RchiOBHm_Chr6g0251221

Protein phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0018206
Physical Location & Seq
Reverse (-)
6525425 .. 6527381
1957 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22522

Sequence Viewer

Length: 1323 bp
ATGGTAGACTTATTATTACAAGTTCTTCTATGGAGTTTCTATCTCCTCTCTCTTGTTCTACACCTTTTTGAAGACCTGAAAAAATTAGCCTTTTCCATAGCTCTAACTCTCAACTCTTCTCCTTCAGCATCTCCTTTGTCACGGTATCTCTCTTGGGTTGGGTCTCTTTTTGCGACAATTCAGAGCATTTACCATCACACCCAAGGCCAGATCAGGTCGAATGTAAAAAGGCGGGTTTTTGAGCAGCATGGTGAAGAAAACTATGAAGCAAAGAATTTGGCACATACCATAAGCTGCCAAGACAGTAACAGTAAGATGAAAGAAAAGAATGAAGATAAAAAAGGTATGCATGGAATACTGCATAGTGCACAAAAGAAGTCTGGTCTTTCGGGTAATAGAGGGAGTGCTATCAATAGTAAGTTGCGAGTGAGGCCTGCGAAGCTGTTTGTGCCCGATTTTTGTCCAAGGCAGGAGTTCATTGAAATGGGACTAAAGAAGTTGAAAGAGGACAAAAAGGAATTTGAGGTGGAAGGGAGGGATTTCTGCTTGGCAAGTAAGAAAGGCGCTAGAAGGGAGTTCATGGAAGATGGGCACAGAGAAATGGTTGATATAATGGGAGATCCCAAGCAGGCATTTTTTGCTGTGATCGATGGGCACGGAGGTTGTGCAGCTGCTGATTATGTAGCTGAAAATTTAGGGAAGAACATTATGAAAGAGCTTGAACATGTTGGAGAGGATCAAGAACATGAATTGCAGCAGGCTATTCGCCAAGGCTACTCAGTCACAGACAAGGGCTTCCTTAGTCAGGGTGTGAGCAGTGGAGCATGTGCAGCCAGTGTGGTGTTGAAGGATGGAGTGCTACATGTAGCAAATTTAGGAGATTGCAGAGTAGTTTTGAGTAACAAAGGAGTAGCACATGTATTAACAAAAGACCACCGTTTGAGTAGTGAAGATGAGCGTCTCCGAATCGAAAACACCGGTGGCTTTGTGCAACATTGCCGCAACGGAGTTTGGAGACTTCAAGGCTCACTTGCAGTTTCTAGAGCAATTGGGGATCAACATTTGAAAGATTGGGTCATTTCCGAACCAGAAACTAAAACGATTCCTCTAACTTCAGATTGCGAGTTCTTGCTAATGGCTTCGGATGGCTTGTGGGACAAGGTAGATAATCAAGAGGCAGTGAATGTGGTTTTAAAAGAGAAGGACTCACTGAAGTCTTGCAAGAAGCTTGTGAATATGTCTTCAAGCAGAGGGAGCATGGATGACATAACTGTTATGGTGATCAACCTTCGAAGCTTTTTGGCAGCTGATCATCTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

440

Amino Acids

49.07

Weight (kDa)

6.99

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 185 - 423 1.8e-54 Protein phosphatase 2C
PP2C_2 PF13672 207 - 395 6.1e-06 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018206)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0251221
rosa_laevigata RLG00000015473
rosa_multiflora Rmu_sc0004077.1_g000002
rosa_roxburghii Rroxscaffold_7G00216910
rosa_rugosa Rorug05G0500800
rosa_samantha Rh6AG010200 Rh6BG001800 Rh6CG007200 Rh6DG007700
rosa_wichuraiana Rw6G001000 Rw6G001110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 6
AciI CCGC 2 cut(s) 232, 1000
AclWI GGATC 3 cut(s) 614, 744, 1062
AcsI RAATTY 4 cut(s) 274, 518, 691, 871
AcuI CTGAAG 4 cut(s) 108, 1098, 1232, 1301
AfiI CCNNNNNNNGG 1 cut(s) 805
AflIII ACRYGT 3 cut(s) 724, 862, 916
AgeI ACCGGT 1 cut(s) 977
AgsI TTSAA 8 cut(s) 71, 482, 502, 722, 847, 1022, 1066, 1245
AluBI AGCT 9 cut(s) 101, 294, 442, 671, 686, 718, 1228, 1296, 1307
AluI AGCT 9 cut(s) 101, 294, 442, 671, 686, 718, 1228, 1296, 1307
Alw21I GWGCWC 1 cut(s) 370
Alw26I GTCTC 3 cut(s) 168, 965, 1009
Alw44I GTGCAC 1 cut(s) 366
AlwI GGATC 3 cut(s) 614, 744, 1062
AlwNI CAGNNNCTG 1 cut(s) 674
AoxI GGCC 2 cut(s) 205, 431
ApaLI GTGCAC 1 cut(s) 366
ApeKI GCWGC 7 cut(s) 244, 294, 668, 671, 754, 830, 1304
ApoI RAATTY 4 cut(s) 274, 518, 691, 871
AsiGI ACCGGT 1 cut(s) 977
AspLEI GCGC 1 cut(s) 566
AsuHPI GGTGA 2 cut(s) 263, 1291
AsuII TTCGAA 1 cut(s) 1291
BaeGI GKGCMC 4 cut(s) 370, 453, 594, 657
BbsI GAAGAC 2 cut(s) 78, 1233
Bbv12I GWGCWC 1 cut(s) 370
BbvI GCAGC 7 cut(s) 256, 281, 658, 680, 766, 842, 1316
BccI CCATC 5 cut(s) 201, 581, 644, 845, 1139
BcgI CGANNNNNNTGC 2 cut(s) 746, 780
BclI TGATCA 2 cut(s) 1281, 1309
BcoDI GTCTC 3 cut(s) 168, 965, 1009
BfaI CTAG 2 cut(s) 567, 1041
BfoI RGCGCY 1 cut(s) 567
BisI GCNGC 8 cut(s) 245, 295, 669, 672, 755, 831, 1000, 1305
BlsI GCNGC 8 cut(s) 246, 296, 670, 673, 756, 832, 1001, 1306
BmsI GCATC 1 cut(s) 137
BpiI GAAGAC 2 cut(s) 78, 1233
BplI GAGNNNNNCTC 2 cut(s) 1190, 1222
Bpu14I TTCGAA 1 cut(s) 1291
Bsa29I ATCGAT 1 cut(s) 648
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 3 cut(s) 202, 464, 769
BsaWI WCCGGW 1 cut(s) 977
Bsc4I CCNNNNNNNGG 1 cut(s) 805
Bse118I RCCGGY 1 cut(s) 977
Bse1I ACTGG 1 cut(s) 834
Bse3DI GCAATG 1 cut(s) 994
BseCI ATCGAT 1 cut(s) 648
BseDI CCNNGG 3 cut(s) 202, 464, 769
BseGI GGATG 3 cut(s) 856, 1150, 1267
BseLI CCNNNNNNNGG 1 cut(s) 805
BseMI GCAATG 1 cut(s) 994
BseMII CTCAG 1 cut(s) 792
BseNI ACTGG 1 cut(s) 834
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 4 cut(s) 370, 453, 594, 657
BseXI GCAGC 7 cut(s) 256, 281, 658, 680, 766, 842, 1316
BsgI GTGCAG 2 cut(s) 687, 849
BshFI GGCC 2 cut(s) 207, 433
BshTI ACCGGT 1 cut(s) 977
BshVI ATCGAT 1 cut(s) 648
BsiHKAI GWGCWC 1 cut(s) 370
BsiSI CCGG 1 cut(s) 978
BslFI GGGAC 2 cut(s) 501, 1169
BslI CCNNNNNNNGG 1 cut(s) 805
BsmAI GTCTC 3 cut(s) 168, 965, 1009
BsmBI CGTCTC 1 cut(s) 965
BsmFI GGGAC 2 cut(s) 501, 1169
BsnI GGCC 2 cut(s) 207, 433
Bso31I GGTCTC 1 cut(s) 168
Bsp119I TTCGAA 1 cut(s) 1291
Bsp1286I GDGCHC 4 cut(s) 370, 453, 594, 657
Bsp143I GATC 7 cut(s) 210, 619, 645, 736, 1054, 1281, 1309
BspACI CCGC 2 cut(s) 232, 1000
BspANI GGCC 2 cut(s) 207, 433
BspCNI CTCAG 1 cut(s) 791
BspDI ATCGAT 1 cut(s) 648
BspPI GGATC 3 cut(s) 614, 744, 1062
BspT104I TTCGAA 1 cut(s) 1291
BspTNI GGTCTC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 994
BsrFI RCCGGY 1 cut(s) 977
BsrI ACTGG 1 cut(s) 834
BssAI RCCGGY 1 cut(s) 977
BssECI CCNNGG 3 cut(s) 202, 464, 769
BssMI GATC 7 cut(s) 210, 619, 645, 736, 1054, 1281, 1309
BssT1I CCWWGG 3 cut(s) 202, 464, 769
Bst4CI ACNGT 5 cut(s) 144, 305, 311, 938, 1273
Bst6I CTCTTC 1 cut(s) 121
BstAPI GCANNNNNTGC 1 cut(s) 638
BstBI TTCGAA 1 cut(s) 1291
BstC8I GCNNGC 3 cut(s) 435, 630, 759
BstDEI CTNAG 2 cut(s) 778, 800
BstENI CCTNNNNNAGG 1 cut(s) 803
BstF5I GGATG 3 cut(s) 856, 1150, 1267
BstH2I RGCGCY 1 cut(s) 567
BstHHI GCGC 1 cut(s) 566
BstKTI GATC 7 cut(s) 213, 622, 648, 739, 1057, 1284, 1312
BstMAI GTCTC 3 cut(s) 168, 965, 1009
BstMBI GATC 7 cut(s) 210, 619, 645, 736, 1054, 1281, 1309
BstMWI GCNNNNNNNGC 6 cut(s) 430, 439, 448, 638, 830, 1254
BstNSI RCATGY 4 cut(s) 728, 828, 866, 920
BstSLI GKGCMC 4 cut(s) 370, 453, 594, 657
BstV1I GCAGC 7 cut(s) 256, 281, 658, 680, 766, 842, 1316
BstV2I GAAGAC 2 cut(s) 78, 1233
BstX2I RGATCY 1 cut(s) 619
BstYI RGATCY 1 cut(s) 619
Bsu15I ATCGAT 1 cut(s) 648
BsuRI GGCC 2 cut(s) 207, 433
BsuTUI ATCGAT 1 cut(s) 648
BtsCI GGATG 3 cut(s) 856, 1150, 1267
BtsI GCAGTG 2 cut(s) 823, 1185
BtsIMutI CAGTG 4 cut(s) 823, 841, 1185, 1208
Cac8I GCNNGC 3 cut(s) 435, 630, 759
CaiI CAGNNNCTG 1 cut(s) 674
CfoI GCGC 1 cut(s) 566
Cfr10I RCCGGY 1 cut(s) 977
ClaI ATCGAT 1 cut(s) 648
CseI GACGC 1 cut(s) 947
CspAI ACCGGT 1 cut(s) 977
CviAII CATG 9 cut(s) 248, 350, 580, 725, 746, 825, 863, 917, 1258
DdeI CTNAG 2 cut(s) 778, 800
DpnI GATC 7 cut(s) 212, 621, 647, 738, 1056, 1283, 1311
DpnII GATC 7 cut(s) 210, 619, 645, 736, 1054, 1281, 1309
DraI TTTAAA 1 cut(s) 1194
Eam1104I CTCTTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 121
Eco130I CCWWGG 3 cut(s) 202, 464, 769
Eco147I AGGCCT 1 cut(s) 433
Eco31I GGTCTC 1 cut(s) 168
Eco57I CTGAAG 4 cut(s) 108, 1098, 1232, 1301
EcoNI CCTNNNNNAGG 1 cut(s) 803
EcoT14I CCWWGG 3 cut(s) 202, 464, 769
EcoT22I ATGCAT 1 cut(s) 351
ErhI CCWWGG 3 cut(s) 202, 464, 769
Esp3I CGTCTC 1 cut(s) 965
FaeI CATG 9 cut(s) 251, 353, 583, 728, 749, 828, 866, 920, 1261
FalI AAGNNNNNCTT 2 cut(s) 1014, 1046
FaqI GGGAC 2 cut(s) 501, 1169
FatI CATG 9 cut(s) 247, 349, 579, 724, 745, 824, 862, 916, 1257
FauI CCCGC 1 cut(s) 225
FbaI TGATCA 2 cut(s) 1281, 1309
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 8 cut(s) 245, 295, 669, 672, 755, 831, 1000, 1305
FokI GGATG 3 cut(s) 863, 1157, 1274
Fsp4HI GCNGC 8 cut(s) 245, 295, 669, 672, 755, 831, 1000, 1305
FspBI CTAG 2 cut(s) 567, 1041
GlaI GCGC 1 cut(s) 565
GluI GCNGC 8 cut(s) 245, 295, 669, 672, 755, 831, 1000, 1305
HaeII RGCGCY 1 cut(s) 567
HaeIII GGCC 2 cut(s) 207, 433
HapII CCGG 1 cut(s) 978
HgaI GACGC 1 cut(s) 947
HhaI GCGC 1 cut(s) 566
Hin1II CATG 9 cut(s) 251, 353, 583, 728, 749, 828, 866, 920, 1261
Hin6I GCGC 1 cut(s) 564
HinP1I GCGC 1 cut(s) 564
HindIII AAGCTT 2 cut(s) 1226, 1294
HinfI GANTC 3 cut(s) 966, 1102, 1205
HpaII CCGG 1 cut(s) 978
HphI GGTGA 2 cut(s) 263, 1291
Hpy166II GTNNAC 2 cut(s) 7, 368
Hpy188I TCNGA 5 cut(s) 183, 965, 1084, 1117, 1144
Hpy188III TCNNGA 3 cut(s) 740, 1041, 1172
Hpy8I GTNNAC 2 cut(s) 7, 368
HpyAV CCTTC 6 cut(s) 132, 524, 564, 841, 1195, 1298
HpyCH4III ACNGT 5 cut(s) 144, 305, 311, 938, 1273
HpyF10VI GCNNNNNNNGC 6 cut(s) 430, 439, 448, 638, 830, 1254
HpyF3I CTNAG 2 cut(s) 778, 800
Hsp92II CATG 9 cut(s) 251, 353, 583, 728, 749, 828, 866, 920, 1261
HspAI GCGC 1 cut(s) 564
Ksp22I TGATCA 2 cut(s) 1281, 1309
Kzo9I GATC 7 cut(s) 210, 619, 645, 736, 1054, 1281, 1309
LmnI GCTCC 2 cut(s) 821, 1254
Lsp1109I GCAGC 7 cut(s) 256, 281, 658, 680, 766, 842, 1316
LweI GCATC 1 cut(s) 137
MaeI CTAG 2 cut(s) 567, 1041
MaeIII GTNAC 4 cut(s) 138, 305, 781, 899
MalI GATC 7 cut(s) 212, 621, 647, 738, 1056, 1283, 1311
MboI GATC 7 cut(s) 210, 619, 645, 736, 1054, 1281, 1309
MfeI CAATTG 1 cut(s) 1047
MflI RGATCY 1 cut(s) 619
MhlI GDGCHC 4 cut(s) 370, 453, 594, 657
MluCI AATT 8 cut(s) 83, 177, 274, 518, 691, 749, 871, 1047
MlyI GAGTC 1 cut(s) 1199
MmeI TCCRAC 1 cut(s) 709
Mph1103I ATGCAT 1 cut(s) 351
MseI TTAA 2 cut(s) 923, 1193
MslI CAYNNNNRTG 1 cut(s) 482
MspA1I CMGCKG 2 cut(s) 671, 1307
MspI CCGG 1 cut(s) 978
MunI CAATTG 1 cut(s) 1047
MwoI GCNNNNNNNGC 6 cut(s) 430, 439, 448, 638, 830, 1254
NdeII GATC 7 cut(s) 210, 619, 645, 736, 1054, 1281, 1309
NlaIII CATG 9 cut(s) 251, 353, 583, 728, 749, 828, 866, 920, 1261
NmuCI GTSAC 2 cut(s) 138, 781
NsiI ATGCAT 1 cut(s) 351
NspI RCATGY 4 cut(s) 728, 828, 866, 920
NspV TTCGAA 1 cut(s) 1291
PceI AGGCCT 1 cut(s) 433
PciI ACATGT 3 cut(s) 724, 862, 916
PfeI GAWTC 2 cut(s) 966, 1102
PinAI ACCGGT 1 cut(s) 977
PkrI GCNGC 8 cut(s) 246, 296, 670, 673, 756, 832, 1001, 1306
PleI GAGTC 1 cut(s) 1199
PpsI GAGTC 1 cut(s) 1199
PscI ACATGT 3 cut(s) 724, 862, 916
PstNI CAGNNNCTG 1 cut(s) 674
PsuI RGATCY 1 cut(s) 619
PvuII CAGCTG 2 cut(s) 671, 1307
RseI CAYNNNNRTG 1 cut(s) 482
SaqAI TTAA 2 cut(s) 923, 1193
SatI GCNGC 8 cut(s) 245, 295, 669, 672, 755, 831, 1000, 1305
Sau3AI GATC 7 cut(s) 210, 619, 645, 736, 1054, 1281, 1309
SchI GAGTC 1 cut(s) 1199
SduI GDGCHC 4 cut(s) 370, 453, 594, 657
SfaNI GCATC 1 cut(s) 137
SfuI TTCGAA 1 cut(s) 1291
SgrAI CRCCGGYG 1 cut(s) 977
SmiMI CAYNNNNRTG 1 cut(s) 482
Sse9I AATT 8 cut(s) 83, 177, 274, 518, 691, 749, 871, 1047
SseBI AGGCCT 1 cut(s) 433
SsiI CCGC 2 cut(s) 232, 1000
SspMI CTAG 2 cut(s) 567, 1041
StuI AGGCCT 1 cut(s) 433
StyI CCWWGG 3 cut(s) 202, 464, 769
TaaI ACNGT 5 cut(s) 144, 305, 311, 938, 1273
TaqI TCGA 4 cut(s) 218, 648, 969, 1291
TasI AATT 8 cut(s) 83, 177, 274, 518, 691, 749, 871, 1047
TauI GCSGC 1 cut(s) 1002
TfiI GAWTC 2 cut(s) 966, 1102
Tru1I TTAA 2 cut(s) 923, 1193
Tru9I TTAA 2 cut(s) 923, 1193
TscAI CASTG 4 cut(s) 823, 841, 1185, 1215
TseFI GTSAC 2 cut(s) 138, 781
TseI GCWGC 7 cut(s) 244, 294, 668, 671, 754, 830, 1304
Tsp45I GTSAC 2 cut(s) 138, 781
TspDTI ATGAA 7 cut(s) 279, 332, 345, 466, 568, 725, 762
TspGWI ACGGA 2 cut(s) 672, 1020
TspRI CASTG 4 cut(s) 823, 841, 1185, 1215
VneI GTGCAC 1 cut(s) 366
XagI CCTNNNNNAGG 1 cut(s) 803
XapI RAATTY 4 cut(s) 274, 518, 691, 871
XbaI TCTAGA 1 cut(s) 1040
XceI RCATGY 4 cut(s) 728, 828, 866, 920
XmiI GTMKAC 1 cut(s) 6
XspI CTAG 2 cut(s) 567, 1041
Zsp2I ATGCAT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.