Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0017079

Physical Location & Seq

Genomic Coordinates Reverse (-)

25381205 .. 25383850

Gene Structure 2646 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ18616

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2184 bp

ATGCATCGCCGCCTCTCCATTTTCACCCACAAGATTCTCACAAATCCCAATTGTATCTCCACCACCACCAAAGTCAGCTCCTTTAGTCCTAGAACCAACAGGTTAGGTTTACTGCGATTGCTTTCGTATACCCCATTGAATCGTCGCCCTGCTGATCCTGAAGACCCGTCCAATTTGATAAAGGAGGATGGGGTTTCGATTTTGTCCCAAATGTGGATTGAGAATTTTCGAGAACCTGATAGGATTGTGACCAATTTGACCTCTTACATTCGGAGATTCGAGCTCTGGGTGTTGGCTTATCAGAAGGTCTGTGCCGACGATATGGGGGCTTACATGCCCCGAAGTTCGATACAGAGGTCGGCACTTGAGGACTTGTTAGGACTTAGAAATGCTGTTCTTGATGGGAGGTTTAGGTGGGGAGCTAGGCTAGAGTTTTATATTAAGTCACCCAAGGATAAGACCGAGTACGAGTCTTTGTCGAAGAGGAAAATTAAGGCCATTTTGACCACCACGCAGCCGTCCCCGTTTCAAGATAAGTTGGTTCAGGAGGTGTTGTTGATGGTGTTGGAGCCGATATACGAATCTCGGTTCTCGCAGAAGTCGTTTGCATTTAGGCCAGGGAGAAATGCACATACTGCGTTGAGGGTGATTAGGAGGAGTTTTGCAGGGTATTTGTGGTATATAAAGGGGGATTTGAGTACAGTGTTGGATGGGATGAAGGTGGGGTTGGTGATTAATGCTCTAATCAGGGATGTGAGGGATAAGAAGGTAGTTGATTTGATTAAAACGGCATTGGTTACACCGGTGATTACTAGTAATGATGATGGTGTAGTGAAGAAAAAAAAGTCGAGGAAGTATCAGAAGAAGAGGGTGCTGGCAGAGGATGAACCGAAGCCAGACCCTTATTGGTTGGAGACGTTTTTTGGGTTTGCTCCTGAGGAGGCAGAGAAACTTCCTTCTTGGGGGCATTGTGGGATACTCAGTCCCCTTTTGGCTAATATTTGTCTAGATGAATTGGACCGCTGGATGGAGGGAAAGATTAAGGAGTTTTATCATCCGTCAAAGAGTGACGTCATATGGAATTGCCCAGAAGGAGAATCAGAACAGGGAAATACGTCTTGGCCTGAATTTGTGCCAACAAGTGGGCCAGATAAGACCAGAAAGATGGATTATATAAGATATGGGGGTCATATTTTGATTGGTGTTCGAGGACCTCGAGCAGATGCAGCCACATTGAGAAAACAGTTGATTGAGTTCTGTGATCAAAAGTATATGCTCAAGCTAGACAATGAGAGCCTCCCTATAGAACACATAACTAAGGGTATATTGTTTCTTGATCATGTGTTGTGTCGCAGAGTTGTCTACCCAACGCTCCGTTACACTGCTACTGGTGGGAAAATTATTAGTGAGAAGGGTGTAGGCACTCTTTTATCAGTTACAGCAAGCTTGAAACAGTGCATCAAGCAATTTAGGAAACTGAGCTTTCTGAAGGGGGATAGGGATCCTGATCCACAGCCTTGTTTCAGAATGTTCCATGCCACTCAAGCTCATACTAATGCACAAATGAACAAGTTTCTGTCAACAATGGTCGAGTGGTATAGGTATGCAGATAATCGCAAAAAAGTTGTAAACTTTTGTGCTTACATTCTACGGGGTTCACTAGCAAAGCTTTATGCCGCAAAATACAAGCTCCGTTCACGTGCAAAGGTGTACAAGATTGGTGATAGGAATCTGAGTCGTCCTTTGAAGGAGAAAAAGGGGCAGTCACCCGAGTACCATAATTTGTTGAGGATGGGTCTTGTTGAGTCAATTGATGGGCTTCAGTATACCAGGATGTCTCTTGTACCTGAGACTGATTACACCCCTTTCCCAAGTAACTGGAGACCTGAACACGAGAAGGCATTGCTTGAATATATAAGGCTTGATGATCCAAAAACGTTAGAGGAGCAGCGCAGGTGTATCATGGATCAAGGTCTTGTTTCTCCGCAGGATTACATCTCAATGCTTGTTTGGAACTACAAGAGAAGTGCTATTCCGTTGGATCAGTTTACATTTGTCAAAAGTGGCAGCAGTGACATTGAAAGAGATAAGCAATTGCTGCTTGGTTCAAATCAGGATAACCTTGAGAACAGAACTACAGAAGAGGAGGAAAAGGGTGAAGGGTTTTGTGTATCACAATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0000959 GO:0000963 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0007005 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090615 GO:0140053 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Intron_maturas2 RVT_1

Protein Analysis

727

Amino Acids

83.88

Weight (kDa)

9.34

Isoelectric Point (pI)

49.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Intron_maturas2	PF01348	479 - 582	9.7e-08	Type II intron maturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0017079)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46920
fragaria_vesca	FvH4_5g21000
malus_domestica	MD06G1041700.v1.1
prunus_persica	Prupe.5G048500_v2.0.a1
pyrus_communis	pycom06g03520
rosa_chinensis	RchiOBHm_Chr7g0208021
rosa_laevigata	RLG00000003262
rosa_roxburghii	Rroxscaffold_3G00250890
rosa_rugosa	Rorug07G0100900
rosa_samantha	Rh7AG235100 Rh7BG230400 Rh7CG250000 Rh7DG241000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1944
AatII	GACGTC	1 cut(s)	1074
Acc36I	ACCTGC	1 cut(s)	1944
AccB7I	CCANNNNNTGG	1 cut(s)	1142
AccI	GTMKAC	3 cut(s)	128, 1362, 1826
AciI	CCGC	4 cut(s)	10, 1021, 1677, 1985
AclI	AACGTT	1 cut(s)	1937
AclWI	GGATC	7 cut(s)	149, 1496, 1502, 1509, 1922, 1974, 2049
AcsI	RAATTY	2 cut(s)	223, 1127
AcuI	CTGAAG	3 cut(s)	180, 1508, 1805
AcvI	CACGTG	1 cut(s)	1700
AcyI	GRCGYC	1 cut(s)	1071
AfaI	GTAC	5 cut(s)	467, 700, 1712, 1775, 1845
AfiI	CCNNNNNNNGG	4 cut(s)	213, 962, 1027, 1142
AgeI	ACCGGT	1 cut(s)	802
AgsI	TTSAA	7 cut(s)	139, 530, 1450, 1747, 1910, 2081, 2109
AhlI	ACTAGT	1 cut(s)	812
AjnI	CCWGG	2 cut(s)	616, 1829
AjuI	GAANNNNNNNTTGG	4 cut(s)	710, 742, 1102, 1134
AluBI	AGCT	9 cut(s)	78, 283, 422, 1282, 1446, 1482, 1547, 1669, 1690
AluI	AGCT	9 cut(s)	78, 283, 422, 1282, 1446, 1482, 1547, 1669, 1690
Alw21I	GWGCWC	1 cut(s)	285
Alw26I	GTCTC	4 cut(s)	908, 1842, 1844, 1876
AlwI	GGATC	7 cut(s)	149, 1496, 1502, 1509, 1922, 1974, 2049
Ama87I	CYCGRG	2 cut(s)	1215, 1769
AoxI	GGCC	4 cut(s)	495, 614, 1121, 1145
ApeKI	GCWGC	5 cut(s)	514, 1226, 1948, 2067, 2098
ApoI	RAATTY	2 cut(s)	223, 1127
AseI	ATTAAT	1 cut(s)	735
AsiGI	ACCGGT	1 cut(s)	802
AspLEI	GCGC	1 cut(s)	1953
AspS9I	GGNCC	3 cut(s)	1018, 1145, 1211
AsuHPI	GGTGA	8 cut(s)	16, 438, 658, 742, 817, 1733, 1758, 2168
AvaI	CYCGRG	2 cut(s)	1215, 1769
AvaII	GGWCC	2 cut(s)	1018, 1211
AxyI	CCTNAGG	1 cut(s)	936
BaeI	ACNNNNGTAYC	4 cut(s)	341, 374, 1757, 1790
BamHI	GGATCC	1 cut(s)	1501
BanII	GRGCYC	1 cut(s)	285
BauI	CACGAG	1 cut(s)	1892
BbrPI	CACGTG	1 cut(s)	1700
BbsI	GAAGAC	1 cut(s)	168
Bbv12I	GWGCWC	1 cut(s)	285
BbvI	GCAGC	5 cut(s)	526, 1238, 1960, 2079, 2085
BccI	CCATC	9 cut(s)	182, 395, 553, 704, 818, 1021, 1159, 1786, 1808
BceAI	ACGGC	2 cut(s)	502, 804
BciT130I	CCWGG	2 cut(s)	618, 1831
BciVI	GTATCC	1 cut(s)	969
BclI	TGATCA	2 cut(s)	1261, 1336
BcoDI	GTCTC	4 cut(s)	908, 1842, 1844, 1876
BcuI	ACTAGT	1 cut(s)	812
BfaI	CTAG	7 cut(s)	90, 423, 428, 813, 1007, 1283, 1661
BfmI	CTRYAG	2 cut(s)	1302, 2136
BfuAI	ACCTGC	1 cut(s)	1944
BfuI	GTATCC	1 cut(s)	969
BisI	GCNGC	7 cut(s)	10, 515, 1227, 1677, 1949, 2068, 2099
BlsI	GCNGC	7 cut(s)	11, 516, 1228, 1678, 1950, 2069, 2100
Bme1390I	CCNGG	2 cut(s)	618, 1831
Bme18I	GGWCC	2 cut(s)	1018, 1211
BmeT110I	CYCGRG	2 cut(s)	1215, 1769
BmgT120I	GGNCC	3 cut(s)	1018, 1145, 1211
BmiI	GGNNCC	2 cut(s)	570, 1503
BmrFI	CCNGG	2 cut(s)	618, 1831
BmsI	GCATC	3 cut(s)	13, 1213, 1467
BpiI	GAAGAC	1 cut(s)	168
BpmI	CTGGAG	1 cut(s)	1900
BpuEI	CTTGAG	4 cut(s)	386, 1262, 1527, 2144
BsaAI	YACGTR	1 cut(s)	1700
BsaBI	GATNNNNATC	3 cut(s)	1500, 1506, 1728
BsaHI	GRCGYC	1 cut(s)	1071
BsaI	GGTCTC	1 cut(s)	1876
BsaJI	CCNNGG	2 cut(s)	450, 617
BsaWI	WCCGGW	1 cut(s)	802
BsaXI	ACNNNNNCTCC	2 cut(s)	539, 569
Bsc4I	CCNNNNNNNGG	4 cut(s)	213, 962, 1027, 1142
Bse118I	RCCGGY	1 cut(s)	802
Bse1I	ACTGG	2 cut(s)	1393, 1883
Bse21I	CCTNAGG	1 cut(s)	936
Bse3DI	GCAATG	1 cut(s)	1901
Bse8I	GATNNNNATC	3 cut(s)	1500, 1506, 1728
BseBI	CCWGG	2 cut(s)	618, 1831
BseDI	CCNNGG	2 cut(s)	450, 617
BseGI	GGATG	9 cut(s)	193, 715, 720, 757, 889, 1032, 1054, 1797, 1839
BseJI	GATNNNNATC	3 cut(s)	1500, 1506, 1728
BseLI	CCNNNNNNNGG	4 cut(s)	213, 962, 1027, 1142
BseMI	GCAATG	1 cut(s)	1901
BseMII	CTCAG	5 cut(s)	927, 994, 1469, 1724, 1839
BseNI	ACTGG	2 cut(s)	1393, 1883
BseRI	GAGGAG	4 cut(s)	670, 953, 1958, 2159
BseXI	GCAGC	5 cut(s)	526, 1238, 1960, 2079, 2085
BshFI	GGCC	4 cut(s)	497, 616, 1123, 1147
BshTI	ACCGGT	1 cut(s)	802
BsiHKAI	GWGCWC	1 cut(s)	285
BsiHKCI	CYCGRG	2 cut(s)	1215, 1769
BsiSI	CCGG	1 cut(s)	803
BslFI	GGGAC	3 cut(s)	190, 505, 969
BslI	CCNNNNNNNGG	4 cut(s)	213, 962, 1027, 1142
BsmAI	GTCTC	4 cut(s)	908, 1842, 1844, 1876
BsmBI	CGTCTC	1 cut(s)	908
BsmFI	GGGAC	3 cut(s)	190, 505, 969
BsnI	GGCC	4 cut(s)	497, 616, 1123, 1147
Bso31I	GGTCTC	1 cut(s)	1876
BsoBI	CYCGRG	2 cut(s)	1215, 1769
Bsp1286I	GDGCHC	1 cut(s)	285
Bsp1407I	TGTACA	1 cut(s)	1710
Bsp143I	GATC	8 cut(s)	154, 1261, 1336, 1501, 1507, 1927, 1966, 2041
BspACI	CCGC	4 cut(s)	10, 1021, 1677, 1985
BspANI	GGCC	4 cut(s)	497, 616, 1123, 1147
BspCNI	CTCAG	5 cut(s)	928, 993, 1470, 1725, 1840
BspLI	GGNNCC	2 cut(s)	570, 1503
BspMI	ACCTGC	1 cut(s)	1944
BspPI	GGATC	7 cut(s)	149, 1496, 1502, 1509, 1922, 1974, 2049
BspTNI	GGTCTC	1 cut(s)	1876
BsrDI	GCAATG	1 cut(s)	1901
BsrFI	RCCGGY	1 cut(s)	802
BsrGI	TGTACA	1 cut(s)	1710
BsrI	ACTGG	2 cut(s)	1393, 1883
BssAI	RCCGGY	1 cut(s)	802
BssECI	CCNNGG	2 cut(s)	450, 617
BssMI	GATC	8 cut(s)	154, 1261, 1336, 1501, 1507, 1927, 1966, 2041
BssNAI	GTATAC	2 cut(s)	129, 1827
BssNI	GRCGYC	1 cut(s)	1071
BssSI	CACGAG	1 cut(s)	1892
BssT1I	CCWWGG	1 cut(s)	450
Bst1107I	GTATAC	2 cut(s)	129, 1827
Bst2BI	CACGAG	1 cut(s)	1892
Bst2UI	CCWGG	2 cut(s)	618, 1831
Bst4CI	ACNGT	3 cut(s)	703, 1245, 1455
Bst6I	CTCTTC	3 cut(s)	476, 860, 2136
BstACI	GRCGYC	1 cut(s)	1071
BstAPI	GCANNNNNTGC	2 cut(s)	635, 2098
BstAUI	TGTACA	1 cut(s)	1710
BstBAI	YACGTR	1 cut(s)	1700
BstC8I	GCNNGC	2 cut(s)	876, 1444
BstDEI	CTNAG	7 cut(s)	383, 936, 980, 1317, 1478, 1733, 1848
BstF5I	GGATG	9 cut(s)	193, 715, 720, 757, 889, 1032, 1054, 1797, 1839
BstHHI	GCGC	1 cut(s)	1953
BstKTI	GATC	8 cut(s)	157, 1264, 1339, 1504, 1510, 1930, 1969, 2044
BstMAI	GTCTC	4 cut(s)	908, 1842, 1844, 1876
BstMBI	GATC	8 cut(s)	154, 1261, 1336, 1501, 1507, 1927, 1966, 2041
BstMWI	GCNNNNNNNGC	4 cut(s)	635, 1226, 1544, 2098
BstNI	CCWGG	2 cut(s)	618, 1831
BstNSI	RCATGY	1 cut(s)	337
BstSCI	CCNGG	2 cut(s)	616, 1829
BstSFI	CTRYAG	2 cut(s)	1302, 2136
BstV1I	GCAGC	5 cut(s)	526, 1238, 1960, 2079, 2085
BstV2I	GAAGAC	1 cut(s)	168
BstX2I	RGATCY	1 cut(s)	1501
BstXI	CCANNNNNNTGG	2 cut(s)	1165, 1878
BstYI	RGATCY	1 cut(s)	1501
BstZ17I	GTATAC	2 cut(s)	129, 1827
Bsu36I	CCTNAGG	1 cut(s)	936
BsuI	GTATCC	1 cut(s)	969
BsuRI	GGCC	4 cut(s)	497, 616, 1123, 1147
BtsCI	GGATG	9 cut(s)	193, 715, 720, 757, 889, 1032, 1054, 1797, 1839
BtsI	GCAGTG	2 cut(s)	1380, 2077
BtsIMutI	CAGTG	4 cut(s)	708, 1380, 1460, 2077
BveI	ACCTGC	1 cut(s)	1944
Cac8I	GCNNGC	2 cut(s)	876, 1444
CfoI	GCGC	1 cut(s)	1953
Cfr10I	RCCGGY	1 cut(s)	802
Cfr13I	GGNCC	3 cut(s)	1018, 1145, 1211
Csp6I	GTAC	5 cut(s)	466, 699, 1711, 1774, 1844
CspAI	ACCGGT	1 cut(s)	802
CviAII	CATG	4 cut(s)	334, 1340, 1535, 1963
CviQI	GTAC	5 cut(s)	466, 699, 1711, 1774, 1844
DdeI	CTNAG	7 cut(s)	383, 936, 980, 1317, 1478, 1733, 1848
DpnI	GATC	8 cut(s)	156, 1263, 1338, 1503, 1509, 1929, 1968, 2043
DpnII	GATC	8 cut(s)	154, 1261, 1336, 1501, 1507, 1927, 1966, 2041
Eam1104I	CTCTTC	3 cut(s)	476, 860, 2136
EarI	CTCTTC	3 cut(s)	476, 860, 2136
Ecl136II	GAGCTC	1 cut(s)	283
Eco130I	CCWWGG	1 cut(s)	450
Eco24I	GRGCYC	1 cut(s)	285
Eco31I	GGTCTC	1 cut(s)	1876
Eco47I	GGWCC	2 cut(s)	1018, 1211
Eco53kI	GAGCTC	1 cut(s)	283
Eco57I	CTGAAG	3 cut(s)	180, 1508, 1805
Eco72I	CACGTG	1 cut(s)	1700
Eco81I	CCTNAGG	1 cut(s)	936
Eco88I	CYCGRG	2 cut(s)	1215, 1769
EcoICRI	GAGCTC	1 cut(s)	283
EcoO109I	RGGNCCY	1 cut(s)	1211
EcoRII	CCWGG	2 cut(s)	616, 1829
EcoT14I	CCWWGG	1 cut(s)	450
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	1 cut(s)	285
ErhI	CCWWGG	1 cut(s)	450
Esp3I	CGTCTC	1 cut(s)	908
FaeI	CATG	4 cut(s)	337, 1343, 1538, 1966
FaqI	GGGAC	3 cut(s)	190, 505, 969
FatI	CATG	4 cut(s)	333, 1339, 1534, 1962
FauNDI	CATATG	1 cut(s)	1076
FbaI	TGATCA	2 cut(s)	1261, 1336
FblI	GTMKAC	3 cut(s)	128, 1362, 1826
Fnu4HI	GCNGC	7 cut(s)	10, 515, 1227, 1677, 1949, 2068, 2099
FokI	GGATG	9 cut(s)	200, 722, 727, 764, 896, 1039, 1041, 1804, 1846
FriOI	GRGCYC	1 cut(s)	285
Fsp4HI	GCNGC	7 cut(s)	10, 515, 1227, 1677, 1949, 2068, 2099
FspBI	CTAG	7 cut(s)	90, 423, 428, 813, 1007, 1283, 1661
GlaI	GCGC	1 cut(s)	1952
GluI	GCNGC	7 cut(s)	10, 515, 1227, 1677, 1949, 2068, 2099
GsuI	CTGGAG	1 cut(s)	1900
HaeIII	GGCC	4 cut(s)	497, 616, 1123, 1147
HapII	CCGG	1 cut(s)	803
HhaI	GCGC	1 cut(s)	1953
Hin1I	GRCGYC	1 cut(s)	1071
Hin1II	CATG	4 cut(s)	337, 1343, 1538, 1966
Hin6I	GCGC	1 cut(s)	1951
HinP1I	GCGC	1 cut(s)	1951
HincII	GTYRAC	1 cut(s)	1581
HindII	GTYRAC	1 cut(s)	1581
HindIII	AAGCTT	2 cut(s)	1444, 1667
HinfI	GANTC	9 cut(s)	34, 139, 276, 470, 581, 1097, 1729, 1735, 1805
HpaII	CCGG	1 cut(s)	803
HphI	GGTGA	8 cut(s)	16, 438, 658, 742, 817, 1733, 1758, 2168
Hpy188I	TCNGA	7 cut(s)	273, 303, 861, 1102, 1488, 1526, 1734
Hpy99I	CGWCG	2 cut(s)	147, 320
HpyCH4III	ACNGT	3 cut(s)	703, 1245, 1455
HpyCH4IV	ACGT	5 cut(s)	917, 1071, 1115, 1699, 1937
HpyCH4V	TGCA	9 cut(s)	4, 608, 629, 665, 1226, 1458, 1559, 1607, 1703
HpyF10VI	GCNNNNNNNGC	4 cut(s)	635, 1226, 1544, 2098
HpyF3I	CTNAG	7 cut(s)	383, 936, 980, 1317, 1478, 1733, 1848
HpySE526I	ACGT	5 cut(s)	917, 1071, 1115, 1699, 1937
Hsp92I	GRCGYC	1 cut(s)	1071
Hsp92II	CATG	4 cut(s)	337, 1343, 1538, 1966
HspAI	GCGC	1 cut(s)	1951
Ksp22I	TGATCA	2 cut(s)	1261, 1336
Kzo9I	GATC	8 cut(s)	154, 1261, 1336, 1501, 1507, 1927, 1966, 2041
LmnI	GCTCC	7 cut(s)	83, 419, 568, 937, 1377, 1695, 1945
Lsp1109I	GCAGC	5 cut(s)	526, 1238, 1960, 2079, 2085
LweI	GCATC	3 cut(s)	13, 1213, 1467
MaeI	CTAG	7 cut(s)	90, 423, 428, 813, 1007, 1283, 1661
MaeII	ACGT	5 cut(s)	917, 1071, 1115, 1699, 1937
MaeIII	GTNAC	9 cut(s)	247, 444, 796, 1067, 1376, 1435, 1764, 1874, 2072
MalI	GATC	8 cut(s)	156, 1263, 1338, 1503, 1509, 1929, 1968, 2043
MboI	GATC	8 cut(s)	154, 1261, 1336, 1501, 1507, 1927, 1966, 2041
MboII	GAAGA	6 cut(s)	173, 493, 847, 874, 877, 2153
MfeI	CAATTG	4 cut(s)	49, 1809, 2093, 2177
MflI	RGATCY	1 cut(s)	1501
MhlI	GDGCHC	1 cut(s)	285
MlyI	GAGTC	3 cut(s)	479, 1744, 1814
MmeI	TCCRAC	4 cut(s)	546, 687, 891, 2019
Mph1103I	ATGCAT	1 cut(s)	6
MseI	TTAA	5 cut(s)	441, 492, 735, 783, 1041
MslI	CAYNNNNRTG	2 cut(s)	1554, 2000
MspA1I	CMGCKG	1 cut(s)	1023
MspI	CCGG	1 cut(s)	803
MspR9I	CCNGG	2 cut(s)	618, 1831
MunI	CAATTG	4 cut(s)	49, 1809, 2093, 2177
MvaI	CCWGG	2 cut(s)	618, 1831
MwoI	GCNNNNNNNGC	4 cut(s)	635, 1226, 1544, 2098
NdeI	CATATG	1 cut(s)	1076
NdeII	GATC	8 cut(s)	154, 1261, 1336, 1501, 1507, 1927, 1966, 2041
NlaIII	CATG	4 cut(s)	337, 1343, 1538, 1966
NlaIV	GGNNCC	2 cut(s)	570, 1503
NmuCI	GTSAC	5 cut(s)	247, 444, 1067, 1764, 2072
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	1 cut(s)	337
PaeR7I	CTCGAG	1 cut(s)	1215
PaqCI	CACCTGC	1 cut(s)	1944
PcsI	WCGNNNNNNNCGW	2 cut(s)	599, 1213
PfeI	GAWTC	6 cut(s)	34, 139, 276, 581, 1097, 1729
PflMI	CCANNNNNTGG	1 cut(s)	1142
PinAI	ACCGGT	1 cut(s)	802
PkrI	GCNGC	7 cut(s)	11, 516, 1228, 1678, 1950, 2069, 2100
PleI	GAGTC	3 cut(s)	478, 1743, 1813
PmaCI	CACGTG	1 cut(s)	1700
PmlI	CACGTG	1 cut(s)	1700
PpsI	GAGTC	3 cut(s)	478, 1743, 1813
Ppu21I	YACGTR	1 cut(s)	1700
PpuMI	RGGWCCY	1 cut(s)	1211
PshBI	ATTAAT	1 cut(s)	735
Psp124BI	GAGCTC	1 cut(s)	285
Psp1406I	AACGTT	1 cut(s)	1937
Psp5II	RGGWCCY	1 cut(s)	1211
Psp6I	CCWGG	2 cut(s)	616, 1829
PspCI	CACGTG	1 cut(s)	1700
PspGI	CCWGG	2 cut(s)	616, 1829
PspN4I	GGNNCC	2 cut(s)	570, 1503
PspPI	GGNCC	3 cut(s)	1018, 1145, 1211
PspPPI	RGGWCCY	1 cut(s)	1211
PspXI	VCTCGAGB	1 cut(s)	1215
PsuI	RGATCY	1 cut(s)	1501
RsaI	GTAC	5 cut(s)	467, 700, 1712, 1775, 1845
RsaNI	GTAC	5 cut(s)	466, 699, 1711, 1774, 1844
RseI	CAYNNNNRTG	2 cut(s)	1554, 2000
SacI	GAGCTC	1 cut(s)	285
SaqAI	TTAA	5 cut(s)	441, 492, 735, 783, 1041
SatI	GCNGC	7 cut(s)	10, 515, 1227, 1677, 1949, 2068, 2099
Sau3AI	GATC	8 cut(s)	154, 1261, 1336, 1501, 1507, 1927, 1966, 2041
Sau96I	GGNCC	3 cut(s)	1018, 1145, 1211
SchI	GAGTC	3 cut(s)	479, 1744, 1814
ScrFI	CCNGG	2 cut(s)	618, 1831
SduI	GDGCHC	1 cut(s)	285
SfaNI	GCATC	3 cut(s)	13, 1213, 1467
SfcI	CTRYAG	2 cut(s)	1302, 2136
Sfr274I	CTCGAG	1 cut(s)	1215
SgrAI	CRCCGGYG	1 cut(s)	802
SinI	GGWCC	2 cut(s)	1018, 1211
SlaI	CTCGAG	1 cut(s)	1215
SmiMI	CAYNNNNRTG	2 cut(s)	1554, 2000
SmlI	CTYRAG	5 cut(s)	365, 1215, 1277, 1542, 2123
SmoI	CTYRAG	5 cut(s)	365, 1215, 1277, 1542, 2123
SpeI	ACTAGT	1 cut(s)	812
SsiI	CCGC	4 cut(s)	10, 1021, 1677, 1985
SspI	AATATT	1 cut(s)	1000
SspMI	CTAG	7 cut(s)	90, 423, 428, 813, 1007, 1283, 1661
SstI	GAGCTC	1 cut(s)	285
StyD4I	CCNGG	2 cut(s)	616, 1829
StyI	CCWWGG	1 cut(s)	450
TaaI	ACNGT	3 cut(s)	703, 1245, 1455
TaiI	ACGT	5 cut(s)	920, 1074, 1118, 1702, 1940
TaqI	TCGA	9 cut(s)	197, 229, 279, 347, 479, 848, 1207, 1216, 1590
TaqII	GACCGA	1 cut(s)	476
TatI	WGTACW	2 cut(s)	698, 1710
TauI	GCSGC	2 cut(s)	12, 1679
TfiI	GAWTC	6 cut(s)	34, 139, 276, 581, 1097, 1729
Tru1I	TTAA	5 cut(s)	441, 492, 735, 783, 1041
Tru9I	TTAA	5 cut(s)	441, 492, 735, 783, 1041
TscAI	CASTG	4 cut(s)	708, 1387, 1460, 2077
TseFI	GTSAC	5 cut(s)	247, 444, 1067, 1764, 2072
TseI	GCWGC	5 cut(s)	514, 1226, 1948, 2067, 2098
Tsp45I	GTSAC	5 cut(s)	247, 444, 1067, 1764, 2072
TspDTI	ATGAA	4 cut(s)	731, 900, 1026, 1580
TspGWI	ACGGA	4 cut(s)	1047, 1364, 1682, 2025
TspRI	CASTG	4 cut(s)	708, 1387, 1460, 2077
Van91I	CCANNNNNTGG	1 cut(s)	1142
VpaK11BI	GGWCC	2 cut(s)	1018, 1211
VspI	ATTAAT	1 cut(s)	735
XapI	RAATTY	2 cut(s)	223, 1127
XbaI	TCTAGA	1 cut(s)	1006
XceI	RCATGY	1 cut(s)	337
XcmI	CCANNNNNNNNNTGG	1 cut(s)	903
XhoI	CTCGAG	1 cut(s)	1215
XmiI	GTMKAC	3 cut(s)	128, 1362, 1826
XspI	CTAG	7 cut(s)	90, 423, 428, 813, 1007, 1283, 1661
ZraI	GACGTC	1 cut(s)	1072
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.