RLG00000003582
WRKY Family

Plant zinc cluster domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0016930
Physical Location & Seq
Reverse (-)
50282919 .. 50292090
9172 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003582

Sequence Viewer

Length: 870 bp
ATGAATTCAGGGTTGGATTTCAAACTCCATGAGGTTGCTAAAACTAGTCTTCAACACGCGCACCACCTAGTTGCTTGTATGTCTGACAATAACCAAGAGAGAAGTGTTCAAGAAGTGAGCCTTGTTGCTCAAGATGCAGTGAAGGAATTCAGAAAACTACTTTCCCTACTTGATGGATCAATGTCATCAAATCACAAGAGGATCAGAAAGGGTCCTTTGCCGAAATCCAGTGACATAAACCAGGATGAAATGATGGACCTTAGTCCTAATTGTTCTTCATCTCATATCAAAACGCACGACCGTGCTCATCAGCCTTGCTTGGTTACACATTTTTTTAGCTCCCTTCAGAGAGATCAAGCAATTGCTAACTATGACCAAAGCAGTGTGGTCCTTCCTAGCAATATAGTCATGGGGTTGAACCAATTCTCACAAAAGCCTAGCAGGACTTCAGTGATCAGCATGGATGGTAGTAGCATCAACACGCAGATAGTTCGACATTCAATGTCTGAACTATTGACTTCTAAAGCCGGCACCTCTATGTTTTCTTCCAAAAGGAAGAGTTGTGGGGTTAAAAGTGAAGAAGCCCCGTGTCTAGCCTCTACTGGTAAATGTCATTGCTCAAAGAGAAGGAAACTAAGAATAAAGAGAAGAATCCGAGTTCCGGCTGTAAGCAACCGGCTAGCAGATATACCTCCTGATGACTATTCTTGGAGGAAGTATGGGCAGAAACCTATTAAAGGATCTCCATATCCTAGGAGTTACTACAAATGCAGCAGTGTAAGGGGATGTCCTGCAAGAAAACATGTCGAAAGATGTCTTGAGGATTCTACCATGTTGGTTGTTACTTATGAAGGAGATCATCAACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plant_zn_clust WRKY

Protein Analysis

290

Amino Acids

32.43

Weight (kDa)

9.52

Isoelectric Point (pI)

56.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_zn_clust PF10533 185 - 229 2.4e-14 Plant zinc cluster domain
WRKY PF03106 233 - 289 2.7e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016930)

Species Orthologous Gene IDs
malus_domestica MD06G1062800.v1.1
prunus_persica Prupe.5G074200_v2.0.a1
pyrus_communis pycom06g06060
rosa_chinensis RchiOBHm_Chr7g0202671
rosa_laevigata RLG00000003582
rosa_roxburghii Rroxscaffold_3G00254420
rosa_rugosa Rorug07G0071700 Rorug07G0071800 Rorug07G0071900
rosa_samantha Rh7AG199600 Rh7BG200400 Rh7CG211100 Rh7DG206500
rosa_wichuraiana Rw7G017480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 530
AccII CGCG 1 cut(s) 59
AclWI GGATC 3 cut(s) 184, 209, 748
AcsI RAATTY 2 cut(s) 4, 146
AcuI CTGAAG 2 cut(s) 329, 432
AfiI CCNNNNNNNGG 2 cut(s) 661, 737
AflIII ACRYGT 1 cut(s) 802
AgsI TTSAA 5 cut(s) 22, 53, 110, 418, 501
AhlI ACTAGT 1 cut(s) 44
AjnI CCWGG 1 cut(s) 240
AjuI GAANNNNNNNTTGG 1 cut(s) 28
AleI CACNNNNGTG 1 cut(s) 300
AluBI AGCT 1 cut(s) 339
AluI AGCT 1 cut(s) 339
Alw21I GWGCWC 1 cut(s) 307
AlwI GGATC 3 cut(s) 184, 209, 748
ApeKI GCWGC 1 cut(s) 771
ApoI RAATTY 2 cut(s) 4, 146
Asp700I GAANNNNTTC 2 cut(s) 146, 422
AspA2I CCTAGG 1 cut(s) 752
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 3 cut(s) 212, 256, 388
AsuNHI GCTAGC 1 cut(s) 679
AvaII GGWCC 3 cut(s) 212, 256, 388
AvrII CCTAGG 1 cut(s) 752
BanI GGYRCC 1 cut(s) 530
BbsI GAAGAC 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 783
BccI CCATC 3 cut(s) 167, 247, 458
BcgI CGANNNNNNTGC 2 cut(s) 473, 507
BciT130I CCWGG 1 cut(s) 242
BclI TGATCA 1 cut(s) 453
BcuI ACTAGT 1 cut(s) 44
BfaI CTAG 7 cut(s) 45, 68, 396, 438, 593, 680, 753
BisI GCNGC 1 cut(s) 772
BlnI CCTAGG 1 cut(s) 752
BlsI GCNGC 1 cut(s) 773
Bme1390I CCNGG 1 cut(s) 242
Bme18I GGWCC 3 cut(s) 212, 256, 388
BmgT120I GGNCC 3 cut(s) 212, 256, 388
BmiI GGNNCC 2 cut(s) 213, 532
BmrFI CCNGG 1 cut(s) 242
BmsI GCATC 2 cut(s) 124, 483
BmtI GCTAGC 1 cut(s) 683
BoxI GACNNNNGTC 1 cut(s) 261
BpiI GAAGAC 1 cut(s) 41
BpuEI CTTGAG 2 cut(s) 114, 839
BsaJI CCNNGG 1 cut(s) 752
Bsc4I CCNNNNNNNGG 2 cut(s) 661, 737
Bse118I RCCGGY 2 cut(s) 527, 675
Bse1I ACTGG 2 cut(s) 228, 607
Bse3DI GCAATG 1 cut(s) 613
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 1 cut(s) 752
BseGI GGATG 3 cut(s) 250, 469, 791
BseLI CCNNNNNNNGG 2 cut(s) 661, 737
BseMI GCAATG 1 cut(s) 613
BseNI ACTGG 2 cut(s) 228, 607
BseXI GCAGC 1 cut(s) 783
Bsh1236I CGCG 1 cut(s) 59
Bsh1285I CGRYCG 1 cut(s) 301
BshNI GGYRCC 1 cut(s) 530
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 3 cut(s) 528, 662, 676
BslI CCNNNNNNNGG 2 cut(s) 661, 737
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 6 cut(s) 176, 201, 352, 453, 740, 856
BspFNI CGCG 1 cut(s) 59
BspLI GGNNCC 2 cut(s) 213, 532
BspOI GCTAGC 1 cut(s) 683
BspPI GGATC 3 cut(s) 184, 209, 748
BspT107I GGYRCC 1 cut(s) 530
BsrDI GCAATG 1 cut(s) 613
BsrFI RCCGGY 2 cut(s) 527, 675
BsrI ACTGG 2 cut(s) 228, 607
BssAI RCCGGY 2 cut(s) 527, 675
BssECI CCNNGG 1 cut(s) 752
BssMI GATC 6 cut(s) 176, 201, 352, 453, 740, 856
BssT1I CCWWGG 1 cut(s) 752
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 1 cut(s) 302
Bst6I CTCTTC 1 cut(s) 551
BstC8I GCNNGC 2 cut(s) 529, 681
BstDEI CTNAG 2 cut(s) 260, 635
BstENI CCTNNNNNAGG 1 cut(s) 735
BstF5I GGATG 3 cut(s) 250, 469, 791
BstFNI CGCG 1 cut(s) 59
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 6 cut(s) 179, 204, 355, 456, 743, 859
BstMBI GATC 6 cut(s) 176, 201, 352, 453, 740, 856
BstMCI CGRYCG 1 cut(s) 301
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 1 cut(s) 242
BstNSI RCATGY 1 cut(s) 806
BstPAI GACNNNNGTC 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 240
BstUI CGCG 1 cut(s) 59
BstV1I GCAGC 1 cut(s) 783
BstV2I GAAGAC 1 cut(s) 41
BstX2I RGATCY 1 cut(s) 740
BstYI RGATCY 1 cut(s) 740
BtsCI GGATG 3 cut(s) 250, 469, 791
BtsI GCAGTG 3 cut(s) 144, 388, 781
BtsIMutI CAGTG 5 cut(s) 144, 235, 388, 456, 781
Cac8I GCNNGC 2 cut(s) 529, 681
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 2 cut(s) 527, 675
Cfr13I GGNCC 3 cut(s) 212, 256, 388
CviAII CATG 5 cut(s) 29, 409, 460, 803, 832
CviJI RGCY 9 cut(s) 120, 313, 339, 436, 527, 584, 596, 665, 679
CviKI_1 RGCY 9 cut(s) 120, 313, 339, 436, 527, 584, 596, 665, 679
DdeI CTNAG 2 cut(s) 260, 635
DpnI GATC 6 cut(s) 178, 203, 354, 455, 742, 858
DpnII GATC 6 cut(s) 176, 201, 352, 453, 740, 856
Eam1104I CTCTTC 1 cut(s) 551
EarI CTCTTC 1 cut(s) 551
Eco130I CCWWGG 1 cut(s) 752
Eco47I GGWCC 3 cut(s) 212, 256, 388
Eco57I CTGAAG 2 cut(s) 329, 432
EcoNI CCTNNNNNAGG 1 cut(s) 735
EcoO109I RGGNCCY 1 cut(s) 212
EcoRI GAATTC 2 cut(s) 4, 146
EcoRII CCWGG 1 cut(s) 240
EcoT14I CCWWGG 1 cut(s) 752
ErhI CCWWGG 1 cut(s) 752
FaeI CATG 5 cut(s) 32, 412, 463, 806, 835
FalI AAGNNNNNCTT 2 cut(s) 105, 137
FatI CATG 5 cut(s) 28, 408, 459, 802, 831
FbaI TGATCA 1 cut(s) 453
Fnu4HI GCNGC 1 cut(s) 772
FokI GGATG 3 cut(s) 257, 476, 798
Fsp4HI GCNGC 1 cut(s) 772
FspBI CTAG 7 cut(s) 45, 68, 396, 438, 593, 680, 753
GlaI GCGC 1 cut(s) 60
GluI GCNGC 1 cut(s) 772
HapII CCGG 3 cut(s) 528, 662, 676
HhaI GCGC 1 cut(s) 61
Hin1II CATG 5 cut(s) 32, 412, 463, 806, 835
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HinfI GANTC 2 cut(s) 651, 824
HpaII CCGG 3 cut(s) 528, 662, 676
Hpy188I TCNGA 6 cut(s) 85, 152, 206, 348, 508, 656
Hpy188III TCNNGA 4 cut(s) 110, 131, 695, 818
HpyAV CCTTC 5 cut(s) 136, 353, 401, 621, 845
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4V TGCA 3 cut(s) 137, 771, 794
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 2 cut(s) 260, 635
Hsp92II CATG 5 cut(s) 32, 412, 463, 806, 835
HspAI GCGC 1 cut(s) 59
KroI GCCGGC 1 cut(s) 527
KroNI GCCGGC 1 cut(s) 529
Ksp22I TGATCA 1 cut(s) 453
Kzo9I GATC 6 cut(s) 176, 201, 352, 453, 740, 856
LmnI GCTCC 1 cut(s) 344
Lsp1109I GCAGC 1 cut(s) 783
LweI GCATC 2 cut(s) 124, 483
MaeI CTAG 7 cut(s) 45, 68, 396, 438, 593, 680, 753
MaeIII GTNAC 4 cut(s) 230, 322, 758, 841
MalI GATC 6 cut(s) 178, 203, 354, 455, 742, 858
MboI GATC 6 cut(s) 176, 201, 352, 453, 740, 856
MboII GAAGA 6 cut(s) 41, 267, 537, 568, 590, 660
MfeI CAATTG 1 cut(s) 360
MflI RGATCY 1 cut(s) 740
MhlI GDGCHC 1 cut(s) 307
MluCI AATT 5 cut(s) 4, 146, 268, 360, 422
MnlI CCTC 7 cut(s) 25, 192, 544, 607, 702, 705, 814
MroNI GCCGGC 1 cut(s) 527
MroXI GAANNNNTTC 2 cut(s) 146, 422
MseI TTAA 2 cut(s) 570, 735
MslI CAYNNNNRTG 2 cut(s) 300, 536
MspI CCGG 3 cut(s) 528, 662, 676
MspR9I CCNGG 1 cut(s) 242
MunI CAATTG 1 cut(s) 360
MvaI CCWGG 1 cut(s) 242
MvnI CGCG 1 cut(s) 59
MwoI GCNNNNNNNGC 1 cut(s) 134
NaeI GCCGGC 1 cut(s) 529
NdeII GATC 6 cut(s) 176, 201, 352, 453, 740, 856
NgoMIV GCCGGC 1 cut(s) 527
NheI GCTAGC 1 cut(s) 679
NlaIII CATG 5 cut(s) 32, 412, 463, 806, 835
NlaIV GGNNCC 2 cut(s) 213, 532
NmuCI GTSAC 1 cut(s) 230
NspI RCATGY 1 cut(s) 806
OliI CACNNNNGTG 1 cut(s) 300
PciI ACATGT 1 cut(s) 802
PdiI GCCGGC 1 cut(s) 529
PdmI GAANNNNTTC 2 cut(s) 146, 422
PfeI GAWTC 2 cut(s) 651, 824
PkrI GCNGC 1 cut(s) 773
PpuMI RGGWCCY 1 cut(s) 212
PscI ACATGT 1 cut(s) 802
PshAI GACNNNNGTC 1 cut(s) 261
Psp5II RGGWCCY 1 cut(s) 212
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PspN4I GGNNCC 2 cut(s) 213, 532
PspPI GGNCC 3 cut(s) 212, 256, 388
PspPPI RGGWCCY 1 cut(s) 212
PsuI RGATCY 1 cut(s) 740
RseI CAYNNNNRTG 2 cut(s) 300, 536
SaqAI TTAA 2 cut(s) 570, 735
SatI GCNGC 1 cut(s) 772
Sau3AI GATC 6 cut(s) 176, 201, 352, 453, 740, 856
Sau96I GGNCC 3 cut(s) 212, 256, 388
ScrFI CCNGG 1 cut(s) 242
SduI GDGCHC 1 cut(s) 307
SetI ASST 7 cut(s) 36, 69, 261, 341, 536, 694, 733
SfaNI GCATC 2 cut(s) 124, 483
SinI GGWCC 3 cut(s) 212, 256, 388
SmiMI CAYNNNNRTG 2 cut(s) 300, 536
SmlI CTYRAG 2 cut(s) 129, 818
SmoI CTYRAG 2 cut(s) 129, 818
SpeI ACTAGT 1 cut(s) 44
Sse9I AATT 5 cut(s) 4, 146, 268, 360, 422
SspMI CTAG 7 cut(s) 45, 68, 396, 438, 593, 680, 753
StyD4I CCNGG 1 cut(s) 240
StyI CCWWGG 1 cut(s) 752
TaaI ACNGT 1 cut(s) 302
TaqI TCGA 2 cut(s) 493, 807
TasI AATT 5 cut(s) 4, 146, 268, 360, 422
TfiI GAWTC 2 cut(s) 651, 824
Tru1I TTAA 2 cut(s) 570, 735
Tru9I TTAA 2 cut(s) 570, 735
TscAI CASTG 5 cut(s) 144, 235, 388, 456, 781
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 1 cut(s) 771
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 4 cut(s) 17, 261, 267, 864
TspRI CASTG 5 cut(s) 144, 235, 388, 456, 781
VpaK11BI GGWCC 3 cut(s) 212, 256, 388
XagI CCTNNNNNAGG 1 cut(s) 735
XapI RAATTY 2 cut(s) 4, 146
XceI RCATGY 1 cut(s) 806
XmaJI CCTAGG 1 cut(s) 752
XmnI GAANNNNTTC 2 cut(s) 146, 422
XspI CTAG 7 cut(s) 45, 68, 396, 438, 593, 680, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.