RLG00000015473

Protein phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0018206
Physical Location & Seq
Forward (+)
66573042 .. 66575002
1961 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015473

Sequence Viewer

Length: 1320 bp
ATGGTAGACTTATTATTACAACTTCTTCTATGGAGTTTCTATCTCCTCTCTCTTGTTCTACACCTTTTTGAAGACCTGAAAAAAGTCTTTTCCATAGCTCTAACTCTCAACTCTTCTCCTTCAGTATCTCCTTTGTCACGGTATCTCTCTTGGGTTGGGTCTCTTTTTGCGAGAATTCAGAGCATTTACCATTACACCCAAGGCCAGATCAGGTCGAATGTAAAAAAGCGGGTTTTGGAGCAGCATGGTGAAGAAAACTATGAAGCAAAGAATTTGGCACATATCATAAGCTGCCAAGACAGTAACAGTAAGATGAAAGAAAAGAATGAAGATCACAGAGGTATGCATGAAGTACTGCATAGTTCACAAAAGAAGTCTGGTCTTTCGGGTAATAGAGGGAGTGCTATCAATAGTAAGTTGCGAATGAGGCCTGCGAAGCTGTTTGTGCCGGATTTTTGTCCAAGGCAGGAGTTCATTGAAATGGGACTAAAGAAGTTGAAAGAGGACAAAAAGGAATTTGAGGTGGAAGGGAGGGATTTCTGCTTGGCAAGTAAGAAAGGTGCTAGAAGGGAGTTCATGGAAGATGGGCACAGAGAAATGGTTGATATAATGGGAGATCCCAAGCAGGCATTTTTTGCTGTGATCGATGGGCACGGAGGTTGTGCAGCTGCTGATTATGTAGCTGAAAATTTAGGGAAGAACATTATGAAAGAGCTTGAACATGTTGGAGAGGATCAAGAACATGAATTGCAGCAGGCTGTTCGCCAAGGCTACTCAGTCACAGACAAGGGCTTCCTTAGTCAGGGTGTGAGCAGCGGAGCATGTGCAGCCAGTGTGGTGTTGAAGGATGGAGTGCTACATGTAGCAAATGTAGGAGATTGTAGAGTAGTTTTGAGTAACAAAGGAGTAGCACATGTATTAACAAAAGACCACCGTTTGAGTAGTGAAGATGAGCGTCTCCGAATCGAAAACACCGGTGGCTTTGTGCAACATTTCCGCAACGGAGTTTGGAGACTTCAAGGCTCACTTGCAGTTTCTAGAGCAATTGGGGATCAACATTTGAAAGATTGGGTCATTTCTGAACCAGAAACTAAAACGATTCCTCTAACATCAGATTGCGAGTTCTTGATAATGGCTTCGGATGGCTTGTGGGACAAGGTAGATGATCAAGAGGCAGTGAATGTGGTTTTAAAAGAGAAGGATTCACTGAAGTCTTGCAAGAAGCTTGTGAATATGTCTTCAAGCAGAGGGAGCATGGATGACATTACTGTTATGGTGATCAACCTTCGAAGCTTTTCGGCAGCTGATCATCTTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

440

Amino Acids

49.16

Weight (kDa)

6.64

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 184 - 422 2.5e-55 Protein phosphatase 2C
PP2C_2 PF13672 206 - 394 9.2e-06 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018206)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0251221
rosa_laevigata RLG00000015473
rosa_multiflora Rmu_sc0004077.1_g000002
rosa_roxburghii Rroxscaffold_7G00216910
rosa_rugosa Rorug05G0500800
rosa_samantha Rh6AG010200 Rh6BG001800 Rh6CG007200 Rh6DG007700
rosa_wichuraiana Rw6G001000 Rw6G001110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 6
AciI CCGC 3 cut(s) 229, 816, 997
AclWI GGATC 3 cut(s) 611, 741, 1059
AcsI RAATTY 4 cut(s) 174, 271, 515, 688
AcuI CTGAAG 2 cut(s) 105, 1229
AfaI GTAC 1 cut(s) 354
AfiI CCNNNNNNNGG 1 cut(s) 802
AflIII ACRYGT 3 cut(s) 721, 859, 913
AgeI ACCGGT 1 cut(s) 974
AgsI TTSAA 8 cut(s) 71, 479, 499, 719, 844, 1019, 1063, 1242
AluBI AGCT 9 cut(s) 98, 291, 439, 668, 683, 715, 1225, 1293, 1304
AluI AGCT 9 cut(s) 98, 291, 439, 668, 683, 715, 1225, 1293, 1304
Alw26I GTCTC 3 cut(s) 165, 962, 1006
AlwI GGATC 3 cut(s) 611, 741, 1059
AlwNI CAGNNNCTG 1 cut(s) 671
AoxI GGCC 2 cut(s) 202, 428
ApeKI GCWGC 8 cut(s) 241, 291, 665, 668, 751, 813, 827, 1301
ApoI RAATTY 4 cut(s) 174, 271, 515, 688
AsiGI ACCGGT 1 cut(s) 974
Asp700I GAANNNNTTC 1 cut(s) 1294
AsuHPI GGTGA 2 cut(s) 260, 1288
AsuII TTCGAA 1 cut(s) 1288
BaeGI GKGCMC 2 cut(s) 591, 654
BbsI GAAGAC 2 cut(s) 78, 1230
BbvI GCAGC 8 cut(s) 253, 278, 655, 677, 763, 825, 839, 1313
BccI CCATC 4 cut(s) 578, 641, 842, 1136
BcgI CGANNNNNNTGC 2 cut(s) 743, 777
BclI TGATCA 3 cut(s) 1165, 1278, 1306
BcoDI GTCTC 3 cut(s) 165, 962, 1006
BfaI CTAG 2 cut(s) 564, 1038
BisI GCNGC 8 cut(s) 242, 292, 666, 669, 752, 814, 828, 1302
BlsI GCNGC 8 cut(s) 243, 293, 667, 670, 753, 815, 829, 1303
BmcAI AGTACT 1 cut(s) 354
BpiI GAAGAC 2 cut(s) 78, 1230
Bpu14I TTCGAA 1 cut(s) 1288
Bsa29I ATCGAT 1 cut(s) 645
BsaI GGTCTC 1 cut(s) 165
BsaJI CCNNGG 3 cut(s) 199, 461, 766
BsaWI WCCGGW 1 cut(s) 974
Bsc4I CCNNNNNNNGG 1 cut(s) 802
Bse118I RCCGGY 1 cut(s) 974
Bse1I ACTGG 1 cut(s) 831
BseCI ATCGAT 1 cut(s) 645
BseDI CCNNGG 3 cut(s) 199, 461, 766
BseGI GGATG 3 cut(s) 853, 1147, 1264
BseLI CCNNNNNNNGG 1 cut(s) 802
BseMII CTCAG 1 cut(s) 789
BseNI ACTGG 1 cut(s) 831
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 2 cut(s) 591, 654
BseXI GCAGC 8 cut(s) 253, 278, 655, 677, 763, 825, 839, 1313
BsgI GTGCAG 2 cut(s) 684, 846
BshFI GGCC 2 cut(s) 204, 430
BshTI ACCGGT 1 cut(s) 974
BshVI ATCGAT 1 cut(s) 645
BsiSI CCGG 2 cut(s) 449, 975
BslFI GGGAC 2 cut(s) 498, 1166
BslI CCNNNNNNNGG 1 cut(s) 802
BsmAI GTCTC 3 cut(s) 165, 962, 1006
BsmBI CGTCTC 1 cut(s) 962
BsmFI GGGAC 2 cut(s) 498, 1166
BsnI GGCC 2 cut(s) 204, 430
Bso31I GGTCTC 1 cut(s) 165
Bsp119I TTCGAA 1 cut(s) 1288
Bsp1286I GDGCHC 2 cut(s) 591, 654
Bsp143I GATC 9 cut(s) 207, 331, 616, 642, 733, 1051, 1165, 1278, 1306
BspACI CCGC 3 cut(s) 229, 816, 997
BspANI GGCC 2 cut(s) 204, 430
BspCNI CTCAG 1 cut(s) 788
BspDI ATCGAT 1 cut(s) 645
BspPI GGATC 3 cut(s) 611, 741, 1059
BspT104I TTCGAA 1 cut(s) 1288
BspTNI GGTCTC 1 cut(s) 165
BsrFI RCCGGY 1 cut(s) 974
BsrI ACTGG 1 cut(s) 831
BssAI RCCGGY 1 cut(s) 974
BssECI CCNNGG 3 cut(s) 199, 461, 766
BssMI GATC 9 cut(s) 207, 331, 616, 642, 733, 1051, 1165, 1278, 1306
BssT1I CCWWGG 3 cut(s) 199, 461, 766
Bst4CI ACNGT 5 cut(s) 141, 302, 308, 935, 1270
Bst6I CTCTTC 1 cut(s) 118
BstAPI GCANNNNNTGC 1 cut(s) 635
BstBI TTCGAA 1 cut(s) 1288
BstC8I GCNNGC 3 cut(s) 432, 627, 756
BstDEI CTNAG 2 cut(s) 775, 797
BstENI CCTNNNNNAGG 1 cut(s) 800
BstF5I GGATG 3 cut(s) 853, 1147, 1264
BstKTI GATC 9 cut(s) 210, 334, 619, 645, 736, 1054, 1168, 1281, 1309
BstMAI GTCTC 3 cut(s) 165, 962, 1006
BstMBI GATC 9 cut(s) 207, 331, 616, 642, 733, 1051, 1165, 1278, 1306
BstMWI GCNNNNNNNGC 6 cut(s) 427, 436, 445, 635, 827, 1251
BstNSI RCATGY 4 cut(s) 725, 825, 863, 917
BstSLI GKGCMC 2 cut(s) 591, 654
BstV1I GCAGC 8 cut(s) 253, 278, 655, 677, 763, 825, 839, 1313
BstV2I GAAGAC 2 cut(s) 78, 1230
BstX2I RGATCY 1 cut(s) 616
BstYI RGATCY 1 cut(s) 616
Bsu15I ATCGAT 1 cut(s) 645
BsuRI GGCC 2 cut(s) 204, 430
BsuTUI ATCGAT 1 cut(s) 645
BtsCI GGATG 3 cut(s) 853, 1147, 1264
BtsI GCAGTG 1 cut(s) 1182
BtsIMutI CAGTG 3 cut(s) 838, 1182, 1205
Cac8I GCNNGC 3 cut(s) 432, 627, 756
CaiI CAGNNNCTG 1 cut(s) 671
Cfr10I RCCGGY 1 cut(s) 974
ClaI ATCGAT 1 cut(s) 645
CseI GACGC 1 cut(s) 944
Csp6I GTAC 1 cut(s) 353
CspAI ACCGGT 1 cut(s) 974
CviAII CATG 9 cut(s) 245, 347, 577, 722, 743, 822, 860, 914, 1255
CviQI GTAC 1 cut(s) 353
DdeI CTNAG 2 cut(s) 775, 797
DpnI GATC 9 cut(s) 209, 333, 618, 644, 735, 1053, 1167, 1280, 1308
DpnII GATC 9 cut(s) 207, 331, 616, 642, 733, 1051, 1165, 1278, 1306
DraI TTTAAA 1 cut(s) 1191
Eam1104I CTCTTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 118
Eco130I CCWWGG 3 cut(s) 199, 461, 766
Eco147I AGGCCT 1 cut(s) 430
Eco31I GGTCTC 1 cut(s) 165
Eco57I CTGAAG 2 cut(s) 105, 1229
EcoNI CCTNNNNNAGG 1 cut(s) 800
EcoRI GAATTC 1 cut(s) 174
EcoT14I CCWWGG 3 cut(s) 199, 461, 766
EcoT22I ATGCAT 1 cut(s) 348
ErhI CCWWGG 3 cut(s) 199, 461, 766
Esp3I CGTCTC 1 cut(s) 962
FaeI CATG 9 cut(s) 248, 350, 580, 725, 746, 825, 863, 917, 1258
FalI AAGNNNNNCTT 2 cut(s) 1011, 1043
FaqI GGGAC 2 cut(s) 498, 1166
FatI CATG 9 cut(s) 244, 346, 576, 721, 742, 821, 859, 913, 1254
FauI CCCGC 1 cut(s) 222
FbaI TGATCA 3 cut(s) 1165, 1278, 1306
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 8 cut(s) 242, 292, 666, 669, 752, 814, 828, 1302
FokI GGATG 3 cut(s) 860, 1154, 1271
Fsp4HI GCNGC 8 cut(s) 242, 292, 666, 669, 752, 814, 828, 1302
FspBI CTAG 2 cut(s) 564, 1038
GluI GCNGC 8 cut(s) 242, 292, 666, 669, 752, 814, 828, 1302
HaeIII GGCC 2 cut(s) 204, 430
HapII CCGG 2 cut(s) 449, 975
HgaI GACGC 1 cut(s) 944
Hin1II CATG 9 cut(s) 248, 350, 580, 725, 746, 825, 863, 917, 1258
HindIII AAGCTT 2 cut(s) 1223, 1291
HinfI GANTC 3 cut(s) 963, 1099, 1202
HpaII CCGG 2 cut(s) 449, 975
HphI GGTGA 2 cut(s) 260, 1288
Hpy166II GTNNAC 2 cut(s) 7, 365
Hpy188I TCNGA 5 cut(s) 180, 962, 1081, 1114, 1141
Hpy188III TCNNGA 4 cut(s) 737, 1038, 1126, 1169
Hpy8I GTNNAC 2 cut(s) 7, 365
HpyAV CCTTC 6 cut(s) 129, 521, 561, 838, 1192, 1295
HpyCH4III ACNGT 5 cut(s) 141, 302, 308, 935, 1270
HpyCH4V TGCA 8 cut(s) 346, 358, 665, 751, 827, 988, 1031, 1218
HpyF10VI GCNNNNNNNGC 6 cut(s) 427, 436, 445, 635, 827, 1251
HpyF3I CTNAG 2 cut(s) 775, 797
Hsp92II CATG 9 cut(s) 248, 350, 580, 725, 746, 825, 863, 917, 1258
Ksp22I TGATCA 3 cut(s) 1165, 1278, 1306
Kzo9I GATC 9 cut(s) 207, 331, 616, 642, 733, 1051, 1165, 1278, 1306
LmnI GCTCC 3 cut(s) 238, 818, 1251
Lsp1109I GCAGC 8 cut(s) 253, 278, 655, 677, 763, 825, 839, 1313
MaeI CTAG 2 cut(s) 564, 1038
MaeIII GTNAC 4 cut(s) 135, 302, 778, 896
MalI GATC 9 cut(s) 209, 333, 618, 644, 735, 1053, 1167, 1280, 1308
MboI GATC 9 cut(s) 207, 331, 616, 642, 733, 1051, 1165, 1278, 1306
MfeI CAATTG 1 cut(s) 1044
MflI RGATCY 1 cut(s) 616
MhlI GDGCHC 2 cut(s) 591, 654
MluCI AATT 6 cut(s) 174, 271, 515, 688, 746, 1044
MmeI TCCRAC 1 cut(s) 706
Mph1103I ATGCAT 1 cut(s) 348
MroXI GAANNNNTTC 1 cut(s) 1294
MseI TTAA 2 cut(s) 920, 1190
MslI CAYNNNNRTG 1 cut(s) 479
MspA1I CMGCKG 3 cut(s) 668, 816, 1304
MspI CCGG 2 cut(s) 449, 975
MunI CAATTG 1 cut(s) 1044
MwoI GCNNNNNNNGC 6 cut(s) 427, 436, 445, 635, 827, 1251
NdeII GATC 9 cut(s) 207, 331, 616, 642, 733, 1051, 1165, 1278, 1306
NlaIII CATG 9 cut(s) 248, 350, 580, 725, 746, 825, 863, 917, 1258
NmuCI GTSAC 2 cut(s) 135, 778
NsiI ATGCAT 1 cut(s) 348
NspI RCATGY 4 cut(s) 725, 825, 863, 917
NspV TTCGAA 1 cut(s) 1288
PceI AGGCCT 1 cut(s) 430
PciI ACATGT 3 cut(s) 721, 859, 913
PdmI GAANNNNTTC 1 cut(s) 1294
PfeI GAWTC 3 cut(s) 963, 1099, 1202
PinAI ACCGGT 1 cut(s) 974
PkrI GCNGC 8 cut(s) 243, 293, 667, 670, 753, 815, 829, 1303
PscI ACATGT 3 cut(s) 721, 859, 913
PstNI CAGNNNCTG 1 cut(s) 671
PsuI RGATCY 1 cut(s) 616
PvuII CAGCTG 2 cut(s) 668, 1304
RsaI GTAC 1 cut(s) 354
RsaNI GTAC 1 cut(s) 353
RseI CAYNNNNRTG 1 cut(s) 479
SaqAI TTAA 2 cut(s) 920, 1190
SatI GCNGC 8 cut(s) 242, 292, 666, 669, 752, 814, 828, 1302
Sau3AI GATC 9 cut(s) 207, 331, 616, 642, 733, 1051, 1165, 1278, 1306
ScaI AGTACT 1 cut(s) 354
SduI GDGCHC 2 cut(s) 591, 654
SfuI TTCGAA 1 cut(s) 1288
SgrAI CRCCGGYG 1 cut(s) 974
SmiMI CAYNNNNRTG 1 cut(s) 479
Sse9I AATT 6 cut(s) 174, 271, 515, 688, 746, 1044
SseBI AGGCCT 1 cut(s) 430
SsiI CCGC 3 cut(s) 229, 816, 997
SspMI CTAG 2 cut(s) 564, 1038
StuI AGGCCT 1 cut(s) 430
StyI CCWWGG 3 cut(s) 199, 461, 766
TaaI ACNGT 5 cut(s) 141, 302, 308, 935, 1270
TaqI TCGA 4 cut(s) 215, 645, 966, 1288
TasI AATT 6 cut(s) 174, 271, 515, 688, 746, 1044
TatI WGTACW 1 cut(s) 352
TfiI GAWTC 3 cut(s) 963, 1099, 1202
Tru1I TTAA 2 cut(s) 920, 1190
Tru9I TTAA 2 cut(s) 920, 1190
TscAI CASTG 3 cut(s) 838, 1182, 1212
TseFI GTSAC 2 cut(s) 135, 778
TseI GCWGC 8 cut(s) 241, 291, 665, 668, 751, 813, 827, 1301
Tsp45I GTSAC 2 cut(s) 135, 778
TspDTI ATGAA 8 cut(s) 276, 329, 342, 363, 463, 565, 722, 759
TspGWI ACGGA 3 cut(s) 669, 1017, 1305
TspRI CASTG 3 cut(s) 838, 1182, 1212
XagI CCTNNNNNAGG 1 cut(s) 800
XapI RAATTY 4 cut(s) 174, 271, 515, 688
XbaI TCTAGA 1 cut(s) 1037
XceI RCATGY 4 cut(s) 725, 825, 863, 917
XmiI GTMKAC 1 cut(s) 6
XmnI GAANNNNTTC 1 cut(s) 1294
XspI CTAG 2 cut(s) 564, 1038
ZrmI AGTACT 1 cut(s) 354
Zsp2I ATGCAT 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.