RchiOBHm_Chr7g0216301

Belongs to the DHHC palmitoyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014280
Physical Location & Seq
Forward (+)
33895870 .. 33900944
5075 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19353

Sequence Viewer

Length: 1899 bp
ATGGCTCGCCGTCATGGATGGGAACTCCCTGCTCACACTTTTCAGGTCGTGGCTATAACCGTTTTTTTCTTGCTAACGGTTGCATATTATGCCTTCTTTGCTCCCTTTCTTGGAAATGACATCTATGAGTATGTGGCAATTGGTGTATATTCTGCGTTGGTGTTTTCTGTATTCATACTTTATGTTCGGTGTACAGCAATTGATCCTGCTGATTCTGGAATACTACTTGAAGCTGACAAGACATTTTCCCACAAAGAAAACAATAACACAGGGAACATTTCATTCAGTTATGGAGGACAAGTGGACAGCCATGGTGCAAGTTGGTGCTCAAAAGTTGGAGGTTTCTTCTGTGGTTGTCTTGTAAGAGAAGATTGCCGCAAACGTGAAGATCTCTTGCAATTACAGCAGCAATCTGGAGAGGATCCTCTCTTCTGCACTTTGTGCAATGCTGAGGTCGGCAAGTTCAGCAAACATTGTAGAAGCTGTGATAAATGTGTTGATGGATTTGATCATCATTGTCGGTGGCTAAATAATTGTGTGGGGAGGAAAAATTATATCACATTTGTGTCTCTTATGGCTGCAAGCCTTGTTTGGCTTATTGTTGAATGTGGTGTTGGTATTGCTGTCCTTGTTCGATGCTTTGCTGATAAAAATAGTATGAAAGGGCAGATAGCTGATAGGCTTGGAGTTGGTTTCTCTCAGCCCCCCTTTGCCACTGTGGTGGCCCTATGTACAGCTATTTCCTTCCTTGCCATTTATCCTCTGGCAGAGCTGTTCTTTTTCCACATAATTTTGATTCGAAAGGGTATTACAACCTATGAGTATGTCGTTGCGATGAGAACACAAAGTGAACCTCCTGGACCATCAGTGGATGGAGGCGATCAGCAAAGTATTGCATCTTCTCCAACAAGTTCAGCTGTGACTGCCATGAGTGGAAGAAGCTCTCTTGGAATGAATTTGCCATACAAAGGTGCTTGGTGCACCCCTCCAAGGATCTTTGTGGATCACCAGATGCAGGATGAAATTATTCCACATTTGGAGCCAGGACGCTTACCATCGACACTGGATCCAGATGCAATACAGCAGCCTGATAAGGCAAAAAAATTACCCCAGCGTCCAGTCCGAATAAGTGCATGGAAGCTTGCTAAACTAGATGCTAATGAGGCAACCAAAGCAGCTGCCAAAGCTAGAGCATCATCATCTGTGCTTCGTCCAATTAGTTCTGAACATCATCCGTATGAAGCTGAATATGTATCGAGCAGTAATGTGAGTGGAAGAAGTAGTCCAACTAGTACTGATCAGGGGTTTAACAACAGAAACACTAGAACTGGGACATCAAAATTATCTCCTATGAGCTCATACCCACCTAGTCGTGCTAGCAGAGAAGATGTTGAAATTGGACGTTACGCTGGTAGTAACTTAAGCAGTCCTCATGTCTCTAGTCTCACCCCTTCTCCTCTAGAGCAGAAAACTTCAAACTTGGATCAGTTCAACCCCATTTATCAGTCATCAAATGATCAATCTCCATTGTCAGGAAAGCAAAGTGAAGCAAAGGTAAATGTAGGACAGATGCCTACAGAAAAAGGTAACTCAATTCTTCAAGGAAATGGGAGATCGTCAGTTTTTTGGGATCAAGAAGCGGGACGTTTTGTCTCATCTTCCAGATCTGGTGGTTCCTCCTCAGGAGCAGAGCTATTGTACACGGGACAATCCATATTTTTTGGTGGACCTGTTCTCAATGAACCACCCGCTAGAGGGACAAGGAATATTGGTTCACTGGCTTCCGAGCATGACAGAGGTTCTACATATCAACAGGGTAGATCACAGAGAGGTGGCCAGCTTCCCGTGTTTGTTCCAAGTGAGTCTGAGAAAAATCAGTTCTCTTCTAGATTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DHHC

Protein Analysis

632

Amino Acids

68.88

Weight (kDa)

8.24

Isoelectric Point (pI)

55.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHC PF01529 142 - 276 5.3e-32 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33640
fragaria_vesca FvH4_5g25830 FvH4_5g25830 FvH4_5g25830 FvH4_5g25830
prunus_persica Prupe.5G007200_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0216301
rosa_laevigata RLG00000002583
rosa_multiflora Rmu_co8515551.1_g000001 Rmu_sc0004663.1_g000002
rosa_roxburghii Rroxscaffold_3G00243350
rosa_rugosa Rorug07G0159800
rosa_samantha Rh7AG297100 Rh7BG289300 Rh7CG317400 Rh7DG299600
rosa_wichuraiana Rw7G025270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 376, 1640, 1749
AclWI GGATC 9 cut(s) 197, 416, 429, 1001, 1011, 1061, 1074, 1491, 1638
AcoI YGGCCR 1 cut(s) 1834
AcsI RAATTY 1 cut(s) 955
AdeI CACNNNGTG 2 cut(s) 441, 848
AfaI GTAC 4 cut(s) 193, 733, 1294, 1700
AfiI CCNNNNNNNGG 7 cut(s) 110, 968, 990, 1015, 1532, 1754, 1755
AflII CTTAAG 1 cut(s) 1420
AgsI TTSAA 6 cut(s) 230, 605, 1394, 1476, 1492, 1601
AhdI GACNNNNNGTC 1 cut(s) 1649
AhlI ACTAGT 1 cut(s) 1289
AjnI CCWGG 2 cut(s) 856, 1042
AjuI GAANNNNNNNTTGG 2 cut(s) 597, 629
AleI CACNNNNGTG 2 cut(s) 563, 719
AloI GAACNNNNNNTCC 2 cut(s) 1756, 1788
Alw21I GWGCWC 3 cut(s) 329, 983, 1358
Alw26I GTCTC 4 cut(s) 573, 1441, 1448, 1657
Alw44I GTGCAC 1 cut(s) 979
AlwI GGATC 9 cut(s) 197, 416, 429, 1001, 1011, 1061, 1074, 1491, 1638
AoxI GGCC 2 cut(s) 723, 1834
ApaLI GTGCAC 1 cut(s) 979
ApeKI GCWGC 5 cut(s) 406, 578, 1084, 1175, 1178
ApoI RAATTY 1 cut(s) 955
Asp700I GAANNNNTTC 1 cut(s) 1026
AspS9I GGNCC 3 cut(s) 724, 860, 1727
AsuHPI GGTGA 2 cut(s) 998, 1438
AsuII TTCGAA 1 cut(s) 799
AsuNHI GCTAGC 1 cut(s) 1376
AvaII GGWCC 2 cut(s) 860, 1727
AxyI CCTNAGG 1 cut(s) 1681
BaeGI GKGCMC 1 cut(s) 983
BalI TGGCCA 1 cut(s) 1836
BamHI GGATCC 2 cut(s) 421, 1066
BanII GRGCYC 1 cut(s) 1358
BarI GAAGNNNNNNTAC 2 cut(s) 883, 915
Bbv12I GWGCWC 3 cut(s) 329, 983, 1358
BbvCI CCTCAGC 1 cut(s) 450
BbvI GCAGC 5 cut(s) 418, 565, 1096, 1165, 1187
BccI CCATC 5 cut(s) 12, 494, 866, 871, 1063
BciT130I CCWGG 2 cut(s) 858, 1044
BclI TGATCA 3 cut(s) 508, 1297, 1516
BcoDI GTCTC 4 cut(s) 573, 1441, 1448, 1657
BcuI ACTAGT 1 cut(s) 1289
BfmI CTRYAG 1 cut(s) 1575
BfrI CTTAAG 1 cut(s) 1420
BglII AGATCT 2 cut(s) 388, 1664
BisI GCNGC 6 cut(s) 376, 407, 579, 1085, 1176, 1179
BlsI GCNGC 6 cut(s) 377, 408, 580, 1086, 1177, 1180
BmcAI AGTACT 1 cut(s) 1294
Bme1390I CCNGG 2 cut(s) 858, 1044
Bme18I GGWCC 2 cut(s) 860, 1727
BmeRI GACNNNNNGTC 1 cut(s) 1649
BmgT120I GGNCC 3 cut(s) 724, 860, 1727
BmiI GGNNCC 4 cut(s) 423, 1041, 1068, 1675
BmrFI CCNGG 2 cut(s) 858, 1044
BmrI ACTGGG 1 cut(s) 1338
BmsI GCATC 7 cut(s) 626, 905, 1002, 1063, 1144, 1202, 1560
BmtI GCTAGC 1 cut(s) 1380
BmuI ACTGGG 1 cut(s) 1338
BpmI CTGGAG 1 cut(s) 435
Bpu10I CCTNAGC 1 cut(s) 450
Bpu14I TTCGAA 1 cut(s) 799
BsaJI CCNNGG 2 cut(s) 310, 989
BsaXI ACNNNNNCTCC 4 cut(s) 285, 315, 1438, 1468
Bsc4I CCNNNNNNNGG 7 cut(s) 110, 968, 990, 1015, 1532, 1754, 1755
Bse1I ACTGG 4 cut(s) 1068, 1118, 1333, 1782
Bse21I CCTNAGG 1 cut(s) 1681
Bse3DI GCAATG 1 cut(s) 451
BseBI CCWGG 2 cut(s) 858, 1044
BseDI CCNNGG 2 cut(s) 310, 989
BseGI GGATG 4 cut(s) 23, 877, 1024, 1231
BseLI CCNNNNNNNGG 7 cut(s) 110, 968, 990, 1015, 1532, 1754, 1755
BseMI GCAATG 1 cut(s) 451
BseMII CTCAG 4 cut(s) 441, 713, 1695, 1857
BseNI ACTGG 4 cut(s) 1068, 1118, 1333, 1782
BseRI GAGGAG 2 cut(s) 1446, 1669
BseSI GKGCMC 1 cut(s) 983
BseXI GCAGC 5 cut(s) 418, 565, 1096, 1165, 1187
BseYI CCCAGC 1 cut(s) 1110
BsgI GTGCAG 1 cut(s) 418
BshFI GGCC 2 cut(s) 725, 1836
BsiHKAI GWGCWC 3 cut(s) 329, 983, 1358
BslFI GGGAC 4 cut(s) 1345, 1656, 1719, 1771
BslI CCNNNNNNNGG 7 cut(s) 110, 968, 990, 1015, 1532, 1754, 1755
BsmAI GTCTC 4 cut(s) 573, 1441, 1448, 1657
BsmFI GGGAC 4 cut(s) 1345, 1656, 1719, 1771
BsnI GGCC 2 cut(s) 725, 1836
Bsp119I TTCGAA 1 cut(s) 799
Bsp1286I GDGCHC 3 cut(s) 329, 983, 1358
Bsp1407I TGTACA 3 cut(s) 191, 731, 1698
Bsp19I CCATGG 1 cut(s) 310
BspACI CCGC 3 cut(s) 376, 1640, 1749
BspANI GGCC 2 cut(s) 725, 1836
BspCNI CTCAG 4 cut(s) 442, 712, 1694, 1858
BspLI GGNNCC 4 cut(s) 423, 1041, 1068, 1675
BspOI GCTAGC 1 cut(s) 1380
BspPI GGATC 9 cut(s) 197, 416, 429, 1001, 1011, 1061, 1074, 1491, 1638
BspT104I TTCGAA 1 cut(s) 799
BspTI CTTAAG 1 cut(s) 1420
BsrDI GCAATG 1 cut(s) 451
BsrGI TGTACA 3 cut(s) 191, 731, 1698
BsrI ACTGG 4 cut(s) 1068, 1118, 1333, 1782
BssECI CCNNGG 2 cut(s) 310, 989
BssT1I CCWWGG 2 cut(s) 310, 989
Bst2UI CCWGG 2 cut(s) 858, 1044
Bst4CI ACNGT 3 cut(s) 61, 79, 718
Bst6I CTCTTC 2 cut(s) 434, 1888
BstAFI CTTAAG 1 cut(s) 1420
BstAPI GCANNNNNTGC 2 cut(s) 89, 441
BstAUI TGTACA 3 cut(s) 191, 731, 1698
BstBI TTCGAA 1 cut(s) 799
BstC8I GCNNGC 5 cut(s) 7, 583, 1143, 1378, 1838
BstDEI CTNAG 4 cut(s) 450, 699, 1681, 1866
BstDSI CCRYGG 1 cut(s) 310
BstF5I GGATG 4 cut(s) 23, 877, 1024, 1231
BstMAI GTCTC 4 cut(s) 573, 1441, 1448, 1657
BstMWI GCNNNNNNNGC 9 cut(s) 89, 98, 403, 441, 465, 923, 1163, 1172, 1184
BstNI CCWGG 2 cut(s) 858, 1044
BstSCI CCNGG 2 cut(s) 856, 1042
BstSFI CTRYAG 1 cut(s) 1575
BstSLI GKGCMC 1 cut(s) 983
BstV1I GCAGC 5 cut(s) 418, 565, 1096, 1165, 1187
BstX2I RGATCY 5 cut(s) 388, 421, 993, 1066, 1664
BstXI CCANNNNNNTGG 1 cut(s) 721
BstYI RGATCY 5 cut(s) 388, 421, 993, 1066, 1664
Bsu36I CCTNAGG 1 cut(s) 1681
BsuRI GGCC 2 cut(s) 725, 1836
BtgI CCRYGG 1 cut(s) 310
BtgZI GCGATG 1 cut(s) 848
BtsCI GGATG 4 cut(s) 23, 877, 1024, 1231
BtsIMutI CAGTG 4 cut(s) 714, 873, 1061, 1775
Cac8I GCNNGC 5 cut(s) 7, 583, 1143, 1378, 1838
Cfr13I GGNCC 3 cut(s) 724, 860, 1727
CseI GACGC 2 cut(s) 1056, 1103
Csp6I GTAC 4 cut(s) 192, 732, 1293, 1699
CviAII CATG 6 cut(s) 14, 311, 928, 1134, 1433, 1790
CviQI GTAC 4 cut(s) 192, 732, 1293, 1699
DdeI CTNAG 4 cut(s) 450, 699, 1681, 1866
DraIII CACNNNGTG 2 cut(s) 441, 848
DriI GACNNNNNGTC 1 cut(s) 1649
EaeI YGGCCR 1 cut(s) 1834
Eam1104I CTCTTC 2 cut(s) 434, 1888
Eam1105I GACNNNNNGTC 1 cut(s) 1649
EarI CTCTTC 2 cut(s) 434, 1888
Ecl136II GAGCTC 1 cut(s) 1356
Eco130I CCWWGG 2 cut(s) 310, 989
Eco24I GRGCYC 1 cut(s) 1358
Eco47I GGWCC 2 cut(s) 860, 1727
Eco53kI GAGCTC 1 cut(s) 1356
Eco81I CCTNAGG 1 cut(s) 1681
EcoICRI GAGCTC 1 cut(s) 1356
EcoRII CCWGG 2 cut(s) 856, 1042
EcoT14I CCWWGG 2 cut(s) 310, 989
EcoT38I GRGCYC 1 cut(s) 1358
ErhI CCWWGG 2 cut(s) 310, 989
FaeI CATG 6 cut(s) 17, 314, 931, 1137, 1436, 1793
FaqI GGGAC 4 cut(s) 1345, 1656, 1719, 1771
FatI CATG 6 cut(s) 13, 310, 927, 1133, 1432, 1789
FauI CCCGC 2 cut(s) 1633, 1756
FbaI TGATCA 3 cut(s) 508, 1297, 1516
Fnu4HI GCNGC 6 cut(s) 376, 407, 579, 1085, 1176, 1179
FokI GGATG 4 cut(s) 30, 884, 1031, 1218
FriOI GRGCYC 1 cut(s) 1358
Fsp4HI GCNGC 6 cut(s) 376, 407, 579, 1085, 1176, 1179
GluI GCNGC 6 cut(s) 376, 407, 579, 1085, 1176, 1179
GsaI CCCAGC 1 cut(s) 1114
GsuI CTGGAG 1 cut(s) 435
HaeIII GGCC 2 cut(s) 725, 1836
HgaI GACGC 2 cut(s) 1056, 1103
Hin1II CATG 6 cut(s) 17, 314, 931, 1137, 1436, 1793
HindIII AAGCTT 1 cut(s) 1139
HinfI GANTC 3 cut(s) 212, 796, 1862
HphI GGTGA 2 cut(s) 998, 1438
Hpy166II GTNNAC 7 cut(s) 192, 304, 851, 981, 1701, 1727, 1775
Hpy188I TCNGA 4 cut(s) 1124, 1225, 1786, 1867
Hpy188III TCNNGA 9 cut(s) 216, 414, 1070, 1460, 1533, 1634, 1662, 1683, 1887
Hpy8I GTNNAC 7 cut(s) 192, 304, 851, 981, 1701, 1727, 1775
HpyAV CCTTC 3 cut(s) 103, 754, 1461
HpyCH4III ACNGT 3 cut(s) 61, 79, 718
HpyCH4IV ACGT 3 cut(s) 382, 1402, 1645
HpyF10VI GCNNNNNNNGC 9 cut(s) 89, 98, 403, 441, 465, 923, 1163, 1172, 1184
HpyF3I CTNAG 4 cut(s) 450, 699, 1681, 1866
HpySE526I ACGT 3 cut(s) 382, 1402, 1645
Hsp92II CATG 6 cut(s) 17, 314, 931, 1137, 1436, 1793
Ksp22I TGATCA 3 cut(s) 508, 1297, 1516
LmnI GCTCC 3 cut(s) 106, 1039, 1685
Lsp1109I GCAGC 5 cut(s) 418, 565, 1096, 1165, 1187
LweI GCATC 7 cut(s) 626, 905, 1002, 1063, 1144, 1202, 1560
MaeII ACGT 3 cut(s) 382, 1402, 1645
MaeIII GTNAC 4 cut(s) 919, 1403, 1415, 1586
MfeI CAATTG 2 cut(s) 138, 198
MflI RGATCY 5 cut(s) 388, 421, 993, 1066, 1664
MhlI GDGCHC 3 cut(s) 329, 983, 1358
MlsI TGGCCA 1 cut(s) 1836
MluNI TGGCCA 1 cut(s) 1836
MlyI GAGTC 1 cut(s) 1871
MmeI TCCRAC 3 cut(s) 316, 929, 1310
Mox20I TGGCCA 1 cut(s) 1836
MroXI GAANNNNTTC 1 cut(s) 1026
MscI TGGCCA 1 cut(s) 1836
MseI TTAA 2 cut(s) 1308, 1421
MslI CAYNNNNRTG 3 cut(s) 563, 719, 1236
Msp20I TGGCCA 1 cut(s) 1836
MspA1I CMGCKG 2 cut(s) 917, 1178
MspCI CTTAAG 1 cut(s) 1420
MspR9I CCNGG 2 cut(s) 858, 1044
MunI CAATTG 2 cut(s) 138, 198
MvaI CCWGG 2 cut(s) 858, 1044
MwoI GCNNNNNNNGC 9 cut(s) 89, 98, 403, 441, 465, 923, 1163, 1172, 1184
NcoI CCATGG 1 cut(s) 310
NheI GCTAGC 1 cut(s) 1376
NlaIII CATG 6 cut(s) 17, 314, 931, 1137, 1436, 1793
NlaIV GGNNCC 4 cut(s) 423, 1041, 1068, 1675
NmuCI GTSAC 1 cut(s) 919
NspV TTCGAA 1 cut(s) 799
OliI CACNNNNGTG 2 cut(s) 563, 719
PdmI GAANNNNTTC 1 cut(s) 1026
PfeI GAWTC 2 cut(s) 212, 796
PfoI TCCNGGA 1 cut(s) 856
PkrI GCNGC 6 cut(s) 377, 408, 580, 1086, 1177, 1180
PleI GAGTC 1 cut(s) 1870
PpsI GAGTC 1 cut(s) 1870
Psp124BI GAGCTC 1 cut(s) 1358
Psp6I CCWGG 2 cut(s) 856, 1042
PspFI CCCAGC 1 cut(s) 1110
PspGI CCWGG 2 cut(s) 856, 1042
PspN4I GGNNCC 4 cut(s) 423, 1041, 1068, 1675
PspPI GGNCC 3 cut(s) 724, 860, 1727
PsuI RGATCY 5 cut(s) 388, 421, 993, 1066, 1664
PvuII CAGCTG 2 cut(s) 917, 1178
RsaI GTAC 4 cut(s) 193, 733, 1294, 1700
RsaNI GTAC 4 cut(s) 192, 732, 1293, 1699
RseI CAYNNNNRTG 3 cut(s) 563, 719, 1236
SacI GAGCTC 1 cut(s) 1358
SaqAI TTAA 2 cut(s) 1308, 1421
SatI GCNGC 6 cut(s) 376, 407, 579, 1085, 1176, 1179
Sau96I GGNCC 3 cut(s) 724, 860, 1727
ScaI AGTACT 1 cut(s) 1294
SchI GAGTC 1 cut(s) 1871
ScrFI CCNGG 2 cut(s) 858, 1044
SduI GDGCHC 3 cut(s) 329, 983, 1358
SfaNI GCATC 7 cut(s) 626, 905, 1002, 1063, 1144, 1202, 1560
SfcI CTRYAG 1 cut(s) 1575
SfuI TTCGAA 1 cut(s) 799
SinI GGWCC 2 cut(s) 860, 1727
SmiMI CAYNNNNRTG 3 cut(s) 563, 719, 1236
SmlI CTYRAG 1 cut(s) 1420
SmoI CTYRAG 1 cut(s) 1420
SpeI ACTAGT 1 cut(s) 1289
SsiI CCGC 3 cut(s) 376, 1640, 1749
SspI AATATT 1 cut(s) 1768
SstI GAGCTC 1 cut(s) 1358
StyD4I CCNGG 2 cut(s) 856, 1042
StyI CCWWGG 2 cut(s) 310, 989
TaaI ACNGT 3 cut(s) 61, 79, 718
TaiI ACGT 3 cut(s) 385, 1405, 1648
TaqI TCGA 4 cut(s) 634, 799, 1058, 1256
TatI WGTACW 4 cut(s) 191, 731, 1292, 1698
TauI GCSGC 1 cut(s) 378
TfiI GAWTC 2 cut(s) 212, 796
Tru1I TTAA 2 cut(s) 1308, 1421
Tru9I TTAA 2 cut(s) 1308, 1421
TscAI CASTG 4 cut(s) 721, 873, 1068, 1782
TseFI GTSAC 1 cut(s) 919
TseI GCWGC 5 cut(s) 406, 578, 1084, 1175, 1178
Tsp45I GTSAC 1 cut(s) 919
TspDTI ATGAA 7 cut(s) 163, 270, 674, 968, 1035, 1254, 1755
TspGWI ACGGA 1 cut(s) 1224
TspRI CASTG 4 cut(s) 721, 873, 1068, 1782
Vha464I CTTAAG 1 cut(s) 1420
VneI GTGCAC 1 cut(s) 979
VpaK11BI GGWCC 2 cut(s) 860, 1727
XapI RAATTY 1 cut(s) 955
XbaI TCTAGA 2 cut(s) 1459, 1886
XcmI CCANNNNNNNNNTGG 1 cut(s) 760
XmnI GAANNNNTTC 1 cut(s) 1026
ZrmI AGTACT 1 cut(s) 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.