI CCNNNNNNNGG 7 cut(s) 51, 66, 334, 482, 664, 728, 941
BsmAI GTCTC 5 cut(s) 122, 174, 244, 480, 982
BsmFI GGGAC 2 cut(s) 680, 1211
BsnI GGCC 8 cut(s) 26, 80, 168, 313, 332, 934, 957, 1354
Bso31I GGTCTC 3 cut(s) 122, 174, 244
Bsp1286I GDGCHC 1 cut(s) 394
Bsp1407I TGTACA 1 cut(s) 225
Bsp143I GATC 4 cut(s) 74, 436, 1024, 1321
BspACI CCGC 4 cut(s) 27, 67, 729, 1195
BspANI GGCC 8 cut(s) 26, 80, 168, 313, 332, 934, 957, 1354
BspCNI CTCAG 2 cut(s) 472, 1225
BspLI GGNNCC 1 cut(s) 1005
BspMI ACCTGC 1 cut(s) 192
BspPI GGATC 1 cut(s) 1316
BspQI GCTCTTC 1 cut(s) 382
BspTNI GGTCTC 3 cut(s) 122, 174, 244
BsrGI TGTACA 1 cut(s) 225
BsrI ACTGG 1 cut(s) 536
BssECI CCNNGG 6 cut(s) 188, 333, 662, 1007, 1083, 1214
BssMI GATC 4 cut(s) 74, 436, 1024, 1321
BssNAI GTATAC 1 cut(s) 1279
BssT1I CCWWGG 3 cut(s) 188, 1007, 1214
Bst1107I GTATAC 1 cut(s) 1279
Bst2UI CCWGG 6 cut(s) 329, 335, 477, 663, 1084, 1184
Bst4CI ACNGT 4 cut(s) 277, 296, 454, 613
Bst6I CTCTTC 3 cut(s) 195, 382, 1337
BstAPI GCANNNNNTGC 1 cut(s) 41
BstAUI TGTACA 1 cut(s) 225
BstC8I GCNNGC 4 cut(s) 24, 951, 955, 1193
BstDEI CTNAG 3 cut(s) 62, 459, 1233
BstENI CCTNNNNNAGG 1 cut(s) 332
BstF5I GGATG 4 cut(s) 306, 550, 1179, 1234
BstKTI GATC 4 cut(s) 77, 439, 1027, 1324
BstMAI GTCTC 5 cut(s) 122, 174, 244, 480, 982
BstMBI GATC 4 cut(s) 74, 436, 1024, 1321
BstMCI CGRYCG 1 cut(s) 77
BstMWI GCNNNNNNNGC 6 cut(s) 32, 35, 41, 174, 515, 587
BstNI CCWGG 6 cut(s) 329, 335, 477, 663, 1084, 1184
BstNSI RCATGY 3 cut(s) 738, 1094, 1195
BstSCI CCNGG 6 cut(s) 327, 333, 475, 661, 1082, 1182
BstV1I GCAGC 3 cut(s) 47, 647, 713
BstV2I GAAGAC 4 cut(s) 114, 129, 213, 1308
BstZ17I GTATAC 1 cut(s) 1279
Bsu36I CCTNAGG 1 cut(s) 62
BsuI GTATCC 1 cut(s) 371
BsuRI GGCC 8 cut(s) 26, 80, 168, 313, 332, 934, 957, 1354
BtrI CACGTC 2 cut(s) 211, 1068
BtsCI GGATG 4 cut(s) 306, 550, 1179, 1234
BtsIMutI CAGTG 2 cut(s) 543, 795
BveI ACCTGC 1 cut(s) 192
Cac8I GCNNGC 4 cut(s) 24, 951, 955, 1193
Cfr13I GGNCC 2 cut(s) 312, 331
CsiI ACCWGGT 1 cut(s) 1182
Csp6I GTAC 4 cut(s) 163, 226, 286, 708
CviAII CATG 8 cut(s) 250, 735, 779, 839, 1091, 1109, 1192, 1249
CviQI GTAC 4 cut(s) 163, 226, 286, 708
DdeI CTNAG 3 cut(s) 62, 459, 1233
DpnI GATC 4 cut(s) 76, 438, 1026, 1323
DpnII GATC 4 cut(s) 74, 436, 1024, 1321
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 3 cut(s) 195, 382, 1337
EarI CTCTTC 3 cut(s) 195, 382, 1337
Eco130I CCWWGG 3 cut(s) 188, 1007, 1214
Eco147I AGGCCT 1 cut(s) 1354
Eco31I GGTCTC 3 cut(s) 122, 174, 244
Eco57I CTGAAG 1 cut(s) 239
Eco81I CCTNAGG 1 cut(s) 62
EcoNI CCTNNNNNAGG 1 cut(s) 332
EcoRII CCWGG 6 cut(s) 327, 333, 475, 661, 1082, 1182
EcoT14I CCWWGG 3 cut(s) 188, 1007, 1214
EcoT22I ATGCAT 1 cut(s) 780
ErhI CCWWGG 3 cut(s) 188, 1007, 1214
FaeI CATG 8 cut(s) 253, 738, 782, 842, 1094, 1112, 1195, 1252
FalI AAGNNNNNCTT 4 cut(s) 237, 269, 933, 965
FaqI GGGAC 2 cut(s) 680, 1211
FatI CATG 8 cut(s) 249, 734, 778, 838, 1090, 1108, 1191, 1248
FauI CCCGC 1 cut(s) 1188
FbaI TGATCA 1 cut(s) 1024
FblI GTMKAC 1 cut(s) 1278
Fnu4HI GCNGC 5 cut(s) 27, 36, 636, 727, 730
FokI GGATG 4 cut(s) 313, 557, 1186, 1241
Fsp4HI GCNGC 5 cut(s) 27, 36, 636, 727, 730
FspBI CTAG 5 cut(s) 264, 414, 570, 632, 1008
GluI GCNGC 5 cut(s) 27, 36, 636, 727, 730
GsuI CTGGAG 2 cut(s) 519, 1008
HaeIII GGCC 8 cut(s) 26, 80, 168, 313, 332, 934, 957, 1354
HapII CCGG 1 cut(s) 809
Hin1II CATG 8 cut(s) 253, 738, 782, 842, 1094, 1112, 1195, 1252
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HinfI GANTC 7 cut(s) 280, 480, 602, 884, 1128, 1145, 1153
HpaII CCGG 1 cut(s) 809
HphI GGTGA 2 cut(s) 364, 574
Hpy166II GTNNAC 3 cut(s) 226, 649, 1279
Hpy188I TCNGA 6 cut(s) 221, 244, 273, 485, 748, 1321
Hpy188III TCNNGA 6 cut(s) 10, 344, 616, 1132, 1325, 1339
Hpy8I GTNNAC 3 cut(s) 226, 649, 1279
HpyAV CCTTC 5 cut(s) 45, 425, 935, 1160, 1365
HpyCH4III ACNGT 4 cut(s) 277, 296, 454, 613
HpyCH4IV ACGT 2 cut(s) 210, 1067
HpyCH4V TGCA 6 cut(s) 35, 428, 518, 698, 761, 778
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 35, 41, 174, 515, 587
HpyF3I CTNAG 3 cut(s) 62, 459, 1233
HpySE526I ACGT 2 cut(s) 210, 1067
Hsp92II CATG 8 cut(s) 253, 738, 782, 842, 1094, 1112, 1195, 1252
Ksp22I TGATCA 1 cut(s) 1024
Kzo9I GATC 4 cut(s) 74, 436, 1024, 1321
LguI GCTCTTC 1 cut(s) 382
LmnI GCTCC 3 cut(s) 410, 500, 688
Lsp1109I GCAGC 3 cut(s) 47, 647, 713
LweI GCATC 1 cut(s) 1219
MabI ACCWGGT 1 cut(s) 1182
MaeI CTAG 5 cut(s) 264, 414, 570, 632, 1008
MaeII ACGT 2 cut(s) 210, 1067
MaeIII GTNAC 2 cut(s) 796, 1070
MalI GATC 4 cut(s) 76, 438, 1026, 1323
MboI GATC 4 cut(s) 74, 436, 1024, 1321
MfeI CAATTG 3 cut(s) 504, 513, 1020
MhlI GDGCHC 1 cut(s) 394
MluCI AATT 7 cut(s) 92, 440, 504, 513, 620, 641, 1020
MlyI GAGTC 2 cut(s) 596, 1122
MmeI TCCRAC 1 cut(s) 278
Mph1103I ATGCAT 1 cut(s) 780
MseI TTAA 3 cut(s) 521, 813, 1298
MspI CCGG 1 cut(s) 809
MspR9I CCNGG 6 cut(s) 329, 335, 477, 663, 1084, 1184
MunI CAATTG 3 cut(s) 504, 513, 1020
MvaI CCWGG 6 cut(s) 329, 335, 477, 663, 1084, 1184
MwoI GCNNNNNNNGC 6 cut(s) 32, 35, 41, 174, 515, 587
NdeII GATC 4 cut(s) 74, 436, 1024, 1321
NlaIII CATG 8 cut(s) 253, 738, 782, 842, 1094, 1112, 1195, 1252
NlaIV GGNNCC 1 cut(s) 1005
NmuCI GTSAC 1 cut(s) 1070
NsiI ATGCAT 1 cut(s) 780
NspI RCATGY 3 cut(s) 738, 1094, 1195
PaeI GCATGC 1 cut(s) 1195
PceI AGGCCT 1 cut(s) 1354
PciI ACATGT 2 cut(s) 734, 1090
PciSI GCTCTTC 1 cut(s) 382
PcsI WCGNNNNNNNCGW 1 cut(s) 391
PfeI GAWTC 5 cut(s) 280, 480, 884, 1145, 1153
PkrI GCNGC 5 cut(s) 28, 37, 637, 728, 731
Ple19I CGATCG 1 cut(s) 77
PleI GAGTC 2 cut(s) 596, 1122
PpsI GAGTC 2 cut(s) 596, 1122
PscI ACATGT 2 cut(s) 734, 1090
Psp6I CCWGG 6 cut(s) 327, 333, 475, 661, 1082, 1182
PspGI CCWGG 6 cut(s) 327, 333, 475, 661, 1082, 1182
PspN4I GGNNCC 1 cut(s) 1005
PspPI GGNCC 2 cut(s) 312, 331
PvuI CGATCG 1 cut(s) 77
RsaI GTAC 4 cut(s) 164, 227, 287, 709
RsaNI GTAC 4 cut(s) 163, 226, 286, 708
SapI GCTCTTC 1 cut(s) 382
SaqAI TTAA 3 cut(s) 521, 813, 1298
SatI GCNGC 5 cut(s) 27, 36, 636, 727, 730
Sau3AI GATC 4 cut(s) 74, 436, 1024, 1321
Sau96I GGNCC 2 cut(s) 312, 331
ScaI AGTACT 1 cut(s) 287
SchI GAGTC 2 cut(s) 596, 1122
ScrFI CCNGG 6 cut(s) 329, 335, 477, 663, 1084, 1184
SduI GDGCHC 1 cut(s) 394
SexAI ACCWGGT 1 cut(s) 1182
SfaNI GCATC 1 cut(s) 1219
SmlI CTYRAG 2 cut(s) 344, 1130
SmoI CTYRAG 2 cut(s) 344, 1130
SphI GCATGC 1 cut(s) 1195
Sse9I AATT 7 cut(s) 92, 440, 504, 513, 620, 641, 1020
SseBI AGGCCT 1 cut(s) 1354
SsiI CCGC 4 cut(s) 27, 67, 729, 1195
SspI AATATT 2 cut(s) 870, 1031
SspMI CTAG 5 cut(s) 264, 414, 570, 632, 1008
StuI AGGCCT 1 cut(s) 1354
StyD4I CCNGG 6 cut(s) 327, 333, 475, 661, 1082, 1182
StyI CCWWGG 3 cut(s) 188, 1007, 1214
TaaI ACNGT 4 cut(s) 277, 296, 454, 613
TaiI ACGT 2 cut(s) 213, 1070
TaqI TCGA 3 cut(s) 73, 385, 1126
TaqII GACCGA 1 cut(s) 146
TasI AATT 7 cut(s) 92, 440, 504, 513, 620, 641, 1020
TatI WGTACW 2 cut(s) 225, 285
TauI GCSGC 2 cut(s) 29, 732
TfiI GAWTC 5 cut(s) 280, 480, 884, 1145, 1153
Tru1I TTAA 3 cut(s) 521, 813, 1298
Tru9I TTAA 3 cut(s) 521, 813, 1298
TscAI CASTG 2 cut(s) 543, 795
TseFI GTSAC 1 cut(s) 1070
TseI GCWGC 3 cut(s) 35, 635, 726
Tsp45I GTSAC 1 cut(s) 1070
TspDTI ATGAA 3 cut(s) 372, 954, 1190
TspGWI ACGGA 2 cut(s) 481, 909
TspRI CASTG 2 cut(s) 543, 795
XagI CCTNNNNNAGG 1 cut(s) 332
XapI RAATTY 1 cut(s) 620
XceI RCATGY 3 cut(s) 738, 1094, 1195
XcmI CCANNNNNNNNNTGG 2 cut(s) 325, 1090
XmaJI CCTAGG 1 cut(s) 1007
XmiI GTMKAC 1 cut(s) 1278
XspI CTAG 5 cut(s) 264, 414, 570, 632, 1008
ZrmI AGTACT 1 cut(s) 287
Zsp2I ATGCAT 1 cut(s) 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.