RchiOBHm_Chr6g0271641

tRNA-dihydrouridine(47) synthase NAD(P)( )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0020574
Physical Location & Seq
Forward (+)
31534427 .. 31536861
2435 bp
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UTR
Exon/CDS
Intron
PRQ24364

Sequence Viewer

Length: 501 bp
ATGCAGAAACCTGAGGAGATTGAGGGAGAATGTCCTTTTATGGCTATTGATGAGCCATGCCCTTATGGTTTGTCATGTAGGTTCTTGGGTACACATAAAAATGGTGTTGCAGATGGAGATGTGAATGCTCGTAGAAGAAGCTCTGAGATGAATGGATTAAGCAAAAATGTTCAAAAGCTTTTGTGGAAAAATAAAATGAAGTTCACCAAGGCCGATGCCAAACTCAAGGCTCTTGGTCTCATGGGGCATGCCAATTCAAAAATAATTGCCATGTCAAAGGAGAATGGGTCGGTGGTTTCAAAGAATTGTGATGTTACTGATGGAAATGGCTGTAGTGAATTTCCCAGTAACACAGATGATAAAGTGGAGTTGACTGCTGAAATAAAAGAAGAGGGACGTGTTGATGAAAAGTTTCCATCTGATGAAATGGAGTTGTCTGTTGAAGTGAAAGAAGAGGGAGGTGCTAATGAAACATTTCCATCGGACGATTCTAGGCCTTAA

Protein Analysis

166

Amino Acids

18.22

Weight (kDa)

4.93

Isoelectric Point (pI)

52.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH_DUS3L PF25585 11 - 34 1.7e-07 DUS3L CCCH zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020574)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0268431 RchiOBHm_Chr6g0268441 RchiOBHm_Chr6g0271641
rosa_roxburghii Rroxscaffold_7G00199810
rosa_samantha Rh6AG161400 Rh6CG159300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 338
AfaI GTAC 1 cut(s) 91
AflIII ACRYGT 1 cut(s) 397
AgsI TTSAA 4 cut(s) 173, 258, 300, 443
AjiI CACGTC 1 cut(s) 398
AluBI AGCT 2 cut(s) 141, 178
AluI AGCT 2 cut(s) 141, 178
Alw26I GTCTC 1 cut(s) 242
AoxI GGCC 2 cut(s) 210, 494
ApoI RAATTY 1 cut(s) 338
Asp700I GAANNNNTTC 2 cut(s) 411, 474
AsuHPI GGTGA 1 cut(s) 196
AxyI CCTNAGG 1 cut(s) 12
BccI CCATC 4 cut(s) 107, 314, 424, 487
BcoDI GTCTC 1 cut(s) 242
BfaI CTAG 1 cut(s) 492
BfmI CTRYAG 1 cut(s) 331
BmgBI CACGTC 1 cut(s) 398
BmrI ACTGGG 1 cut(s) 339
BmsI GCATC 1 cut(s) 205
BmuI ACTGGG 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 209
BsaI GGTCTC 1 cut(s) 242
BsaJI CCNNGG 1 cut(s) 207
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bse1I ACTGG 1 cut(s) 345
Bse21I CCTNAGG 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 207
BseMII CTCAG 1 cut(s) 135
BseNI ACTGG 1 cut(s) 345
BseRI GAGGAG 1 cut(s) 29
BshFI GGCC 2 cut(s) 212, 496
BslFI GGGAC 1 cut(s) 408
BsmAI GTCTC 1 cut(s) 242
BsmFI GGGAC 1 cut(s) 408
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 2 cut(s) 212, 496
Bso31I GGTCTC 1 cut(s) 242
BspANI GGCC 2 cut(s) 212, 496
BspCNI CTCAG 2 cut(s) 4, 136
BspTNI GGTCTC 1 cut(s) 242
BsrI ACTGG 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 207
Bst6I CTCTTC 2 cut(s) 384, 447
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 2 cut(s) 12, 144
BstMAI GTCTC 1 cut(s) 242
BstNSI RCATGY 1 cut(s) 251
BstSFI CTRYAG 1 cut(s) 331
Bsu36I CCTNAGG 1 cut(s) 12
BsuRI GGCC 2 cut(s) 212, 496
BtrI CACGTC 1 cut(s) 398
Cac8I GCNNGC 1 cut(s) 249
Csp6I GTAC 1 cut(s) 90
CviAII CATG 5 cut(s) 57, 75, 241, 248, 271
CviJI RGCY 8 cut(s) 44, 55, 141, 178, 212, 230, 330, 496
CviKI_1 RGCY 8 cut(s) 44, 55, 141, 178, 212, 230, 330, 496
CviQI GTAC 1 cut(s) 90
DdeI CTNAG 2 cut(s) 12, 144
Eam1104I CTCTTC 2 cut(s) 384, 447
EarI CTCTTC 2 cut(s) 384, 447
Eco130I CCWWGG 1 cut(s) 207
Eco147I AGGCCT 1 cut(s) 496
Eco31I GGTCTC 1 cut(s) 242
Eco81I CCTNAGG 1 cut(s) 12
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 5 cut(s) 60, 78, 244, 251, 274
FaiI YATR 8 cut(s) 41, 58, 66, 76, 96, 242, 249, 272
FaqI GGGAC 1 cut(s) 408
FatI CATG 5 cut(s) 56, 74, 240, 247, 270
FspBI CTAG 1 cut(s) 492
HaeIII GGCC 2 cut(s) 212, 496
Hin1II CATG 5 cut(s) 60, 78, 244, 251, 274
HincII GTYRAC 1 cut(s) 372
HindII GTYRAC 1 cut(s) 372
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 1 cut(s) 488
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 3 cut(s) 92, 204, 372
Hpy188I TCNGA 3 cut(s) 145, 421, 484
Hpy8I GTNNAC 3 cut(s) 92, 204, 372
HpyCH4IV ACGT 1 cut(s) 397
HpyCH4V TGCA 2 cut(s) 4, 110
HpyF3I CTNAG 2 cut(s) 12, 144
HpySE526I ACGT 1 cut(s) 397
Hsp92II CATG 5 cut(s) 60, 78, 244, 251, 274
LpnPI CCDG 2 cut(s) 24, 358
LweI GCATC 1 cut(s) 205
MaeI CTAG 1 cut(s) 492
MaeII ACGT 1 cut(s) 397
MaeIII GTNAC 2 cut(s) 313, 347
MboII GAAGA 3 cut(s) 147, 401, 464
MluCI AATT 4 cut(s) 253, 264, 304, 338
MnlI CCTC 5 cut(s) 7, 16, 385, 448, 452
MroXI GAANNNNTTC 2 cut(s) 411, 474
MseI TTAA 2 cut(s) 158, 499
MslI CAYNNNNRTG 1 cut(s) 99
Mva1269I GAATGC 1 cut(s) 130
NlaIII CATG 5 cut(s) 60, 78, 244, 251, 274
NspI RCATGY 1 cut(s) 251
PaeI GCATGC 1 cut(s) 251
PceI AGGCCT 1 cut(s) 496
PctI GAATGC 1 cut(s) 130
PdmI GAANNNNTTC 2 cut(s) 411, 474
PfeI GAWTC 1 cut(s) 488
RsaI GTAC 1 cut(s) 91
RsaNI GTAC 1 cut(s) 90
RseI CAYNNNNRTG 1 cut(s) 99
SaqAI TTAA 2 cut(s) 158, 499
SetI ASST 6 cut(s) 13, 83, 143, 180, 400, 463
SfaNI GCATC 1 cut(s) 205
SfcI CTRYAG 1 cut(s) 331
SmiMI CAYNNNNRTG 1 cut(s) 99
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
SphI GCATGC 1 cut(s) 251
Sse9I AATT 4 cut(s) 253, 264, 304, 338
SseBI AGGCCT 1 cut(s) 496
SspMI CTAG 1 cut(s) 492
StuI AGGCCT 1 cut(s) 496
StyI CCWWGG 1 cut(s) 207
TaiI ACGT 1 cut(s) 400
TasI AATT 4 cut(s) 253, 264, 304, 338
TfiI GAWTC 1 cut(s) 488
Tru1I TTAA 2 cut(s) 158, 499
Tru9I TTAA 2 cut(s) 158, 499
TspDTI ATGAA 5 cut(s) 164, 212, 420, 438, 483
XapI RAATTY 1 cut(s) 338
XceI RCATGY 1 cut(s) 251
XmnI GAANNNNTTC 2 cut(s) 411, 474
XspI CTAG 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.