RLG00000000427

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017911
Physical Location & Seq
Reverse (-)
936579 .. 937735
1157 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000427

Sequence Viewer

Length: 405 bp
ATGTACGGTGGATCAGCTCTTCCGGGCCCCTCCGACCCATCAAGCTCGTCCTCGTGGAGGGAGGATTCGTTTGGGATAGAGGTGCTATTAGAGCCCTTTTCCGAAACGGAATCCGAAAGTCAGGAGGGCAATTCCCTCCATTCTGCCGTCCCAAGGGCCGCCCGCGATGAGGCGGGCCCCTCCCATGAGTGGGTGCTTGCTAACGAGCGGTTTTCCGCTCTACTGGCACGGCACCTCCAAAAACACGCAGAAAAGTCGGCGGTGATAGCTAGGTATCCGCAGCTCCGTGAGGCGGATTTCCATCATTTGGGCCAAAAAATGGCCCAAAGCTTTGATATTGATGTGAAATCTCCCTCCGAGATTGAAGCCCTTGCGGCCTCGGAAGAGTTACGGGACCTACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.74

Weight (kDa)

4.86

Isoelectric Point (pI)

72.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017911)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00660
rosa_chinensis RchiOBHm_Chr7g0235961 RchiOBHm_MTg0499091
rosa_laevigata RLG00000000275 RLG00000000277 RLG00000000406 RLG00000000427 RLG00000000481
rosa_roxburghii Rroxscaffold_2G00119470
rosa_samantha Rh3BG350300 Rh6BG169000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 231
AccB7I CCANNNNNTGG 2 cut(s) 307, 319
AccBSI CCGCTC 2 cut(s) 208, 218
AccII CGCG 1 cut(s) 165
AciI CCGC 9 cut(s) 159, 163, 173, 208, 216, 260, 278, 293, 374
AclWI GGATC 1 cut(s) 19
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 8 cut(s) 57, 153, 169, 189, 190, 292, 307, 319
AgsI TTSAA 1 cut(s) 365
AluBI AGCT 5 cut(s) 17, 45, 269, 283, 330
AluI AGCT 5 cut(s) 17, 45, 269, 283, 330
AlwI GGATC 1 cut(s) 19
AoxI GGCC 6 cut(s) 25, 156, 175, 310, 321, 375
ApaI GGGCCC 2 cut(s) 29, 179
ApeKI GCWGC 1 cut(s) 280
AspS9I GGNCC 8 cut(s) 25, 26, 156, 175, 176, 310, 322, 394
AsuC2I CCSGG 1 cut(s) 24
AsuHPI GGTGA 1 cut(s) 274
AvaII GGWCC 1 cut(s) 394
BaeGI GKGCMC 2 cut(s) 29, 179
BanI GGYRCC 1 cut(s) 231
BanII GRGCYC 3 cut(s) 29, 96, 179
BauI CACGAG 1 cut(s) 52
BbvI GCAGC 1 cut(s) 292
BccI CCATC 2 cut(s) 46, 309
BceAI ACGGC 2 cut(s) 131, 245
BcgI CGANNNNNNTGC 2 cut(s) 237, 271
BciVI GTATCC 1 cut(s) 285
BcnI CCSGG 1 cut(s) 24
BfaI CTAG 1 cut(s) 270
BfuI GTATCC 1 cut(s) 285
BglI GCCNNNNNGGC 1 cut(s) 374
BisI GCNGC 3 cut(s) 159, 281, 375
BlsI GCNGC 3 cut(s) 160, 282, 376
Bme1390I CCNGG 1 cut(s) 24
Bme18I GGWCC 1 cut(s) 394
BmgT120I GGNCC 8 cut(s) 25, 26, 156, 175, 176, 310, 322, 394
BmiI GGNNCC 6 cut(s) 27, 28, 177, 178, 233, 395
BmrFI CCNGG 1 cut(s) 24
BpuMI CCSGG 1 cut(s) 24
BsaBI GATNNNNATC 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 152, 378
BsaXI ACNNNNNCTCC 4 cut(s) 53, 83, 219, 249
Bsc4I CCNNNNNNNGG 8 cut(s) 57, 153, 169, 189, 190, 292, 307, 319
Bse1I ACTGG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 300
BseDI CCNNGG 2 cut(s) 152, 378
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 8 cut(s) 57, 153, 169, 189, 190, 292, 307, 319
BseNI ACTGG 1 cut(s) 228
BseSI GKGCMC 2 cut(s) 29, 179
BseXI GCAGC 1 cut(s) 292
Bsh1236I CGCG 1 cut(s) 165
BshFI GGCC 6 cut(s) 27, 158, 177, 312, 323, 377
BshNI GGYRCC 1 cut(s) 231
BsiSI CCGG 1 cut(s) 23
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 8 cut(s) 57, 153, 169, 189, 190, 292, 307, 319
BsmFI GGGAC 1 cut(s) 134
BsnI GGCC 6 cut(s) 27, 158, 177, 312, 323, 377
Bsp120I GGGCCC 2 cut(s) 25, 175
Bsp1286I GDGCHC 3 cut(s) 29, 96, 179
Bsp143I GATC 1 cut(s) 11
BspACI CCGC 9 cut(s) 159, 163, 173, 208, 216, 260, 278, 293, 374
BspANI GGCC 6 cut(s) 27, 158, 177, 312, 323, 377
BspFNI CGCG 1 cut(s) 165
BspLI GGNNCC 6 cut(s) 27, 28, 177, 178, 233, 395
BspPI GGATC 1 cut(s) 19
BspQI GCTCTTC 1 cut(s) 24
BspT107I GGYRCC 1 cut(s) 231
BsrBI CCGCTC 2 cut(s) 208, 218
BsrI ACTGG 1 cut(s) 228
BssECI CCNNGG 2 cut(s) 152, 378
BssMI GATC 1 cut(s) 11
BssSI CACGAG 1 cut(s) 52
BssT1I CCWWGG 1 cut(s) 152
Bst2BI CACGAG 1 cut(s) 52
Bst4CI ACNGT 1 cut(s) 8
Bst6I CTCTTC 2 cut(s) 24, 378
BstC8I GCNNGC 3 cut(s) 163, 175, 198
BstENI CCTNNNNNAGG 1 cut(s) 55
BstFNI CGCG 1 cut(s) 165
BstKTI GATC 1 cut(s) 14
BstMBI GATC 1 cut(s) 11
BstMWI GCNNNNNNNGC 4 cut(s) 91, 224, 266, 374
BstSCI CCNGG 1 cut(s) 22
BstSLI GKGCMC 2 cut(s) 29, 179
BstUI CGCG 1 cut(s) 165
BstV1I GCAGC 1 cut(s) 292
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 6 cut(s) 27, 158, 177, 312, 323, 377
BtgZI GCGATG 1 cut(s) 180
Cac8I GCNNGC 3 cut(s) 163, 175, 198
Cfr13I GGNCC 8 cut(s) 25, 26, 156, 175, 176, 310, 322, 394
Csp6I GTAC 1 cut(s) 4
CviAII CATG 1 cut(s) 185
CviQI GTAC 1 cut(s) 4
DpnI GATC 1 cut(s) 13
DpnII GATC 1 cut(s) 11
Eam1104I CTCTTC 2 cut(s) 24, 378
EarI CTCTTC 2 cut(s) 24, 378
EciI GGCGGA 1 cut(s) 308
Eco130I CCWWGG 1 cut(s) 152
Eco24I GRGCYC 3 cut(s) 29, 96, 179
Eco47I GGWCC 1 cut(s) 394
EcoNI CCTNNNNNAGG 1 cut(s) 55
EcoO109I RGGNCCY 3 cut(s) 26, 176, 394
EcoT14I CCWWGG 1 cut(s) 152
EcoT38I GRGCYC 3 cut(s) 29, 96, 179
ErhI CCWWGG 1 cut(s) 152
FaeI CATG 1 cut(s) 188
FaiI YATR 1 cut(s) 186
FaqI GGGAC 1 cut(s) 134
FatI CATG 1 cut(s) 184
FauI CCCGC 2 cut(s) 166, 170
Fnu4HI GCNGC 3 cut(s) 159, 281, 375
FriOI GRGCYC 3 cut(s) 29, 96, 179
Fsp4HI GCNGC 3 cut(s) 159, 281, 375
FspBI CTAG 1 cut(s) 270
GluI GCNGC 3 cut(s) 159, 281, 375
HaeIII GGCC 6 cut(s) 27, 158, 177, 312, 323, 377
HapII CCGG 1 cut(s) 23
Hin1II CATG 1 cut(s) 188
HindIII AAGCTT 1 cut(s) 328
HinfI GANTC 2 cut(s) 65, 110
HpaII CCGG 1 cut(s) 23
HphI GGTGA 1 cut(s) 274
Hpy188I TCNGA 5 cut(s) 34, 103, 115, 358, 382
Hpy188III TCNNGA 1 cut(s) 122
HpyCH4III ACNGT 1 cut(s) 8
HpyF10VI GCNNNNNNNGC 4 cut(s) 91, 224, 266, 374
Hsp92II CATG 1 cut(s) 188
Kzo9I GATC 1 cut(s) 11
LguI GCTCTTC 1 cut(s) 24
LmnI GCTCC 1 cut(s) 288
LpnPI CCDG 3 cut(s) 36, 107, 209
Lsp1109I GCAGC 1 cut(s) 292
MaeI CTAG 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 387
MalI GATC 1 cut(s) 13
MbiI CCGCTC 2 cut(s) 208, 218
MboI GATC 1 cut(s) 11
MboII GAAGA 2 cut(s) 11, 395
MhlI GDGCHC 3 cut(s) 29, 96, 179
MluCI AATT 1 cut(s) 130
MmeI TCCRAC 1 cut(s) 57
MspI CCGG 1 cut(s) 23
MspR9I CCNGG 1 cut(s) 24
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 4 cut(s) 91, 224, 266, 374
NciI CCSGG 1 cut(s) 24
NdeII GATC 1 cut(s) 11
NlaIII CATG 1 cut(s) 188
NlaIV GGNNCC 6 cut(s) 27, 28, 177, 178, 233, 395
PciSI GCTCTTC 1 cut(s) 24
PfeI GAWTC 2 cut(s) 65, 110
PflMI CCANNNNNTGG 2 cut(s) 307, 319
PkrI GCNGC 3 cut(s) 160, 282, 376
PpuMI RGGWCCY 1 cut(s) 394
Psp5II RGGWCCY 1 cut(s) 394
PspN4I GGNNCC 6 cut(s) 27, 28, 177, 178, 233, 395
PspOMI GGGCCC 2 cut(s) 25, 175
PspPI GGNCC 8 cut(s) 25, 26, 156, 175, 176, 310, 322, 394
PspPPI RGGWCCY 1 cut(s) 394
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 24
SatI GCNGC 3 cut(s) 159, 281, 375
Sau3AI GATC 1 cut(s) 11
Sau96I GGNCC 8 cut(s) 25, 26, 156, 175, 176, 310, 322, 394
ScrFI CCNGG 1 cut(s) 24
SduI GDGCHC 3 cut(s) 29, 96, 179
SetI ASST 9 cut(s) 19, 47, 84, 237, 271, 275, 285, 332, 399
SinI GGWCC 1 cut(s) 394
Sse9I AATT 1 cut(s) 130
SsiI CCGC 9 cut(s) 159, 163, 173, 208, 216, 260, 278, 293, 374
SspMI CTAG 1 cut(s) 270
StyD4I CCNGG 1 cut(s) 22
StyI CCWWGG 1 cut(s) 152
TaaI ACNGT 1 cut(s) 8
TasI AATT 1 cut(s) 130
TauI GCSGC 2 cut(s) 161, 377
TfiI GAWTC 2 cut(s) 65, 110
TseI GCWGC 1 cut(s) 280
TspGWI ACGGA 2 cut(s) 122, 275
Van91I CCANNNNNTGG 2 cut(s) 307, 319
VpaK11BI GGWCC 1 cut(s) 394
XagI CCTNNNNNAGG 1 cut(s) 55
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.