Rh6AG010200

Protein phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0018206
Physical Location & Seq
Reverse (-)
1119494 .. 1121851
2358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG010200.1

Sequence Viewer

Length: 1317 bp
ATGGTAGACTTATTATTACAAGTTCTTCTATGGAGTTTCTATCTCCTCTCTCTTGTTCTACACCTTTTTGAAGACCTGAAAAAATTAGCCTTTTCCATAGCTCTAACTCTCAACTCTTCTCCTTCAGCATCTCCTTTGTCACGGTATCTCTCTTGGGTTGGGTCTCTTTTTGCGACAATTCAGAGCATTTACCATCACACCCAAGGCCAGATCAGGTCGAATGTAAAAGGGTGGGTTTTTGAGCAGCATGGTGAAGAAAACTATGAAGCAAAGAATTTGGCACATACCATAAGCTGCCAAGACAGTAACAGTAAGATGAAAGAAAAGAATGAAAAAGGTATGCATGGAATACTGCATAGTTCACAAAAGAAGTCTGGTCTTTCGGGTAATAGAGGGAGTGCTATCAATAGTAAGTTGCGAGTGAGGCCTGCGAAGCTGTTTGTGCCGGATTTTTGTCCAAGGCAGGAGTTCATTGAAATGGGACTAAAGAAGTTGAAAGAGGACAAAAAGGAATTTGAGGTGGAAGGGAGGGATTTCTGCTTGGCAAGTAAGAAAGGTGCTAGAAGGGAGTTCATGGAAGATGGGCACAGAGAAATGGTTGATATAATGGGAGATCCCAGGCAGGCATTTTTTGCTGTGATCGATGGGCACGGAGGTTGTGCAGCTGCTGATTATGTAGCTGAAAATTTAGGGAAGAACATTATGAAAGAGCTTGAACATGTTGGAGAGGATCAAGAACATGAATTGCAGCAGGCTATTCGCCAAGGCTACTCAGTCACAGACAAGGGCTTCCTTAGTCAGGGTGTGAGCAGTGGAGCATGTGCAGCCAGTGTGGTGTTGAAGGATGGAGCGCTACATGTAGCAAATTTAGGAGATTGTAGAGTAGTTTTGAGTAACAAAGGAGTAGCACATGTATTAACAAAAGACCACCGTTTGAGTAGTGAAGATGAGCGTCTCCGAATCGAAAACACCGGTGGCTTTGTGCAACATTGCCGCAACGGAGTTTGGAGACTTCAAGGCTCACTTGCAGTTTCTAGAGCAATTGGGGATCAACATTTGAAAGATTGGGTCATTTCCGAACCAGAAACTAAAACGATTCCTCTAACTTCAGATTGCGAGTTCTTGCTAATGGCTTCGGATGGCTTGTGGGACAAGGTAGATGATCAAGAGGCAGTGAATGTGGTTTTAAAAGAGAAGGACTCACTGAAGTCTTGCAAGAAGCTTGTGAATATGTCTTCAAGCAGAGGGAGCATGGATGACATAACTGTTATGGTGATCAACCTTCGAAGCTTTTTGGCAGCTGATCATCTTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

438

Amino Acids

48.74

Weight (kDa)

6.5

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 183 - 421 1.3e-54 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018206)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0251221
rosa_laevigata RLG00000015473
rosa_multiflora Rmu_sc0004077.1_g000002
rosa_roxburghii Rroxscaffold_7G00216910
rosa_rugosa Rorug05G0500800
rosa_samantha Rh6AG010200 Rh6BG001800 Rh6CG007200 Rh6DG007700
rosa_wichuraiana Rw6G001000 Rw6G001110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 6
AciI CCGC 1 cut(s) 994
AclWI GGATC 3 cut(s) 608, 738, 1056
AcsI RAATTY 4 cut(s) 274, 512, 685, 865
AcuI CTGAAG 3 cut(s) 108, 1092, 1226
AfeI AGCGCT 1 cut(s) 852
AfiI CCNNNNNNNGG 1 cut(s) 799
AflIII ACRYGT 3 cut(s) 718, 856, 910
AgeI ACCGGT 1 cut(s) 971
AgsI TTSAA 8 cut(s) 71, 476, 496, 716, 841, 1016, 1060, 1239
AjnI CCWGG 1 cut(s) 617
AluBI AGCT 9 cut(s) 101, 294, 436, 665, 680, 712, 1222, 1290, 1301
AluI AGCT 9 cut(s) 101, 294, 436, 665, 680, 712, 1222, 1290, 1301
Alw26I GTCTC 3 cut(s) 168, 959, 1003
AlwI GGATC 3 cut(s) 608, 738, 1056
AlwNI CAGNNNCTG 1 cut(s) 668
Aor51HI AGCGCT 1 cut(s) 852
AoxI GGCC 2 cut(s) 205, 425
ApeKI GCWGC 7 cut(s) 244, 294, 662, 665, 748, 824, 1298
ApoI RAATTY 4 cut(s) 274, 512, 685, 865
AsiGI ACCGGT 1 cut(s) 971
AspLEI GCGC 1 cut(s) 853
AsuHPI GGTGA 2 cut(s) 263, 1285
AsuII TTCGAA 1 cut(s) 1285
BaeGI GKGCMC 2 cut(s) 588, 651
BbsI GAAGAC 2 cut(s) 78, 1227
BbvI GCAGC 7 cut(s) 256, 281, 652, 674, 760, 836, 1310
BccI CCATC 5 cut(s) 201, 575, 638, 839, 1133
BcgI CGANNNNNNTGC 2 cut(s) 740, 774
BciT130I CCWGG 1 cut(s) 619
BclI TGATCA 3 cut(s) 1162, 1275, 1303
BcoDI GTCTC 3 cut(s) 168, 959, 1003
BfaI CTAG 2 cut(s) 561, 1035
BfoI RGCGCY 1 cut(s) 854
BisI GCNGC 8 cut(s) 245, 295, 663, 666, 749, 825, 994, 1299
BlsI GCNGC 8 cut(s) 246, 296, 664, 667, 750, 826, 995, 1300
Bme1390I CCNGG 1 cut(s) 619
BmrFI CCNGG 1 cut(s) 619
BmsI GCATC 1 cut(s) 137
BpiI GAAGAC 2 cut(s) 78, 1227
BplI GAGNNNNNCTC 2 cut(s) 1184, 1216
Bpu14I TTCGAA 1 cut(s) 1285
Bsa29I ATCGAT 1 cut(s) 642
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 4 cut(s) 202, 458, 617, 763
BsaWI WCCGGW 1 cut(s) 971
Bsc4I CCNNNNNNNGG 1 cut(s) 799
Bse118I RCCGGY 1 cut(s) 971
Bse1I ACTGG 1 cut(s) 828
Bse3DI GCAATG 1 cut(s) 988
BseBI CCWGG 1 cut(s) 619
BseCI ATCGAT 1 cut(s) 642
BseDI CCNNGG 4 cut(s) 202, 458, 617, 763
BseGI GGATG 3 cut(s) 850, 1144, 1261
BseLI CCNNNNNNNGG 1 cut(s) 799
BseMI GCAATG 1 cut(s) 988
BseMII CTCAG 1 cut(s) 786
BseNI ACTGG 1 cut(s) 828
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 2 cut(s) 588, 651
BseXI GCAGC 7 cut(s) 256, 281, 652, 674, 760, 836, 1310
BsgI GTGCAG 2 cut(s) 681, 843
BshFI GGCC 2 cut(s) 207, 427
BshTI ACCGGT 1 cut(s) 971
BshVI ATCGAT 1 cut(s) 642
BsiSI CCGG 2 cut(s) 446, 972
BslFI GGGAC 2 cut(s) 495, 1163
BslI CCNNNNNNNGG 1 cut(s) 799
BsmAI GTCTC 3 cut(s) 168, 959, 1003
BsmBI CGTCTC 1 cut(s) 959
BsmFI GGGAC 2 cut(s) 495, 1163
BsnI GGCC 2 cut(s) 207, 427
Bso31I GGTCTC 1 cut(s) 168
Bsp119I TTCGAA 1 cut(s) 1285
Bsp1286I GDGCHC 2 cut(s) 588, 651
Bsp143I GATC 8 cut(s) 210, 613, 639, 730, 1048, 1162, 1275, 1303
BspACI CCGC 1 cut(s) 994
BspANI GGCC 2 cut(s) 207, 427
BspCNI CTCAG 1 cut(s) 785
BspDI ATCGAT 1 cut(s) 642
BspPI GGATC 3 cut(s) 608, 738, 1056
BspT104I TTCGAA 1 cut(s) 1285
BspTNI GGTCTC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 988
BsrFI RCCGGY 1 cut(s) 971
BsrI ACTGG 1 cut(s) 828
BssAI RCCGGY 1 cut(s) 971
BssECI CCNNGG 4 cut(s) 202, 458, 617, 763
BssMI GATC 8 cut(s) 210, 613, 639, 730, 1048, 1162, 1275, 1303
BssT1I CCWWGG 3 cut(s) 202, 458, 763
Bst2UI CCWGG 1 cut(s) 619
Bst4CI ACNGT 5 cut(s) 144, 305, 311, 932, 1267
Bst6I CTCTTC 1 cut(s) 121
BstAPI GCANNNNNTGC 1 cut(s) 632
BstBI TTCGAA 1 cut(s) 1285
BstC8I GCNNGC 3 cut(s) 429, 624, 753
BstDEI CTNAG 2 cut(s) 772, 794
BstENI CCTNNNNNAGG 1 cut(s) 797
BstF5I GGATG 3 cut(s) 850, 1144, 1261
BstH2I RGCGCY 1 cut(s) 854
BstHHI GCGC 1 cut(s) 853
BstKTI GATC 8 cut(s) 213, 616, 642, 733, 1051, 1165, 1278, 1306
BstMAI GTCTC 3 cut(s) 168, 959, 1003
BstMBI GATC 8 cut(s) 210, 613, 639, 730, 1048, 1162, 1275, 1303
BstMWI GCNNNNNNNGC 6 cut(s) 424, 433, 442, 632, 824, 1248
BstNI CCWGG 1 cut(s) 619
BstNSI RCATGY 4 cut(s) 722, 822, 860, 914
BstSCI CCNGG 1 cut(s) 617
BstSLI GKGCMC 2 cut(s) 588, 651
BstV1I GCAGC 7 cut(s) 256, 281, 652, 674, 760, 836, 1310
BstV2I GAAGAC 2 cut(s) 78, 1227
BstX2I RGATCY 1 cut(s) 613
BstYI RGATCY 1 cut(s) 613
Bsu15I ATCGAT 1 cut(s) 642
BsuRI GGCC 2 cut(s) 207, 427
BsuTUI ATCGAT 1 cut(s) 642
BtsCI GGATG 3 cut(s) 850, 1144, 1261
BtsI GCAGTG 2 cut(s) 817, 1179
BtsIMutI CAGTG 4 cut(s) 817, 835, 1179, 1202
Cac8I GCNNGC 3 cut(s) 429, 624, 753
CaiI CAGNNNCTG 1 cut(s) 668
CfoI GCGC 1 cut(s) 853
Cfr10I RCCGGY 1 cut(s) 971
ClaI ATCGAT 1 cut(s) 642
CseI GACGC 1 cut(s) 941
CspAI ACCGGT 1 cut(s) 971
CviAII CATG 9 cut(s) 248, 344, 574, 719, 740, 819, 857, 911, 1252
DdeI CTNAG 2 cut(s) 772, 794
DpnI GATC 8 cut(s) 212, 615, 641, 732, 1050, 1164, 1277, 1305
DpnII GATC 8 cut(s) 210, 613, 639, 730, 1048, 1162, 1275, 1303
DraI TTTAAA 1 cut(s) 1188
Eam1104I CTCTTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 121
Eco130I CCWWGG 3 cut(s) 202, 458, 763
Eco147I AGGCCT 1 cut(s) 427
Eco31I GGTCTC 1 cut(s) 168
Eco47III AGCGCT 1 cut(s) 852
Eco57I CTGAAG 3 cut(s) 108, 1092, 1226
EcoNI CCTNNNNNAGG 1 cut(s) 797
EcoRII CCWGG 1 cut(s) 617
EcoT14I CCWWGG 3 cut(s) 202, 458, 763
EcoT22I ATGCAT 1 cut(s) 345
ErhI CCWWGG 3 cut(s) 202, 458, 763
Esp3I CGTCTC 1 cut(s) 959
FaeI CATG 9 cut(s) 251, 347, 577, 722, 743, 822, 860, 914, 1255
FalI AAGNNNNNCTT 2 cut(s) 1008, 1040
FaqI GGGAC 2 cut(s) 495, 1163
FatI CATG 9 cut(s) 247, 343, 573, 718, 739, 818, 856, 910, 1251
FbaI TGATCA 3 cut(s) 1162, 1275, 1303
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 8 cut(s) 245, 295, 663, 666, 749, 825, 994, 1299
FokI GGATG 3 cut(s) 857, 1151, 1268
Fsp4HI GCNGC 8 cut(s) 245, 295, 663, 666, 749, 825, 994, 1299
FspBI CTAG 2 cut(s) 561, 1035
GlaI GCGC 1 cut(s) 852
GluI GCNGC 8 cut(s) 245, 295, 663, 666, 749, 825, 994, 1299
HaeII RGCGCY 1 cut(s) 854
HaeIII GGCC 2 cut(s) 207, 427
HapII CCGG 2 cut(s) 446, 972
HgaI GACGC 1 cut(s) 941
HhaI GCGC 1 cut(s) 853
Hin1II CATG 9 cut(s) 251, 347, 577, 722, 743, 822, 860, 914, 1255
Hin6I GCGC 1 cut(s) 851
HinP1I GCGC 1 cut(s) 851
HindIII AAGCTT 2 cut(s) 1220, 1288
HinfI GANTC 3 cut(s) 960, 1096, 1199
HpaII CCGG 2 cut(s) 446, 972
HphI GGTGA 2 cut(s) 263, 1285
Hpy166II GTNNAC 2 cut(s) 7, 362
Hpy188I TCNGA 5 cut(s) 183, 959, 1078, 1111, 1138
Hpy188III TCNNGA 3 cut(s) 734, 1035, 1166
Hpy8I GTNNAC 2 cut(s) 7, 362
HpyAV CCTTC 6 cut(s) 132, 518, 558, 835, 1189, 1292
HpyCH4III ACNGT 5 cut(s) 144, 305, 311, 932, 1267
HpyCH4V TGCA 8 cut(s) 343, 355, 662, 748, 824, 985, 1028, 1215
HpyF10VI GCNNNNNNNGC 6 cut(s) 424, 433, 442, 632, 824, 1248
HpyF3I CTNAG 2 cut(s) 772, 794
Hsp92II CATG 9 cut(s) 251, 347, 577, 722, 743, 822, 860, 914, 1255
HspAI GCGC 1 cut(s) 851
Ksp22I TGATCA 3 cut(s) 1162, 1275, 1303
Kzo9I GATC 8 cut(s) 210, 613, 639, 730, 1048, 1162, 1275, 1303
LmnI GCTCC 3 cut(s) 815, 848, 1248
Lsp1109I GCAGC 7 cut(s) 256, 281, 652, 674, 760, 836, 1310
LweI GCATC 1 cut(s) 137
MaeI CTAG 2 cut(s) 561, 1035
MaeIII GTNAC 4 cut(s) 138, 305, 775, 893
MalI GATC 8 cut(s) 212, 615, 641, 732, 1050, 1164, 1277, 1305
MboI GATC 8 cut(s) 210, 613, 639, 730, 1048, 1162, 1275, 1303
MboII GAAGA 9 cut(s) 17, 83, 108, 266, 590, 706, 956, 1227, 1301
MfeI CAATTG 1 cut(s) 1041
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 2 cut(s) 588, 651
MluCI AATT 8 cut(s) 83, 177, 274, 512, 685, 743, 865, 1041
MlyI GAGTC 1 cut(s) 1193
MmeI TCCRAC 1 cut(s) 703
Mph1103I ATGCAT 1 cut(s) 345
MseI TTAA 2 cut(s) 917, 1187
MslI CAYNNNNRTG 1 cut(s) 476
MspA1I CMGCKG 2 cut(s) 665, 1301
MspI CCGG 2 cut(s) 446, 972
MspR9I CCNGG 1 cut(s) 619
MunI CAATTG 1 cut(s) 1041
MvaI CCWGG 1 cut(s) 619
MwoI GCNNNNNNNGC 6 cut(s) 424, 433, 442, 632, 824, 1248
NdeII GATC 8 cut(s) 210, 613, 639, 730, 1048, 1162, 1275, 1303
NlaIII CATG 9 cut(s) 251, 347, 577, 722, 743, 822, 860, 914, 1255
NmuCI GTSAC 2 cut(s) 138, 775
NsiI ATGCAT 1 cut(s) 345
NspI RCATGY 4 cut(s) 722, 822, 860, 914
NspV TTCGAA 1 cut(s) 1285
PceI AGGCCT 1 cut(s) 427
PciI ACATGT 3 cut(s) 718, 856, 910
PfeI GAWTC 2 cut(s) 960, 1096
PinAI ACCGGT 1 cut(s) 971
PkrI GCNGC 8 cut(s) 246, 296, 664, 667, 750, 826, 995, 1300
PleI GAGTC 1 cut(s) 1193
PpsI GAGTC 1 cut(s) 1193
PscI ACATGT 3 cut(s) 718, 856, 910
Psp6I CCWGG 1 cut(s) 617
PspGI CCWGG 1 cut(s) 617
PstNI CAGNNNCTG 1 cut(s) 668
PsuI RGATCY 1 cut(s) 613
PvuII CAGCTG 2 cut(s) 665, 1301
RseI CAYNNNNRTG 1 cut(s) 476
SaqAI TTAA 2 cut(s) 917, 1187
SatI GCNGC 8 cut(s) 245, 295, 663, 666, 749, 825, 994, 1299
Sau3AI GATC 8 cut(s) 210, 613, 639, 730, 1048, 1162, 1275, 1303
SchI GAGTC 1 cut(s) 1193
ScrFI CCNGG 1 cut(s) 619
SduI GDGCHC 2 cut(s) 588, 651
SfaNI GCATC 1 cut(s) 137
SfuI TTCGAA 1 cut(s) 1285
SgrAI CRCCGGYG 1 cut(s) 971
SmiMI CAYNNNNRTG 1 cut(s) 476
Sse9I AATT 8 cut(s) 83, 177, 274, 512, 685, 743, 865, 1041
SseBI AGGCCT 1 cut(s) 427
SsiI CCGC 1 cut(s) 994
SspMI CTAG 2 cut(s) 561, 1035
StuI AGGCCT 1 cut(s) 427
StyD4I CCNGG 1 cut(s) 617
StyI CCWWGG 3 cut(s) 202, 458, 763
TaaI ACNGT 5 cut(s) 144, 305, 311, 932, 1267
TaqI TCGA 4 cut(s) 218, 642, 963, 1285
TasI AATT 8 cut(s) 83, 177, 274, 512, 685, 743, 865, 1041
TauI GCSGC 1 cut(s) 996
TfiI GAWTC 2 cut(s) 960, 1096
Tru1I TTAA 2 cut(s) 917, 1187
Tru9I TTAA 2 cut(s) 917, 1187
TscAI CASTG 4 cut(s) 817, 835, 1179, 1209
TseFI GTSAC 2 cut(s) 138, 775
TseI GCWGC 7 cut(s) 244, 294, 662, 665, 748, 824, 1298
Tsp45I GTSAC 2 cut(s) 138, 775
TspDTI ATGAA 7 cut(s) 279, 332, 345, 460, 562, 719, 756
TspGWI ACGGA 3 cut(s) 666, 1014, 1302
TspRI CASTG 4 cut(s) 817, 835, 1179, 1209
XagI CCTNNNNNAGG 1 cut(s) 797
XapI RAATTY 4 cut(s) 274, 512, 685, 865
XbaI TCTAGA 1 cut(s) 1034
XceI RCATGY 4 cut(s) 722, 822, 860, 914
XmiI GTMKAC 1 cut(s) 6
XspI CTAG 2 cut(s) 561, 1035
Zsp2I ATGCAT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.