Rh5CG378900

A G-specific adenine DNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0011052
Physical Location & Seq
Reverse (-)
49405207 .. 49410491
5285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG378900.1

Sequence Viewer

Length: 783 bp
ATGTTATCGTCACTTTTTGATTTTCATGTTTATGTGAATAGGAAACTAGCAGCCCAATTAGTTGACCCTTTCCAGCCTGGGGACTTCAATCAGGCTCTAATGGAGCTTGGTGCAACTGTATGTACGCCAACAAGTCCAAGCTGCGGCACATGTCCTGTATCTGACCAATGTTGTGCATTGTCAATATCTAGGCATGATAGTTCAGTGGTGGTTACAGATTATCCAGTTAAGGTGGTAAAGACCAAACAAAGACATGATTTCTCTGCTGTATGTGTTGTGGAAATATTGGGAGATGAGGAATCATCAAACGGACACCAAATCAACAATAAGTTTCTTCTGGTAAAAAGGCCTGATGAAGGGTTGCTTGCTGGCCTCTGGGAGTTCCCGTCTGTCTCATTGGCTGGAGAAGTTGGATTGGTTCCTAGGAGAAAGGCAATTGATCAATATTTGAATAAGTATTTCACACTTAACCCCAGAAAGACGTGTGACATAGTTTCCAGGGAACATGTGGGAGAATATGTCCATGTTTTCTCTCATATTCGACTCAAGATGTATGTGGAATCGTTGATTTTACGTGTTGAAGGTGGGATGAATGACCTGGTTAGCAAGCGGGACAACGAAATCGTGCCTTGGAAATGTGTGGATGCCGAGATTCTTTCAAACATGGGATTGACATCTGGTGTAAGAAAGGTATACACTATGGTTCAAAAGTTAAAGCAAGAAAAGTCGTCTTCTGATCCTGCCTTGGACATCAAGAAGAGAAAAAGGCCTTCAATAAAGTAG

Protein Analysis

260

Amino Acids

29.11

Weight (kDa)

8.55

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX_4 PF14815 108 - 232 1.1e-12 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12740 AT4G12740 AT4G12740
fragaria_vesca FvH4_3g28500 FvH4_3g28500 FvH4_3g28500 FvH4_3g28500
malus_domestica MD15G1141300.v1.1
prunus_persica Prupe.6G214800_v2.0.a1
pyrus_communis pycom15g12760
rosa_chinensis RchiOBHm_Chr5g0052821
rosa_laevigata RLG00000034790
rosa_multiflora Rmu_ssc0000311.1_g000017
rosa_roxburghii Rroxscaffold_1G00028640
rosa_rugosa Rorug05G0269500
rosa_samantha Rh5AG341500 Rh5BG352200 Rh5CG378900 Rh5DG368500
rosa_wichuraiana Rw5G032200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 693
AciI CCGC 2 cut(s) 144, 610
AclWI GGATC 1 cut(s) 731
AfaI GTAC 1 cut(s) 124
AfiI CCNNNNNNNGG 3 cut(s) 79, 143, 356
AflIII ACRYGT 4 cut(s) 149, 482, 505, 574
AgsI TTSAA 6 cut(s) 88, 451, 581, 660, 707, 774
AjiI CACGTC 1 cut(s) 483
AjnI CCWGG 3 cut(s) 76, 497, 597
AluBI AGCT 2 cut(s) 106, 141
AluI AGCT 2 cut(s) 106, 141
Alw26I GTCTC 1 cut(s) 397
AlwI GGATC 1 cut(s) 731
AoxI GGCC 3 cut(s) 347, 370, 767
ApeKI GCWGC 2 cut(s) 50, 141
AspA2I CCTAGG 1 cut(s) 422
AvrII CCTAGG 1 cut(s) 422
BbsI GAAGAC 1 cut(s) 723
BbvI GCAGC 2 cut(s) 62, 128
BciT130I CCWGG 3 cut(s) 78, 499, 599
BclI TGATCA 1 cut(s) 439
BcoDI GTCTC 1 cut(s) 397
BfaI CTAG 3 cut(s) 47, 189, 423
BisI GCNGC 3 cut(s) 51, 142, 145
BlnI CCTAGG 1 cut(s) 422
BlsI GCNGC 3 cut(s) 52, 143, 146
Bme1390I CCNGG 3 cut(s) 78, 499, 599
BmgBI CACGTC 1 cut(s) 483
BmiI GGNNCC 1 cut(s) 420
BmrFI CCNGG 3 cut(s) 78, 499, 599
BmsI GCATC 1 cut(s) 634
BpiI GAAGAC 1 cut(s) 723
BpmI CTGGAG 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 530
BsaAI YACGTR 1 cut(s) 575
BsaBI GATNNNNATC 1 cut(s) 673
BsaJI CCNNGG 5 cut(s) 77, 422, 498, 629, 744
BsaXI ACNNNNNCTCC 4 cut(s) 371, 401, 504, 534
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 143, 356
Bse1I ACTGG 1 cut(s) 224
Bse8I GATNNNNATC 1 cut(s) 673
BseBI CCWGG 3 cut(s) 78, 499, 599
BseDI CCNNGG 5 cut(s) 77, 422, 498, 629, 744
BseGI GGATG 2 cut(s) 594, 649
BseJI GATNNNNATC 1 cut(s) 673
BseLI CCNNNNNNNGG 3 cut(s) 79, 143, 356
BseNI ACTGG 1 cut(s) 224
BseXI GCAGC 2 cut(s) 62, 128
BshFI GGCC 3 cut(s) 349, 372, 769
BslFI GGGAC 2 cut(s) 95, 626
BslI CCNNNNNNNGG 3 cut(s) 79, 143, 356
BsmAI GTCTC 1 cut(s) 397
BsmFI GGGAC 2 cut(s) 95, 626
BsnI GGCC 3 cut(s) 349, 372, 769
Bsp143I GATC 2 cut(s) 439, 736
BspACI CCGC 2 cut(s) 144, 610
BspANI GGCC 3 cut(s) 349, 372, 769
BspLI GGNNCC 1 cut(s) 420
BspPI GGATC 1 cut(s) 731
BsrI ACTGG 1 cut(s) 224
BssECI CCNNGG 5 cut(s) 77, 422, 498, 629, 744
BssMI GATC 2 cut(s) 439, 736
BssNAI GTATAC 1 cut(s) 694
BssT1I CCWWGG 3 cut(s) 422, 629, 744
Bst1107I GTATAC 1 cut(s) 694
Bst2UI CCWGG 3 cut(s) 78, 499, 599
Bst4CI ACNGT 1 cut(s) 118
Bst6I CTCTTC 1 cut(s) 752
BstBAI YACGTR 1 cut(s) 575
BstC8I GCNNGC 3 cut(s) 366, 370, 608
BstENI CCTNNNNNAGG 1 cut(s) 354
BstF5I GGATG 2 cut(s) 594, 649
BstKTI GATC 2 cut(s) 442, 739
BstMAI GTCTC 1 cut(s) 397
BstMBI GATC 2 cut(s) 439, 736
BstNI CCWGG 3 cut(s) 78, 499, 599
BstNSI RCATGY 2 cut(s) 153, 509
BstSCI CCNGG 3 cut(s) 76, 497, 597
BstV1I GCAGC 2 cut(s) 62, 128
BstV2I GAAGAC 1 cut(s) 723
BstZ17I GTATAC 1 cut(s) 694
BsuRI GGCC 3 cut(s) 349, 372, 769
BtrI CACGTC 1 cut(s) 483
BtsCI GGATG 2 cut(s) 594, 649
BtsIMutI CAGTG 1 cut(s) 210
Cac8I GCNNGC 3 cut(s) 366, 370, 608
CsiI ACCWGGT 1 cut(s) 597
Csp6I GTAC 1 cut(s) 123
CviAII CATG 7 cut(s) 26, 150, 194, 254, 506, 524, 664
CviJI RGCY 9 cut(s) 53, 76, 95, 106, 141, 349, 372, 401, 769
CviKI_1 RGCY 9 cut(s) 53, 76, 95, 106, 141, 349, 372, 401, 769
CviQI GTAC 1 cut(s) 123
DpnI GATC 2 cut(s) 441, 738
DpnII GATC 2 cut(s) 439, 736
Eam1104I CTCTTC 1 cut(s) 752
EarI CTCTTC 1 cut(s) 752
Eco130I CCWWGG 3 cut(s) 422, 629, 744
Eco147I AGGCCT 2 cut(s) 349, 769
EcoNI CCTNNNNNAGG 1 cut(s) 354
EcoRII CCWGG 3 cut(s) 76, 497, 597
EcoT14I CCWWGG 3 cut(s) 422, 629, 744
ErhI CCWWGG 3 cut(s) 422, 629, 744
FaeI CATG 7 cut(s) 29, 153, 197, 257, 509, 527, 667
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FaqI GGGAC 2 cut(s) 95, 626
FatI CATG 7 cut(s) 25, 149, 193, 253, 505, 523, 663
FauI CCCGC 1 cut(s) 603
FbaI TGATCA 1 cut(s) 439
FblI GTMKAC 1 cut(s) 693
Fnu4HI GCNGC 3 cut(s) 51, 142, 145
FokI GGATG 2 cut(s) 601, 656
Fsp4HI GCNGC 3 cut(s) 51, 142, 145
FspBI CTAG 3 cut(s) 47, 189, 423
GluI GCNGC 3 cut(s) 51, 142, 145
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 3 cut(s) 349, 372, 769
Hin1II CATG 7 cut(s) 29, 153, 197, 257, 509, 527, 667
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HinfI GANTC 4 cut(s) 299, 543, 560, 652
Hpy166II GTNNAC 2 cut(s) 64, 694
Hpy188I TCNGA 2 cut(s) 163, 736
Hpy188III TCNNGA 2 cut(s) 547, 754
Hpy8I GTNNAC 2 cut(s) 64, 694
HpyAV CCTTC 3 cut(s) 350, 575, 780
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4IV ACGT 2 cut(s) 482, 574
HpyCH4V TGCA 2 cut(s) 113, 176
HpySE526I ACGT 2 cut(s) 482, 574
Hsp92II CATG 7 cut(s) 29, 153, 197, 257, 509, 527, 667
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 2 cut(s) 439, 736
LmnI GCTCC 1 cut(s) 103
Lsp1109I GCAGC 2 cut(s) 62, 128
LweI GCATC 1 cut(s) 634
MabI ACCWGGT 1 cut(s) 597
MaeI CTAG 3 cut(s) 47, 189, 423
MaeII ACGT 2 cut(s) 482, 574
MaeIII GTNAC 3 cut(s) 9, 211, 485
MalI GATC 2 cut(s) 441, 738
MboI GATC 2 cut(s) 439, 736
MboII GAAGA 3 cut(s) 326, 723, 769
MfeI CAATTG 1 cut(s) 435
MluCI AATT 2 cut(s) 56, 435
MlyI GAGTC 1 cut(s) 537
MmeI TCCRAC 1 cut(s) 391
MnlI CCTC 2 cut(s) 289, 383
MseI TTAA 3 cut(s) 228, 468, 713
MslI CAYNNNNRTG 1 cut(s) 30
MspR9I CCNGG 3 cut(s) 78, 499, 599
MunI CAATTG 1 cut(s) 435
MvaI CCWGG 3 cut(s) 78, 499, 599
NdeII GATC 2 cut(s) 439, 736
NlaIII CATG 7 cut(s) 29, 153, 197, 257, 509, 527, 667
NlaIV GGNNCC 1 cut(s) 420
NmeAIII GCCGAG 1 cut(s) 673
NmuCI GTSAC 2 cut(s) 9, 485
NspI RCATGY 2 cut(s) 153, 509
PceI AGGCCT 2 cut(s) 349, 769
PciI ACATGT 2 cut(s) 149, 505
PfeI GAWTC 3 cut(s) 299, 560, 652
PkrI GCNGC 3 cut(s) 52, 143, 146
PleI GAGTC 1 cut(s) 537
PpsI GAGTC 1 cut(s) 537
Ppu21I YACGTR 1 cut(s) 575
PscI ACATGT 2 cut(s) 149, 505
Psp6I CCWGG 3 cut(s) 76, 497, 597
PspGI CCWGG 3 cut(s) 76, 497, 597
PspN4I GGNNCC 1 cut(s) 420
RsaI GTAC 1 cut(s) 124
RsaNI GTAC 1 cut(s) 123
RseI CAYNNNNRTG 1 cut(s) 30
SaqAI TTAA 3 cut(s) 228, 468, 713
SatI GCNGC 3 cut(s) 51, 142, 145
Sau3AI GATC 2 cut(s) 439, 736
SchI GAGTC 1 cut(s) 537
ScrFI CCNGG 3 cut(s) 78, 499, 599
SetI ASST 8 cut(s) 108, 143, 234, 485, 577, 586, 600, 693
SexAI ACCWGGT 1 cut(s) 597
SfaNI GCATC 1 cut(s) 634
SmiMI CAYNNNNRTG 1 cut(s) 30
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
Sse9I AATT 2 cut(s) 56, 435
SseBI AGGCCT 2 cut(s) 349, 769
SsiI CCGC 2 cut(s) 144, 610
SspI AATATT 2 cut(s) 285, 446
SspMI CTAG 3 cut(s) 47, 189, 423
StuI AGGCCT 2 cut(s) 349, 769
StyD4I CCNGG 3 cut(s) 76, 497, 597
StyI CCWWGG 3 cut(s) 422, 629, 744
TaaI ACNGT 1 cut(s) 118
TaiI ACGT 2 cut(s) 485, 577
TaqI TCGA 1 cut(s) 541
TasI AATT 2 cut(s) 56, 435
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 3 cut(s) 299, 560, 652
Tru1I TTAA 3 cut(s) 228, 468, 713
Tru9I TTAA 3 cut(s) 228, 468, 713
TscAI CASTG 1 cut(s) 210
TseFI GTSAC 2 cut(s) 9, 485
TseI GCWGC 2 cut(s) 50, 141
Tsp45I GTSAC 2 cut(s) 9, 485
TspDTI ATGAA 3 cut(s) 14, 369, 605
TspGWI ACGGA 1 cut(s) 324
TspRI CASTG 1 cut(s) 210
XagI CCTNNNNNAGG 1 cut(s) 354
XceI RCATGY 2 cut(s) 153, 509
XcmI CCANNNNNNNNNTGG 1 cut(s) 505
XmaJI CCTAGG 1 cut(s) 422
XmiI GTMKAC 1 cut(s) 693
XspI CTAG 3 cut(s) 47, 189, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.